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748 PROSTATE CANCER RISK ASSOCIATION WITH SNP POLYMORPHIC VARIANTS OF CYP1B1 GENE IN THE BULGARIAN POPULATION
WKHDQDO\VLVRIWKHPDWFKHGVDPSOHVWKHVWURQJHVWGRZQUHJXODWLRQZDVREVHUYHG IRUKVDPL5ZLWKDPHGLDQH[SUHVVLRQOHYHORI1RVLJQLᚏFDQWDVVRFLDWLRQV EHWZHHQWKHGLᚎHUHQWLDOPL5H[SUHVVLRQDQGWXPRXUVWDJHRU*OHDVRQVFRUHZHUH IRXQG 7KH SUHYLRXVO\ GHWHUPLQHG XSUHJXODWLRQ RI WKH VHOHFWHG 3&DDVVRFLDWHG JHQHV VLJQLᚏFDQWO\ FRUUHODWHG ZLWK WKH GRZQUHJXODWLRQ RI WKHLU SUHGLFWHG PL5 UHJXODWRUVZLWK6SHDUPDQFRUUHODWLRQFRHᚑFLHQWVUDQJLQJIURPWR Conclusions: 2XUᚏQGLQJVLQGLFDWHWKDWVSHFLᚏFPL5DUHGHFUHDVHGLQ3&DDQG WKDWWKHLUH[SUHVVLRQOHYHOVFRUUHODWHZLWKWKHXSUHJXODWLRQRIWKHLUSXWDWLYHWDUJHW JHQHVWKDWDUHRYHUH[SUHVVHGLQ3&D%\WDUJHWLQJDQGFRQWUROOLQJWKHH[SUHVVLRQ RI3&DDVVRFLDWHGJHQHVPL5FRXOGFRQWULEXWHWRWKHRQFRJHQHVLVDQGSURJUHVVLRQ RI 3&D 7KH GDWD SUHVHQWHG KHUHLQ LQGLFDWH WKDW PL5 SRWHQWLDOO\ FRXOG LQᚐXHQFH WKHH[SUHVVLRQRI3&DDVVRFLDWHGJHQHV7KHVHUHVXOWVSURYLGHDEDVLVIRUIXUWKHU IXQFWLRQDOVWXGLHVRIPL5LQ3&D
748
PROSTATE CANCER RISK ASSOCIATION WITH SNP POLYMORPHIC VARIANTS OF CYP1B1 GENE IN THE BULGARIAN POPULATION
3RSRY(3.DFKDNRYD'0LWNRYD$.DQHYD59ODKRYD$'LNRY7 6ODYRY&.0LWHY9.UHPHQVN\, 1 Alexandrovska University Hospital, Medical University, Dept. of Urology, Sofia, Bulgaria, 2Molecular Medicine Center, Medical University, Sofia, Bulgaria, 3 Molecular Medicine Center, Medical University, Dept. of Chemistry and Biochemistry, Sofia, Bulgaria, 4Aleksandrovska University Hospital, Medical University, Dept. of Pathology, Sofia, Bulgaria, 5University Obs/Gyn Hospital Maichin Dom, Medical University, National Genetic Laboratory, Sofia, Bulgaria Introduction & Objectives: 6H[ KRUPRQHV SOD\ NH\ UROH LQ WKH JURZWK DQG GHYHORSPHQW RI SURVWDWH JODQG DQG DOVR DUH DVVRFLDWHG ZLWK GHYHORSPHQW RI SURVWDWHFDQFHU 3& 7KHLULQᚐXHQFHRQSURVWDWHFDUFLQRJHQHVLVLVPXOWLIDFWRULDO FRPSOH[ DQG VWLOO SRRUO\ XQGHUVWRRG 7KH\ DUH VLJQLᚏ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aterial & Methods: :HKDYH