- Email: [email protected]
966 DETECTION OF PROSTATE CANCER BY URINE PROTEOMICS
Results: $V H[SHFWHG DJH ZDV VLJQLᚏFDQWO\ DVVRFLDWHG ZLWK DQ LQFUHDVHG ULVN RIDOOFDXVHGHDWK ,55 &,ದ S 7KHPHGLDQDJH DW VFUHHQLQJ ZDV \HDUV 4 4 LQ WKH JURXS RI VFUHHQLQJSRVLWLYH DQG ELRSVLHG PHQ \HDUV 4 4 LQ WKRVH QRW ELRSVLHG GHVSLWH WKH LQGLFDWLRQDQG\HDUV 4 4 LQWKHVFUHHQLQJQHJDWLYHJURXS2IWKRVH ZLWKDSRVLWLYHVFUHHQLQJWHVW XQGHUZHQWELRSV\7KHUHZDVQRVWDWLVWLFDOO\ VLJQLᚏFDQWGLᚎHUHQFHLQFXPXODWLYH\HDURYHUDOOPRUWDOLW\UDWHVEHWZHHQWKHWZR JURXSVRIPHQ &,ದIRUVFUHHQLQJSRVLWLYHPHQ &, ದ IRU VFUHHQLQJQHJDWLYH PHQ 1 6 LPSO\LQJ QR H[FHVV PRUWDOLW\ IRU VFUHHQLQJSRVLWLYH PHQ0HQZLWKDELRSV\ LQGLFDWLRQ ZKRGLGQRWXQGHUJR D SURVWDWH ELRSV\ KDG D PRUH WKDQ GRXEOHG ULVN RI DOOFDXVH GHDWK GXULQJ WKH ᚏUVW \HDU ,55 &, ದ S FRPSDUHG WR WKRVH ZLWKRXW WKH LQGLFDWLRQ 0HQ ZKR ZHUH DFWXDOO\ ELRSVLHG KDG KDOI WKH ULVN ,55 &, ದS FRPSDUHGWRWKRVHZLWKRXWWKHLQGLFDWLRQ7KHVHᚏQGLQJV LQGLFDWHDVHOHFWLRQRIKHDOWK\PHQZKRXQGHUJRELRSV\$FXPXODWLYHWRWDORI DQGVFUHHQLQJSRVLWLYHPHQYHUVXVDQG VFUHHQLQJQHJDWLYHPHQGLHG ZLWKLQDQG GD\VDIWHUVFUHHQLQJUHVSHFWLYHO\2QO\RIWKRVHVFUHHQLQJ SRVLWLYHZKRGLHGZLWKLQ GD\VZHUHLQIDFWELRSVLHGDQGLQQRQHRIWKHVHFDVHV DVDQREYLRXVFRPSOLFDWLRQWRWKHELRSV\ Conclusions: 3URVWDWH ELRSV\ LV QRW DVVRFLDWHG ZLWK H[FHVV PRUWDOLW\ DQG IDWDO FRPSOLFDWLRQVVHHPWREHH[WUHPHO\UDUH
966
DETECTION OF PROSTATE CANCER BY URINE PROTEOMICS
5LJDX00RURWH-&RORPH10LU0&%DOOHVWHURV&*DUFLD0 $EDO0&DQDOV)5HYHQWµV-'ROO$ 1 Vall De Hebron Hospital Research Institute, Dept. of Biomedical Research, Barcelona, Spain, 2Vall De Hebron Hospital, Dept. of Urology, Barcelona, Spain, 3 Vall De Hebron Hospital, Dept. of Proteomics, Barcelona, Spain, 4Complejo Hospitalario Universitario De Santiago, Dept. of Translational Oncology Laboratory, Santiago De Compostela, Spain, 5Vall D’Hebron Hospital, Dept. of Proteomics, Barcelona, Spain, 6Vall De Hebron Hospital, Dept. of Biomedical Research, Barcelona, Spain Introduction & Objectives: 3URVWDWHVSHFLᚏFDQWLJHQ 36$ VHUXPPHDVXUHPHQW