- Email: [email protected]
A marked high response of serum gastrin concentrations to the test meal may contribute to the pathogenesis of duodenal ulcer
GASTROENTEROLOGYVol. 114, No. 4
A166 AGAABSTRACTS G0677
G0679
A MARKED HIGH RESPONSE OF SERUM GASTRIN CONCENTRATIONS TO THE TEST MEAL MAY CONTRIBUTE TO THE PATHOGENESIS OF DUODENAL ULCER. T. Kamada. K. Haruma, Y. Kitadai, H. Hamada, N. Hattori, S. Kido, T. Goto, M. Mihara, K. Komoto, S. Tanaka, M. Yoshihara, K. Sumii, G. Kajiyama. First Dept. of Internal Medicine, Hiroshima University School of Medicine, Hiroshima, Japan.
THE ASSOCIATION BETWEEN HELICOBACTER PYLORI INFECTION AND CORONARY HEART DISEASE ASSESSED BY CORONARY ANGIOGRAPHY. D.H. Kang, H.S. Yoon, D.K. Park, S.S. Kim, J.J. Park, Y.K. Kim, D.J. Choi, H.C. Park, J.H. Kim, I.S. Choi, I.K. Shin, Department of Internal Medicine, Chung-Ang Gil Hospital, Inchon, Korea
Recent studies have indicated that Helicobacter pylori (17-1.py!ori) infection is associated with duodenal ulcer (DU). However, most of H. pylori-infected subjects do not develop DU. The AIM of this study was to clarify the pathogenesis of DU though investigating the meal-stimulated serum gastrin response in asymptomatic volunteers with or without tl. pylori infection and DU subjects. S_UBJECTS AND METHODS: The study population consisted of 31 H. pylori negative volunteers (27 males, mean age 26 yrs), 10 H. pylori positive volunteers (6 males, mean age 29 yrs), and 15 subjects with DU (13 males, mean age 29 yrs). After an overnight fast, blood samples were taken before and 15, 30, and 60 min after eating the test meal, which consisted of 100g rice, 130g chicken, and 1 egg (374 Kcal, 37.5 g carbohydrate, 41.5 g protein, and 11.6 g fat). The H. pylori infection status was determined by histology, serology, and the 13C-urea breath test. Serum gastrin concentrations were measured by radioimmunoassay. RESULTS: Results were expressed as the mean -+ standard error.
disease(CHD). A i m s : The aims of this study were 1) to determine the seroprevalence of H. pylori infection in patients with suspected CHD with normal and abnormal coronary angiogram and 2)to evaluate the role of H. pylori infection as a risk factor for CHD indpendent from well-known ones. M e t h o d s : 274 patients undergoing coronary angiography for suspected coronary artery disease were prospectively studied. Patients with previous history of peptic ulcer disease and digestive symptoms were excluded. Information was acquired on the presence of conventional risk factors(smoking, hyperlipidemia, diabetes, and arteial hypertension) Angiograms were read by experienced invasive cardiologists blinded to the results of H. pylori serology, which was determined by an ELISA assay. Patients with normal coronary arteries (group I) were compared to patients with occlusive coronary artery disease and subgroup analysis was performed in patients with < 50% stenosis (group II) and > 50% stenosis of at least one vessel (group III). Results: 1) 262 patients were studied(mean age 59.8, age range: 28 - 86 years, 123 females). 168(64.1%) patients were H. pylori positive. 2) 60(22.9%) patients were group I, and 42(16.0%) patients were group II and 160(61.1%) patients were group III. 63.3% of patients in group I were H. pylori positive compared with 61.9% in group II, and 65.0% in group llI(p > 0.05 by Mantel-Hanszel chi-square test). 3) H. pylori infection rates were similar in patients with normal and abnormal coronary arteries, one, two, Or three vessel disease(p > 0.05 by Mantel-Hanszel chi-square test). 4) No association was observed between H. pylori infection and different coventional risk factors(p > 0.05 by chi-square and t-test). Conclusion: The present data show that there is no significant link between CHD and H. pylori infection in Korea.
ill
.
