- Email: [email protected]
76. Cryocapacitation of Alpaca (Vicugna pacos) sperm cell
364
Abstracts / Cryobiology 65 (2012) 339–366
Nadia Canorio Pariona received the Best Poster Award for this poster 76. Cryocapacitation of Alpaca (Vicugna pacos) sperm cell. Nadia Canorio Pariona, Fernando Paredes Arnedo, Martha Valdivia Cuya, Physiology of Animal Reproduction Laboratory, Universidad Nacional Mayor de San Marcos, Peru The process of sperm cryopreservation is widely used in the programs of assisted reproduction and genetic improvement of many species. One of the features in the cryopreservation process is a disturbance at the plasma membrane which leads to a premature acrosomal reaction and phosphorylation in tyrosine residues of many proteins, as well as declining in the motility, reducing the lifetime of the sperm cell. All these changes are the result of a special type of process called ‘‘cryocapacitation”, but the possible cause for this phenomenon has not been determined yet. The study of the state of phosphorylated proteins on tyrosine residues gives us information about the level of capacitation that affects the sperm cells and is used in several studies to determine the cryocapacitation. The alpaca is a domestic species of economic importance in our country. There are many techniques for managing this species in order to carry out a genetic improvement program. The aim of this study was to evaluate a possible state of cryocapacitation in cryopreserved sperm cells of alpaca; we evaluated the motility, viability, and plasma membrane integrity before and after the freezing/thawing process; the pattern of proteins phosphorylated in tyrosine residues and the ability of binding to the zona pellucida of these frozen/thawed sperm. We compared our results with those from in vitro capacitated sperm cells. The results indicate that cryopreservation directly affects motility, viability and plasma membrane because the values are always reduced in thawed sperm cells, showing that both in vitro capacitated and cryopreserved sperm offered the same pattern of proteins phosphorylated in tyrosine residues and it was observed that the number of spermatozoa bound to the zona pellucida in the case of in vitro capacitated spermatozoa was greater than that observed in cryopreserved spermatozoa, our data also confirms the ability to bind to the zona pellucida of cryopreserved sperm. Considering the combined results of all experiments, we could conclude that the cryopreserved spermatozoa of alpaca must be undergoing a process of cryocapacitation, induced by the cryopreservation process itself. Conflict of interest: None declared. Source of funding: None declared. E-mail address: [email protected] (N. Canorio Pariona) http://dx.doi.org/10.1016/j.cryobiol.2012.07.077
List of authors of abstracts Abazari, Alireza Acker, Jason P. Acosta, M. Adams, Serean L Adesida, Adetola B. Agca, Yuksel Albarello, N. Alberio, Ricardo Almizraq, Ruqayyah Alonso, Telma S. Alvarez, G. Anil, S. Antollini, Silvia S. Anufriev, A.I. Arias, Gustavo Aris, Mariana Arriaga, Juan Martín Baer, A. Balaban, Cecilia L. Ballesteros, Daniel Bar Dolev, Maya Bargelloni, L. Barrio, María Marcela Bellas, J. Bellonio, Débora Benson, James D. Berardi, F. Bernaski, Mario Blanco, Paula A. Blomsma, Sylvia A. Bolyukh, Vladimir F.