LQYHVWLJDWHG WKH DVVRFLDWLRQ ZLWK 3& ULVN RI IRXU SRO\PRUSKLVPV LQ H[RQV DQG RI &<3% LQ D FDVHFRQWURO VWXG\ RI 3& SDWLHQWVDQGFRQWUROLQGLYLGXDOVZLWKEHQLJQSURVWDWHK\SHUSODVLDDQGQRUPDO 36$OHYHO7KHSRO\PRUSKLVPVZHUHJHQRW\SHGE\GLUHFWVHTXHQFLQJRQ$%,3ULVP [O*HQHWLF$QDO\]HUDQGVFRUHGE\6HT6FDSHSURJUDPPH Results: 7KH VWURQJHVW DVVRFLDWLRQ ZLWK 3& ULVN ZDV GHPRQVWUDWHG E\ ' ' ZKHUHWKHSUHVHQFHRIHLWKHURQH 25 &, S RUWZR& DOOHOHV 25 &, S OHDGVWRIROGLQFUHDVHGULVN7KH &&JHQRW\SHRFFXUUHGLQKLJKHUIUHTXHQF\LQSDWLHQWV WKHQLQWKHFRQWUROV 6LPLODUO\ LQ WHUPV RI /9 WKH IUHTXHQW && JHQRW\SH DQG DPRQJ SDWLHQWV DQG FRQWUROV UHVSHFWLYHO\ ZDV DVVRFLDWHG ZLWK PRUH WKDQ IROG3&ULVN 25 &, S 7KHSRO\PRUSKLVPV16 UV DQG$ 6 UV*7 GLGQRWVKRZDQ\VLJQLᚏFDQWDVVRFLDWLRQ ZLWK3&ULVN Conclusions: )XQFWLRQDOLQYHVWLJDWLRQVVKRZWKDWVRPHFRPELQDWLRQVRI&<3% DOOHOHV FDQ VLJQLᚏFDQWO\ FKDQJH WKH NLQHWLF SURSHUWLHV RI WKH HQ]\PH 7KHUH DUH SRO\PRUSKLVPV OHDGLQJ WR DPLQR DFLG VXEVWLWXWLRQ OLNH UV 6HU $OD UV 9DO/HX UV 6HU$VQ 6RPHDXWKRUVGHPRQVWUDWHWKDW WKHFRPELQDWLRQRIWKHVH613VOHDGVWRDQHQ]\PHDFWLYLW\ZLWKWKHKLJKHVW.P DQGWKHORZHVW9PD[DQGWKDWVKRZVWKDWWKHVH613VLQᚐXHQFHWKHSURWHLQIROGLQJ 5HJDUGLQJWKHDQGURJHQPHWDEROLVPDWOHDVWVHYHUDOVWXGLHVKDYHGHPRQVWUDWHG WKHDVVRFLDWLRQEHWZHHQRQHRUPRUHJHQHWLFYDULDQWVRI&<3%DQGLQFUHDVHG 3&ULVN,WDSSHDUVWKDW&<3%SRO\PRUSKLVPV' 'DQG/9DUHDVVRFLDWHG ZLWKLQFUHDVHG3&ULVNLQWKH%XOJDULDQSRSXODWLRQ7KH&DOOHOHRIUVLVLQ OLQNDJHGLVHTXLOLEULXPZLWKWKH&DOOHOHRIUVZKLOHWKH7DOOHOHDSSHDUVLQ FRPELQDWLRQZLWKWKH*DOOHOH
749
PTEN GENE MUTATION AND PRIMARY PROSTATE CANCER PROGRESSION
6DOHP6$EHGL$0HKUVDL$5H]DHLGDQHVK03RXUPDQG* Urology Research Centre Medical Sciences, University of Tehran, Dept. of Urology, Tehran, Iran Introduction & Objectives: 37(1LVDSXWDWLYHWXPRXUVXSSUHVVRUJHQHORFDWHG RQTLQSURVWDWHFDQFHUVWKHVHFRQGPRVWFRPPRQFDXVHRIFDQFHUUHODWHG GHDWKLQPHQ7KH37(1PXWDWLRQUDWHLVQRWH[DFWO\GHWHUPLQHGHVSHFLDOO\LQ$VLDQ SHRSOH7KLVVWXG\VRXJKWWRIXUWKHUFODULI\WKHUROHRI37(1JHQHLQFRPELQDWLRQ ZLWK*OHDVRQVFRULQJIRUEHWWHUSUHGLFWLRQRISURJUHVVLRQDQGVXUYLYDORIFDQFHU DOVRWRLGHQWLI\37(1PXWDWLRQIUHTXHQF\DPRQJ,UDQLDQSDWLHQWV Material & Methods: )LIW\RQH FDVHV RI SULPDU\ SURVWDWH FDQFHU ZHUH VWXGLHG
