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A cytogenetic study of breast cancer patients
158
Abstracts
A CYTOGENETIC STUDY OF BREAST CANCER PATIENTS Miriana Koncar Mubrin Dept. Nucl. Med. Oncol., Univ. Hosp. "Sestre Milosrdnice", Zagreb, Croatia. Genetic instability, expressed as chromosome instability is known to exist in a variety of patients affected by cancer. Latent instability, unrecognizable by cytogenetic methods may also exist. In the lymphocytes from six operated, otherwise untreated patients with invasive ductal carcinoma of the breast (stage I and II), a cytogenetic analysis demonstrated no common constitutional abnormality, but tentatively indicated a hot spot on 16q, where in minority of metaphases three patients had a fragile site. A month after radiotherapy (clastogenic effect), repeated analysis revealed chromosome arms lp, 4q, 5q, 6p, 7q and again 16q as most frequently involved in structural changes, possibly unmasking instability and/or revealing some of "susceptibility loci". Additional clonal structural abnormalities (i.e. ring chromosomes, markers originating from chromosomes 3 and 13, numerous dmin's) were found two months after radiotherapy with no other treatment. Obtained chromosomal abnormalities indicate joint effect of predetermined instability and irradiation treatment. It seems that complex intercorrelation of clinical status, treatment effect, length of survival, outcome etc., with chromosomal abnormalities during long-term follow-up of individual patient might explain practical significance of chromosomal changes during the course of disease.
CHROMOSOME STUDIES ON 45 PRIMARY BREAST CARCINOMAS. L. Romitti*, S.G. Carinelli** *LABORATORIO DI CITOGENETICA and **LABORATORIO DI ISTOLOGIA ED ANATOMIA PATOLOGICA, ISTITUTI CLINICI DI PERFEZIONAMENTO,MILANO Cytogenetic analysis was performed on 45 primary breast cancer, 75% of which was represented by infiltrating ductal carcinomas. In order to obtain good metaphase quality, tissue cultures were set up. Chromosome rearrangements and aneuploidies were detected in 10 cases whereas the remaining cases showed a normal diploid karyotype. No recurrent changes were identified and the presence of gene amplification was observed in 6 cases as homogeneous staining regions while no double minutes were detected. Although some authors claimed that a relevant fraction of breast cancers appears to be composed predominantly of diploid cell on the basis of flow cytometry and cytogenetic studies, the detection of a normal karyotype could be related to the desmoplasia, excessive connective tissue stroma, associated with about 75% of breast carcinomas.
Abstracts
A CYTOGENETIC STUDY OF BREAST CANCER PATIENTS Miriana Koncar Mubrin Dept. Nucl. Med. Oncol., Univ. Hosp. "Sestre Milosrdnice", Zagreb, Croatia. Genetic instability, expressed as chromosome instability is known to exist in a variety of patients affected by cancer. Latent instability, unrecognizable by cytogenetic methods may also exist. In the lymphocytes from six operated, otherwise untreated patients with invasive ductal carcinoma of the breast (stage I and II), a cytogenetic analysis demonstrated no common constitutional abnormality, but tentatively indicated a hot spot on 16q, where in minority of metaphases three patients had a fragile site. A month after radiotherapy (clastogenic effect), repeated analysis revealed chromosome arms lp, 4q, 5q, 6p, 7q and again 16q as most frequently involved in structural changes, possibly unmasking instability and/or revealing some of "susceptibility loci". Additional clonal structural abnormalities (i.e. ring chromosomes, markers originating from chromosomes 3 and 13, numerous dmin's) were found two months after radiotherapy with no other treatment. Obtained chromosomal abnormalities indicate joint effect of predetermined instability and irradiation treatment. It seems that complex intercorrelation of clinical status, treatment effect, length of survival, outcome etc., with chromosomal abnormalities during long-term follow-up of individual patient might explain practical significance of chromosomal changes during the course of disease.
CHROMOSOME STUDIES ON 45 PRIMARY BREAST CARCINOMAS. L. Romitti*, S.G. Carinelli** *LABORATORIO DI CITOGENETICA and **LABORATORIO DI ISTOLOGIA ED ANATOMIA PATOLOGICA, ISTITUTI CLINICI DI PERFEZIONAMENTO,MILANO Cytogenetic analysis was performed on 45 primary breast cancer, 75% of which was represented by infiltrating ductal carcinomas. In order to obtain good metaphase quality, tissue cultures were set up. Chromosome rearrangements and aneuploidies were detected in 10 cases whereas the remaining cases showed a normal diploid karyotype. No recurrent changes were identified and the presence of gene amplification was observed in 6 cases as homogeneous staining regions while no double minutes were detected. Although some authors claimed that a relevant fraction of breast cancers appears to be composed predominantly of diploid cell on the basis of flow cytometry and cytogenetic studies, the detection of a normal karyotype could be related to the desmoplasia, excessive connective tissue stroma, associated with about 75% of breast carcinomas.