LQFRPELQDWLRQZLWKDGLJLWDOUHFWDOH[DPLQDWLRQ '5( DQGWUDQVUHFWDOXOWUDVRXQG JXLGHG ELRSV\ 7856 LV FXUUHQWO\ WKH JROG VWDQGDUG IRU SURVWDWH FDQFHU 3&D VFUHHQLQJLQ(XURSH1HYHUWKHOHVV36$DQG'5(ODFNVLJQLᚏFDQWVSHFLᚏFLW\DQG ELRSV\ ODFNV LGHDO VHQVLWLYLW\ DSUR[ IDOVH QHJDWLYHV 7KHUHIRUH DGGLWLRQDO ELRPDUNHUV DUH QHHGHG WR VXSSOHPHQW RU SRWHQWLDOO\ UHSODFH WKH FXUUHQWO\ XVHG GLDJQRVWLFWHFKQLTXHV Material & Methods: 6HFUHWHGSURWHLQVRUWKHLUSURGXFWVFDQEHGHWHFWHGLQYDULRXV ZD\VHJWKHWUDGLWLRQDODQWLERG\EDVHGDVVD\DSSURDFKRUXVLQJQRYHOSURWHRPLF SODWIRUPV 1HZHU VWUDWHJLHV DOORZ D ಫKLJK WKURXJKSXWಬ DSSURDFK IRU ELRPDUNHU VHDUFKLQJZLWKWKHDGYDQWDJHWKDWWKH\FDQSURYLGHTXDQWLWDWLYHLQIRUPDWLRQ+HUH ZH XVHG D WZRGLPHQVLRQDO JHOEDVHG SURWHRPLF DSSURDFK '',*( FRXSOHG ZLWK PDWUL[ DVVLVWHG ODVHU GHVRUSWLRQLRQL]DWLRQ WLPHRIᚐLJKW PDVV VSHFWURPHWU\ 0$/',72) 06 DQG GDWDEDVH PLQLQJ WR LGHQWLI\ QRYHO ELRPDUNHUV RI 3&D LQ XULQHDQGOLTXLGFKURPDWRJUDSK\LQFRQMXQFWLRQZLWKPDVVVSHFWURPHWU\DQGWULSOH TXDGUXSROHPDVVVSHFWURPHWU\ /&0606 WRYDOLGDWHWKHLGHQWLᚏHGSURWHLQVLQD ELJJHUFRKRUW Results: :H LGHQWLᚏHG D SURWHRPLF SURᚏOH RI SRWHQWLDO ELRPDUNHUV IRU WKH GHWHFWLRQ RI SURVWDWH FDQFHU LQ XULQH 7KH PDMRULW\ RI WKHVH SURWHLQV EHORQJHG VHFUHWHGFRPSRQHQWVRIVHYHUDOZHOONQRZQIXQFWLRQDORIFDQFHUDQGLQᚐDPPDWLRQ QHWZRUNVOLNH1)̱%3'*)%˟DQG36$ Conclusions: 7KHVHGDWDGHPRQVWUDWHWKHDELOLW\RISURWHRPLFDQDO\VHVWRUHYHDO QRYHOELRPDUNHUVIRU3&DLQXULQHDQLPSRUWDQWVWHSIRUZDUGLQDGYDQFLQJDFFXUDWH GLDJQRVLVZKLFKLVFXUUHQWO\WKHERWWOHQHFNIRUWKHDELOLW\WRFXUHSDWLHQWVIURP3&D 7KHVHELRPDUNHUVFRXOGEHXVHGWRGHYHORSDVLPSOHGLDJQRVWLFWHVW (/,6$ WREH XWLOL]HGLQKRVSLWDODQGRXWSDWLHQWURXWLQHV
967
PROSTATE CANCER RISK IN THE PSA RANGE 1-4NG/ML IN THE PROSPECTIVE SCREENING STUDY
DQG UHVSHFWLYHO\ ,Q WKH ᚏUVW VFUHHQLQJ URXQG 7586 JXLGHG SURVWDWH ELRSV\ ZDVRᚎHUHGIRUDOOPHQZLWK36$DERYHQJPODQGDVDVLGHVWXG\SURWRFRO LPSOHPHQWHGLQWKHVWVFUHHQLQJURXQGRQO\ DOOPHQLQWKH36$UDQJHQJPO ZHUHRᚎHUHGDELRSV\ZKHQ)75DWLRZDVEHORZ3&DLQFLGHQFHGDWDIRUDOO VFUHHQLQJSDUWLFLSDQWVDUHDYDLODEOHXQWLO6HSWHPEHU7KHGHWHFWLRQUDWHVLQ WKHVWVFUHHQLQJURXQGDQGFXPXODWLYHLQFLGHQFHUDWHVRI3&DZHUHFDOFXODWHG Results: 2XWRI PHQLQWKHQJPO36$UDQJH KDG)75DWLR EHORZDQG DERYH DJUHHGWRXQGHUJRSURVWDWH ELRSV\UHVXOWLQJLQ3&DFDVHV GHWHFWLRQUDWH 'XULQJIXUWKHUIROORZXSWKH FXPXODWLYHLQFLGHQFHUDWHLQFUHDVHGXSWRIRUWKLVJURXS ZKHUHDV IRU WKH )7 5DWLR DERYH WKH FXPXODWLYH LQFLGHQFH UDWH RI ZDV REVHUYHG ,QWHUYDO FDQFHUV RXWVLGH WKH VFUHHQLQJ VWXG\ SURWRFRO FRQVWLWXWHG DQG RIDOO3&DGHWHFWHGLQUHVSHFWLYHJURXSV2XW RIPHQLQWKHQJPO36$UDQJH KDG)75DWLREHORZDQG DERYH DJUHHGIRUSURVWDWHELRSV\7KH3&DGHWHFWLRQ UDWHZDV EHORZDQG DERYH)75DWLRFXWRᚎ UHVSHFWLYHO\'XULQJIXUWKHUIROORZXSWKHFXPXODWLYHLQFLGHQFHUDWHLQFUHDVHGXS WR IRU)75DWLREHORZDQGXSWR IRU)75DWLRDERYH FXWRᚎ,QWHUYDOFDQFHUVFRQVWLWXWHG DQG RIDOO3&D GHWHFWHGLQUHVSHFWLYHJURXSV Conclusions: 7KLVVWXG\VKRZVWKDWWKHUHLVDVXEVWDQWLDOULVNRIKDYLQJELRSV\ GHWHFWDEOH3&DLQORZ36$UDQJHVDQGWKDWWRWDO36$DQG)75DWLRFDQWRVRPH H[WHQW SUHGLFW 3&D ULVN ,Q WKH UDQJH QJPO WKH 3&D ULVN LQ WKH VW VFUHHQLQJ URXQG ZDV VWURQJHU DVVRFLDWHG ZLWK )7 5DWLR EHORZ YV DERYH FXWRᚎ EXW WKLV UDWLR GHFUHDVHG WR GXULQJ \HDUV IROORZXS ,Q WKH UDQJH QJPO WKHFXPXODWLYHULVNUDWLRZDVHYHQKLJKHU EXWDVZHELRSVLHGRQO\PHQZLWK )75DWLREHORZZHDUHWKXVXQDEOHWRSUHGLFWKRZPDQ\RIWKHVHFDQFHUV ZRXOG KDYH HPHUJHG GXULQJ WKH IROORZXS $ FRPSDUDWLYH VWXG\ ZLWK VLPLODU EXW XQELRSVLHGSRSXODWLRQLVQHHGHGWRLQYHVWLJDWHWKLVTXHVWLRQ
968
JAPANESE PROSPECTIVE COHORT STUDY OF SCREENING FOR PROSTATE CANCER (JPSPC): ANALYSES ON COMPLIANCE, CONTAMINATION AND MORTALITY UNTIL 2008 (GUNMA SECTION)
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.ZLDWNRZVNL06HLOHU'%DXPJDUWQHU0%DXHU6.RULWVLDGLV* +XEHU$.XUUHU0/HKPDQQ.5HFNHU) 1 Cantonal Hospital Aarau, Dept. of Urology, Aarau, Switzerland, 2Kantonsspital Aarau Ag, Dept. of Urology, Aarau, Switzerland, 3Kantonsspital Aarau Ag, Center For Laboratory Medicine, Aarau, Switzerland, 4Kantonsspital Aarau Ag, Institute of Pathology, Aarau, Switzerland, 5Kantonsspital Baden, Dept. of Urology, Baden, Switzerland Introduction & Objectives: 