17 34, 7 19, 17 70 21 68 34 61 11 65 61 58 1 66 66 8 35, 49 31, 15 66 64 63 20 52, 1 66 36 5
69 64
55, 56 48
54
Bonavigna, Rafael Bonini, Ida C. Bos-Mikich, Adriana Bottai, Hebe Bouix, Marielle Braslavsky, Ido Browning, Gabriela Recagno Buera, María del Pilar Burgos, Rafael Buschiazzo, Jorgelina Bysykatova, I.P. Cabrita, E. Califano, Alicia Canorio Pariona, Nadia Cantelmo, L. Carnevale, Matías E. Carrillo, Alejandra Carro, María de las Mercedes Cartón-García, F. Celik, Yeliz Cenard, Stéphanie Cetica, P. Cheng, Jingjiang (Robin) Chernetsov, Oleg Chernyavskyy, V.F. Chirife, J. Cincotti, Alberto Clausen, A.M. Cochofel, J. Comizzoli, Pierre Contreras, Carlos Cordeiro, L.S. Córdova, Alex Courteau, Lynn A. Crowe, John H. Dalvit, G. Davidson, B. Davies, Peter L. de Albulquerque, Daniele Menezes de Godoy, Leandro Cesar de Graaf, Inge A.M. de Meester, L. de Muylder, P de Oliveira, Diego de Paz, Leonardo Juan de Resende, Emiko Kawakami Degtaryov, A.G. Digilio, A. Do, Hackwon Drori, Ran Duzelman, Valeria J. Egorov, N.N. Elliott, Gloria D. Elliott, Janet A.W. Eroglu, A. Faccio, R. Fadda, Sarah Fahy, Gregory M. Falcão, E Faltus, Miloš Ferreira, A.L. Filippova, G.V. Fleck, R.A. Fonseca, Fernanda Forbes, J. Fraser Fornari, Darci Carlos Fry, Allyson K. Fukui, Kuniaki Fuller, B. Gagliardi, R.F. Gale Samantha L. Galo, Juliana Minardi Galvagno, Miguel Garcia, R.O Gautier, J. Germogenov, N.I. Ghorbal, Sarrah Gier, Martin
63 61 18 37, 50 31, 1 24, 40 61, 32, 15, 25 76 73 55, 40 68 75 31 28, 11 47 5 43 44, 46 71 73 38 40 21 40 6 3 11 33 31 67 67 36 30 33 67 62 67 41, 71 57 31 66 32, 38 17, 8 7 46 36 23 26 23 27 14 28, 17 67 60 74 33 73 19 67 39 23, 28, 32, 50 47
56 48 39, 66 68 59 16, 75
56
50
45
59
59 47, 51
50
72, 73 50 41, 59
Abstracts / Cryobiology 65 (2012) 339–366 Godoy, Leandro Cesar González-Benito, Maria Elena Goya, Alejandra B. Goya, Rodolfo G. Graf, Daniel A. Graiver, Natalia Graves-Herring, Jennifer E. Groothuis, Geny M.M. Guan, Na Guibert, Edgardo E. Gurusamy, K Gutnisky, C. Healy, L Herráez, M.P. Higgins, Adam Z. Hirai, Dai Holovati, Jelena L. Holt, Andrew Hovanyecz, P. Isaev, A.P. Ivanova, T.I. Jamme, F. Jan, R.V. Jimylev, I.F. Jomaa, A Jomha, Nadr M. Katkov, Igor I. Kerschengoltz, B. M. Khlebnyy, E.S. Kim, Eunjung Kim, Hak Jun Kim, Suhee Kitamura, T. Klyuchnikov, Evgeny Korol, Ana M. Kotková, Renata Kröger, Nikolaus Lambardi, Maurizio Laouar, Leila Law, Garson K. Layman, L.C. Lee, Jun Hyuck Lee, Sung Gu Leite, R. Lieben, Pascale Lusianti, Ratih E. Machado, C.B. Maghdoori, Babak Mamprin, María E. Mansur, E. Marín, María T. Martínez-Páramo, S Martínez-Pastor, F. Martucci, P. Mazur, Peter Mazzobre, María Florencia McGann, Locksley E. McGowan, Lindsay, T. Mediavilla, M.G. Méjico, G. Menarim, Bruno Mercado, Leonardo Merhy, T.S.M. Mikhalyova, L.G. Milan, M Mizrahy, Ortal Molina-Garcia, Antonio Diego Mombru, A. Monterubbianesi, G. Mordoh, José Mordosova, N.I. Morin, Pier Jr. Morrish, Jonathan R. Motomura, V. Niino, Takao Nikiphorov, O.I. Okhlopkov, I.M. Okoneshnikova, M.V.
