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A new approach to assessment of severity of carotid atherosclerosis on trace element composition in hair
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Abstracts / Atherosclerosis 241 (2015) e72ee148
EAS-0125. BIOMARKERS OF ATHEROSCLEROTIC PLAQUES INSTABILITY. RESULTS OF ARTERIAL WALL AND BLOOD STUDIES Y. Ragino 1, *, A. Chernjavskiy 2, Y.A.N.A. Polonskaya 1, O. Kamenskaja 2, E. Kashtanova 1, M. Voevoda 3. 1 Laboratory of Biochemistry, Federal State Budgetary Institution of Internal and Preventive Medicine Siberian, Novosibirsk, Russia; 2 Laboratory of Cardiology, Research State of Circulation Pathology Ministry of Public Health and Social Development, Novosibirsk, Russia; 3 Laboratory of Genetics, Federal State Budgetary Institution of Internal and Preventive Medicine Siberian, Novosibirsk, Russia
und their relations) with optical emission spectrometry with inductively coupled argon plasma on Optima-2400DV (USA). Then, using a set of data on extreme values define the functional we calculated the index of severity of atherosclerosis. Index values from 0 to 0.33 indicate the initial degree of atherosclerosis (positive predictive value was 78%). The index values from 0.33 to 0.66 indicate moderate degree of atherosclerosis (positive predictive value was 83%).The index values from of 0.66 and above-show severe atherosclerosis development (positive predictive value was 85%). This index reflects the atherogenic potential of subject on content of trace elements in hair, and allows us to stratify patients at high risk for early development of atherosclerosis individually for each patient.
* Corresponding author. The aim of the study was to revealed the key significant inflammatorydestructive, oxidative and endothelial dysfunction biomarkers of coronary atherosclerotic plaques instability and investigate of their blood levels in men with coronary atherosclerosis (CA). Concentrations of inflammatory-destructive (tumor necrotic factor, TNF-alpha, interleukins, IL-1-beta and its receptor antagonist, IL-6, IL8, IL-18, IL-2, ligand of CD40 receptor (sCD40L), high sensitive Creactive protein (hsCRP), monocyte chemotactic protein -1, endothelial monocyte activating protein II, adhesive molecules (sICAM-1 and sVCAM-1), matrix metalloproteinase (MMP-3, MMP-7, MMP-9), tissue inhibitor of metalloproteinase (TIMP-1) and endothelin-1), oxidative and endothelial dysfunction (concentrations of lipid peroxidation products, LPO, including in low density lipoproteins (LDL), proteins oxidative modification, paraoxonase activity, antioxidants concentrations, lipid parameters) and endothelial dysfunction biomarkers were studied in blood and in atherosclerotic plaques of coronary artery in men with CA. Blood levels of hsCRP, IL-8, IL-6, sCD40L, oxidized LDL apolipoproteins and lipoprotein (a) were higher, but blood levels of sVCAM, TIMP-1, NO metabolites and resistance of LDL to oxidation were lower in men with prevalence of unstable atherosclerotic plaques in coronary arteries compared to men with prevalence of stable atherosclerotic plaques in coronary arteries. Blood levels of hsCRP, IL-6, IL-8, oxidized proteins, NO metabolites and sVCAM were correlated with coronary artery atherosclerotic plaques instability. Correlation of hsCRP blood level with hsCRP content in coronary artery atherosclerotic plaques and correlation of blood LPO parameters with LPO content in coronary artery atherosclerotic plaques were revealed. The study was supported by Grant RFBI 09-04-00374 and Grant of Ministry of Science of the Russia 14.740.11.0174.