7KH DLP RI WKLV VWXG\ ZDV WR LQYHVWLJDWH WKH FXPXODWLYH ULVN RI KDYLQJ SURVWDWH FDQFHU 3&D LQ ORZ 36$ UDQJH QJPO LQ WKHVFUHHQHGSRSXODWLRQ,QDGGLWLRQWKHXWLOLW\RIIUHHWRWRWDO36$UDWLR )75DWLR ZDVLQYHVWLJDWHGLQWKDWVHWWLQJ7KHGDWDZHUHJDWKHUHGSURVSHFWLYHO\ZLWKLQDQ RQJRLQJ3&DVFUHHQLQJWULDOVHWWLQJ Material & Methods: PHQ SDUWLFLSDQWV RI WKH 6ZLVV VLWH $DUDX RI WKH (563&VFUHHQLQJVWXG\XQGHUZHQWWRWDODQGIUHH36$WHVWLQWKHᚏUVWVFUHHQLQJ URXQG EHWZHHQ DQG KDG 36$ EHWZHHQ DQG QJ PODQG EHWZHHQDQG QJPO)UHH36$ZDVPHDVXUHGLQ
Results: 7KHH[SRVXUHUDWHRIVFUHHQLQJLQ,VHVDNLFLW\EHWZHHQDQG ZDV $OWHUQDWLYHO\WKHOHYHORIFRQWDPLQDWLRQRI36$VFUHHQLQJLQ.LU\XFLW\
Eur Urol Suppl 2010;9(2):303
966
DETECTION OF PROSTATE CANCER BY URINE PROTEOMICS
5LJDX00RURWH-&RORPH10LU0&%DOOHVWHURV&*DUFLD0 $EDO0&DQDOV)5HYHQWµV-'ROO$ 1 Vall De Hebron Hospital Research Institute, Dept. of Biomedical Research, Barcelona, Spain, 2Vall De Hebron Hospital, Dept. of Urology, Barcelona, Spain, 3 Vall De Hebron Hospital, Dept. of Proteomics, Barcelona, Spain, 4Complejo Hospitalario Universitario De Santiago, Dept. of Translational Oncology Laboratory, Santiago De Compostela, Spain, 5Vall D’Hebron Hospital, Dept. of Proteomics, Barcelona, Spain, 6Vall De Hebron Hospital, Dept. of Biomedical Research, Barcelona, Spain Introduction & Objectives: 3URVWDWHVSHFLᚏFDQWLJHQ 36$ VHUXPPHDVXUHPHQW LQFRPELQDWLRQZLWKDGLJLWDOUHFWDOH[DPLQDWLRQ '5( DQGWUDQVUHFWDOXOWUDVRXQG JXLGHG ELRSV\ 7856 LV FXUUHQWO\ WKH JROG VWDQGDUG IRU SURVWDWH FDQFHU 3&D VFUHHQLQJLQ(XURSH1HYHUWKHOHVV36$DQG'5(ODFNVLJQLᚏFDQWVSHFLᚏFLW\DQG ELRSV\ ODFNV LGHDO VHQVLWLYLW\ DSUR[ IDOVH QHJDWLYHV 7KHUHIRUH DGGLWLRQDO ELRPDUNHUV DUH QHHGHG WR VXSSOHPHQW RU SRWHQWLDOO\ UHSODFH WKH FXUUHQWO\ XVHG GLDJQRVWLFWHFKQLTXHV Material & Methods: 6HFUHWHGSURWHLQVRUWKHLUSURGXFWVFDQEHGHWHFWHGLQYDULRXV ZD\VHJWKHWUDGLWLRQDODQWLERG\EDVHGDVVD\DSSURDFKRUXVLQJQRYHOSURWHRPLF SODWIRUPV 1HZHU VWUDWHJLHV DOORZ D ಫKLJK WKURXJKSXWಬ DSSURDFK IRU ELRPDUNHU VHDUFKLQJZLWKWKHDGYDQWDJHWKDWWKH\FDQSURYLGHTXDQWLWDWLYHLQIRUPDWLRQ+HUH