!"!"
6: (# 3 5
F"
#)M89 β" 1
#)M&99 (#" (##C )(";
' &"!!" 5 ' &"!#>!" 5 ' &" !@>!" 5#)M89 )*)7
8 . 5
3
#>#.
F" ' &" 5-O9998/' &"!@>!" 5 (# #>#."
- O9998/ ' &"!#>!" 5 @' =B ' &"!!" 5 -O998/ β"
3 ' #>#."
' &" 5-O9998/. 0!@>
@' =B-O998/' &"!#>!" 5 ' &"!!" 5 ' &"!@>!" 5 3 (# #>#."
- O9998/ ' &" !@>!" 5
3 59" ' &" 5 *
β"
)("; ' &"!!" ' &"!#>!" ' &"!@>!" 5 (*)+()7 0!@>
(##C 3 3 (# 2
3
Ad5-lacZ
RAd36
Ad5-PPlacZ 24.0±1.0
Ad5-PMEP- Ad5-PREP- p lacZ lacZ (ANOVA) 12.2±0.5 36.6±2.0 <0.0001
Proportion of SMC 1.0±0.1 13.4±1.0 expressing lacZ (%) β-gal. activity in 0.01±0.005 0.178±0.04 0.427±0.02 0.419±0.06 0.865±0.12 <0.0001 SMC lysates (IU/mg protein/ml) β-gal. activity in — n/a — — n/a -— 0.038±0.004 0.051±0.002 0.056±0.006 0.10 COS-7 lysates (IU/mg protein/ml)
652. Expression and Regulation of the Coxsackie- and Adenovirus Receptor (CAR) in Premalignant Lesions and Advanced Neoplasms M. Anders,1 C. Christian,1 R. Warren,1 A. Balmain,1 M. J. Bissell,2 F. McCormick,1 W. M. Korn.1 1 Comprehensive Cancer Center, University of California, San Francisco, CA, United States; 2Lawrence Berkeley National Laboratories, Berkeley, CA, United States.
3 " ' @ -'@/ '
3
- /
3 '@
0 3
'@ 3 $(
1 '@ 3
'@ 3
'@ 3
3 '@
"
0 '@
3
@"8%#'!A '@ 3
" 3 '@
" 0 "
? E '@
3
2
G
S214
653. Combining T ransductional-T argeting with Transductional-T ransductional-Targeting Translational-T argeting for Prostate Cancer Adenoviralranslational-Targeting Mediated Oncolytic Effect Thomas A. Gardner,1 Sudhanshu P. Raikwar,2 Victor Krasnykh,1 Chinghai Kao.1 1 Dept of Urology, Microbiology and Immunology, Walther Oncology Center, Indiana University Medical Center, Indianapolis, IN, United States; 2Division of Human Gene Therapy, University of Alabama at Birmingham, Birmingham, AL, United States.
3
3
)
@$ "
' @$
4@$
@' ?")>8'
@' )>8'!
-D*! 6", !"=/ -@",5/
&389& % 3 ,6
98 8 % 6 899 &99
' 6
,
, &
&
1 ,5= )
D*! 6",
8 % @' )>8'
6",
D*! *
!"= @",5 2 & ;9H 6",
98
899H
8 % 3
@' ?")>8' @$
6",
@' ")>8'
59H !> 899H !> 98% 8 %
D*!
@' ?")>8' 89H 3
98 % B9H D*! 3
8 % ? 1 !>
@$
$ A $
G
3
654. Reduction of Adenoviral Natural T ropism to Liver Tropism by Both Ablation of Fiber-CAR Interaction and Use of Replaceable Short Fiber Takafumi Nakamura,1,2 Kenzo Sato,2 Hirofumi Hamada.1 1 Department of Molecular Medicine, Sapporo Medical University, Sapporo, Japan; 2Department of Molecular Biology, Tottori University, Yonago, Japan. -' /
3 -'@/ '
69 -' 69/ I G '@ '@ 0
& -' &/"
7 ?%69(D
' & ' 69 ?%69(
' & ' 69 ?%&69(
Molecular Therapy Vol. 5, No. 5, May 2002, Part 2 of 2 Parts Copyright © The American Society of Gene Therapy