quency before and after the incubation period will indicate the extent oi" hybridization.
DETERMINATION OF IDENTITY BETWEEN TWO ORGANISMS 8702132 Barry Gordon Dimitri HALL, James Howard SLATER assigned to BIOTECHNICA LIMITED A method of determining whether two organisms are identical comprises subjecting a restriction endonuclease digest ofgenomic DNA of the first organism to electrophoresis: determining the positions of the DNA fragments thus separated which hybridise with one or more labelled DNA probes: and comparing these positions with the positions of DNA fragments binding to the or each probe and produced from genomic DNA of the second organism in like manner: an amount of probe DNA and one or more restriction endonucleases being used such that sufficient bands are revealed by the probing to achieve a sufficiently low probability (X) that the two organisms will have failed to have been distinguished when they appear identical from their band patterns, as determined by: X = F(q), wherein F is a fraction representative of the proportion DNA fragments which are identical between restriction endonuclease digests of genomic DNA of pairs of independentlyobtained organisms of the same species as the first and second organisms and q is the number of positions revealed by the probing.
ELECTROPHORESIS AND VACUUMMOLECULAR TRANSFER APPARATUS Kenneth Winston JONES. Department of Genetics. University of Edinburgh, ]'he Kings Building, West Mains Road, Edinburgh EH9 3JN, United Kingdom An apparatus which includes the combination of a submarine gel tank (10), for the electrophoretic separation of, for example, nucleic acid molecules on agarose slab gels, with a vacuum applying means (11) which transfers the separated molecules from the gel. without further handling, to a filter membrane (13) b5 means of a controlled vacuum
8702133 ELECTROPHORESIS METHOD AND APPARATUS FOR S E P A R A T I N G P A R T I C L E S IN A SEPARATION MEDIUM Edwin SOUTHERN, 30 Staverton Road, Oxford OX2 6X J, United Kingdom assigned to MEDICAL RESEARCH COUNCIL
8702066 PIEZOELECTRIC DEVICE FOR DETECTION OF POLYNUCLEOTIDE HYBRIDIZATION Newton C FAWCETT, Jeffrey A EVANS assigned to THE UNIVERSITY OF SOUTHERN MISSISSIPPI A system for detecting polynucleotide hybridization. A polynucleotide is immobilized on a surface of a piezoelectric crystal. The resonance frequency of the piezoelectric crystal is measured through means for determining the resonant frequency of a piezoelectric crystal. A separate source of polynucleotide is exposed to the polynucleotide-coated piezoelectric crystal for a sufficient length of time and under conditions suitable for hybridization. The resonance frequency of the crystal is then again measured, and the difference between the resonance fre-
Electrophoresis method and apparatus for separating particles in a separation medium (15). Means being provided for driving the particles alternately in different directions in the separation medium (15). The separation medium (15) is rotated at predetermined intervals in a plane between different rotary positions in one and the same electric field.
8702381 METHOD AND APPARATUS FOR C A T A L Y S T C O N T A I N M E N T IN MULTIPHASE MEMBRANE REACTOR SYSTEMS Stephen L MATSON, Stephen L MATSON assigned to SEPRACOR INC: Apparatus for catalysis in multiphase reaction systems in which catalysts are confined within