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A COLOUR REACTION FOR BENZEDRINE
1275
CLINICAL AND LABORATORY NOTES A COLOUR REACTION FOR BENZEDRINE BY DEREK
RICHTER, M.A., Ph.D.
Central Pathological Laboratory of the London County Hospitals for Nervous and Mental Disorders)
(From the
IN some subjects the effects of benzedrine appear to be transient, lasting only a few hours, while in In others they may be felt for more than a day. order to obtain more information as to the duration of the action of benzedrine the rate of excretion in the urine has been measured. Using the micromethod described below it was found that with doses of 20 mg. benzedrine sulphate in some subjects only 30-50 per cent. is excreted in the urine in 24 hours and benzedrine may still be detected in the urine 40 hours after administration. a number of this may be estimation for
deformity in order to allow for the contraction of scar tissue. The increased width of the base of the nostril is corrected in the ordinary way by resection of tissue and undermining. It is with the flattened portion on one side of the tip that we are concerned, and the object of the operation is to fold a portion of the alar cartilage of the normal side over to the flattened side after freeing the flattened cartilage from the skin above it; in this way the bent cartilage, by its natural elasticity, supports the flattened nostril. the
correct
subsequent
Benzedrine gives a yellow colour with nitrophenols, including picric acid, and used
a as convenient method of amounts down to 0-001 mg. per c.cm. To 60 c.cm. urine in a 250 c.cm. separating funnel is added 6 c.cm. petroleum ether and 4 c.cm. 2N caustic soda solution. The mixture is shaken three minutes by a rotating motion to prevent froth formation, allowed to stand two minutes, and the aqueous layer run off. The petroleum ether layer is centrifuged five minutes, cleared by stirring with a glass rod, and centrifuged for a further two minutes. 3 c.cm. of the petroleum ether solution is measured in a dry pipette or 10 c.cm. measuring cylinder and washed with 3 c.cm. chloroform into a dry test-tube. 0’6 c.cm. of a solution containing 1 per cent. picric acid in toluene is now added. The tube is allowed to stand well corked for 12 hours during which time the picrates of other basic substances from the urine separate out ; the clear yellow solution is then compared with a series of standards prepared in a similar manner from solutions containing 0, 1, 2, 5, 10, and 20 y benzedrine per c.cm. For making the standards pure liquid benzedrine was used and the solution titrated with standard N/5 acid, but for many purposes it is sufficiently accurate to use a solution obtained by heating a 10 mg. tablet of benzedrine sulphate with 7’35 c.cm. water (the starch with which The the tablets are made up does not interfere). resulting solution contains 1 mg. base per c.cm. Normal urines often contain small amounts of amines which give a slight blank value corresponding to about 0-5-1-0 y benzedrine per c.cm. The value of the blank must therefore be determined and subtracted from the values obtained after giving benzedrine. Small amounts of indole in the urine do not interfere with the estimation, but the formation of amines by bacterial decomposition must be avoided by adding a few drops of toluene and using the urines as fresh as possible. Traces of moisture or alkali in the tubes interfere with the estimation and it is essential that the tubes should be well cleaned with cleaning fluid and dried. If four separating funnels are used a large number of estimations may be made in a comparatively short time.
THE NOSTRIL IN
SECONDARY HARE-LIP
BY GRAHAM HUMBY, M.R.C.S.
Eng.
THE following operation is offered as an alternative to existing methods for the correction of the flattened nostril in secondary hare-lip. I believe that it gives a more positive result and enables one to over-
The deformity.
Desideratum.
The knife is inserted at the lower edge of the flattened cartilage, and the freeing is done parallel to the superficial surface of it-i.e., between the cartilage and the skin. On the normal side, the lateral portion of the alar cartilage is resected submucously and folded over into the space which has been created on the flattened side between the cartilage and skin. No stitches are necessary.
Sub mucous resection.
Cartilage folded
over.
Should it be feared that the normal nostril will when bereft of its cartilage, a narrow margin of its lower border may be left in situ and not swung over with the rest to the other side ; but the normal nostril tip is usually unduly prominent and a slight collapse does not detract from the final result. The manipulation of the alar cartilages does not involve any interference with the nasal septum. Only a few cases have been submitted to this procedure, but the results obtained justify this communication.
collapse
IDIOSYNCRASY TO ACRIFLAVINE BY W. A. YOUNG, B.M. Oxon., B.Sc.Lond., D.T.M.&H. SENIOR MEDICAL
F.
OFFICER, SEWA HADJI HOSPITAL, DAR-ES-SALAAM, TANGANYIKA TERRITORY ; AND
HAWKING, D.M. Oxon., D.T.M.
SENIOR FELLOW IN TROPICAL MEDICINE, MEDICAL RESEARCH COUNCIL
THE purpose of this communication is briefly to record a case of skin sensitivity to acriflavine. On March 23rd, 1938, an Indian aged 38 was admitted to the Sewa Hadji Hospital for fever, vomiting, and bronchitis. He also had a chronicsinus in the left upper arm which had followed injury to the arm two years previously. The Wassermann reaction A few days after admission the sinus was negative.
