- Email: [email protected]
659 VITAMIN B12 DEFICIENCY IN CHILDREN AFTER CONTINENT URINARY DIVERSION BY MAINZ POUCH TECHNIQUE
SDWLHQWVRXWRIFKLOGUHQZLWKYHVLFRXUHWHUDOUHᚐX[DQWLFKROLQHUJLFDJHQWVVXFK DVR[\EXW\QLQHWROWHURGLQHRUVROLIHQDFLQHZHUHDSSOLHG Results: 5HᚐX[GLVDSSHDUHGLQFKLOGUHQZKLOHLQ ZDVREVHUYHGLWVUHGXFWLRQ 7KH FRQVHUYDWLYH WUHDWPHQW ZLWK &,& DQG DQWLFKROLQHUJLF DJHQWV SURYHG HᚎHFWLYHQHVVLQFKLOGUHQEXWUHYHDOHGWREHLQHᚎHFWLYHLQ,QFKLOGUHQRXW RIZLWKHᚎHFWLYHFRQVHUYDWLYHWUHDWPHQWUHᚐX[GLVDSSHDUHGZKLOHWKHGHFUHDVH RI GHJUHH RI UHᚐX[ ZDV REVHUYHG LQ LQ ERWK ZLWK VLPXOWDQHRXV UHGXFWLRQ RI GHWUXVRUSUHVVXUH7KHLQMHFWLRQRIEXONLQJDJHQWVZDVXVHGZLWKJRRGUHVXOWVLQ WKHVHFDVHV,QSDWLHQWVZLWKRXWFKDQJHVRIGHJUHHRIUHᚐX[DQGOHYHORIGHWUXVRU SUHVVXUHZHUHXVHGGLᚎHUHQWPHWKRGVRIEODGGHUDXJPHQWDWLRQ$XWRDXJPHQWDWLRQ F\VWRSODVW\ZDVDSSOLHGLQSDWLHQWVLOHRF\VWRSODVW\LQDQGFRORF\VWRSODVW\LQ FKLOGUHQ,QDXWRDXJPHQWDWLRQJURXSUHᚐX[GLVDSSHDUHGLQDQGLWGHFUHDVHG WRWKLUGGHJUHHLQFKLOGUHQ FKLOGUHQIURPLOHRF\VWRSODVW\JURXSGHPRQVWUDWHG WKHGLVDSSHDULQJRIUHᚐX[ZKLOHWKHUHGXFWLRQRIGHJUHHRIUHᚐX[ZDVREVHUYHGLQ RWKHU,QWKHSDWLHQWVZLWKFRORF\VWRSODVW\UHᚐX[HVGLVDSSHDUHGLQFDVHVLQ RWKHUUHGXFHGWKHGHJUHHWRVHFRQGRUWKLUG$OOSDWLHQWVLQZKRPWKHGHFUHDVHRI GHJUHHRIUHᚐX[ZDVREVHUYHGZHUHTXDOLᚏHGWRLQMHFWLRQRIEXONLQJDJHQWV Conclusions: $GHTXDWHO\ TXLFN UHGXFLQJ RI DEQRUPDO KLJK GHWUXVRU SUHVVXUH LV VXᚑFLHQW IRU UHᚐX[ GLVDSSHDUDQFH RU WR UHGXFWLRQ RI LWV GHJUHH 2XU DWWHQWLRQ VKRXOG EH SDLG WR HDUO\ EHJLQQLQJ RI SURSHU WUHDWPHQW DFFRUGLQJ WR FRQGLWLRQ RI XSSHU XULQDU\ WUDFW OHYHO RI DEQRUPDO GHWUXVRU SUHVVXUH DQG EODGGHU YROXPH ,QHᚎHFWLYHFRQVHUYDWLYHWUHDWPHQWVKRXOGEHLPPHGLDWHO\FKDQJHGDIWHUVKRUWWLPH RIXURG\QDPLFREVHUYDWLRQV
659
VITAMIN B12 DEFICIENCY IN CHILDREN AFTER CONTINENT URINARY DIVERSION BY MAINZ POUCH TECHNIQUE