H Duodenal ulcer e---e H, py/ori-positive subjects o - - o H. pylori-negative subjects
,-~ 200 "=
0
15
30 60 Time(rain.) ** p<0.01 as compared with H.pylori-negative subjects. p<0.01 as compared WithH pylori.positivesubjects. "t ix 0.05 as Compared with/Lpylori-positivesubjects.
CONCLUSIONS: A marked high response of serum gastrin concentrations to the test meal is observed in DU subjects and this may contribute to the pathngenesis of DU. G0678 ADRENOMEDULLIN PROTECTS GASTRIC MUCOSA BY DILATING GASTRIC MUCOSAL ARTERIOLES IN RATS. K. Kanamoto, K. Doi, K. Kawakubo, K. Aoyagi, C. Yamamoto, Y. Yano, T. Nagao, T. Kitazono, S. Ibayashi, and M. Fujishima. 2nd Dept. of Int. Med., Fac. of Med., Kyushu Univ., Fukuoka, Japan. Adrenomedullin (ADM), which shares sequence homology with CGRP, is a potent vasorelaxing peptide present in gastrointestinal tract. Effects of ADM on gastric microcirculation and gastric protection are still unclear. Aims: The present experiments were performed to elucidate whether ADM, like CGRP, has a protective effect against ulcerogenic insults. M e t h o d s : Fasted male Wistar rats anesthetized with urethane (1.25 g/kg) were used. (study I) Gastric mucosal lesions were produced by intragastric application of 70% ethanol (5 ml/kg), and ulcer index was determined after lhr. Effects of arterial infusion of ADM (25 pmol/min, via left gastric artery) were tested. (study II) Changes in diameters of gastric mucosal microvessels induced by topical application of ADM and CGRP were measured using intravital microscopy. Results:
Background:
(Z) Ulcer inde0( (% gastric mucosal injury) (zz) % Changes in diameter
°Ira i
"#00"4........ ~t2M-.V............. .....L " -7 ~"
raft °, ot.. _ . .
vehicle i.a.
ADM i.a.
vehicle-lO
H. pylori infection has been reported to increase the risk of coronary heart
G0680
THE ASSOCIATION BETWEEN HELICOBACTER INFECTION AND TRANSAMINASE. D.H. Kang, D.K. Park, H.S. Yoon, S.S. Kim, J.J. Park, Y.K. Kim, D.J. Choi, H.C. Park, J.H. Kim. Department of Internal Medicine, Chung-Ang Gil Hospital, Inchon, Korea
11. pylori, the main etiological factor for gastritis and peptic ulcer, has recently been associated with various extra-digestive disease. It is suggested that H. pylori produces granulating toxin, in addition to the vacuolating toxin called VacA which is mostly expressed together with the cytotoxin-associated protein CagA. However no association has been made between H. pylori infection and liver damage. Aim: The aim of this study was to evaluate the association between H. pylori infection and transaminase in healthy adults. Methods: Levels of biochemical laboratory tests including trasnsaminases and ELISA assay for 1-1.pylori infection were tested in serum sample of 3,488 healthy adults who resided in Inchon. All subjects who had history of liver and muscular disease were excluded and were negative for markers for viral hepatitis B and C. Levels of laboratory tests such as sGOT, sGPT, total cholesterol, triglyceride, glucose, uric acid, T3, T4, and TSH were compared between H. pylori infected and non-infected subjects. Statistic analysis was done by student t-test. Result: 1) 3,274 subjects(age range 1868, mean 46) were recruited and 2,286(69.8%) were positive for H. pylori. 2) Mean age were 44.4 ± 10.2 in 988 H. pylori negative subjects(male:606, female:382) and 46.2 ± 9.8 in 2,286 H. pylori positive subjects(male:l,570, female:716). 3) Mean levels(M ± SD) of sGPT was 23.6 ± 13.6 in H. pylori non-infected group and 25.3 ± 14.3 in 1-1.pylori infected group(p=0.0032). 4) Mean levels of total cholesterol was 188.8 ± 35.4 in H. pylori non-infected group and 192.3 -4-38.1 in infected group(p=0.006) and mean levels of uric acid was 4.8 -+ 1.4 in H. pylori non-infected group and 4.9 ± 1.4 in infected group(p=0.008). Conclusion: Serum glutamic pyruvic transaminase, total cholesterol and uric acid are increased in H. pylori infected healthy adults.