ZH XVHG D WZRGLPHQVLRQDO JHOEDVHG SURWHRPLF DSSURDFK '',*( FRXSOHG ZLWK PDWUL[ DVVLVWHG ODVHU GHVRUSWLRQLRQL]DWLRQ WLPHRIᚐLJKW PDVV VSHFWURPHWU\ 0$/',72) 06 DQG GDWDEDVH PLQLQJ WR LGHQWLI\ QRYHO ELRPDUNHUV RI 3&D LQ XULQHDQGOLTXLGFKURPDWRJUDSK\LQFRQMXQFWLRQZLWKPDVVVSHFWURPHWU\DQGWULSOH TXDGUXSROHPDVVVSHFWURPHWU\ /&0606 WRYDOLGDWHWKHLGHQWLᚏHGSURWHLQVLQD ELJJHUFRKRUW Results: :H LGHQWLᚏHG D SURWHRPLF SURᚏOH RI SRWHQWLDO ELRPDUNHUV IRU WKH GHWHFWLRQ RI SURVWDWH FDQFHU LQ XULQH 7KH PDMRULW\ RI WKHVH SURWHLQV EHORQJHG VHFUHWHGFRPSRQHQWVRIVHYHUDOZHOONQRZQIXQFWLRQDORIFDQFHUDQGLQᚐDPPDWLRQ QHWZRUNVOLNH1)̱%3'*)%˟DQG36$ Conclusions: 7KHVHGDWDGHPRQVWUDWHWKHDELOLW\RISURWHRPLFDQDO\VHVWRUHYHDO QRYHOELRPDUNHUVIRU3&DLQXULQHDQLPSRUWDQWVWHSIRUZDUGLQDGYDQFLQJDFFXUDWH GLDJQRVLVZKLFKLVFXUUHQWO\WKHERWWOHQHFNIRUWKHDELOLW\WRFXUHSDWLHQWVIURP3&D 7KHVHELRPDUNHUVFRXOGEHXVHGWRGHYHORSDVLPSOHGLDJQRVWLFWHVW (/,6$ WREH XWLOL]HGLQKRVSLWDODQGRXWSDWLHQWURXWLQHV
967
PROSTATE CANCER RISK IN THE PSA RANGE 1-4NG/ML IN THE PROSPECTIVE SCREENING STUDY
DQG UHVSHFWLYHO\ ,Q WKH ᚏUVW VFUHHQLQJ URXQG 7586 JXLGHG SURVWDWH ELRSV\ ZDVRᚎHUHGIRUDOOPHQZLWK36$DERYHQJPODQGDVDVLGHVWXG\SURWRFRO LPSOHPHQWHGLQWKHVWVFUHHQLQJURXQGRQO\ DOOPHQLQWKH36$UDQJHQJPO ZHUHRᚎHUHGDELRSV\ZKHQ)75DWLRZDVEHORZ3&DLQFLGHQFHGDWDIRUDOO VFUHHQLQJSDUWLFLSDQWVDUHDYDLODEOHXQWLO6HSWHPEHU7KHGHWHFWLRQUDWHVLQ WKHVWVFUHHQLQJURXQGDQGFXPXODWLYHLQFLGHQFHUDWHVRI3&DZHUHFDOFXODWHG Results: 2XWRI PHQLQWKHQJPO36$UDQJH KDG)75DWLR EHORZDQG DERYH DJUHHGWRXQGHUJRSURVWDWH ELRSV\UHVXOWLQJLQ3&DFDVHV GHWHFWLRQUDWH 'XULQJIXUWKHUIROORZXSWKH FXPXODWLYHLQFLGHQFHUDWHLQFUHDVHGXSWRIRUWKLVJURXS ZKHUHDV IRU WKH )7 5DWLR DERYH WKH FXPXODWLYH LQFLGHQFH UDWH RI ZDV REVHUYHG ,QWHUYDO FDQFHUV RXWVLGH WKH VFUHHQLQJ VWXG\ SURWRFRO FRQVWLWXWHG DQG RIDOO3&DGHWHFWHGLQUHVSHFWLYHJURXSV2XW RIPHQLQWKHQJPO36$UDQJH KDG)75DWLREHORZDQG DERYH DJUHHGIRUSURVWDWHELRSV\7KH3&DGHWHFWLRQ UDWHZDV EHORZDQG DERYH)75DWLRFXWRᚎ UHVSHFWLYHO\'XULQJIXUWKHUIROORZXSWKHFXPXODWLYHLQFLGHQFHUDWHLQFUHDVHGXS WR