8606835 Method of studying gene expression and of diagnosing disease

152

PATENT ABSTRACTS

8606745

8606835

MOLECULAR CLONING OF CDNA FOR HUMAN FACTOR VIIIR (VON WILLEBRAND FACTOR)

METHOD OF STUDYING GENE EXPRESSION AND OF DIAGNOSING DISEASE

Dennis C LYNCH, David M LIVINGSTON assigned to MELOY LABORATORIES INC;

Hector F DeLUCA, Charles W BISHOP, David A SANTEK. Nancy C KENDRICK assigned to WISCONSIN ALUMNI RESEARCH FOUNDATION

The invention provides in clonable form a cDNA encoding a portion of human Factor VIII-R (von Willebrand Factor), vector containing same, host cells transformed by said vectors and methods for preparing the cDNA and cloning same.

A method for studying gene expression and diagnosing disease utilizing a combination of twodimensional electrophoresis and double label autoradiography for analyzing proteins made as a result of cellular function and which have been separated by the electrophoretic technique. Also disclosed is a fully automatic method for quantitatively analyzing protein spots contained in a developed, double labelled electrophoresis gel.