EAS-0223. A NEW APPROACH TO ASSESSMENT OF SEVERITY OF CAROTID ATHEROSCLEROSIS ON TRACE ELEMENT COMPOSITION IN HAIR K. Yokubov 1, *, G. Rozikhodjaeva 1, N. Ignatiev 2, S.H. Madrahimov 2, D. Rozikhodjaeva 3. 1 Diagnostic, Central Clinical Hospital 1, Tashkent, Uzbekistan; 2 of Applied Mathematics, National university of Uzbekistan, Tashkent, Uzbekistan; 3 Information Technology, University of Information Technology, Tashkent, Uzbekistan
* Corresponding author. The object of our study was to quantify the severity and to predict the degree of atherosclerosis on individual informative features. Individual set of attributes of the subject, which we determined by its local metric allowed to calculate the index of the severity of atherosclerosis. Atherosclerosis severity index was calculated from the individual sets of informative parameters in the interval [0, 1]. We studied 150 patients with carotid atherosclerosis (CA) (mean age 64, 7 ± 7, 5 years) in Central Clinical Hospital 1 in Tashkent. In addition to all clinical examination, we conducted an additional quantification of trace element composition in hair (Fe, Cu, Al, Zn, Cd, Se, Ca, K, Na, Mg
EAS-0239. CALCIPRESSIN PLASMA LEVEL DIFFERENCE BETWEEN ATHEROSCLEROTIC AND NON-ATHEROSCLEROTIC SUBJECTS E. Torac 1, *, S.L. Magda 2, D. Vinereanu 2, L. Gaman 1, I. Stoian 1, A. Torac 3, A. Roceanu 4, C. Bleotu 5, V. Atanasiu 1. 1 Biochemistry, University of Medicine and Pharmacy “Carol Davila” Bucharest, Bucharest, Romania; 2 Cardiology, University and Emergency Hospital Bucharest, Bucharest, Romania; 3 Fundamental Research in Marketing, Bucharest University of Economic Studies, Bucharest, Romania; 4 Neurology, University and Emergency Hospital Bucharest, Bucharest, Romania; 5 Viral Genetic Engineering, Stefan S. Nicolau Institute of Virology, Bucharest, Romania
* Corresponding author. Background: Calcipressin is an inhibitor of calcineurin, located in endothelial cells, heart, brain, skeletal muscle. An abnormal regulation of calcipressin gene expression has been associated with cardiac hypertrophy, angiogenesis, tumorgenesis. As well, an up-regulated calcipressin gene expression in atheroscrelotic plaques was evaluated. The aim objective of this study was to find if the calcipressin plasma level is significantly different between atherosclerotic and non-atherosclerotic subjects. Materials and methods: Calcipressin plasma was evaluated using Human Calcipressin-RCAN1 ELISA standardized kit (Cusabio). The study enroled 28 subjects (16 atherosclerotic and 12 non-atherosclerotic). The calcipressin concentration level was evaluated on EDTA-plasma from venous blood. The atherosclerotic patients (recently diagnosed monovascular disease in coronary angiography or high intima media tickness in patients with cardiovascular risk factors) were on statin treatment for a week on average. Results: Regarding the calcipressin concentration in blood, a statistically significant difference between atherosclerotic male subjects versus nonatherosclerotic male subjects was found (p<0.05). Atherosclerotic men, on statin treatment, present a plasma calcipressin concentration lower than the non-atheroscletoric men. In case of both females samples no statistically significant difference was found.
Subjects
Calcipressin pg/ml Mean ± SEM
Atherosclerotic males Non-atherosclerotic males Atherosclerotic females Non-atherosclerotic females
222 ± 29.62 317.6 ± 31.56 208.4 ± 21.95 236 ± 26.88
Conclusion: A difference of plasma calcipressin level between atherosclerotic subjects versus non-atherosclerotic subjects was revealed. This study encourages further experimental determinations with larger sample of subjects. Acknowledgement: Ms. Elena Torac was supported by the doctoral program POSDRU/159/1.5/S/137390, from the European Social Fund.
Abstracts / Atherosclerosis 241 (2015) e72ee148
EAS-0125. BIOMARKERS OF ATHEROSCLEROTIC PLAQUES INSTABILITY. RESULTS OF ARTERIAL WALL AND BLOOD STUDIES Y. Ragino 1, *, A. Chernjavskiy 2, Y.A.N.A. Polonskaya 1, O. Kamenskaja 2, E. Kashtanova 1, M. Voevoda 3. 1 Laboratory of Biochemistry, Federal State Budgetary Institution of Internal and Preventive Medicine Siberian, Novosibirsk, Russia; 2 Laboratory of Cardiology, Research State of Circulation Pathology Ministry of Public Health and Social Development, Novosibirsk, Russia; 3 Laboratory of Genetics, Federal State Budgetary Institution of Internal and Preventive Medicine Siberian, Novosibirsk, Russia
und their relations) with optical emission spectrometry with inductively coupled argon plasma on Optima-2400DV (USA). Then, using a set of data on extreme values define the functional we calculated the index of severity of atherosclerosis. Index values from 0 to 0.33 indicate the initial degree of atherosclerosis (positive predictive value was 78%). The index values from 0.33 to 0.66 indicate moderate degree of atherosclerosis (positive predictive value was 83%).The index values from of 0.66 and above-show severe atherosclerosis development (positive predictive value was 85%). This index reflects the atherogenic potential of subject on content of trace elements in hair, and allows us to stratify patients at high risk for early development of atherosclerosis individually for each patient.
