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721 Discrepancies between allergen- and mite-content of dust samples (and its consequence for avoidance)
320
721
722
Abstracts
DISCREPANCIES BETWEEN ALLERGENAND MITECONTENT OF DUST SAMPLES (AND ITS CONSEQUENCE FOR AVOIDANCE). F.M.Kniest, Ph.D., E. Bischoff, Ph.D., J.E.M.H.vm ;w11 _ ., Malnz, tRc L. D and Utrecht, The Netherlands Mite (Ml counts, allergen (A) and quanine levels are used for evaluation of efficacy of avoidance measures. We compared their results using dust from M cultures (3), hay (1) and textile ObJects (23). Mat. and meth.: 27 samples were examined for A (Pl,Fl,PIl by RIA), M (flotation) and G (quant. by HPLC). Results: k levels: Cultures D.farinae (Pl:D,3; F1:15017; PII: 700). D.pteronys. (P1:260; FI:9; ?11:55!, D.microcer. (P1:91; F1:4382; PII:2751, other samples (ranges) Pl:D,l-127; F1:1,3-1540; PII:O.l-249. "1 counts: 2 samples were without Pyroglyphidae, 15 samples contained also other M, of which In 6 cases in higher numbers. Group i, group II, nor group it11 allergens showed any significant correlation (Soearman) with total M (P>O.Ol). G values showed a positive correlation (p
723
QUANTIFICATIONOFIPI~DDER
724
Netherlands. Extracts of 30 mattress and carpet dust samples were analyzed for concentrations of the major allergen Der p I of Dermatophaqoides pteronyssinus with two assays using different monoclonal antibodies in order to compare the concordance of results. Furthermore, Der f I and Der p II were determined. There correlation between the was a good values for Der p I obtained in the two different labs (r=0.87, p
OF nITE
EXPqSUBE VITY. B,
hND
rt . S. BA,-..WABerlin, Germany. order to study the therapeutic efficacy of different indoor allergen elimination procedures we studied 24 asthmatic children with hypersensitlvity (skin test, specific serum 1gE:) living in houses to D. pteronyssinus, with Der p I concentrations of >2pq/q Mattresses and carpets mattress dust. e.ither were treated for 12 months with benzyl benzoate (n=8) or placebo (n=B) in a double blind fashion. In a were: third (n=6) carpets group treated with 3% tannic ac:d, while comforts and mattresses were pillows, covered with encasings. Der p I and Der f I concentrations (sandwich type in mattress and carpet dust ELISA) as well as bronchial hypersamples, for reactivity PCZOFEVi (BHR, were followed prospechistamine) tively (before treatment, dt months treat4, 8, and 12). Benzyl benzoate ment resulted in a slight decrease in concentrations allergen mattress without any change in BHR. Only the tannic acid/encasing regime led tc a significant decrease in mite allergen We conclude that an and BHR (p
Wahn, In
WE EFFECTS OF FIIR CDNDITIONlNG ON THE PrpW WCE OF -WJEET ?tlTE Al 1 EROENG T. J. Lintner. Ph.D.. C. Schou. Ph.D.. L. M. Mulfinaer. Ph.D.. fl. W. Guralnick.
M.S.. K. A. Brame. B.S. Spring Pennsvtvania and Coffenhaoen. Extracts
were
samples
prepare;
collected
1989 and September, across
the
and
f I,
Der
sured
using
Air
in an
the enzyme
extracts were immunoassay.
conditioning
(P
both There
(CIC)
decreased p I and
360
the
Der
f
mea--
significantly concentration I allergens. (P
of
was a significant action between AC and time with the largest the year,
(months) difference
observed cooling
May
tember
during .
reached
a
Der
peak
associated
f
cooling
I
gradually
about
with group
Dermatophagoides rily
inter-
the summer months, from
season, In homes in
July,
with
dust
September,
1990 from 271 homes allergens, Der p I
Mite
USA.
Mills, Denmark.
f;am
between
to
the 5ep-
no AC, the I a ller-gens which
of
total
prima-
was
a rise in Der increased during was responsible
P I. the
season and for slow decline of Dermatophagoldes group I allergens through the end of the cooling season. Humidity within homes is an Important factor in determining the density of Dermataphagoides mite populations. The the
use
of
midity mite production.
.