6WHLQ56FKU¸GHU$'DYLV.7K¾URᚎ-: University Medical Center of The Johannes Gutenberg University Mainz, Mainz, Germany Introduction & Objectives: 0$,1= SRXFK , XULQDU\ GLYHUVLRQ EHLQJ D PL[HG DXJPHQWDWLRQ RI LOHXP DQG FRHFXP SURYLGHV D KLJKFDSDFLW\ ORZSUHVVXUH UHVHUYRLU7KHXVHRIWHUPLQDOLOHXPFDQFDXVHDGHFUHDVHRIYLWDPLQ%OHYHOV 6\PSWRPVFDXVHGE\YLWDPLQ%GHᚏFLHQF\DUHUDUHEXWVRPHDUHLUUHYHUVLEOH WKHUHIRUHDQQXDOFKHFNXSVDUHUHFRPPHQGHG,QWKLVVWXG\WKHYLWDPLQ%OHYHOV LQWKHDERYHSDWLHQWVZHUHDQDO\]HGLQUHWURVSHFW Material & Methods: 7KH LOHRFHFDO VHJPHQW ZDV XVHG IRU XULQDU\ WUDFW UHFRQVWUXFWLRQLQ!FKLOGUHQZKRื\HDUVRIDJH2XWRISDWLHQWVUHFHLYHG DQLOHRFHFDOEODGGHUVXEVWLWXWLRQWKHUHPDLQLQJXQGHUZHQWFRQWLQHQWFXWDQHRXV GLYHUVLRQ9LWDPLQ%OHYHOVZHUHGHWHUPLQHGDWYDULRXVWLPHSRLQWVZLWKLQXSWR \HDUVDIWHUVXUJHU\9LWDPLQ%YDOXHVEHORZSJPOZHUHFRQVLGHUHGORZ DQGVXEVWLWXWLRQWKHUDS\ZDVUHFRPPHQGHG9DOXHVDIWHUVXEVWLWXWLRQWKHUDS\ZHUH H[FOXGHGIURPIXUWKHUDQDO\VLV Results: $WRWDORIYLWDPLQ%OHYHOVZHUHGHWHUPLQHGLQSDWLHQWVRYHUDQ DYHUDJHRI\HDUVSRVWRSHUDWLYHO\ \HDUV ,QRIWKHVHSDWLHQWVWKUHHRU PRUH UDQJHPHGLDQ YLWDPLQ%YDOXHVZHUHDYDLODEOHIRUDQDO\VLV,Q SDWLHQWVWKHYDOXHVRQO\VKRZHGVRPHXQGXODWLRQRUHYHQDQLQFUHDVHRYHU WLPH PHGLDQ ,QWKHUHPDLQLQJSDWLHQWVDJHQHUDOGHFUHDVH PHGLDQ ZDV REVHUYHG RI SDWLHQWV ZHUH LQ QHHG RI YLWDPLQ%VXSSOHPHQWDWLRQ2YHUDOODFRQWLQXRXVVORZGHFUHDVHRIYLWDPLQ% YDOXHVZDVREVHUYHGRYHUWKHIROORZXSSHULRGRI\HDUVLQWKHPDMRULW\RIWKH SDWLHQWV Conclusions: 3DWLHQWVLQZKRPWKHLOHRFHFDOVHJPHQWZDVXVHGIRUUHFRQVWUXFWLRQ RIWKHXULQDU\WUDFWLQFKLOGKRRGDUHDWULVNIRUGHYHORSPHQWRIYLWDPLQ%GHᚏFLHQF\ 7KHUHLVDZLGHYDULDWLRQRIWKHYLWDPLQ%OHYHOVRYHUWLPHKRZHYHUDGHFUHDVH EHORZSJPOKDVWREHFRQVLGHUHGUHOHYDQWDQGVXSSOHPHQWDWLRQLVQHHGHG,Q RXUSDWLHQWVZHREVHUYHGDVORZEXWSHUPDQHQWGHFUHDVHRIYLWDPLQ%OHYHOVLQ PRUHWKDQRIWKHSDWLHQWVXQGHUZHQWVXEVWLWXWLRQRIYLWDPLQ%$QQXDO VHUXPYLWDPLQ%OHYHOVGHWHUPLQDWLRQKDVWREHUHFRPPHQGHGEHJLQQLQJ\HDUV SRVWRSHUDWLYHO\
660
HALF-CENTURY FOLLOW-UP AFTER COFFEY DIVERSION IN EARLY CHILDHOOD