18 26 63 63 62 25 38 36 36 35, 33 11 14 16, 20, 74 34 69 37 42, 42 28 29 29 33 17 5 29, 27, 57 57 70 22 13 62 26 13 12 17 17 8 57 57 15 50 20 14 17 52, 23, 62 15 75 7 2 39, 17, 19 52, 35 40 1 72 42 15 48 26, 7 71 66 58 6 19 22 74 43 58 42
37, 55, 56, 62
75 60
58
43 29, 43
53, 54, 55 72, 73
66 51 53, 54
49
Olague, E. Olivieri, Aldo Oresti, Gerardo Martín Ott, Helena Pacchiarini, T. Pacheco, G Panis, B. Paredes Arnedo, Fernando Paredes, E. Parkes, H. Pascucci, Franco Passot, Stéphanie Peine, Sven Pellegrino, J.M. Pérez Campos, H. Pettinelli, J.A. Pimenta, M. Pizarro, D. Popov, A.A. Pranke, Patricia Prasad, Vinay Prokopiev, I.A. Pshennikov, A.E. Pucci Molineris, M. Putman, A. Ramírez, Alfredo Ratto, Marcelo Reardon, Anthony J. Reed, Barbara M. Remigailo, P.A. Ribeiro, Ricardo Pereira Riesco, M.F. Rios, Glenda Rivero, M.V. Robles, V. Rodríguez, Joaquin V. Rodríguez-Gil, Joan Enric Romero, M. Rosano, Germán L. Rosso, Osvaldo A. Ross-Rodriguez, Lisa U. Rowe, Arthur W. Saito, A. Saldias, M. Sanchez, G. Sansinena, M. Santagapita, Patricio Román Santos, M.V. Sarasquete, C Scandizzi, A. Sedalishchev, V.O. Sekov, A.N. Shein, A.A. Sigot, V. Simões-Gurgel, C. Sleptsov, S.M. Smith, J.F Soares, B.O. Solomonov, K.S. Solomonov, N.G. Solomonova, O.N. Sputtek, Andreas Storey, Kenneth B. Streit Jr., Danilo P. Strobel, Pablo Suescun, L. Sumida, S. Szurek, E. Tapia, Ivana, J. Taylor, J. Tchir, Jayme D.R. Teixeira, Aline Schneider Tervit, H. Robin Tiribelli, C. Ulloa, Omar Urra, Javier Valdivia Cuya, Martha Valenzuela, Pamela
365 16 69 68 24 16 23, 30 76 64 33 55 28, 13 37 7 73 73 52, 42 10 47 27 59 53 30 40 40 51 4 29 67 75 68 71 75 35, 40 7 9 62 47 13 22 7 7 44, 24, 44, 15, 52, 58 32 27 37 21 32, 19 73 41, 41, 58 13, 6 18, 40 7 22 8 66 19 34 26, 19 52 40 40 76 40
72
50
54
37, 52, 54, 55, 56, 62
45 39 45 16 54
59
58 42, 43, 58, 59 47 67
49
366 Van Vorst, Matt Vanvlasselaer, E. Vasilyeva, V.K. Vega, Julio Vianna, M. G Vladimirtseva, M.V. Walters, Christina Watts, E. Wildt, David E.
Abstracts / Cryobiology 65 (2012) 339–366 38 30 42 66 23, 72 32, 59 49 19 38
Yakhnenko, Ilya Yamamoto, Shin-ichi Young, L Zámecˇník, Jirˇí Zampolla, Tiziana Zaritzky, Noemí Zhang, Tiantian Zhou, Xianpei Zhurova, Mariia
5 74 14 26 18, 65 25, 44, 45 18, 65 17 69
Abstracts / Cryobiology 65 (2012) 339–366
Nadia Canorio Pariona received the Best Poster Award for this poster 76. Cryocapacitation of Alpaca (Vicugna pacos) sperm cell. Nadia Canorio Pariona, Fernando Paredes Arnedo, Martha Valdivia Cuya, Physiology of Animal Reproduction Laboratory, Universidad Nacional Mayor de San Marcos, Peru The process of sperm cryopreservation is widely used in the programs of assisted reproduction and genetic improvement of many species. One of the features in the cryopreservation process is a disturbance at the plasma membrane which leads to a premature acrosomal reaction and phosphorylation in tyrosine residues of many proteins, as well as declining in the motility, reducing the lifetime of the sperm cell. All these changes are the result of a special type of process called ‘‘cryocapacitation”, but the possible cause for this phenomenon has not been determined yet. The study of the state of phosphorylated proteins on tyrosine residues gives us information about the level of capacitation that affects the sperm cells and is used in several studies to determine the cryocapacitation. The alpaca is a domestic species of economic importance in our country. There are many techniques for managing this species in order to carry out a genetic improvement program. The aim of this study was to evaluate a possible state of cryocapacitation in cryopreserved sperm cells of alpaca; we evaluated the motility, viability, and plasma membrane integrity before and after the freezing/thawing process; the pattern of proteins phosphorylated in tyrosine residues and the ability of binding to the zona pellucida of these frozen/thawed sperm. We compared our results with those from in vitro capacitated sperm cells. The results indicate that cryopreservation directly affects motility, viability and plasma membrane because the values are always reduced in thawed sperm cells, showing that both in vitro capacitated and cryopreserved sperm offered the same pattern of proteins phosphorylated in tyrosine residues and it was observed that the number of spermatozoa bound to the zona pellucida in the case of in vitro capacitated spermatozoa was greater than that observed in cryopreserved spermatozoa, our data also confirms the ability to bind to the zona pellucida of cryopreserved sperm. Considering the combined results of all experiments, we could conclude that the cryopreserved spermatozoa of alpaca must be undergoing a process of cryocapacitation, induced by the cryopreservation process itself. Conflict of interest: None declared. Source of funding: None declared. E-mail address: [email protected] (N. Canorio Pariona) http://dx.doi.org/10.1016/j.cryobiol.2012.07.077
List of authors of abstracts Abazari, Alireza Acker, Jason P. Acosta, M. Adams, Serean L Adesida, Adetola B. Agca, Yuksel Albarello, N. Alberio, Ricardo Almizraq, Ruqayyah Alonso, Telma S. Alvarez, G. Anil, S. Antollini, Silvia S. Anufriev, A.I. Arias, Gustavo Aris, Mariana Arriaga, Juan Martín Baer, A. Balaban, Cecilia L. Ballesteros, Daniel Bar Dolev, Maya Bargelloni, L. Barrio, María Marcela Bellas, J. Bellonio, Débora Benson, James D. Berardi, F. Bernaski, Mario Blanco, Paula A. Blomsma, Sylvia A. Bolyukh, Vladimir F.