Eur Urol Suppl 2010;9(2):242
DQG IROORZHG IRU DW OHDVW \HDUV 7KH PHWKRGV RI WLVVXH PLFURGLVVHFWLRQ DQG VLQJOHVWUDQGFRQIRUPDWLRQDOSRO\PRUSKLVP 3&566&3DQDO\VLV ZHUHXVHG7KH FOLQLFRSDWKRORJLFDOGDWDZHUHFROOHFWHG Results: )LIW\VHYHQ WZHQW\RQH DQG WZHQW\WZR SHUFHQWV RI WKH WXPRXUV ZHUH *OHDVRQ VFRUH *6 DQG ! UHVSHFWLYHO\ ,Q DGGLWLRQ WKH VWDJHV RI WKH WXPRXUVLQSULPDU\SURVWDWHFDQFHUVZHUH,,D ,,E ,,F DQG ,9 6L[RIᚏIW\RQH SULPDU\SURVWDWHFDQFHUVVKRZHGPXWDWLRQVLQ 37(1ZKLFKLQYROYHGH[RQVDQG7KHVWDJHVRIWKHWXPRXUVZLWKSRVLWLYH PXWDWLRQV ZHUH ,,D ,,E DQG ,9 2I WKHP DQG ZHUH*6 DQG!UHVSHFWLYHO\)LYHRIVL[SDWLHQWVGLHGDVDUHVXOWRIPHWDVWDVHV 3DWLHQWVZLWKDSRVLWLYHPXWDWLRQRI37(1KDGDVLJQLᚏFDQWO\JUHDWHU*6 3 ORZHUVXUYLYDOUDWH 3 KLJKHUWHQGHQF\WRPHWDVWDVLV 3 DQGKLJKHU SURVWDWHVSHFLᚏF DQWLJHQ 3 &R[ SURSRUWLRQDO KD]DUG PRGHO VKRZHG WKDW RQO\*6ZDVVLJQLᚏFDQWO\FRUUHODWHGZLWKPRUWDOLW\ 3 Conclusions: 7KH 37(1 PXWDWLRQ IUHTXHQF\ LQ ,UDQLDQ SDWLHQWV ZDV 3URVWDWH FDQFHU ZLWK SRVLWLYH 37(1 PXWDWLRQ KDV D ZRUVH SURJQRVLV JUHDWHU SURFOLYLW\WRPHWDVWDVHZLWKKLJKHUVWDJHDQGJUDGH+RZHYHUWKLVPXWDWLRQFDQQRW SUHGLFWWKHSURJQRVLVDQGWKH*6LVDPRUHSUHFLVHIDFWRU
750
GENE EXPRESSION ANALYSIS OF ETS TRANSCRIPTION FACTOR FAMILY GENES AND ANDROGEN RECEPTOR IN LASER CAPTURE MICRODISSECTED HUMAN PROSTATE CANCER TISSUE
/DGXUQHU5HQQDX00DVVRQHU36FK¦IHU*6HLIDUW&6XPPHUHU0 %UDQGVW¦WWHU$%DUWVFK*.ORFNHU+ 1 Innsbruck Medical University, Dept. of Urology, Innsbruck, Austria, 2Innsbruck Medical University, Dept. of Genetic Epidemiology, Innsbruck, Austria Introduction & Objectives: 5HFHQWO\DIUHTXHQWJHQHIXVLRQLQSURVWDWHFDQFHUV 3&D KDV EHHQ LGHQWLᚏHG LQYROYLQJ WKH ಬ XQWUDQVODWHG UHJLRQ RI WKH DQGURJHQ UHJXODWHG JHQH 703566 DQG WKH (76 WUDQVFULSWLRQ IDFWRU IDPLO\ JHQHV PRVW FRPPRQO\ (5* 703566(5* JHQH IXVLRQ UHVXOWV LQ (5* RYHUH[SUHVVLRQ ZKLFKPD\KDYHDGLUHFWFDXVDOUROHLQSURVWDWHWXPRXULJHQHVLVRUSURJUHVVLRQDQG KDVEHHQGHVFULEHGDVDSRVVLEOHPDUNHULQ3&D