IRU)75DWLREHORZDQGXSWR IRU)75DWLRDERYH FXWRᚎ,QWHUYDOFDQFHUVFRQVWLWXWHG DQG RIDOO3&D GHWHFWHGLQUHVSHFWLYHJURXSV Conclusions: 7KLVVWXG\VKRZVWKDWWKHUHLVDVXEVWDQWLDOULVNRIKDYLQJELRSV\ GHWHFWDEOH3&DLQORZ36$UDQJHVDQGWKDWWRWDO36$DQG)75DWLRFDQWRVRPH H[WHQW SUHGLFW 3&D ULVN ,Q WKH UDQJH QJPO WKH 3&D ULVN LQ WKH VW VFUHHQLQJ URXQG ZDV VWURQJHU DVVRFLDWHG ZLWK )7 5DWLR EHORZ YV DERYH FXWRᚎ EXW WKLV UDWLR GHFUHDVHG WR GXULQJ \HDUV IROORZXS ,Q WKH UDQJH QJPO WKHFXPXODWLYHULVNUDWLRZDVHYHQKLJKHU EXWDVZHELRSVLHGRQO\PHQZLWK )75DWLREHORZZHDUHWKXVXQDEOHWRSUHGLFWKRZPDQ\RIWKHVHFDQFHUV ZRXOG KDYH HPHUJHG GXULQJ WKH IROORZXS $ FRPSDUDWLYH VWXG\ ZLWK VLPLODU EXW XQELRSVLHGSRSXODWLRQLVQHHGHGWRLQYHVWLJDWHWKLVTXHVWLRQ
968
JAPANESE PROSPECTIVE COHORT STUDY OF SCREENING FOR PROSTATE CANCER (JPSPC): ANALYSES ON COMPLIANCE, CONTAMINATION AND MORTALITY UNTIL 2008 (GUNMA SECTION)
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.ZLDWNRZVNL06HLOHU'%DXPJDUWQHU0%DXHU6.RULWVLDGLV* +XEHU$.XUUHU0/HKPDQQ.5HFNHU) 1 Cantonal Hospital Aarau, Dept. of Urology, Aarau, Switzerland, 2Kantonsspital Aarau Ag, Dept. of Urology, Aarau, Switzerland, 3Kantonsspital Aarau Ag, Center For Laboratory Medicine, Aarau, Switzerland, 4Kantonsspital Aarau Ag, Institute of Pathology, Aarau, Switzerland, 5Kantonsspital Baden, Dept. of Urology, Baden, Switzerland Introduction & Objectives: 7KH DLP RI WKLV VWXG\ ZDV WR LQYHVWLJDWH WKH FXPXODWLYH ULVN RI KDYLQJ SURVWDWH FDQFHU 3&D LQ ORZ 36$ UDQJH QJPO LQ WKHVFUHHQHGSRSXODWLRQ,QDGGLWLRQWKHXWLOLW\RIIUHHWRWRWDO36$UDWLR )75DWLR ZDVLQYHVWLJDWHGLQWKDWVHWWLQJ7KHGDWDZHUHJDWKHUHGSURVSHFWLYHO\ZLWKLQDQ RQJRLQJ3&DVFUHHQLQJWULDOVHWWLQJ Material & Methods: PHQ SDUWLFLSDQWV RI WKH 6ZLVV VLWH $DUDX RI WKH (563&VFUHHQLQJVWXG\XQGHUZHQWWRWDODQGIUHH36$WHVWLQWKHᚏUVWVFUHHQLQJ URXQG EHWZHHQ DQG KDG 36$ EHWZHHQ DQG QJ PODQG EHWZHHQDQG QJPO)UHH36$ZDVPHDVXUHGLQ
Results: 7KHH[SRVXUHUDWHRIVFUHHQLQJLQ,VHVDNLFLW\EHWZHHQDQG ZDV $OWHUQDWLYHO\WKHOHYHORIFRQWDPLQDWLRQRI36$VFUHHQLQJLQ.LU\XFLW\
Eur Urol Suppl 2010;9(2):303