17 34, 7 19, 17 70 21 68 34 61 11 65 61 58 1 66 66 8 35, 49 31, 15 66 64 63 20 52, 1 66 36 5
69 64
55, 56 48
54
Bonavigna, Rafael Bonini, Ida C. Bos-Mikich, Adriana Bottai, Hebe Bouix, Marielle Braslavsky, Ido Browning, Gabriela Recagno Buera, María del Pilar Burgos, Rafael Buschiazzo, Jorgelina Bysykatova, I.P. Cabrita, E. Califano, Alicia Canorio Pariona, Nadia Cantelmo, L. Carnevale, Matías E. Carrillo, Alejandra Carro, María de las Mercedes Cartón-García, F. Celik, Yeliz Cenard, Stéphanie Cetica, P. Cheng, Jingjiang (Robin) Chernetsov, Oleg Chernyavskyy, V.F. Chirife, J. Cincotti, Alberto Clausen, A.M. Cochofel, J. Comizzoli, Pierre Contreras, Carlos Cordeiro, L.S. Córdova, Alex Courteau, Lynn A. Crowe, John H. Dalvit, G. Davidson, B. Davies, Peter L. de Albulquerque, Daniele Menezes de Godoy, Leandro Cesar de Graaf, Inge A.M. de Meester, L. de Muylder, P de Oliveira, Diego de Paz, Leonardo Juan de Resende, Emiko Kawakami Degtaryov, A.G. Digilio, A. Do, Hackwon Drori, Ran Duzelman, Valeria J. Egorov, N.N. Elliott, Gloria D. Elliott, Janet A.W. Eroglu, A. Faccio, R. Fadda, Sarah Fahy, Gregory M. Falcão, E Faltus, Miloš Ferreira, A.L. Filippova, G.V. Fleck, R.A. Fonseca, Fernanda Forbes, J. Fraser Fornari, Darci Carlos Fry, Allyson K. Fukui, Kuniaki Fuller, B. Gagliardi, R.F. Gale Samantha L. Galo, Juliana Minardi Galvagno, Miguel Garcia, R.O Gautier, J. Germogenov, N.I. Ghorbal, Sarrah Gier, Martin
63 61 18 37, 50 31, 1 24, 40 61, 32, 15, 25 76 73 55, 40 68 75 31 28, 11 47 5 43 44, 46 71 73 38 40 21 40 6 3 11 33 31 67 67 36 30 33 67 62 67 41, 71 57 31 66 32, 38 17, 8 7 46 36 23 26 23 27 14 28, 17 67 60 74 33 73 19 67 39 23, 28, 32, 50 47
56 48 39, 66 68 59 16, 75
56
50
45
59
59 47, 51
50
72, 73 50 41, 59
Abstracts / Cryobiology 65 (2012) 339–366 Godoy, Leandro Cesar González-Benito, Maria Elena Goya, Alejandra B. Goya, Rodolfo G. Graf, Daniel A. Graiver, Natalia Graves-Herring, Jennifer E. Groothuis, Geny M.M. Guan, Na Guibert, Edgardo E. Gurusamy, K Gutnisky, C. Healy, L Herráez, M.P. Higgins, Adam Z. Hirai, Dai Holovati, Jelena L. Holt, Andrew Hovanyecz, P. Isaev, A.P. Ivanova, T.I. Jamme, F. Jan, R.V. Jimylev, I.F. Jomaa, A Jomha, Nadr M. Katkov, Igor I. Kerschengoltz, B. M. Khlebnyy, E.S. Kim, Eunjung Kim, Hak Jun Kim, Suhee Kitamura, T. Klyuchnikov, Evgeny Korol, Ana M. Kotková, Renata Kröger, Nikolaus Lambardi, Maurizio Laouar, Leila Law, Garson K. Layman, L.C. Lee, Jun Hyuck Lee, Sung Gu Leite, R. Lieben, Pascale Lusianti, Ratih E. Machado, C.B. Maghdoori, Babak Mamprin, María E. Mansur, E. Marín, María T. Martínez-Páramo, S Martínez-Pastor, F. Martucci, P. Mazur, Peter Mazzobre, María Florencia McGann, Locksley E. McGowan, Lindsay, T. Mediavilla, M.G. Méjico, G. Menarim, Bruno Mercado, Leonardo Merhy, T.S.M. Mikhalyova, L.G. Milan, M Mizrahy, Ortal Molina-Garcia, Antonio Diego Mombru, A. Monterubbianesi, G. Mordoh, José Mordosova, N.I. Morin, Pier Jr. Morrish, Jonathan R. Motomura, V. Niino, Takao Nikiphorov, O.I. Okhlopkov, I.M. Okoneshnikova, M.V.