-RQVVRQ2.$EUDKDPVVRQ.$QGHUVVRQ&3HWWHUVVRQ66LOO«Q8 1 Sahlgrenska University Hospital, Dept. of Urology, Göteborg, Sweden, 2Queen Silvia Children’s Hospital, Dept. of Paediatric Urology, Göteborg, Sweden Introduction & Objectives: 7RDQDO\VHWKHFOLQLFDORXWFRPH\HDUVDIWHU&RᚎH\ GLYHUVLRQLQHDUO\FKLOGKRRG Material & Methods: ,QWKH&KLOGUHQಬV+RVSLWDO*¸WHERUJ6ZHGHQFKLOGUHQ ER\VJLUOV ZHUHRSHUDWHGRQZLWK&RᚎH\GLYHUVLRQEHWZHHQ DQG 0HDQDJH\HDUV 6'UDQJH\HDUV 'DWDDUHDYDLODEOHIRURIWKHVH SDWLHQWV7KHPRVWFRPPRQFDXVHIRU&RᚎH\GLYHUVLRQZDVEODGGHUH[WURSK\7KLV UHWURVSHFWLYHVWXG\ZDVEDVHGRQSDWLHQWᚏOHVDVZHOODVRQSHUVRQDOLQWHUYLHZV Results: SDWLHQWVDUHDOLYHZLWKDPHDQDJHRI \HDUV 6'UDQJH \HDUV )RXU SDWLHQWV KDYH GLHG RI FRORUHFWDO FDUFLQRPD DQG WZR RI UHDVRQV QRW UHODWHGWRWKH&RᚎH\GLYHUVLRQ7ZRRIWKHVXUYLYLQJSDWLHQWVGRVWLOOKDYHWKHLU &RᚎH\GHYLDWLRQDQG\HDUVDIWHUGLYHUVLRQDQGDUHYHU\VDWLVᚏHGZLWKLW2I WKHWRWDOJURXSRISDWLHQWV KDYHEHHQUHGLYHUWHGWRDQLQFRQWLQHQWFXWDQHRXV
Eur Urol Suppl 2010;9(2):218
GLYHUVLRQ SDWLHQWV RUDFRQWLQHQWFXWDQHRXVGLYHUVLRQ SDWLHQWV \HDUV DIWHU &RᚎH\ GLYHUVLRQ PHDQ 6' UDQJH \HDUV 7KH PRVW FRPPRQ UHDVRQVIRUUHGLYHUVLRQZHUHFRORUHFWDOFDUFLQRPD SDWLHQWV IHDURIFDUFLQRPD SDWLHQWV DQGIUHTXHQWS\HOLWLV SDWLHQWV +DOIRIWKHUHGLYHUWHGSDWLHQWVFODLPHG WKDW WKH\ ZHUH VDWLVᚏHG ZLWK WKH &RᚎH\ GLYHUVLRQ $ WRWDO RI VHYHQ SDWLHQWV SHUFHQW GHYHORSHGFRORUHFWDOFDUFLQRPD\HDUV PHDQ6'UDQJH \HDUV DIWHU&RᚎH\GLYHUVLRQ7KUHHRIWKHVHSDWLHQWVGHYHORSHGFDUFLQRPD DQG\HDUVDIWHUUHRSHUDWLRQIURP&RᚎH\WRDQRWKHUW\SHRIGLYHUVLRQ Conclusions: 0RVWSDWLHQWVGLYHUWHGLQFKLOGKRRGZLWKWKH&RᚎH\WHFKQLTXHUHDFK KLJKDJHVLQVSLWHRIVHYHUHFRQJHQLWDOPDOIRUPDWLRQV%\XVLQJWKLVWHFKQLTXHLW ZDVSRVVLEOHWRDYRLGH[WHUQDOXULQDU\VDPSOLQJGHYLFHVXQWLOWKHSDWLHQWVEHFDPH DGXOW,QVSLWHRIORRVHIUHTXHQWVWROHVDSSUR[LPDWHO\KDOIRIWKHSDWLHQWVFODLPHG WKDW WKH\ ZHUH VDWLVᚏHG ZLWK WKH &RᚎH\ GLYHUVLRQ 7KH PDLQ WKUHDW LV FRORUHFWDO FDUFLQRPD ZKLFK RFFXUV LQ RQH WKLUG RI WKH SDWLHQWV /LIHORQJ VFUHHQLQJ IRU FRORUHFWDOFDUFLQRPDLVQHFHVVDU\DOVRDIWHUUHPRYDORIWKH&RᚎH\GLYHUVLRQ