Material & Methods: :HLVRODWHGSURVWDWHFDQFHUFHOOVDQGPDWFKHGEHQLJQSURVWDWH HSLWKHOLDO FHOOV IURP KXPDQ SURVWDWH WLVVXH XVLQJ ODVHU FDSWXUH PLFURGLVVHFWLRQ /&0 DQGDQDO\]HGH[SUHVVLRQRI(76WUDQVFULSWLRQIDFWRUVDQGURJHQUHFHSWRUV $5 DQGFHOOXODUPDUNHUVE\5HDO7LPH3&53XULW\RI/&0VDPSOHLVRODWLRQZDV YDOLGDWHGE\DQDO\VLVRIH[SUHVVLRQSDWWHUQVRIFHOOVSHFLᚏFPDUNHUJHQHV Results: :HIRXQGDQLQYHUVHFRUUHODWLRQEHWZHHQWKHH[SUHVVLRQOHYHOVRI(5* DQG DQGURJHQ UHFHSWRU LQFOXGLQJ D VLJQLᚏFDQW S $5 GRZQ UHJXODWLRQ LQ (5* H[SUHVVLQJ WXPRXUV ([SUHVVLRQ RI (76 IDPLO\ PHPEHUV (76 DQG (/. FRUUHODWHGVLJQLᚏFDQWO\ S ZLWK(5*H[SUHVVLRQ Conclusions: $QGURJHQUHFHSWRULVVLJQLᚏFDQWO\GRZQUHJXODWHGLQ(5*H[SUHVVLQJ WXPRXUV VXJJHVWLQJ GHFUHDVHG DQGURJHQ GHSHQGHQFH LQ (5* RYHUH[SUHVVLQJ WXPRXUV
751
A NOVEL CANDIDATE GENE OF SUSCEPTIBILITY TO PROSTATE CANCER, FARNECYL-DIPHOSPHATE FARNECYLTRANSFERASE 1 (FDFT1) GENE: ASSOCIATION BETWEEN THE VARIANTS OF THE FDFT1 GENE AND THE PROSTATE CANCER RISK, AND THE EVALUATION OF THE FDFT1 PROTEIN FUNCTION TO THE PROSTATE CANCER CELLS
0DWVXL+)XNXPD<+DPDQR7.RLNH+6HNLQH<2KWDNH1 1DNDWD66X]XNL. 1 Gunma University Graduate School of Medicine, Dept. of Urology, Maebashi, Japan, 2Hidaka Hospital, Dept. of Urology, Takasaki, Japan, 3Ashikaga Red Cross Hospital, Dept. of Urology, Ashikaga, Japan Introduction & Objectives: :H SUHYLRXVO\ FRQGXFWHG D JHQRPHZLGH OLQNDJH DQDO\VLVLQQXFOHDU-DSDQHVHIDPLOLHVDᚎ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aterial & Methods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
748
PROSTATE CANCER RISK ASSOCIATION WITH SNP POLYMORPHIC VARIANTS OF CYP1B1 GENE IN THE BULGARIAN POPULATION
3RSRY(3.DFKDNRYD'0LWNRYD$.DQHYD59ODKRYD$'LNRY7 6ODYRY&.0LWHY9.UHPHQVN\, 1 Alexandrovska University Hospital, Medical University, Dept. of Urology, Sofia, Bulgaria, 2Molecular Medicine Center, Medical University, Sofia, Bulgaria, 3 Molecular Medicine Center, Medical University, Dept. of Chemistry and Biochemistry, Sofia, Bulgaria, 4Aleksandrovska University Hospital, Medical