- Email: [email protected]
A comparative study of open and laparoscopic radical antegrade modular pancreatosplenectomy versus conventional distal pancreatosplenectomy for left-sided pancreatic ductal adenocarcinoma
Abstracts / Pancreatology 16 (2016) S1eS63
Conclusions: Tamoxifen repressed PKC pathway in pancreatic cancer cells and repressed cell growth through a PKC-dependent and ER-independent manner. Keywords: Tamoxifen; Pancreatic cancer; PKC
S39
enhances nerve outgrowth to pancreatic cancer cell colonies in an in vitro 3D model of nerve invasion in cancer. Furthermore, upregulated Nodal expression enhanced tumor cell invasion of the trunk and nerve dysfunction along sciatic nerves and also promoted orthotropic xenograft tumor growth in vivo. Conclusions: Our study indicates that Nodal is involved in tumor invasion via mediating nerve-tumor interaction. Inhibiting the expression of Nodal could be a potential strategy targeting PNI in pancreatic cancer therapy.
15429. Netrin-1 up-regulates SENP1 expression in pancreatic cancer cells through activation of NF-kB 15433.
A.A. Liu, J. Chen, L.G. Shi, D.L. Chen, L. Tang, C.H. Shao*
A comparative study of open and laparoscopic radical antegrade modular pancreatosplenectomy versus conventional distal pancreatosplenectomy for left-sided pancreatic ductal adenocarcinoma
Department of General Surgery (Department of Pancreatic-biliary Surgery), Changzheng Hospital, Second Military Medical University, Shanghai, China
Menghua Dai*, #, Guangdong Wu #, Yatong Li, Ning Shi, Taiping Zhang, Quan Liao, Ziwen Liu, Junchao Guo, Wenming Wu, Ge Chen, Ya Hu, Yupei Zhao*
Abstract Background: Netrin-1 displays proto-oncogenic activity in several cancers, and exerts its biological effects through binding to its receptors.Netrin-1 is up-regulated in several types of cancer tissues and either knockdown of its expression or inhibition of its receptors result in the tumor regression.SENP1 encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family.SENP1 is involved in the pathogenesis of tumorigenesis and its expression levels are elevated in several types of cancers, we investigate the roles of Netrin-1 on the regulation of SENP1 in pancreatic cancer cells. Methods: In the present study, we determined the role of Netrin-1 on SENP1 expression in the pancreatic cancer cell line PANC-1 and CFPAC-1 cells by RNA isolation, Real-time PCR, Western blot analysis, Small interfering RNA, Dual luciferase reporter assays and Chromatin immunoprecipitation assays. Results: Our results revealed that Netrin-1 overexpression promoted while its knockdown inhibited SENP1 expression in two pancreatic cancer cells. At the molecular level, we found that Netrin-1 activates NF-kB signaling pathway and regulates SENP1 expression through NF-kB signaling pathway. Conclusion: Our studies demonstrate a previous unknown Netrin-1/ SENP1 network in pancreatic cancer cells, suggesting that the abnormal function of Netrin-1 could be an important mechanism for the initiation and progression of pancreatic cancer
15431. Overexpression of nodal enhances perineural invasion in pancreatic cancer by promoting tumor-neural convergence
Department of General Sugery, Peking Union Medical Colleage Hospital, Chinese Academy of Medical Science, Beijing, China, 100730 E-mail address: [email protected], [email protected]
Objective: This study was designed to determine whether Radical antegrade modular pancreatosplenectomy (RAMPS) could improve resection margin status and number of lymph node resected. This study also would prove whether laparoscopic (lap) RAMPS for left-sided pancreatic ductal adenocarcinoma (PDAC) is feasible and safe. Method: Data from 36 patients performed RAMPS were collected from June 2009 to October 2014, 8 of them performed lap RAMPS. 83 patients performed conventional distal pancreatosplenectomy (CDP) for left-side PDAC at the same duration were as case-control to compare clinicopathological factors and oncological outcomes. We also compared these variables between lap and open RAMPS. Result: In RAMPS group, the median number of lymph nodes resected was 14. There was a significant difference in number of lymph nodes resected (14 vs 8; p¼0.000) for RAMPS and CDP. On survival analysis, median survival of 24.53 months in RAMPS group was better than that of 21.00 months in CDP group (p¼0.096). Surgical procedure (RAMPS or CDP), grade of differentiation, TNM stage and resection margin status were independent factors for postoperative prognosis of patients by multivariate analysis (p¼0.036, p¼0.002, p¼0.009, p¼0.002).The median length of hospital stay (LOS) and postoperative time to recovering diet in lap RAMPS group were 17 and 3.5 days, which were significant shorter than in the open RAMPS group (19.5 and 6 days), (p¼0.045, p¼0.004). Conclusion: RAMPS increased the number of lymph node resected and was prognostic factor for improved survival in left-sided PDAC patients. Lap RAMPS was feasible and safe for the left-side PDAC with short LOS and postoperative time to recovering diet.
