A new antiserum with conformational specificity for LHRH: Usefulness for radioimmunoassay and immunocytochemistry

Peptides, Vol. 6, p. 353, 1985. ©Ankho International Inc. Printed in the U.S.A.

0196-9781/85 $3.00 + .00

ERRATUM

JANUARY~FEBRUARY

ISSUE

ELLINWOOD, W. E., O, K. RONNEKLEIV, M. J. KELLY AND J. A. RESKO. A new antiserum with conformational specificity for LHRH: Usefulness for radioimmunoassay and immunocytochemistry. PEPTIDES 6(1) 45-52, 1985.

T h e a u t h o r s w i s h to m a k e the following c o r r e c t i o n . C o m p o u n d n u m b e r 14 in Table 1 (page 47) s h o u l d r e a d as s h o w n below:

TABLE 1 SPECIFICITY OF ANTISERUM EL-14 IN LHRH RADIOIMMUNOASSAY

No.

Relative activity (%)t

Compound*

1. pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 (LHRH) 2. pGlu ..................................................COOH 3. His ..........................................GIy-NH2 4. Trp ...................................GIy-NHz 5. Ser- .............................GIy-NHz 6. Tyr- ........................GIy-NH2 7. Gly ................... GIy-NHz

8. Leu ............GIy-NH2 9. Leu-Arg-Pro-OH 10. pGlu ...................... GIy-OH I I. pGlu-His-OH 12. pGlu-dHis ........................................ GIy-NH2 13. pGlu ..................................... Leu .....GIy-NH~ 14. pGlu ( ) ........................................... GIy-NH2 15. pGlu-- .............................. Trp-Leu .....GIy-NH2 (salmon LHRH) 16. p-Aminophenyl-AlaGlu ................................................. GIy-NH2 17. Thyrotropin Releasing Hormone (TRH) 18. Oxytocin 19. Arg-Vasopressin 20. Substance P 21. Neurotensin 22. fl-Endorphin 23. Met-Enkephalin 24. Leu-Enkephalin 25. a-Melanocyte Stimulating Hormone (a-MSH)

100.00 <0.01 0.52 0.67 <0.01 0.03 <0.01 <0.01 <0.01 <0.01 <0.01 44.61 0.78 0.26 18.13 2.00 <0.01 <0.01 <0.01 <0.01 <0.01 0.07 0.03 <0.01 <0.01

*Sources of peptides: I, 4, 5, Vega Biochemicals, Tucson, AZ; 16, 18-21, 24, 25, Bachem, Torrance, CA; 22, 23, Beckman Instruments, Inc., Palo Alto, CA; 17, Calbiochem~Behring Corp., La Jolla, CA; 2, 3, 6-14, Dr. J. Sandow, Hoechst AG, Frankfurt, West Germany; 15, Data courtesy of Dr. Nancy Sherwood, University of Victoria, Victoria, B.C., Canada. pMoles LHRH at 0.5 B/Bo tRelative activity -

pMoles test peptide at 0.5 B/Bo

353

x 100