Backgrounds:
G0681 .
.
. . .
-9 -g -7 -6 -5 concentration (lOg'MI
(I) Gastric mucosal lesions occupied 36.7 ± 2.7% (Mean ± SEM) of the glandular area in the control rats. The affected area decreased to 21.8 _ 4.1% in rats treated with ADM. Arterial infusion of ADM significantly inhibited ethanol-induced mucosal injury by 41%. (II) ADM dilated gastric mucosal arterioles in a dose-dependent manner, but did not affect venules. The vasodilatory action of ADM was 10 times less potent than that of CGRP in 50% arteriolar dilation. C o n c l u s i o n s : ADM affords gastric mucosal protection against ethanolinduced injury possibly by dilating gastric mucosal arterioles in rats.
CYTOMETRIC ANALYSIS OF COLA-INDUCED GASTRIC MUCOSAL CELL PROLIFERATION IN RATS. Kapicio~lu S, Baki AH, Tekelio~lu Y, Reis AK, Arslan M, Sari M, Black Sea Technical University, School of Medicine, Dept. of Internal Medicine, Section of Gastroenterology, Dept. of Pathology. Trabzon-TURKEY Acidic beverages may affect the regulation of cell proliferation in the gastric mucosa. Therefore, we analyzed the influence of Coca Cola on gastric mucosa. Thirty rats were used for the experiment. They were anesthetized by pentobarbital 50 (mg/kg). A cannula was placed in the trachea of the anesthetized rat, a tube was inserted into the gastric lumen through the osephagus for intraluminal perfusion and another tube was inserted transduodenally in antrum to collect the aspirate. Femoral vein was cannulated for i.v. infusion. Thirty rats were divided into 3 groups, ten in each. The animals received 0,9% saline i.v. at 1 ml/h, with a simultaneous intragastric (i.g.) saline 8 ml/h for 3 h in group 1, i.v. saline and i.g. 0,15 N HCI (pH.0,9) in group 2, i.v. saline and i.g. Coca Cola (pH:2,6) in group 3.
A166 AGAABSTRACTS G0677
G0679
A MARKED HIGH RESPONSE OF SERUM GASTRIN CONCENTRATIONS TO THE TEST MEAL MAY CONTRIBUTE TO THE PATHOGENESIS OF DUODENAL ULCER. T. Kamada. K. Haruma, Y. Kitadai, H. Hamada, N. Hattori, S. Kido, T. Goto, M. Mihara, K. Komoto, S. Tanaka, M. Yoshihara, K. Sumii, G. Kajiyama. First Dept. of Internal Medicine, Hiroshima University School of Medicine, Hiroshima, Japan.
THE ASSOCIATION BETWEEN HELICOBACTER PYLORI INFECTION AND CORONARY HEART DISEASE ASSESSED BY CORONARY ANGIOGRAPHY. D.H. Kang, H.S. Yoon, D.K. Park, S.S. Kim, J.J. Park, Y.K. Kim, D.J. Choi, H.C. Park, J.H. Kim, I.S. Choi, I.K. Shin, Department of Internal Medicine, Chung-Ang Gil Hospital, Inchon, Korea
Recent studies have indicated that Helicobacter pylori (17-1.py!ori) infection is associated with duodenal ulcer (DU). However, most of H. pylori-infected subjects do not develop DU. The AIM of this study was to clarify the pathogenesis of DU though investigating the meal-stimulated serum gastrin response in asymptomatic volunteers with or without tl. pylori infection and DU subjects. S_UBJECTS AND METHODS: The study population consisted of 31 H. pylori negative volunteers (27 males, mean age 26 yrs), 10 H. pylori positive volunteers (6 males, mean age 29 yrs), and 15 subjects with DU (13 males, mean age 29 yrs). After an overnight fast, blood samples were taken before and 15, 30, and 60 min after eating the test meal, which consisted of 100g rice, 130g chicken, and 1 egg (374 Kcal, 37.5 g carbohydrate, 41.5 g protein, and 11.6 g fat). The H. pylori infection status was determined by histology, serology, and the 13C-urea breath test. Serum gastrin concentrations were measured by radioimmunoassay. RESULTS: Results were expressed as the mean -+ standard error.