University, Dept. of Pathology, Sofia, Bulgaria, 5University Obs/Gyn Hospital Maichin Dom, Medical University, National Genetic Laboratory, Sofia, Bulgaria Introduction & Objectives: 6H[ KRUPRQHV SOD\ NH\ UROH LQ WKH JURZWK DQG GHYHORSPHQW RI SURVWDWH JODQG DQG DOVR DUH DVVRFLDWHG ZLWK GHYHORSPHQW RI SURVWDWHFDQFHU 3& 7KHLULQᚐXHQFHRQSURVWDWHFDUFLQRJHQHVLVLVPXOWLIDFWRULDO FRPSOH[ DQG VWLOO SRRUO\ XQGHUVWRRG 7KH\ DUH VLJQLᚏ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aterial & Methods: :HKDYH LQYHVWLJDWHG WKH DVVRFLDWLRQ ZLWK 3& ULVN RI IRXU SRO\PRUSKLVPV LQ H[RQV DQG RI &<3% LQ D FDVHFRQWURO VWXG\ RI 3& SDWLHQWVDQGFRQWUROLQGLYLGXDOVZLWKEHQLJQSURVWDWHK\SHUSODVLDDQGQRUPDO 36$OHYHO7KHSRO\PRUSKLVPVZHUHJHQRW\SHGE\GLUHFWVHTXHQFLQJRQ$%,3ULVP [O*HQHWLF$QDO\]HUDQGVFRUHGE\6HT6FDSHSURJUDPPH Results: 7KH VWURQJHVW DVVRFLDWLRQ ZLWK 3& ULVN ZDV GHPRQVWUDWHG E\ ' ' ZKHUHWKHSUHVHQFHRIHLWKHURQH 25 &, S RUWZR& DOOHOHV 25 &, S OHDGVWRIROGLQFUHDVHGULVN7KH &&JHQRW\SHRFFXUUHGLQKLJKHUIUHTXHQF\LQSDWLHQWV WKHQLQWKHFRQWUROV 6LPLODUO\ LQ WHUPV RI /9 WKH IUHTXHQW && JHQRW\SH DQG DPRQJ SDWLHQWV DQG FRQWUROV UHVSHFWLYHO\ ZDV DVVRFLDWHG ZLWK PRUH WKDQ IROG3&ULVN 25 &, S 7KHSRO\PRUSKLVPV16 UV DQG$ 6 UV*7 GLGQRWVKRZDQ\VLJQLᚏFDQWDVVRFLDWLRQ ZLWK3&ULVN Conclusions: )XQFWLRQDOLQYHVWLJDWLRQVVKRZWKDWVRPHFRPELQDWLRQVRI&<3% DOOHOHV FDQ VLJQLᚏFDQWO\ FKDQJH WKH NLQHWLF SURSHUWLHV RI WKH HQ]\PH 7KHUH DUH SRO\PRUSKLVPV OHDGLQJ WR DPLQR DFLG VXEVWLWXWLRQ OLNH UV 6HU $OD UV 9DO/HX UV 6HU$VQ 6RPHDXWKRUVGHPRQVWUDWHWKDW WKHFRPELQDWLRQRIWKHVH613VOHDGVWRDQHQ]\PHDFWLYLW\ZLWKWKHKLJKHVW.P DQGWKHORZHVW9PD[DQGWKDWVKRZVWKDWWKHVH613VLQᚐXHQFHWKHSURWHLQIROGLQJ 5HJDUGLQJWKHDQGURJHQPHWDEROLVPDWOHDVWVHYHUDOVWXGLHVKDYHGHPRQVWUDWHG WKHDVVRFLDWLRQEHWZHHQRQHRUPRUHJHQHWLFYDULDQWVRI&<3%DQGLQFUHDVHG 3&ULVN,WDSSHDUVWKDW&<3%SRO\PRUSKLVPV' 'DQG/9DUHDVVRFLDWHG ZLWKLQFUHDVHG3&ULVNLQWKH%XOJDULDQSRSXODWLRQ7KH&DOOHOHRIUVLVLQ OLQNDJHGLVHTXLOLEULXPZLWKWKH&DOOHOHRIUVZKLOHWKH7DOOHOHDSSHDUVLQ FRPELQDWLRQZLWKWKH*DOOHOH
749
PTEN GENE MUTATION AND PRIMARY PROSTATE CANCER PROGRESSION
