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A cytogentic study of brain tumors with a major oligodendroglial component.
Abstracts
251
67 A CYTOGENETIC STUDY ( ) 1 : 6 8 Ctt[IA)tlOOD AND ADULT BRAIN TUHOIJt~,S ADAi*I L . R . + ,
EDWARI)S J . D . +,
1)AVISON E . V . +,
JAROS E . * ,
t'ERRY. ':¢ a n d PEARSON A . I ) . J .
+ DIVISION ()lv HII~IAN GENF/F[C,%, UNIVERSITY OF NEWCASTI,E IIPON TYNE ':-" I)EPARTblENT (;F ~JE[JROPATtlOI,()GY, NEIqCASTI,E GENERAl, IIOSP1TAL I)EPARTNENT OF CII[LD tlEALTll, HEDICAI, SCHOOL, UNIVERSITY OF NEWCASTLE ABSTRACT Betweeu January 1986 a n d Octcd~er 1989 w e h a v e p e r f o r m e d c y t o g e n e t i c analyses (m 68 f r e s h i n t r a c f n n i a l turnouts from children and adtlJts in t h e Nortln E a s t o f E n ? l a n d . T h e a i m of t h i s study w a s to c o r r e l a t e information on a g e , s e x , t u m o t l r h i s t o l o g y and prognoMs with the tumo.r kary(;type, in t o t a l , 57 t u r n o u t s w e r e s u c c e s s f u l l y karyotyped (8aZ). Of 3U a d u l t turnouts, 18 h a d a n abi~ormal k a r y o t y t l e (60~), while only /~ o f t h e 27 r h i l d h o o d turnouts (15~) showed any karyotypic abnormality. The m o s t f r e q u e n t 1 y o b s e r v e d abnorma 1 i t y i n this s e t i e s was monosomy 22 in t h e m e . i u g t o m a s , fotlowed I)v s e x c h r o m o s o m e a n e u p l o i d y .in the glioma group. The high proportion of- n o r m a l k a r y o t y p e s in the series o f paed[al:ric t u r n o u t s (8370) w i l l a l s o be d i s c u s s e d .
68
CYTOGENETIC STUDY OF GRADE I I I
AND IV HUMAN ASTROCYTOMAS.
S. BERTRAND, M.J. NGUYEN, A.BURONFOSSE, C. GINESTET, F. MORNEX and J.F. DORE. INSERM U.218, Centre L6on B6rard, 28 rue La~nnec, 69373 Lyon Cedex 08, France. Cytogenetic analyses were performed in 13 cases (7 males and 6 females) of primary brain tumors, h i s t o l o g i c a l l y determined as grade I I I or IV astrocytomas. 11 tumors y i e l d e d analyzable metaphases a f t e r short term t i s s u e c u l t u r e (primary c u l t u r e or low in v i t r o passage). Permanent c e l l l i n e s have been s u c c e s s f u l l y e s t a b l i s e d in monolayer c u l t u r e ; in v i t r o progression was studied. 8 tumors showed near d i p l o i d chromosome numbers (42-49 chromosomes per c e l l ) , whereas t r i - t e t r a p l o i d chromosome complements were present in 2 tumors (71-85 chromosomes per c e l l ) , a bimodal d i s t r i b u t i o n was observed in one case (45-46 and 85-89 chromosomes per c e l l ) . Karyotypes of 3 tumors were a p p a r e n t l y normal. Chromosomal changes implied numerical d e v i a t i o n s by a gain of chromosome #7 and by looses of #9, #I0 and Y. Although no common chromosomal rearrangement seems to e x i s t , chromosomes # I , #6 were predominantly i n v o l v e d . Double minutes were observed in 7 cases (2,6% to 83% of c e l l s contained DM); each c e l l contained one DM to more than hundred DM. 3 tumors of the near d i p l o i d cases showed DMs and they had p o l y p l o i d clone, the bimodal tumor had 83% of c e l l s which contained DM. Chromosomal composition may be useful in determining stage of progression and in p r e d i c t i n g prognosis or responsiveness to s p e c i f i c t h e r a peutic agents. More e s p e c i a l l y we examined r a d i a t i o n s e n s i t i v i t i e s of c e l l s in v i t r o in order to determine whether chromosomes play any r o l e in inherent r a d i o s e n s i t i v i t y . P r e l i m i n a r y r e s u l t s tend to show an increase of the number of c e l l s which contained DM f o l l o w i n g i r r a d i a t i o n . E l u c i d a t i o n of the gene(s) a m p l i f i e d in DMs w i l l be of i n t e r e s t . Nevertheless, the molecular meaning of most of chromosomal abnormalities is not well known y e t .
