66 COMPARISON OF PLASMA SEMINAL L-CARNITINE CONCENTRATIONS IN ASTHENOZOOSPERMIC, TERATOZOOSPERMIC, OLIGOZOOSPERMIC AND NECROZOOSPERMIC INFERTILE MEN

IMSI & Male Infertility body of each oocyte was observed 3, 4 and 5 hours after insemination. Only the oocytes that extruded the second polar body were regarded as fertilized. If less than 70% oocytes from one patient extruded the second polar body 5 h after insemination, the oocytes with only the first polar body would be performed early rescue ICSI. Conventional ICSI cycles and IVF cycles undergone normal fertilization were setted as control group. The second polar body extrusion result at different time point, fertilization result, high quality embryo rate, clinical pregnancy rate and implantation rate were collected for analysis. Results: There was no significant difference (P > 0.05) between early rescue ICSI and conventional ICSI cycles in the clinic pregnancy rate (62 of 154, 40.24%vs.59 of 152 38.82%), and there was also no significant difference (P > 0.05) among early rescue cycles, conventional ICSI cycles and normal fertilized IVF cycles in implant rate (86 of 346, 24.86% vs.77 of 335, 22.99% vs.111 of 455, 24.39%). The ICSI part of rescue cycles was significantly higher (P < 0.05) than IVF part of rescue cycles in fertilization rate (652 of 703, 92.75% vs. 369 of 450, 82.00%) and 2PN rate (562 of 703, 79.94% vs. 313 of 450, 69.56%). The high quality embryo rate of ICSI part of rescue cycles significantly lower (P < 0.05) compared to IVF part of rescue cycles, conventional ICSI cycles and normal IVF cycles (272 of 562, 48.40% vs. 181 of 313, 57.83% vs. 725 of 1221, 59.38% vs. 1830 of 2851, 64.19%). And there was also significant difference (p < 0.05) between IVF part of rescue cycles and normal IVF cycles in high quality embryo rate (181 of 313, 57.83% vs. 830 of 2851, 64.19%). Only 5.97% (108/1810), 21.22% (384/1810) and 26.90% (487/1810) of oocytes in rescue cycles extrude second polar body 3 h, 4 h and 5 h after insemination respectively, significantly lower (P < 0.01) than the rate in normal IVF cycles (54.15%, 85.90% and 94.62% respectively). To the 43 cycles that no oocytes extruded second polar body 4 h after insemination, rescue ICSI be applied to all MII oocytes in 40(90.02%) cycles and part of MII oocytes in 43(100%) cycles. Rescue ICSI be applied to all MII oocytes in 40(100%) cycles that no oocytes extruded second polar body 5 h after insemination. Conclusions: It is possible to design a strategy combining short IVF insemination, early fertilization evaluation, and rescue ICSI on eggs that failed to fertilize after conventional IVF. 65 CORRELATION BETWEEN SEMINAL PLASMA SELENIUM CONCENTRATION AND MALE INFERTILITY A. Eidi1 , M. Eidi2 . 1 Islamic Azad University, Science and Research Branch, Department of Biology, Tehran, Iran, 2 Islamic Azad University, Varamin Branch, Department of Biology, Varamin, Iran Selenium is an essential trace nutrient for humans and animals. Selenium is required for normal testicular development and spermatogenesis. In the present study, correlations between seminal plasma glutathione peroxidase enzyme activity (as selenium status) and semen parameters are evaluated in 240 males. Semen analysis was performed according to World Health Organization guidelines. The 240 males were subdivided into 5 groups as normospermia, oligozospermia, asthenozospermia, azospermia and varicocele according to their spermiogrames. Seminal plasma glutathione peroxidase enzyme activity was determined by Kit (Randox, Germany). The result showed that glutathione peroxidase enzyme activity is higher in normospermic than oligozospermia, asthenozospermia, azospermia and varicocele groups. Also, there are significant and negative correlations between glutathione peroxidase enzyme

S27 activity and seminal plasma fructose concentration, white blood cell, tail defects of sperm, coiled tail sperms and short tail sperms. On the other hand, the present data showed that significant and positive correlations between vitality, sperm count, motility and normal morphology. So, the present study showed that measurement of glutathione peroxidase enzyme activity as selenium status could be a good marker for evaluation of male infertility. 66 COMPARISON OF PLASMA SEMINAL L-CARNITINE CONCENTRATIONS IN ASTHENOZOOSPERMIC, TERATOZOOSPERMIC, OLIGOZOOSPERMIC AND NECROZOOSPERMIC INFERTILE MEN M. Eidi1 , A. Eidi2 , O. Pouyan3 , P. Shahmohammadi1 , M. Bahar4 . 1 Department of Biology, Science Faculty, Varamin Branch, Islamic Azad University, Varamin, Iran, 2 Islamic Azad University, Science and Research Branch, Department of Biology, Tehran, Iran, 3 Laleh Hospital, Department of Urology, Tehran, Iran, 4 Department of Genetic, Medicine School, Tarbiat Modares University, Tehran, Iran The key role of L-carnitine in sperm metabolism is proposed by high level of L-carnitine in epididymis fluid. In present study, plasma seminal L-carnitine concentrations in asthenozoospermic, oligozoospermic, teratozoospermic and necrozoospermic infertile men are compared. Semen analysis was performed according to World Health Organization guidelines. Sperm parameters included liquefaction, pH, volume, sperm count, motility, viability and normal morphology. The results showed that plasma seminal L-carnitine concentration in asthenozoospermic specimens was lower than specimens which had high motility, significantly (p < 0.001). On the other hand, plasma seminal L-carnitine concentration in necrozoospermic specimens was lower than specimens which had high viability, significantly (p < 0.001). Also, plasma seminal L-carnitine concentration in teratozoospermic specimens was lower than specimens which had high normal morphology, significantly (p < 0.01). Plasma seminal L-carnitine concentration in oligozoospermic specimens was lower than specimens which had high sperm count, significantly (p < 0.001). In conclusions, plasma seminal L-carnitine concentration in infertile men with low quality semen parameters supplementation is lower than men with normal semen quality. So, L-carnitine treatment could affect on men infertility. 67 CIGARETTE SMOKING AND THE RISK OF MALE INFERTILITY M. Eidi1 , A. Eidi2 , P. Shahmohammadi1 . 1 Islamic Azad University, Varamin Branch, Department of Biology, Varamin, Iran, 2 Islamic Azad University, Science and Research Branch, Department of Biology, Tehran, Iran Cigarette smoke includes number of substances that have negative effects on male reproductive system and cause to male infertility. In this research, we investigated the effect of cigarette smoking on sperm parameters in fertile and infertile men (n = 200). Semen analysis was performed according to the World Health Organization guidelines to obtain volume, pH, vitality, sperm concentration, motility, morphology and seminal plasma fructose concentration (World Health Organization, 2000). Sperm concentration was determined with a Neubauer® counting chamber. Motility was expressed as the percentage of motile spermatozoa into 4 categories (immotile, flagella, slow and rapid). Morphology was determined according to the WHO criteria using the Papanicolaou staining procedure. At least 300 cells were examined at a final magnification of 1000×. Our research showed that sperm parameters quality in smoker men