652. Expression and Regulation of the Coxsackie- and Adenovirus Receptor (CAR) in Premalignant Lesions and Advanced Neoplasms

652. Expression and Regulation of the Coxsackie- and Adenovirus Receptor (CAR) in Premalignant Lesions and Advanced Neoplasms

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        Ad5-lacZ

RAd36

Ad5-PPlacZ 24.0±1.0

Ad5-PMEP- Ad5-PREP- p lacZ lacZ (ANOVA) 12.2±0.5 36.6±2.0 <0.0001

Proportion of SMC 1.0±0.1 13.4±1.0 expressing lacZ (%) β-gal. activity in 0.01±0.005 0.178±0.04 0.427±0.02 0.419±0.06 0.865±0.12 <0.0001 SMC lysates (IU/mg protein/ml) β-gal. activity in — n/a — — n/a -— 0.038±0.004 0.051±0.002 0.056±0.006 0.10 COS-7 lysates (IU/mg protein/ml)

652. Expression and Regulation of the Coxsackie- and Adenovirus Receptor (CAR) in Premalignant Lesions and Advanced Neoplasms M. Anders,1 C. Christian,1 R. Warren,1 A. Balmain,1 M. J. Bissell,2 F. McCormick,1 W. M. Korn.1 1 Comprehensive Cancer Center, University of California, San Francisco, CA, United States; 2Lawrence Berkeley National Laboratories, Berkeley, CA, United States.     

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653. Combining T ransductional-T argeting with Transductional-T ransductional-Targeting Translational-T argeting for Prostate Cancer Adenoviralranslational-Targeting Mediated Oncolytic Effect Thomas A. Gardner,1 Sudhanshu P. Raikwar,2 Victor Krasnykh,1 Chinghai Kao.1 1 Dept of Urology, Microbiology and Immunology, Walther Oncology Center, Indiana University Medical Center, Indianapolis, IN, United States; 2Division of Human Gene Therapy, University of Alabama at Birmingham, Birmingham, AL, United States.           

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654. Reduction of Adenoviral Natural T ropism to Liver Tropism by Both Ablation of Fiber-CAR Interaction and Use of Replaceable Short Fiber Takafumi Nakamura,1,2 Kenzo Sato,2 Hirofumi Hamada.1 1 Department of Molecular Medicine, Sapporo Medical University, Sapporo, Japan; 2Department of Molecular Biology, Tottori University, Yonago, Japan.               -' /  

         



     

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    Molecular Therapy Vol. 5, No. 5, May 2002, Part 2 of 2 Parts Copyright © The American Society of Gene Therapy