Poster Session 41 EXPERIMENTAL THERAPY AND DIAGNOSTICS IN PROSTATE CANCER Monday, 19 April, 09.15-10.45, Barcelona Room
661
APOPTOSIS OF HUMAN ANDROGEN-DEPENDENT PROSTATE CANCER LNCAP CELLS TREATED WITH ANDROGEN RECEPTOR ELEMENT DECOY OLIGONUCLEOTIDES
/LIHQJ< Fudan University Cancer Hospital, Dept. of Urology, Shanghai, China Introduction & Objectives: 7R VWXG\ ZKLFK GHFR\ KDV WKH VWURQJHVW HᚎHFW RQ LQKLELWLQJDQGDSRSWRVLVRISURVWDWHFDQFHUFHOOOLQH/1&D3DQGVLJQDOWUDQVGXFWLRQ FKDQJHVLQWKHFHOOV Material & Methods: $FFRUGLQJWR$5(,$5(,,VHTXHQFHDWWKHSURPRWHUUHJLRQ RIWKHKXPDQSURVWDWHVSHFLᚏFDQWLJHQ 36$ JHQH:HV\QWKHVL]HG$5(,$5(,, GHFR\PRGLᚏHGWKHPZLWKSDUWVXOIXUL]HGRUWRWDOVXOIXUL]HG:HGHWHFWHGNLQGV RI GHFR\ ZLWK '1$SURWHLQ LQWHUDFWLRQV E\ JHO VKLIW DVVD\ (06$ WKH QXFOHDU H[WUDFWZDVSUHSDUHGIURP/1&D3FHOOV7KHQWKHDQWLDQGURJHQHᚎHFWRIWKH$5( GHFR\ZDVVWXGLHGLQ/1&D3FHOOVWUDQVIHFWHGZLWKWKH$5(GHFR\E\/LSRIHFWLQ $IWHU KU LQFXEDWLRQ ZLWK 0 GLK\GURWHVWRVWHURQH '+7 FHOO YLDELOLW\ ZDV GHWHFWHGE\076DVVD\DSRSWRVLVZDVH[DPLQHGE\'1$IUDJPHQWDWLRQFRQIRFDO PLFURVFRS\ZDVXVHGWRREVHUYHFHOOPRUSKRORJ\FKDQJHVZHVWHUQEORWDQDO\VLV ZDVXVHGWRGHWHFW$NWS$NWDQG3SURWHLQFKDQJHV
Results: 7KH JHO VKLIW DVVD\ GHPRQVWUDWHG $5(, $5(,, ZLWK WRWDO VXOIXUL]HG KDYH PRUH VSHFLᚏF ELQGLQJ DELOLW\ WR WKH /1&D3 QXFOHDU SURWHLQ ZKLFK LV PRVW OLNHO\$5$IWHUWUDQVIHFWHGZLWK˩PRO/IRXUNLQGVRI$5(GHFR\IRUK076 DVVD\ VKRZHG &HOOXODU SUROLIHUDWLRQ DFWLYLWLHV FDQ EH LQKLELWHG E\ $5(,, GHFR\ ZLWKWRWDOVXOIXUL]HG 3 WKRXJKQRWLQWKHFHOOVWUDQVIHFWHGZLWKWKHRWKHUV FRPSDULQJZLWKWKHFRQWUROGHFR\2EVHUYLQJE\FRQIRFDOPLFURVFRS\WKHFHOOXODU PRUSKRORJ\ FKDQJHG D ORW LQ WKH $5(,, WRWDO VXOIXUL]HG GHFR\WUDQVIHFWHG FHOOV '1$ IUDJPHQWDWLRQ LQ WKH FHOOV WUDQVIHFWHG ZLWK WRWDO