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8504663 Method of detecting nucleic acid sequences
PATENT ABSTRACTS DNA fraction having a structural gene which codes human interleukin 2 peptide are linked to each other. This novel DNA makes it possible to produce a polypeptide in which the two peptides arelinked to each other by transforming host microorganism with the DNA and culturing the transformed microorganism.
8504663 METHOD OF DETECTING NUCLEIC ACID SEQUENCES James L HARTLEY. Mark S BERNINGER assigned to LIFE TECHNOLOGIES INC; A process for detecting specific nucleotide sequences, called targets, in which a special DNA probe molecule, called a probe-vector, is capable of transforming bacteria if and only if it is held in a circular configuration by base pairing to a target nucleic acid, said transformation resulting in the detection of a phenotype specified by the probe-vector, said detection establishing the presence, absence, or quantity of the target; and a probe-vector molecule for performing the process.
8504673
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Mary Louise SKOTN1CKI. Antek Henryk SKOTNICKI, Adrian John GIBBS, 54 A'Beckett Street, Watson, ACT 2600, Australia assigned to THE A U S T R A L I A N NATIONAL UNIVERSITY A method of direct insertion of double-stranded RNA into a cloning vector consisting of doublestranded DNA, the method comprising: a) isolating the dsRNA from a source of interest: b) if required, decapping the dsRNA: and c) directly ligating the dsRna to the dsDNA of the appropriate cloning vector. This method allows dsRNA to be cloned directly, thus eliminating the traditional step of first making a complementary DNA copy of the RNA molecule. Using this method, it is possible to construct probes for the detection of virus diseases in plants and animals. The method can also be used to produce new plant strains which are resistant to a particular virus, or to provide immunity to animals to a specific virus.
8504899 METHODS AND VECTORS FOR TRANSFORMATION OF PLANT CELLS David H GELFAND, Kenneth A BARTON assigned to CETUS CORPORATION: AGRACETUS
NOVEL DNA AND ITS USE Masaharu SENOO, Haruo ONDA, Koichi IGARASHL D73-106, 18 Tsukumodai 5chome. Suita-shi, Osaka 565, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD: A novel DNA is prepared in which a DNA fraction having a structural gene which code a peptide containing an antibody recognition site and a DNA fraction having a structural gene which code human interleukin 2 peptide are linked to each other. This novel DNA makes it possible to produce a polypeptide in which the two peptides are linked to each other by transforming host microorganism with the DNA and culturing the transformed microorganism.
Vectors and methods suitable for both direct and Agrobacterium)-mediated transformation of plants. The vectors comprise easily manipulated expression cassettes and optionally contain border sequences associated with Agrobacterium) plasmid DNA transfer. By using an intermediate carrier vector which contains the requisite expression control sequences in accessible form, a wide variety of single and multiple cassette vectors can be constructed.
8505122 ROTAVIRUS
8504898 AN IMPROVED METHOD OF CLONING DOUBLE-STRANDED RNA, AND ITS USE IN THE PRODUCTION OF VIRAL GENE CLONES
lan Hamilton HOLMES, Michael Leigh DYALL-SMITH. 13 Avenue Athol, Canterbury. VIC 3126, Australia assigned to THE UNIVERSITY OF MELBOURNE A material being a dsRNA gene segment coding Ibr the major outer capsid glycoprotein of a rolavirus.
8504663 METHOD OF DETECTING NUCLEIC ACID SEQUENCES James L HARTLEY. Mark S BERNINGER assigned to LIFE TECHNOLOGIES INC; A process for detecting specific nucleotide sequences, called targets, in which a special DNA probe molecule, called a probe-vector, is capable of transforming bacteria if and only if it is held in a circular configuration by base pairing to a target nucleic acid, said transformation resulting in the detection of a phenotype specified by the probe-vector, said detection establishing the presence, absence, or quantity of the target; and a probe-vector molecule for performing the process.
8504673
145
Mary Louise SKOTN1CKI. Antek Henryk SKOTNICKI, Adrian John GIBBS, 54 A'Beckett Street, Watson, ACT 2600, Australia assigned to THE A U S T R A L I A N NATIONAL UNIVERSITY A method of direct insertion of double-stranded RNA into a cloning vector consisting of doublestranded DNA, the method comprising: a) isolating the dsRNA from a source of interest: b) if required, decapping the dsRNA: and c) directly ligating the dsRna to the dsDNA of the appropriate cloning vector. This method allows dsRNA to be cloned directly, thus eliminating the traditional step of first making a complementary DNA copy of the RNA molecule. Using this method, it is possible to construct probes for the detection of virus diseases in plants and animals. The method can also be used to produce new plant strains which are resistant to a particular virus, or to provide immunity to animals to a specific virus.
8504899 METHODS AND VECTORS FOR TRANSFORMATION OF PLANT CELLS David H GELFAND, Kenneth A BARTON assigned to CETUS CORPORATION: AGRACETUS
NOVEL DNA AND ITS USE Masaharu SENOO, Haruo ONDA, Koichi IGARASHL D73-106, 18 Tsukumodai 5chome. Suita-shi, Osaka 565, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD: A novel DNA is prepared in which a DNA fraction having a structural gene which code a peptide containing an antibody recognition site and a DNA fraction having a structural gene which code human interleukin 2 peptide are linked to each other. This novel DNA makes it possible to produce a polypeptide in which the two peptides are linked to each other by transforming host microorganism with the DNA and culturing the transformed microorganism.
Vectors and methods suitable for both direct and Agrobacterium)-mediated transformation of plants. The vectors comprise easily manipulated expression cassettes and optionally contain border sequences associated with Agrobacterium) plasmid DNA transfer. By using an intermediate carrier vector which contains the requisite expression control sequences in accessible form, a wide variety of single and multiple cassette vectors can be constructed.
8505122 ROTAVIRUS
8504898 AN IMPROVED METHOD OF CLONING DOUBLE-STRANDED RNA, AND ITS USE IN THE PRODUCTION OF VIRAL GENE CLONES
lan Hamilton HOLMES, Michael Leigh DYALL-SMITH. 13 Avenue Athol, Canterbury. VIC 3126, Australia assigned to THE UNIVERSITY OF MELBOURNE A material being a dsRNA gene segment coding Ibr the major outer capsid glycoprotein of a rolavirus.