251
67 A CYTOGENETIC STUDY ( ) 1 : 6 8 Ctt[IA)tlOOD AND ADULT BRAIN TUHOIJt~,S ADAi*I L . R . + ,
EDWARI)S J . D . +,
1)AVISON E . V . +,
JAROS E . * ,
t'ERRY. ':¢ a n d PEARSON A . I ) . J .
+ DIVISION ()lv HII~IAN GENF/F[C,%, UNIVERSITY OF NEWCASTI,E IIPON TYNE ':-" I)EPARTblENT (;F ~JE[JROPATtlOI,()GY, NEIqCASTI,E GENERAl, IIOSP1TAL I)EPARTNENT OF CII[LD tlEALTll, HEDICAI, SCHOOL, UNIVERSITY OF NEWCASTLE ABSTRACT Betweeu January 1986 a n d Octcd~er 1989 w e h a v e p e r f o r m e d c y t o g e n e t i c analyses (m 68 f r e s h i n t r a c f n n i a l turnouts from children and adtlJts in t h e Nortln E a s t o f E n ? l a n d . T h e a i m of t h i s study w a s to c o r r e l a t e information on a g e , s e x , t u m o t l r h i s t o l o g y and prognoMs with the tumo.r kary(;type, in t o t a l , 57 t u r n o u t s w e r e s u c c e s s f u l l y karyotyped (8aZ). Of 3U a d u l t turnouts, 18 h a d a n abi~ormal k a r y o t y t l e (60~), while only /~ o f t h e 27 r h i l d h o o d turnouts (15~) showed any karyotypic abnormality. The m o s t f r e q u e n t 1 y o b s e r v e d abnorma 1 i t y i n this s e t i e s was monosomy 22 in t h e m e . i u g t o m a s , fotlowed I)v s e x c h r o m o s o m e a n e u p l o i d y .in the glioma group. The high proportion of- n o r m a l k a r y o t y p e s in the series o f paed[al:ric t u r n o u t s (8370) w i l l a l s o be d i s c u s s e d .
68
CYTOGENETIC STUDY OF GRADE I I I
AND IV HUMAN ASTROCYTOMAS.
S. BERTRAND, M.J. NGUYEN, A.BURONFOSSE, C. GINESTET, F. MORNEX and J.F. DORE. INSERM U.218, Centre L6on B6rard, 28 rue La~nnec, 69373 Lyon Cedex 08, France. Cytogenetic analyses were performed in 13 cases (7 males and 6 females) of primary brain tumors, h i s t o l o g i c a l l y determined as grade I I I or IV astrocytomas. 11 tumors y i e l d e d analyzable metaphases a f t e r short term t i s s u e c u l t u r e (primary c u l t u r e or low in v i t r o passage). Permanent c e l l l i n e s have been s u c c e s s f u l l y e s t a b l i s e d in monolayer c u l t u r e ; in v i t r o progression was studied. 8 tumors showed near d i p l o i d chromosome numbers (42-49 chromosomes per c e l l ) , whereas t r i - t e t r a p l o i d chromosome complements were present in 2 tumors (71-85 chromosomes per c e l l ) , a bimodal d i s t r i b u t i o n was observed in one case (45-46 and 85-89 chromosomes per c e l l ) . Karyotypes of 3 tumors were a p p a r e n t l y normal. Chromosomal changes implied numerical d e v i a t i o n s by a gain of chromosome #7 and by looses of #9, #I0 and Y. Although no common chromosomal rearrangement seems to e x i s t , chromosomes # I , #6 were predominantly i n v o l v e d . Double minutes were observed in 7 cases (2,6% to 83% of c e l l s contained DM); each c e l l contained one DM to more than hundred DM. 3 tumors of the near d i p l o i d cases showed DMs and they had p o l y p l o i d clone, the bimodal tumor had 83% of c e l l s which contained DM. Chromosomal composition may be useful in determining stage of progression and in p r e d i c t i n g prognosis or responsiveness to s p e c i f i c t h e r a peutic agents. More e s p e c i a l l y we examined r a d i a t i o n s e n s i t i v i t i e s of c e l l s in v i t r o in order to determine whether chromosomes play any r o l e in inherent r a d i o s e n s i t i v i t y . P r e l i m i n a r y r e s u l t s tend to show an increase of the number of c e l l s which contained DM f o l l o w i n g i r r a d i a t i o n . E l u c i d a t i o n of the gene(s) a m p l i f i e d in DMs w i l l be of i n t e r e s t . Nevertheless, the molecular meaning of most of chromosomal abnormalities is not well known y e t .