Jiahui Li, Zheng Wang, Zheng Wu, Qingyong Ma
Keywords: Left-sided PDAC; RAMPS; Lap RAMPS
Department of Hepatobiliary and Pancreas Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China Aim: To explore the contribution of Nodal in pancreatic cancer Perineural invasion (PNI) and progression. Background: PNI is a characteristic feature for local spread of pancreatic cancer. Nodal, an embryonic protein plays a critical role in embryonic neural development, overexpressed in human pancreatic cancer. Methods: We evaluated the function of Nodal in PNI progression by conducting rat dorsal root ganglia and pancreatic cancer cells co-culture in vitro, cellular and molecular biology, and animal models. Results: We find that overexpression of Nodal in tumor cells contribute to PNI in pancreatic cancer and can serve as a marker for progression of pancreatic cancer. Moreover, the overexpression of Nodal leads to promotion of pancreatic cancer cell migration along nerve axons and upregulating expression of NGF, BDNF and GDNF in tumor cells, which
15434. A comparative study of EUS, CT, and MRCP to predict malignancy of branch-duct type intraductal papillary mucinous neoplasm Wu Dong, Guo Tao, Wu Xi, Yao Fang, Yang Aiming*, Qian Jiaming Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China E-mail address: [email protected]
* Corresponding authors. These authors contributed equally to this work and should be considered cofirst authors. * Corresponding author. #
* Corresponding author.
Conclusions: Tamoxifen repressed PKC pathway in pancreatic cancer cells and repressed cell growth through a PKC-dependent and ER-independent manner. Keywords: Tamoxifen; Pancreatic cancer; PKC
S39
enhances nerve outgrowth to pancreatic cancer cell colonies in an in vitro 3D model of nerve invasion in cancer. Furthermore, upregulated Nodal expression enhanced tumor cell invasion of the trunk and nerve dysfunction along sciatic nerves and also promoted orthotropic xenograft tumor growth in vivo. Conclusions: Our study indicates that Nodal is involved in tumor invasion via mediating nerve-tumor interaction. Inhibiting the expression of Nodal could be a potential strategy targeting PNI in pancreatic cancer therapy.