VXOIXUL]HG $5(,, GHFR\ VKRZHG ಯ'1$ ODGGHUರ SUHVHQWLQJ FKDUDFWHULVWLF FKDQJHV RI DSRSWRVLV :HVWHUQ EORW DQDO\VLV VKRZ D VLJQLᚏFDQW UHGXFWLRQ RI S$NW DQG 3 SURWHLQ LQ WKH FHOOV WUDQVIHFWHGZLWKWRWDOVXOIXUL]HG$5(,,GHFR\DVFRPSDUHZLWKWKRVHRI/1&D3 EXW$NWSURWHLQQRWFKDQJHG Conclusions: 7KHWRWDOVXOIXUL]HG$5(,,GHFR\KDGDQDQWLDQGURJHQHᚎHFWDQG LQGXFHGDSRSWRVLVLQ/1&D3FHOOVE\UHGXFLQJWKHS$NWDQG3SURWHLQH[SUHVVLRQ VXEVHTXHQWO\FKDQJLQJVLJQDOWUDQVGXFWLRQLQ/1&D37KLVWRWDOVXOIXUL]HG$5(,, GHFR\PD\EHFRPHDSRWHQWLDOWKHUDSHXWLFWRROIRUSURVWDWHFDQFHUV
662
SIMVASTATIN INDUCES APOPTOSIS IN ANDROGENINDEPENDENT PROSTATE CANCER THROUGH A NUCLEAR FACTOR-κB-DEPENDENT MECHANISM
3DUN<+/HH(3DUN-+/HH&/HH6%.X-+-HRQJ+.ZDN& .LP++ Seoul National University, Dept. of Urology, Seoul, South Korea Introduction & Objectives: 5HFHQWO\ VWDWLQV KDYH EHHQ EHLQJ VWXGLHG IRU WKHLU
659
VITAMIN B12 DEFICIENCY IN CHILDREN AFTER CONTINENT URINARY DIVERSION BY MAINZ POUCH TECHNIQUE
6WHLQ56FKU¸GHU$'DYLV.7K¾URᚎ-: University Medical Center of The Johannes Gutenberg University Mainz, Mainz, Germany Introduction & Objectives: 0$,1= SRXFK , XULQDU\ GLYHUVLRQ EHLQJ D PL[HG DXJPHQWDWLRQ RI LOHXP DQG FRHFXP SURYLGHV D KLJKFDSDFLW\ ORZSUHVVXUH UHVHUYRLU7KHXVHRIWHUPLQDOLOHXPFDQFDXVHDGHFUHDVHRIYLWDPLQ%OHYHOV 6\PSWRPVFDXVHGE\YLWDPLQ%GHᚏFLHQF\DUHUDUHEXWVRPHDUHLUUHYHUVLEOH WKHUHIRUHDQQXDOFKHFNXSVDUHUHFRPPHQGHG,QWKLVVWXG\WKHYLWDPLQ%OHYHOV LQWKHDERYHSDWLHQWVZHUHDQDO\]HGLQUHWURVSHFW Material & Methods: 7KH LOHRFHFDO VHJPHQW ZDV XVHG IRU XULQDU\ WUDFW UHFRQVWUXFWLRQLQ!FKLOGUHQZKRื\HDUVRIDJH2XWRISDWLHQWVUHFHLYHG DQLOHRFHFDOEODGGHUVXEVWLWXWLRQWKHUHPDLQLQJXQGHUZHQWFRQWLQHQWFXWDQHRXV GLYHUVLRQ9LWDPLQ%OHYHOVZHUHGHWHUPLQHGDWYDULRXVWLPHSRLQWVZLWKLQXSWR \HDUVDIWHUVXUJHU\9LWDPLQ%YDOXHVEHORZSJPOZHUHFRQVLGHUHGORZ DQGVXEVWLWXWLRQWKHUDS\ZDVUHFRPPHQGHG9DOXHVDIWHUVXEVWLWXWLRQWKHUDS\ZHUH H[FOXGHGIURPIXUWKHUDQDO\VLV