6DOHP6$EHGL$0HKUVDL$5H]DHLGDQHVK03RXUPDQG* Urology Research Centre Medical Sciences, University of Tehran, Dept. of Urology, Tehran, Iran Introduction & Objectives: 37(1LVDSXWDWLYHWXPRXUVXSSUHVVRUJHQHORFDWHG RQTLQSURVWDWHFDQFHUVWKHVHFRQGPRVWFRPPRQFDXVHRIFDQFHUUHODWHG GHDWKLQPHQ7KH37(1PXWDWLRQUDWHLVQRWH[DFWO\GHWHUPLQHGHVSHFLDOO\LQ$VLDQ SHRSOH7KLVVWXG\VRXJKWWRIXUWKHUFODULI\WKHUROHRI37(1JHQHLQFRPELQDWLRQ ZLWK*OHDVRQVFRULQJIRUEHWWHUSUHGLFWLRQRISURJUHVVLRQDQGVXUYLYDORIFDQFHU DOVRWRLGHQWLI\37(1PXWDWLRQIUHTXHQF\DPRQJ,UDQLDQSDWLHQWV Material & Methods: )LIW\RQH FDVHV RI SULPDU\ SURVWDWH FDQFHU ZHUH VWXGLHG
Eur Urol Suppl 2010;9(2):242
DQG IROORZHG IRU DW OHDVW \HDUV 7KH PHWKRGV RI WLVVXH PLFURGLVVHFWLRQ DQG VLQJOHVWUDQGFRQIRUPDWLRQDOSRO\PRUSKLVP 3&566&3DQDO\VLV ZHUHXVHG7KH FOLQLFRSDWKRORJLFDOGDWDZHUHFROOHFWHG Results: )LIW\VHYHQ WZHQW\RQH DQG WZHQW\WZR SHUFHQWV RI WKH WXPRXUV ZHUH *OHDVRQ VFRUH *6 DQG ! UHVSHFWLYHO\ ,Q DGGLWLRQ WKH VWDJHV RI WKH WXPRXUVLQSULPDU\SURVWDWHFDQFHUVZHUH,,D ,,E ,,F DQG ,9 6L[RIᚏIW\RQH SULPDU\SURVWDWHFDQFHUVVKRZHGPXWDWLRQVLQ 37(1ZKLFKLQYROYHGH[RQVDQG7KHVWDJHVRIWKHWXPRXUVZLWKSRVLWLYH PXWDWLRQV ZHUH ,,D ,,E DQG ,9 2I WKHP DQG ZHUH*6 DQG!UHVSHFWLYHO\)LYHRIVL[SDWLHQWVGLHGDVDUHVXOWRIPHWDVWDVHV 3DWLHQWVZLWKDSRVLWLYHPXWDWLRQRI37(1KDGDVLJQLᚏFDQWO\JUHDWHU*6 3 ORZHUVXUYLYDOUDWH 3 KLJKHUWHQGHQF\WRPHWDVWDVLV 3 DQGKLJKHU SURVWDWHVSHFLᚏF DQWLJHQ 3 &R[ SURSRUWLRQDO KD]DUG PRGHO VKRZHG WKDW RQO\*6ZDVVLJQLᚏFDQWO\FRUUHODWHGZLWKPRUWDOLW\ 3 Conclusions: 7KH 37(1 PXWDWLRQ IUHTXHQF\ LQ ,UDQLDQ SDWLHQWV ZDV 3URVWDWH FDQFHU ZLWK SRVLWLYH 37(1 PXWDWLRQ KDV D ZRUVH SURJQRVLV JUHDWHU SURFOLYLW\WRPHWDVWDVHZLWKKLJKHUVWDJHDQGJUDGH+RZHYHUWKLVPXWDWLRQFDQQRW SUHGLFWWKHSURJQRVLVDQGWKH*6LVDPRUHSUHFLVHIDFWRU
750
GENE EXPRESSION ANALYSIS OF ETS TRANSCRIPTION FACTOR FAMILY GENES AND ANDROGEN RECEPTOR IN LASER CAPTURE MICRODISSECTED HUMAN PROSTATE CANCER TISSUE