disease(CHD). A i m s : The aims of this study were 1) to determine the seroprevalence of H. pylori infection in patients with suspected CHD with normal and abnormal coronary angiogram and 2)to evaluate the role of H. pylori infection as a risk factor for CHD indpendent from well-known ones. M e t h o d s : 274 patients undergoing coronary angiography for suspected coronary artery disease were prospectively studied. Patients with previous history of peptic ulcer disease and digestive symptoms were excluded. Information was acquired on the presence of conventional risk factors(smoking, hyperlipidemia, diabetes, and arteial hypertension) Angiograms were read by experienced invasive cardiologists blinded to the results of H. pylori serology, which was determined by an ELISA assay. Patients with normal coronary arteries (group I) were compared to patients with occlusive coronary artery disease and subgroup analysis was performed in patients with < 50% stenosis (group II) and > 50% stenosis of at least one vessel (group III). Results: 1) 262 patients were studied(mean age 59.8, age range: 28 - 86 years, 123 females). 168(64.1%) patients were H. pylori positive. 2) 60(22.9%) patients were group I, and 42(16.0%) patients were group II and 160(61.1%) patients were group III. 63.3% of patients in group I were H. pylori positive compared with 61.9% in group II, and 65.0% in group llI(p > 0.05 by Mantel-Hanszel chi-square test). 3) H. pylori infection rates were similar in patients with normal and abnormal coronary arteries, one, two, Or three vessel disease(p > 0.05 by Mantel-Hanszel chi-square test). 4) No association was observed between H. pylori infection and different coventional risk factors(p > 0.05 by chi-square and t-test). Conclusion: The present data show that there is no significant link between CHD and H. pylori infection in Korea.
ill
.
H Duodenal ulcer e---e H, py/ori-positive subjects o - - o H. pylori-negative subjects
,-~ 200 "=
0
15
30 60 Time(rain.) ** p<0.01 as compared with H.pylori-negative subjects. p<0.01 as compared WithH pylori.positivesubjects. "t ix 0.05 as Compared with/Lpylori-positivesubjects.
CONCLUSIONS: A marked high response of serum gastrin concentrations to the test meal is observed in DU subjects and this may contribute to the pathngenesis of DU. G0678 ADRENOMEDULLIN PROTECTS GASTRIC MUCOSA BY DILATING GASTRIC MUCOSAL ARTERIOLES IN RATS. K. Kanamoto, K. Doi, K. Kawakubo, K. Aoyagi, C. Yamamoto, Y. Yano, T. Nagao, T. Kitazono, S. Ibayashi, and M. Fujishima. 2nd Dept. of Int. Med., Fac. of Med., Kyushu Univ., Fukuoka, Japan. Adrenomedullin (ADM), which shares sequence homology with CGRP, is a potent vasorelaxing peptide present in gastrointestinal tract. Effects of ADM on gastric microcirculation and gastric protection are still unclear. Aims: The present experiments were performed to elucidate whether ADM, like CGRP, has a protective effect against ulcerogenic insults. M e t h o d s : Fasted male Wistar rats anesthetized with urethane (1.25 g/kg) were used. (study I) Gastric mucosal lesions were produced by intragastric application of 70% ethanol (5 ml/kg), and ulcer index was determined after lhr. Effects of arterial infusion of ADM (25 pmol/min, via left gastric artery) were tested. (study II) Changes in diameters of gastric mucosal microvessels induced by topical application of ADM and CGRP were measured using intravital microscopy. Results:
Background:
(Z) Ulcer inde0( (% gastric mucosal injury) (zz) % Changes in diameter
°Ira i
"#00"4........ ~t2M-.V............. .....L " -7 ~"
raft °, ot.. _ . .
vehicle i.a.