Results: $WRWDORIYLWDPLQ%OHYHOVZHUHGHWHUPLQHGLQSDWLHQWVRYHUDQ DYHUDJHRI\HDUVSRVWRSHUDWLYHO\ \HDUV ,QRIWKHVHSDWLHQWVWKUHHRU PRUH UDQJHPHGLDQ YLWDPLQ%YDOXHVZHUHDYDLODEOHIRUDQDO\VLV,Q SDWLHQWVWKHYDOXHVRQO\VKRZHGVRPHXQGXODWLRQRUHYHQDQLQFUHDVHRYHU WLPH PHGLDQ ,QWKHUHPDLQLQJSDWLHQWVDJHQHUDOGHFUHDVH PHGLDQ ZDV REVHUYHG RI SDWLHQWV ZHUH LQ QHHG RI YLWDPLQ%VXSSOHPHQWDWLRQ2YHUDOODFRQWLQXRXVVORZGHFUHDVHRIYLWDPLQ% YDOXHVZDVREVHUYHGRYHUWKHIROORZXSSHULRGRI\HDUVLQWKHPDMRULW\RIWKH SDWLHQWV Conclusions: 3DWLHQWVLQZKRPWKHLOHRFHFDOVHJPHQWZDVXVHGIRUUHFRQVWUXFWLRQ RIWKHXULQDU\WUDFWLQFKLOGKRRGDUHDWULVNIRUGHYHORSPHQWRIYLWDPLQ%GHᚏFLHQF\ 7KHUHLVDZLGHYDULDWLRQRIWKHYLWDPLQ%OHYHOVRYHUWLPHKRZHYHUDGHFUHDVH EHORZSJPOKDVWREHFRQVLGHUHGUHOHYDQWDQGVXSSOHPHQWDWLRQLVQHHGHG,Q RXUSDWLHQWVZHREVHUYHGDVORZEXWSHUPDQHQWGHFUHDVHRIYLWDPLQ%OHYHOVLQ PRUHWKDQRIWKHSDWLHQWVXQGHUZHQWVXEVWLWXWLRQRIYLWDPLQ%$QQXDO VHUXPYLWDPLQ%OHYHOVGHWHUPLQDWLRQKDVWREHUHFRPPHQGHGEHJLQQLQJ\HDUV SRVWRSHUDWLYHO\
660
HALF-CENTURY FOLLOW-UP AFTER COFFEY DIVERSION IN EARLY CHILDHOOD
-RQVVRQ2.$EUDKDPVVRQ.$QGHUVVRQ&3HWWHUVVRQ66LOO«Q8 1 Sahlgrenska University Hospital, Dept. of Urology, Göteborg, Sweden, 2Queen Silvia Children’s Hospital, Dept. of Paediatric Urology, Göteborg, Sweden Introduction & Objectives: 7RDQDO\VHWKHFOLQLFDORXWFRPH\HDUVDIWHU&RᚎH\ GLYHUVLRQLQHDUO\FKLOGKRRG Material & Methods: ,QWKH&KLOGUHQಬV+RVSLWDO*¸WHERUJ6ZHGHQFKLOGUHQ ER\VJLUOV ZHUHRSHUDWHGRQZLWK&RᚎH\GLYHUVLRQEHWZHHQ DQG 0HDQDJH\HDUV 6'UDQJH\HDUV 'DWDDUHDYDLODEOHIRURIWKHVH SDWLHQWV7KHPRVWFRPPRQFDXVHIRU&RᚎH\GLYHUVLRQZDVEODGGHUH[WURSK\7KLV UHWURVSHFWLYHVWXG\ZDVEDVHGRQSDWLHQWᚏOHVDVZHOODVRQSHUVRQDOLQWHUYLHZV Results: SDWLHQWVDUHDOLYHZLWKDPHDQDJHRI \HDUV 6'UDQJH \HDUV )RXU SDWLHQWV KDYH GLHG RI FRORUHFWDO FDUFLQRPD DQG WZR RI UHDVRQV QRW UHODWHGWRWKH&RᚎH\GLYHUVLRQ7ZRRIWKHVXUYLYLQJSDWLHQWVGRVWLOOKDYHWKHLU &RᚎH\GHYLDWLRQDQG\HDUVDIWHUGLYHUVLRQDQGDUHYHU\VDWLVᚏHGZLWKLW2I WKHWRWDOJURXSRISDWLHQWV KDYHEHHQUHGLYHUWHGWRDQLQFRQWLQHQWFXWDQHRXV