18 26 63 63 62 25 38 36 36 35, 33 11 14 16, 20, 74 34 69 37 42, 42 28 29 29 33 17 5 29, 27, 57 57 70 22 13 62 26 13 12 17 17 8 57 57 15 50 20 14 17 52, 23, 62 15 75 7 2 39, 17, 19 52, 35 40 1 72 42 15 48 26, 7 71 66 58 6 19 22 74 43 58 42
37, 55, 56, 62
75 60
58
43 29, 43
53, 54, 55 72, 73
66 51 53, 54
49
Olague, E. Olivieri, Aldo Oresti, Gerardo Martín Ott, Helena Pacchiarini, T. Pacheco, G Panis, B. Paredes Arnedo, Fernando Paredes, E. Parkes, H. Pascucci, Franco Passot, Stéphanie Peine, Sven Pellegrino, J.M. Pérez Campos, H. Pettinelli, J.A. Pimenta, M. Pizarro, D. Popov, A.A. Pranke, Patricia Prasad, Vinay Prokopiev, I.A. Pshennikov, A.E. Pucci Molineris, M. Putman, A. Ramírez, Alfredo Ratto, Marcelo Reardon, Anthony J. Reed, Barbara M. Remigailo, P.A. Ribeiro, Ricardo Pereira Riesco, M.F. Rios, Glenda Rivero, M.V. Robles, V. Rodríguez, Joaquin V. Rodríguez-Gil, Joan Enric Romero, M. Rosano, Germán L. Rosso, Osvaldo A. Ross-Rodriguez, Lisa U. Rowe, Arthur W. Saito, A. Saldias, M. Sanchez, G. Sansinena, M. Santagapita, Patricio Román Santos, M.V. Sarasquete, C Scandizzi, A. Sedalishchev, V.O. Sekov, A.N. Shein, A.A. Sigot, V. Simões-Gurgel, C. Sleptsov, S.M. Smith, J.F Soares, B.O. Solomonov, K.S. Solomonov, N.G. Solomonova, O.N. Sputtek, Andreas Storey, Kenneth B. Streit Jr., Danilo P. Strobel, Pablo Suescun, L. Sumida, S. Szurek, E. Tapia, Ivana, J. Taylor, J. Tchir, Jayme D.R. Teixeira, Aline Schneider Tervit, H. Robin Tiribelli, C. Ulloa, Omar Urra, Javier Valdivia Cuya, Martha Valenzuela, Pamela
365 16 69 68 24 16 23, 30 76 64 33 55 28, 13 37 7 73 73 52, 42 10 47 27 59 53 30 40 40 51 4 29 67 75 68 71 75 35, 40 7 9 62 47 13 22 7 7 44, 24, 44, 15, 52, 58 32 27 37 21 32, 19 73 41, 41, 58 13, 6 18, 40 7 22 8 66 19 34 26, 19 52 40 40 76 40
72
50
54
37, 52, 54, 55, 56, 62
45 39 45 16 54
59
58 42, 43, 58, 59 47 67
49
366 Van Vorst, Matt Vanvlasselaer, E. Vasilyeva, V.K. Vega, Julio Vianna, M. G Vladimirtseva, M.V. Walters, Christina Watts, E. Wildt, David E.
Abstracts / Cryobiology 65 (2012) 339–366 38 30 42 66 23, 72 32, 59 49 19 38
Yakhnenko, Ilya Yamamoto, Shin-ichi Young, L Zámecˇník, Jirˇí Zampolla, Tiziana Zaritzky, Noemí Zhang, Tiantian Zhou, Xianpei Zhurova, Mariia
5 74 14 26 18, 65 25, 44, 45 18, 65 17 69