15429. Netrin-1 up-regulates SENP1 expression in pancreatic cancer cells through activation of NF-kB 15433.
A.A. Liu, J. Chen, L.G. Shi, D.L. Chen, L. Tang, C.H. Shao*
A comparative study of open and laparoscopic radical antegrade modular pancreatosplenectomy versus conventional distal pancreatosplenectomy for left-sided pancreatic ductal adenocarcinoma
Department of General Surgery (Department of Pancreatic-biliary Surgery), Changzheng Hospital, Second Military Medical University, Shanghai, China
Menghua Dai*, #, Guangdong Wu #, Yatong Li, Ning Shi, Taiping Zhang, Quan Liao, Ziwen Liu, Junchao Guo, Wenming Wu, Ge Chen, Ya Hu, Yupei Zhao*
Abstract Background: Netrin-1 displays proto-oncogenic activity in several cancers, and exerts its biological effects through binding to its receptors.Netrin-1 is up-regulated in several types of cancer tissues and either knockdown of its expression or inhibition of its receptors result in the tumor regression.SENP1 encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family.SENP1 is involved in the pathogenesis of tumorigenesis and its expression levels are elevated in several types of cancers, we investigate the roles of Netrin-1 on the regulation of SENP1 in pancreatic cancer cells. Methods: In the present study, we determined the role of Netrin-1 on SENP1 expression in the pancreatic cancer cell line PANC-1 and CFPAC-1 cells by RNA isolation, Real-time PCR, Western blot analysis, Small interfering RNA, Dual luciferase reporter assays and Chromatin immunoprecipitation assays. Results: Our results revealed that Netrin-1 overexpression promoted while its knockdown inhibited SENP1 expression in two pancreatic cancer cells. At the molecular level, we found that Netrin-1 activates NF-kB signaling pathway and regulates SENP1 expression through NF-kB signaling pathway. Conclusion: Our studies demonstrate a previous unknown Netrin-1/ SENP1 network in pancreatic cancer cells, suggesting that the abnormal function of Netrin-1 could be an important mechanism for the initiation and progression of pancreatic cancer
15431. Overexpression of nodal enhances perineural invasion in pancreatic cancer by promoting tumor-neural convergence
Department of General Sugery, Peking Union Medical Colleage Hospital, Chinese Academy of Medical Science, Beijing, China, 100730 E-mail address: [email protected], [email protected]
Objective: This study was designed to determine whether Radical antegrade modular pancreatosplenectomy (RAMPS) could improve resection margin status and number of lymph node resected. This study also would prove whether laparoscopic (lap) RAMPS for left-sided pancreatic ductal adenocarcinoma (PDAC) is feasible and safe. Method: Data from 36 patients performed RAMPS were collected from June 2009 to October 2014, 8 of them performed lap RAMPS. 83 patients performed conventional distal pancreatosplenectomy (CDP) for left-side PDAC at the same duration were as case-control to compare clinicopathological factors and oncological outcomes. We also compared these variables between lap and open RAMPS. Result: In RAMPS group, the median number of lymph nodes resected was 14. There was a significant difference in number of lymph nodes resected (14 vs 8; p¼0.000) for RAMPS and CDP. On survival analysis, median survival of 24.53 months in RAMPS group was better than that of 21.00 months in CDP group (p¼0.096). Surgical procedure (RAMPS or CDP), grade of differentiation, TNM stage and resection margin status were independent factors for postoperative prognosis of patients by multivariate analysis (p¼0.036, p¼0.002, p¼0.009, p¼0.002).The median length of hospital stay (LOS) and postoperative time to recovering diet in lap RAMPS group were 17 and 3.5 days, which were significant shorter than in the open RAMPS group (19.5 and 6 days), (p¼0.045, p¼0.004). Conclusion: RAMPS increased the number of lymph node resected and was prognostic factor for improved survival in left-sided PDAC patients. Lap RAMPS was feasible and safe for the left-side PDAC with short LOS and postoperative time to recovering diet.
Jiahui Li, Zheng Wang, Zheng Wu, Qingyong Ma
Keywords: Left-sided PDAC; RAMPS; Lap RAMPS
Department of Hepatobiliary and Pancreas Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China Aim: To explore the contribution of Nodal in pancreatic cancer Perineural invasion (PNI) and progression. Background: PNI is a characteristic feature for local spread of pancreatic cancer. Nodal, an embryonic protein plays a critical role in embryonic neural development, overexpressed in human pancreatic cancer. Methods: We evaluated the function of Nodal in PNI progression by conducting rat dorsal root ganglia and pancreatic cancer cells co-culture in vitro, cellular and molecular biology, and animal models. Results: We find that overexpression of Nodal in tumor cells contribute to PNI in pancreatic cancer and can serve as a marker for progression of pancreatic cancer. Moreover, the overexpression of Nodal leads to promotion of pancreatic cancer cell migration along nerve axons and upregulating expression of NGF, BDNF and GDNF in tumor cells, which
15434. A comparative study of EUS, CT, and MRCP to predict malignancy of branch-duct type intraductal papillary mucinous neoplasm Wu Dong, Guo Tao, Wu Xi, Yao Fang, Yang Aiming*, Qian Jiaming Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China E-mail address: [email protected]
* Corresponding authors. These authors contributed equally to this work and should be considered cofirst authors. * Corresponding author. #
* Corresponding author.