Eur Urol Suppl 2010;9(2):218
GLYHUVLRQ SDWLHQWV RUDFRQWLQHQWFXWDQHRXVGLYHUVLRQ SDWLHQWV \HDUV DIWHU &RᚎH\ GLYHUVLRQ PHDQ 6' UDQJH \HDUV 7KH PRVW FRPPRQ UHDVRQVIRUUHGLYHUVLRQZHUHFRORUHFWDOFDUFLQRPD SDWLHQWV IHDURIFDUFLQRPD SDWLHQWV DQGIUHTXHQWS\HOLWLV SDWLHQWV +DOIRIWKHUHGLYHUWHGSDWLHQWVFODLPHG WKDW WKH\ ZHUH VDWLVᚏHG ZLWK WKH &RᚎH\ GLYHUVLRQ $ WRWDO RI VHYHQ SDWLHQWV SHUFHQW GHYHORSHGFRORUHFWDOFDUFLQRPD\HDUV PHDQ6'UDQJH \HDUV DIWHU&RᚎH\GLYHUVLRQ7KUHHRIWKHVHSDWLHQWVGHYHORSHGFDUFLQRPD DQG\HDUVDIWHUUHRSHUDWLRQIURP&RᚎH\WRDQRWKHUW\SHRIGLYHUVLRQ Conclusions: 0RVWSDWLHQWVGLYHUWHGLQFKLOGKRRGZLWKWKH&RᚎH\WHFKQLTXHUHDFK KLJKDJHVLQVSLWHRIVHYHUHFRQJHQLWDOPDOIRUPDWLRQV%\XVLQJWKLVWHFKQLTXHLW ZDVSRVVLEOHWRDYRLGH[WHUQDOXULQDU\VDPSOLQJGHYLFHVXQWLOWKHSDWLHQWVEHFDPH DGXOW,QVSLWHRIORRVHIUHTXHQWVWROHVDSSUR[LPDWHO\KDOIRIWKHSDWLHQWVFODLPHG WKDW WKH\ ZHUH VDWLVᚏHG ZLWK WKH &RᚎH\ GLYHUVLRQ 7KH PDLQ WKUHDW LV FRORUHFWDO FDUFLQRPD ZKLFK RFFXUV LQ RQH WKLUG RI WKH SDWLHQWV /LIHORQJ VFUHHQLQJ IRU FRORUHFWDOFDUFLQRPDLVQHFHVVDU\DOVRDIWHUUHPRYDORIWKH&RᚎH\GLYHUVLRQ
Poster Session 41 EXPERIMENTAL THERAPY AND DIAGNOSTICS IN PROSTATE CANCER Monday, 19 April, 09.15-10.45, Barcelona Room
661
APOPTOSIS OF HUMAN ANDROGEN-DEPENDENT PROSTATE CANCER LNCAP CELLS TREATED WITH ANDROGEN RECEPTOR ELEMENT DECOY OLIGONUCLEOTIDES
/LIHQJ< Fudan University Cancer Hospital, Dept. of Urology, Shanghai, China Introduction & Objectives: 7R VWXG\ ZKLFK GHFR\ KDV WKH VWURQJHVW HᚎHFW RQ LQKLELWLQJDQGDSRSWRVLVRISURVWDWHFDQFHUFHOOOLQH/1&D3DQGVLJQDOWUDQVGXFWLRQ FKDQJHVLQWKHFHOOV Material & Methods: $FFRUGLQJWR$5(,$5(,,VHTXHQFHDWWKHSURPRWHUUHJLRQ RIWKHKXPDQSURVWDWHVSHFLᚏFDQWLJHQ 36$ JHQH:HV\QWKHVL]HG$5(,$5(,, GHFR\PRGLᚏHGWKHPZLWKSDUWVXOIXUL]HGRUWRWDOVXOIXUL]HG:HGHWHFWHGNLQGV