/DGXUQHU5HQQDX00DVVRQHU36FK¦IHU*6HLIDUW&6XPPHUHU0 %UDQGVW¦WWHU$%DUWVFK*.ORFNHU+ 1 Innsbruck Medical University, Dept. of Urology, Innsbruck, Austria, 2Innsbruck Medical University, Dept. of Genetic Epidemiology, Innsbruck, Austria Introduction & Objectives: 5HFHQWO\DIUHTXHQWJHQHIXVLRQLQSURVWDWHFDQFHUV 3&D KDV EHHQ LGHQWLᚏHG LQYROYLQJ WKH ಬ XQWUDQVODWHG UHJLRQ RI WKH DQGURJHQ UHJXODWHG JHQH 703566 DQG WKH (76 WUDQVFULSWLRQ IDFWRU IDPLO\ JHQHV PRVW FRPPRQO\ (5* 703566(5* JHQH IXVLRQ UHVXOWV LQ (5* RYHUH[SUHVVLRQ ZKLFKPD\KDYHDGLUHFWFDXVDOUROHLQSURVWDWHWXPRXULJHQHVLVRUSURJUHVVLRQDQG KDVEHHQGHVFULEHGDVDSRVVLEOHPDUNHULQ3&D Material & Methods: :HLVRODWHGSURVWDWHFDQFHUFHOOVDQGPDWFKHGEHQLJQSURVWDWH HSLWKHOLDO FHOOV IURP KXPDQ SURVWDWH WLVVXH XVLQJ ODVHU FDSWXUH PLFURGLVVHFWLRQ /&0 DQGDQDO\]HGH[SUHVVLRQRI(76WUDQVFULSWLRQIDFWRUVDQGURJHQUHFHSWRUV $5 DQGFHOOXODUPDUNHUVE\5HDO7LPH3&53XULW\RI/&0VDPSOHLVRODWLRQZDV YDOLGDWHGE\DQDO\VLVRIH[SUHVVLRQSDWWHUQVRIFHOOVSHFLᚏFPDUNHUJHQHV Results: :HIRXQGDQLQYHUVHFRUUHODWLRQEHWZHHQWKHH[SUHVVLRQOHYHOVRI(5* DQG DQGURJHQ UHFHSWRU LQFOXGLQJ D VLJQLᚏFDQW S $5 GRZQ UHJXODWLRQ LQ (5* H[SUHVVLQJ WXPRXUV ([SUHVVLRQ RI (76 IDPLO\ PHPEHUV (76 DQG (/. FRUUHODWHGVLJQLᚏFDQWO\ S ZLWK(5*H[SUHVVLRQ Conclusions: $QGURJHQUHFHSWRULVVLJQLᚏFDQWO\GRZQUHJXODWHGLQ(5*H[SUHVVLQJ WXPRXUV VXJJHVWLQJ GHFUHDVHG DQGURJHQ GHSHQGHQFH LQ (5* RYHUH[SUHVVLQJ WXPRXUV
751
A NOVEL CANDIDATE GENE OF SUSCEPTIBILITY TO PROSTATE CANCER, FARNECYL-DIPHOSPHATE FARNECYLTRANSFERASE 1 (FDFT1) GENE: ASSOCIATION BETWEEN THE VARIANTS OF THE FDFT1 GENE AND THE PROSTATE CANCER RISK, AND THE EVALUATION OF THE FDFT1 PROTEIN FUNCTION TO THE PROSTATE CANCER CELLS
0DWVXL+)XNXPD<+DPDQR7.RLNH+6HNLQH<2KWDNH1 1DNDWD66X]XNL. 1 Gunma University Graduate School of Medicine, Dept. of Urology, Maebashi, Japan, 2Hidaka Hospital, Dept. of Urology, Takasaki, Japan, 3Ashikaga Red Cross Hospital, Dept. of Urology, Ashikaga, Japan Introduction & Objectives: :H SUHYLRXVO\ FRQGXFWHG D JHQRPHZLGH OLQNDJH DQDO\VLVLQQXFOHDU-DSDQHVHIDPLOLHVDᚎ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aterial & Methods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