CLINICAL AND LABORATORY NOTES A COLOUR REACTION FOR BENZEDRINE BY DEREK
RICHTER, M.A., Ph.D.
Central Pathological Laboratory of the London County Hospitals for Nervous and Mental Disorders)
(From the
IN some subjects the effects of benzedrine appear to be transient, lasting only a few hours, while in In others they may be felt for more than a day. order to obtain more information as to the duration of the action of benzedrine the rate of excretion in the urine has been measured. Using the micromethod described below it was found that with doses of 20 mg. benzedrine sulphate in some subjects only 30-50 per cent. is excreted in the urine in 24 hours and benzedrine may still be detected in the urine 40 hours after administration. a number of this may be estimation for
deformity in order to allow for the contraction of scar tissue. The increased width of the base of the nostril is corrected in the ordinary way by resection of tissue and undermining. It is with the flattened portion on one side of the tip that we are concerned, and the object of the operation is to fold a portion of the alar cartilage of the normal side over to the flattened side after freeing the flattened cartilage from the skin above it; in this way the bent cartilage, by its natural elasticity, supports the flattened nostril. the
correct
subsequent
Benzedrine gives a yellow colour with nitrophenols, including picric acid, and used
a as convenient method of amounts down to 0-001 mg. per c.cm. To 60 c.cm. urine in a 250 c.cm. separating funnel is added 6 c.cm. petroleum ether and 4 c.cm. 2N caustic soda solution. The mixture is shaken three minutes by a rotating motion to prevent froth formation, allowed to stand two minutes, and the aqueous layer run off. The petroleum ether layer is centrifuged five minutes, cleared by stirring with a glass rod, and centrifuged for a further two minutes. 3 c.cm. of the petroleum ether solution is measured in a dry pipette or 10 c.cm. measuring cylinder and washed with 3 c.cm. chloroform into a dry test-tube. 0’6 c.cm. of a solution containing 1 per cent. picric acid in toluene is now added. The tube is allowed to stand well corked for 12 hours during which time the picrates of other basic substances from the urine separate out ; the clear yellow solution is then compared with a series of standards prepared in a similar manner from solutions containing 0, 1, 2, 5, 10, and 20 y benzedrine per c.cm. For making the standards pure liquid benzedrine was used and the solution titrated with standard N/5 acid, but for many purposes it is sufficiently accurate to use a solution obtained by heating a 10 mg. tablet of benzedrine sulphate with 7’35 c.cm. water (the starch with which The the tablets are made up does not interfere). resulting solution contains 1 mg. base per c.cm. Normal urines often contain small amounts of amines which give a slight blank value corresponding to about 0-5-1-0 y benzedrine per c.cm. The value of the blank must therefore be determined and subtracted from the values obtained after giving benzedrine. Small amounts of indole in the urine do not interfere with the estimation, but the formation of amines by bacterial decomposition must be avoided by adding a few drops of toluene and using the urines as fresh as possible. Traces of moisture or alkali in the tubes interfere with the estimation and it is essential that the tubes should be well cleaned with cleaning fluid and dried. If four separating funnels are used a large number of estimations may be made in a comparatively short time.
THE NOSTRIL IN
SECONDARY HARE-LIP
BY GRAHAM HUMBY, M.R.C.S.
Eng.
THE following operation is offered as an alternative to existing methods for the correction of the flattened nostril in secondary hare-lip. I believe that it gives a more positive result and enables one to over-
The deformity.
Desideratum.
The knife is inserted at the lower edge of the flattened cartilage, and the freeing is done parallel to the superficial surface of it-i.e., between the cartilage and the skin. On the normal side, the lateral portion of the alar cartilage is resected submucously and folded over into the space which has been created on the flattened side between the cartilage and skin. No stitches are necessary.
Sub mucous resection.
Cartilage folded
over.
Should it be feared that the normal nostril will when bereft of its cartilage, a narrow margin of its lower border may be left in situ and not swung over with the rest to the other side ; but the normal nostril tip is usually unduly prominent and a slight collapse does not detract from the final result. The manipulation of the alar cartilages does not involve any interference with the nasal septum. Only a few cases have been submitted to this procedure, but the results obtained justify this communication.
collapse
IDIOSYNCRASY TO ACRIFLAVINE BY W. A. YOUNG, B.M. Oxon., B.Sc.Lond., D.T.M.&H. SENIOR MEDICAL
F.
OFFICER, SEWA HADJI HOSPITAL, DAR-ES-SALAAM, TANGANYIKA TERRITORY ; AND
HAWKING, D.M. Oxon., D.T.M.
SENIOR FELLOW IN TROPICAL MEDICINE, MEDICAL RESEARCH COUNCIL
THE purpose of this communication is briefly to record a case of skin sensitivity to acriflavine. On March 23rd, 1938, an Indian aged 38 was admitted to the Sewa Hadji Hospital for fever, vomiting, and bronchitis. He also had a chronicsinus in the left upper arm which had followed injury to the arm two years previously. The Wassermann reaction A few days after admission the sinus was negative.