ADM i.a.
vehicle-lO
H. pylori infection has been reported to increase the risk of coronary heart
G0680
THE ASSOCIATION BETWEEN HELICOBACTER INFECTION AND TRANSAMINASE. D.H. Kang, D.K. Park, H.S. Yoon, S.S. Kim, J.J. Park, Y.K. Kim, D.J. Choi, H.C. Park, J.H. Kim. Department of Internal Medicine, Chung-Ang Gil Hospital, Inchon, Korea
11. pylori, the main etiological factor for gastritis and peptic ulcer, has recently been associated with various extra-digestive disease. It is suggested that H. pylori produces granulating toxin, in addition to the vacuolating toxin called VacA which is mostly expressed together with the cytotoxin-associated protein CagA. However no association has been made between H. pylori infection and liver damage. Aim: The aim of this study was to evaluate the association between H. pylori infection and transaminase in healthy adults. Methods: Levels of biochemical laboratory tests including trasnsaminases and ELISA assay for 1-1.pylori infection were tested in serum sample of 3,488 healthy adults who resided in Inchon. All subjects who had history of liver and muscular disease were excluded and were negative for markers for viral hepatitis B and C. Levels of laboratory tests such as sGOT, sGPT, total cholesterol, triglyceride, glucose, uric acid, T3, T4, and TSH were compared between H. pylori infected and non-infected subjects. Statistic analysis was done by student t-test. Result: 1) 3,274 subjects(age range 1868, mean 46) were recruited and 2,286(69.8%) were positive for H. pylori. 2) Mean age were 44.4 ± 10.2 in 988 H. pylori negative subjects(male:606, female:382) and 46.2 ± 9.8 in 2,286 H. pylori positive subjects(male:l,570, female:716). 3) Mean levels(M ± SD) of sGPT was 23.6 ± 13.6 in H. pylori non-infected group and 25.3 ± 14.3 in 1-1.pylori infected group(p=0.0032). 4) Mean levels of total cholesterol was 188.8 ± 35.4 in H. pylori non-infected group and 192.3 -4-38.1 in infected group(p=0.006) and mean levels of uric acid was 4.8 -+ 1.4 in H. pylori non-infected group and 4.9 ± 1.4 in infected group(p=0.008). Conclusion: Serum glutamic pyruvic transaminase, total cholesterol and uric acid are increased in H. pylori infected healthy adults.
Backgrounds:
G0681 .
.
. . .
-9 -g -7 -6 -5 concentration (lOg'MI
(I) Gastric mucosal lesions occupied 36.7 ± 2.7% (Mean ± SEM) of the glandular area in the control rats. The affected area decreased to 21.8 _ 4.1% in rats treated with ADM. Arterial infusion of ADM significantly inhibited ethanol-induced mucosal injury by 41%. (II) ADM dilated gastric mucosal arterioles in a dose-dependent manner, but did not affect venules. The vasodilatory action of ADM was 10 times less potent than that of CGRP in 50% arteriolar dilation. C o n c l u s i o n s : ADM affords gastric mucosal protection against ethanolinduced injury possibly by dilating gastric mucosal arterioles in rats.
CYTOMETRIC ANALYSIS OF COLA-INDUCED GASTRIC MUCOSAL CELL PROLIFERATION IN RATS. Kapicio~lu S, Baki AH, Tekelio~lu Y, Reis AK, Arslan M, Sari M, Black Sea Technical University, School of Medicine, Dept. of Internal Medicine, Section of Gastroenterology, Dept. of Pathology. Trabzon-TURKEY Acidic beverages may affect the regulation of cell proliferation in the gastric mucosa. Therefore, we analyzed the influence of Coca Cola on gastric mucosa. Thirty rats were used for the experiment. They were anesthetized by pentobarbital 50 (mg/kg). A cannula was placed in the trachea of the anesthetized rat, a tube was inserted into the gastric lumen through the osephagus for intraluminal perfusion and another tube was inserted transduodenally in antrum to collect the aspirate. Femoral vein was cannulated for i.v. infusion. Thirty rats were divided into 3 groups, ten in each. The animals received 0,9% saline i.v. at 1 ml/h, with a simultaneous intragastric (i.g.) saline 8 ml/h for 3 h in group 1, i.v. saline and i.g. 0,15 N HCI (pH.0,9) in group 2, i.v. saline and i.g. Coca Cola (pH:2,6) in group 3.