AC possibly decreases and temperature enough populations and subsequent
indoor to
hureduce allergen
721
722
Abstracts
DISCREPANCIES BETWEEN ALLERGENAND MITECONTENT OF DUST SAMPLES (AND ITS CONSEQUENCE FOR AVOIDANCE). F.M.Kniest, Ph.D., E. Bischoff, Ph.D., J.E.M.H.vm ;w11 _ ., Malnz, tRc L. D and Utrecht, The Netherlands Mite (Ml counts, allergen (A) and quanine levels are used for evaluation of efficacy of avoidance measures. We compared their results using dust from M cultures (3), hay (1) and textile ObJects (23). Mat. and meth.: 27 samples were examined for A (Pl,Fl,PIl by RIA), M (flotation) and G (quant. by HPLC). Results: k levels: Cultures D.farinae (Pl:D,3; F1:15017; PII: 700). D.pteronys. (P1:260; FI:9; ?11:55!, D.microcer. (P1:91; F1:4382; PII:2751, other samples (ranges) Pl:D,l-127; F1:1,3-1540; PII:O.l-249. "1 counts: 2 samples were without Pyroglyphidae, 15 samples contained also other M, of which In 6 cases in higher numbers. Group i, group II, nor group it11 allergens showed any significant correlation (Soearman) with total M (P>O.Ol). G values showed a positive correlation (p
723
QUANTIFICATIONOFIPI~DDER
724
Netherlands. Extracts of 30 mattress and carpet dust samples were analyzed for concentrations of the major allergen Der p I of Dermatophaqoides pteronyssinus with two assays using different monoclonal antibodies in order to compare the concordance of results. Furthermore, Der f I and Der p II were determined. There correlation between the was a good values for Der p I obtained in the two different labs (r=0.87, p
OF nITE
EXPqSUBE VITY. B,
hND
rt . S. BA,-..WABerlin, Germany. order to study the therapeutic efficacy of different indoor allergen elimination procedures we studied 24 asthmatic children with hypersensitlvity (skin test, specific serum 1gE:) living in houses to D. pteronyssinus, with Der p I concentrations of >2pq/q Mattresses and carpets mattress dust. e.ither were treated for 12 months with benzyl benzoate (n=8) or placebo (n=B) in a double blind fashion. In a were: third (n=6) carpets group treated with 3% tannic ac:d, while comforts and mattresses were pillows, covered with encasings. Der p I and Der f I concentrations (sandwich type in mattress and carpet dust ELISA) as well as bronchial hypersamples, for reactivity PCZOFEVi (BHR, were followed prospechistamine) tively (before treatment, dt months treat4, 8, and 12). Benzyl benzoate ment resulted in a slight decrease in concentrations allergen mattress without any change in BHR. Only the tannic acid/encasing regime led tc a significant decrease in mite allergen We conclude that an and BHR (p
Wahn, In
WE EFFECTS OF FIIR CDNDITIONlNG ON THE PrpW WCE OF -WJEET ?tlTE Al 1 EROENG T. J. Lintner. Ph.D.. C. Schou. Ph.D.. L. M. Mulfinaer. Ph.D.. fl. W. Guralnick.
M.S.. K. A. Brame. B.S. Spring Pennsvtvania and Coffenhaoen. Extracts
were
samples
prepare;
collected
1989 and September, across
the
and
f I,
Der
sured
using
Air
in an
the enzyme
extracts were immunoassay.
conditioning
(P
both There
(CIC)
decreased p I and
360
the
Der
f
mea--
significantly concentration I allergens. (P
of
was a significant action between AC and time with the largest the year,
(months) difference
observed cooling
May
tember
during .
reached
a
Der
peak
associated
f
cooling
I
gradually
about
with group
Dermatophagoides rily
inter-
the summer months, from
season, In homes in
July,
with
dust
September,
1990 from 271 homes allergens, Der p I
Mite
USA.
Mills, Denmark.
f;am
between
to
the 5ep-
no AC, the I a ller-gens which
of
total
prima-
was
a rise in Der increased during was responsible
P I. the
season and for slow decline of Dermatophagoldes group I allergens through the end of the cooling season. Humidity within homes is an Important factor in determining the density of Dermataphagoides mite populations. The the
use
of
midity mite production.
.
AC possibly decreases and temperature enough populations and subsequent
indoor to
hureduce allergen