RI GHFR\ ZLWK '1$SURWHLQ LQWHUDFWLRQV E\ JHO VKLIW DVVD\ (06$ WKH QXFOHDU H[WUDFWZDVSUHSDUHGIURP/1&D3FHOOV7KHQWKHDQWLDQGURJHQHᚎHFWRIWKH$5( GHFR\ZDVVWXGLHGLQ/1&D3FHOOVWUDQVIHFWHGZLWKWKH$5(GHFR\E\/LSRIHFWLQ $IWHU KU LQFXEDWLRQ ZLWK 0 GLK\GURWHVWRVWHURQH '+7 FHOO YLDELOLW\ ZDV GHWHFWHGE\076DVVD\DSRSWRVLVZDVH[DPLQHGE\'1$IUDJPHQWDWLRQFRQIRFDO PLFURVFRS\ZDVXVHGWRREVHUYHFHOOPRUSKRORJ\FKDQJHVZHVWHUQEORWDQDO\VLV ZDVXVHGWRGHWHFW$NWS$NWDQG3SURWHLQFKDQJHV
Results: 7KH JHO VKLIW DVVD\ GHPRQVWUDWHG $5(, $5(,, ZLWK WRWDO VXOIXUL]HG KDYH PRUH VSHFLᚏF ELQGLQJ DELOLW\ WR WKH /1&D3 QXFOHDU SURWHLQ ZKLFK LV PRVW OLNHO\$5$IWHUWUDQVIHFWHGZLWK˩PRO/IRXUNLQGVRI$5(GHFR\IRUK076 DVVD\ VKRZHG &HOOXODU SUROLIHUDWLRQ DFWLYLWLHV FDQ EH LQKLELWHG E\ $5(,, GHFR\ ZLWKWRWDOVXOIXUL]HG 3 WKRXJKQRWLQWKHFHOOVWUDQVIHFWHGZLWKWKHRWKHUV FRPSDULQJZLWKWKHFRQWUROGHFR\2EVHUYLQJE\FRQIRFDOPLFURVFRS\WKHFHOOXODU PRUSKRORJ\ FKDQJHG D ORW LQ WKH $5(,, WRWDO VXOIXUL]HG GHFR\WUDQVIHFWHG FHOOV '1$ IUDJPHQWDWLRQ LQ WKH FHOOV WUDQVIHFWHG ZLWK WRWDO VXOIXUL]HG $5(,, GHFR\ VKRZHG ಯ'1$ ODGGHUರ SUHVHQWLQJ FKDUDFWHULVWLF FKDQJHV RI DSRSWRVLV :HVWHUQ EORW DQDO\VLV VKRZ D VLJQLᚏFDQW UHGXFWLRQ RI S$NW DQG 3 SURWHLQ LQ WKH FHOOV WUDQVIHFWHGZLWKWRWDOVXOIXUL]HG$5(,,GHFR\DVFRPSDUHZLWKWKRVHRI/1&D3 EXW$NWSURWHLQQRWFKDQJHG Conclusions: 7KHWRWDOVXOIXUL]HG$5(,,GHFR\KDGDQDQWLDQGURJHQHᚎHFWDQG LQGXFHGDSRSWRVLVLQ/1&D3FHOOVE\UHGXFLQJWKHS$NWDQG3SURWHLQH[SUHVVLRQ VXEVHTXHQWO\FKDQJLQJVLJQDOWUDQVGXFWLRQLQ/1&D37KLVWRWDOVXOIXUL]HG$5(,, GHFR\PD\EHFRPHDSRWHQWLDOWKHUDSHXWLFWRROIRUSURVWDWHFDQFHUV
662
SIMVASTATIN INDUCES APOPTOSIS IN ANDROGENINDEPENDENT PROSTATE CANCER THROUGH A NUCLEAR FACTOR-κB-DEPENDENT MECHANISM
3DUN<+/HH(3DUN-+/HH&/HH6%.X-+-HRQJ+.ZDN& .LP++ Seoul National University, Dept. of Urology, Seoul, South Korea Introduction & Objectives: 5HFHQWO\ VWDWLQV KDYH EHHQ EHLQJ VWXGLHG IRU WKHLU