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8606742 Fused proteine for enzyme immunoassay system
173
PATENT ABSTRACTS QUE; INSTITUT NATIONAL DE SANTE ET DE LA RECHERCHE M
8607061
LA
The diagnostic in vitro of malignant cells originating from the digestive tube comprises contacting a biological taking with a reactant presenting a specific affinity for villine.
WATER SOLUBLE XANTHYLIUM DERIVATIVE SUBSTRATES Gary A MITCHELL. Gerald E JAFFE, Marilyn M SOLORZANO assigned to COULTER ELECTRONICS INC:
8606636 MONOCLONAL
ANTIBODY
Shigeru KUROOKA, Shingo HIRO1SHI, Shigeyuki MATSUYAMA, Toshiaki KANEKO, Noriyuki SUNAHARA, 10-7, Nonaka 3-chome, Fujiidera-shi, Osaka 583, Japan assigned to DAIN1PPON PHARMACEUTICAL CO LTD; SHIRAIMATSU SHINYAKU CO LTD An anti-human pancreatic alpha-amylase monoclonal antibody having the following properties: (a) capable of recognizing human pancreatic alpha-amylase; (b) substantially unable to recognize human salivary alphaamylase; and (c) not deactivating human pancreatic alpha-amylase even after its immunoreaction with said enzyme as the antigen. A method for assaying human pancreatic alphaamylase using the monoclonal antibody, a method for separating human pancreatic alphaamylase from human salivary alpha-amylase, and a reagent therefor are also disclosed. Separation and assaying of human pancreatic alphaamylase and human salivary alpha-amylase in human body liquid such as blood serum serve to diagnose various diseases, particularly pancreatic diseases, 8606742 FUSED PROTEINE FOR ENZYME IMMUNOASSAY SYSTEM Dale B SCHENK, Sharon Kaye SPRATT assigned to CALIFORNIA BIOTECHNOLOGY INC; A fused protein for use in an enzyme immunoassay system. The protein comprises an enzymatically active beta-galactosidase fused, at its C terminus, to an immunologically active peptide. The protein is produced using a plasmid containing a complete beta-galactosidase gene fused, at its 3" end, with an oligonucleotide coding for the peptide. The fused protein is designed for use in a solid-phase enzyme immunoassay system, based on immunospecific binding of the fused protein to a solid support, or in a homogeneous enzyme immunoassay system, based on enzyme inhibition resulting from immunospecific binding of an antibody to the protein.
Water soluble xanthylium derivative substrates of rhodamine (110) and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition oforganic solvent additives and'or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen to monitor the pathways of blood coagulation. 8607093 PROCESS FOR PREPARING HETEROGENIC PROTEIN Hironobu WATANABE. Ryuji HIRAMATSU, Yoshizumi TANABE, Hideyuki ISHIKAWA, Masanori NAGAI, Hirofumi ARIMURA, 4-7-23-1408, Nankonaka, Suminoe-ku. Osaka-shi, Osaka 559, Japan assigned to THE GREEN CROSS CORPORATION A process for preparing a heterogenic protein by culturing a heterogenic protein-producing transformant obtained by gene-controlling technique and recovering a produced heterogenic protein, which process comprises removing an outer cell wall of the transformant containing the protein therein, and solubilizing the protein with a salt dissociating at least one of chloride, thiocyanate, and nitrate ions. This process makes it possible to recover the heterogenic protein in a high yield in the form showing an activity in bioassay.
8607094 BACTERIAL
CELL EXTRACTANT
Michael John (deceased) HARBER assigned to HARBER F E (executor and legal representative: UNIVERSITY OF WALES COLLEGE OF MEDICINE A reagent for extracting an Adenosine Triphosphate from bacterial cells, comprises Benzalkonium chloride or tetradecyltrimethylammonium bromide, together with a calcium chelator in the form of
PATENT ABSTRACTS QUE; INSTITUT NATIONAL DE SANTE ET DE LA RECHERCHE M
8607061
LA
The diagnostic in vitro of malignant cells originating from the digestive tube comprises contacting a biological taking with a reactant presenting a specific affinity for villine.
WATER SOLUBLE XANTHYLIUM DERIVATIVE SUBSTRATES Gary A MITCHELL. Gerald E JAFFE, Marilyn M SOLORZANO assigned to COULTER ELECTRONICS INC:
8606636 MONOCLONAL
ANTIBODY
Shigeru KUROOKA, Shingo HIRO1SHI, Shigeyuki MATSUYAMA, Toshiaki KANEKO, Noriyuki SUNAHARA, 10-7, Nonaka 3-chome, Fujiidera-shi, Osaka 583, Japan assigned to DAIN1PPON PHARMACEUTICAL CO LTD; SHIRAIMATSU SHINYAKU CO LTD An anti-human pancreatic alpha-amylase monoclonal antibody having the following properties: (a) capable of recognizing human pancreatic alpha-amylase; (b) substantially unable to recognize human salivary alphaamylase; and (c) not deactivating human pancreatic alpha-amylase even after its immunoreaction with said enzyme as the antigen. A method for assaying human pancreatic alphaamylase using the monoclonal antibody, a method for separating human pancreatic alphaamylase from human salivary alpha-amylase, and a reagent therefor are also disclosed. Separation and assaying of human pancreatic alphaamylase and human salivary alpha-amylase in human body liquid such as blood serum serve to diagnose various diseases, particularly pancreatic diseases, 8606742 FUSED PROTEINE FOR ENZYME IMMUNOASSAY SYSTEM Dale B SCHENK, Sharon Kaye SPRATT assigned to CALIFORNIA BIOTECHNOLOGY INC; A fused protein for use in an enzyme immunoassay system. The protein comprises an enzymatically active beta-galactosidase fused, at its C terminus, to an immunologically active peptide. The protein is produced using a plasmid containing a complete beta-galactosidase gene fused, at its 3" end, with an oligonucleotide coding for the peptide. The fused protein is designed for use in a solid-phase enzyme immunoassay system, based on immunospecific binding of the fused protein to a solid support, or in a homogeneous enzyme immunoassay system, based on enzyme inhibition resulting from immunospecific binding of an antibody to the protein.
Water soluble xanthylium derivative substrates of rhodamine (110) and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition oforganic solvent additives and'or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen to monitor the pathways of blood coagulation. 8607093 PROCESS FOR PREPARING HETEROGENIC PROTEIN Hironobu WATANABE. Ryuji HIRAMATSU, Yoshizumi TANABE, Hideyuki ISHIKAWA, Masanori NAGAI, Hirofumi ARIMURA, 4-7-23-1408, Nankonaka, Suminoe-ku. Osaka-shi, Osaka 559, Japan assigned to THE GREEN CROSS CORPORATION A process for preparing a heterogenic protein by culturing a heterogenic protein-producing transformant obtained by gene-controlling technique and recovering a produced heterogenic protein, which process comprises removing an outer cell wall of the transformant containing the protein therein, and solubilizing the protein with a salt dissociating at least one of chloride, thiocyanate, and nitrate ions. This process makes it possible to recover the heterogenic protein in a high yield in the form showing an activity in bioassay.
8607094 BACTERIAL
CELL EXTRACTANT
Michael John (deceased) HARBER assigned to HARBER F E (executor and legal representative: UNIVERSITY OF WALES COLLEGE OF MEDICINE A reagent for extracting an Adenosine Triphosphate from bacterial cells, comprises Benzalkonium chloride or tetradecyltrimethylammonium bromide, together with a calcium chelator in the form of