* Corresponding author. The aim of the study was to revealed the key significant inflammatorydestructive, oxidative and endothelial dysfunction biomarkers of coronary atherosclerotic plaques instability and investigate of their blood levels in men with coronary atherosclerosis (CA). Concentrations of inflammatory-destructive (tumor necrotic factor, TNF-alpha, interleukins, IL-1-beta and its receptor antagonist, IL-6, IL8, IL-18, IL-2, ligand of CD40 receptor (sCD40L), high sensitive Creactive protein (hsCRP), monocyte chemotactic protein -1, endothelial monocyte activating protein II, adhesive molecules (sICAM-1 and sVCAM-1), matrix metalloproteinase (MMP-3, MMP-7, MMP-9), tissue inhibitor of metalloproteinase (TIMP-1) and endothelin-1), oxidative and endothelial dysfunction (concentrations of lipid peroxidation products, LPO, including in low density lipoproteins (LDL), proteins oxidative modification, paraoxonase activity, antioxidants concentrations, lipid parameters) and endothelial dysfunction biomarkers were studied in blood and in atherosclerotic plaques of coronary artery in men with CA. Blood levels of hsCRP, IL-8, IL-6, sCD40L, oxidized LDL apolipoproteins and lipoprotein (a) were higher, but blood levels of sVCAM, TIMP-1, NO metabolites and resistance of LDL to oxidation were lower in men with prevalence of unstable atherosclerotic plaques in coronary arteries compared to men with prevalence of stable atherosclerotic plaques in coronary arteries. Blood levels of hsCRP, IL-6, IL-8, oxidized proteins, NO metabolites and sVCAM were correlated with coronary artery atherosclerotic plaques instability. Correlation of hsCRP blood level with hsCRP content in coronary artery atherosclerotic plaques and correlation of blood LPO parameters with LPO content in coronary artery atherosclerotic plaques were revealed. The study was supported by Grant RFBI 09-04-00374 and Grant of Ministry of Science of the Russia 14.740.11.0174.
EAS-0223. A NEW APPROACH TO ASSESSMENT OF SEVERITY OF CAROTID ATHEROSCLEROSIS ON TRACE ELEMENT COMPOSITION IN HAIR K. Yokubov 1, *, G. Rozikhodjaeva 1, N. Ignatiev 2, S.H. Madrahimov 2, D. Rozikhodjaeva 3. 1 Diagnostic, Central Clinical Hospital 1, Tashkent, Uzbekistan; 2 of Applied Mathematics, National university of Uzbekistan, Tashkent, Uzbekistan; 3 Information Technology, University of Information Technology, Tashkent, Uzbekistan
* Corresponding author. The object of our study was to quantify the severity and to predict the degree of atherosclerosis on individual informative features. Individual set of attributes of the subject, which we determined by its local metric allowed to calculate the index of the severity of atherosclerosis. Atherosclerosis severity index was calculated from the individual sets of informative parameters in the interval [0, 1]. We studied 150 patients with carotid atherosclerosis (CA) (mean age 64, 7 ± 7, 5 years) in Central Clinical Hospital 1 in Tashkent. In addition to all clinical examination, we conducted an additional quantification of trace element composition in hair (Fe, Cu, Al, Zn, Cd, Se, Ca, K, Na, Mg
EAS-0239. CALCIPRESSIN PLASMA LEVEL DIFFERENCE BETWEEN ATHEROSCLEROTIC AND NON-ATHEROSCLEROTIC SUBJECTS E. Torac 1, *, S.L. Magda 2, D. Vinereanu 2, L. Gaman 1, I. Stoian 1, A. Torac 3, A. Roceanu 4, C. Bleotu 5, V. Atanasiu 1. 1 Biochemistry, University of Medicine and Pharmacy “Carol Davila” Bucharest, Bucharest, Romania; 2 Cardiology, University and Emergency Hospital Bucharest, Bucharest, Romania; 3 Fundamental Research in Marketing, Bucharest University of Economic Studies, Bucharest, Romania; 4 Neurology, University and Emergency Hospital Bucharest, Bucharest, Romania; 5 Viral Genetic Engineering, Stefan S. Nicolau Institute of Virology, Bucharest, Romania
* Corresponding author. Background: Calcipressin is an inhibitor of calcineurin, located in endothelial cells, heart, brain, skeletal muscle. An abnormal regulation of calcipressin gene expression has been associated with cardiac hypertrophy, angiogenesis, tumorgenesis. As well, an up-regulated calcipressin gene expression in atheroscrelotic plaques was evaluated. The aim objective of this study was to find if the calcipressin plasma level is significantly different between atherosclerotic and non-atherosclerotic subjects. Materials and methods: Calcipressin plasma was evaluated using Human Calcipressin-RCAN1 ELISA standardized kit (Cusabio). The study enroled 28 subjects (16 atherosclerotic and 12 non-atherosclerotic). The calcipressin concentration level was evaluated on EDTA-plasma from venous blood. The atherosclerotic patients (recently diagnosed monovascular disease in coronary angiography or high intima media tickness in patients with cardiovascular risk factors) were on statin treatment for a week on average. Results: Regarding the calcipressin concentration in blood, a statistically significant difference between atherosclerotic male subjects versus nonatherosclerotic male subjects was found (p<0.05). Atherosclerotic men, on statin treatment, present a plasma calcipressin concentration lower than the non-atheroscletoric men. In case of both females samples no statistically significant difference was found.
Subjects
Calcipressin pg/ml Mean ± SEM
Atherosclerotic males Non-atherosclerotic males Atherosclerotic females Non-atherosclerotic females
222 ± 29.62 317.6 ± 31.56 208.4 ± 21.95 236 ± 26.88
Conclusion: A difference of plasma calcipressin level between atherosclerotic subjects versus non-atherosclerotic subjects was revealed. This study encourages further experimental determinations with larger sample of subjects. Acknowledgement: Ms. Elena Torac was supported by the doctoral program POSDRU/159/1.5/S/137390, from the European Social Fund.