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A new biosurfactant produced by soil bacterial strain and its application in bioremediation
S126
Abstracts / New Biotechnology 33S (2016) S1–S213
The rhamnolipids influence on bacterial consortia was defined with the use of spectrophotometry methods. Acknowledgement. This study was supported by NCN grant: DEC-2012/07/D/NZ9/00981. http://dx.doi.org/10.1016/j.nbt.2016.06.1158
P13-8 Screening of cyanobacterial peptide toxin, microcystins in hyperscum water samples from an inland sub Saharan drinking freshwater reservoir Kennedy Eguzozie ∗ , Vuyo Mavumgwana, Eugenie Kayitesi
and October, while bacteria were detectable in all samples. The following yeast genera/species have been identified: Crytpococcus sp., Geotrichum candidum, Hanseniaspora occidentalis, Metschnikowia fructicola, Torulaspora delbrueckii. These yeast strains are also able to colonize the surface of grape, and certain species of Hanseniaspora and Torulaspora dominate the initial stages of fermentation. We managed to detect 9 bacterial and 5 yeast genera in soil samples, including yeasts that may play a role during must fermentation. In the near future we would like to continue this project and study the dynamics of microbial population. http://dx.doi.org/10.1016/j.nbt.2016.06.1160
P13-11
University of Johannesburg A study which probed the occurrence and quantitative variations hepatotoxic microcystin in a Sub Saharan drinking freshwater reservoir was carried out between November 2014 and March 2015. Results reveal the presence of MCYSTYR, MCYST-LR, MCYST-RR, MCYST-LA and MCYST-LF variants either in cells collected directly from bloom or toxic isolates cultured under laboratory conditions. Two minor microcystin congeners (MCYST-(H4 )YR) and (D-Asp3 , Dha7 ) MCYST-RR) were identified, but not quantified. Variants dominance were in the order MCYST-LR > MCYST-RR > MCYST-YR > MCYST-LA > MCYST-LF across sampling sites. Maximum and minimum concentrations of quantified MCYSTs congeners were (489.25, 50.95 g toxin/g DW), (98.92, 9.11 g toxin/g DW), (140.25, 12.07 g toxin/g DW), (56.99, 6.20 g toxin/g DW) and (50.46, 3.65 g toxin/g DW) for MCYSTLR, MCYST-YR, MCYST-RR, MCYST-LA and MCYST-LF respectively. Analysis of variance (ANOVA) revealed there was a high significant difference between mean microcystin concentrations across sampling sites (P < 0.05). http://dx.doi.org/10.1016/j.nbt.2016.06.1159
P13-10 Analysis of the soil microbial community in the Badacsony wine region ˝ 1,∗ , Sára Pál 1 , Barna Szoke ˝ 2 , János Májer 2 , Annamária Gerocs 1 Ferenc Olasz 1 2
Agricultural Biotechnology Institute, United States Research Institute for Viticulture and Enology, United States
The physical, chemical and biological properties of the soil influence the quality of grape and wine, and contribute to the character of “terroir” wines. Our aim is to study soil microorganisms in a selected vineyard in the Badacsony wine region, with particular emphasis on yeasts. Soil samples have been collected at monthly intervals from the vineyard of NARIC-RIVE since September 2015. Different selective medium were applied for the isolation of yeasts and bacteria, and the isolated strains were categorized by molecular biology methods (PCR, PCR-RFLP). The nucleotide sequences of ribosomal DNA were used for the identification of the isolates. In order to detect viable but non-culturable fungal and bacterial strains, we extracted genomic DNA from soil samples for metagenomic analysis. Four hundred and twentythree isolates, including 417 bacteria (Acinetobacter, Aeromonas, Arthrobacter, Bacillus, Enterobacter, Escherichia, Pseudomonas, Rahnella, Serratia) and 6 yeasts, were tested by microbiological assays. Yeast isolates were only detected in samples taken in September
A new biosurfactant produced by soil bacterial strain and its application in bioremediation Agata Zdarta ∗ , Wojciech Smułek, Ewa Kaczorek Poznan University of Technology, Poland Microbial compounds, which reduce surface and interfacial tensions in both aqueous solutions and hydrocarbon mixtures are known as biosurfactants. These molecules have unique amphiphilic properties, which suggest potential applications in the environmental protection, crude oil recovery, health care, pharmaceutical and food-processing industries. In contrast to almost all surfactants currently in use, microbial surfactants have a great future ahead due to their environmentally friendly nature and the possibility of their production through fermentation. This study reports the potential of a newly isolated soil bacterial strain, to produce biosurfactant, whose composition was evaluated by chromatographic and spectrophotometric methods. The analysis of surface properties allowed the calculation of adsorption parameters and critical micelle concentration of the biosurfactant. Microbial surfactant was also tested for use in bioremediation of petroleum products, in order to increase the bioavailability of pollutants to microorganisms. The impact of the surfactant on diesel oil biodegradation by selected bacterial strains was tested. Likewise, influence of obtained substance on cell surface hydrophobicity was examined. The results suggest that the biosurfactant produced is characterized by good surface active properties and can lower the water surface tension to 31.7 mN/m. However, favorable effect of the surfactant on hydrocarbons biodegradation depends on the strain used. More information on the surface tension would help us establish to a greater degree of accuracy its application as an efficient tool in various biotechnological, industrial, and environmental applications. This study was supported by The National Science Centre awarded by decisions number DEC-2012/07/B/NZ9/00950. http://dx.doi.org/10.1016/j.nbt.2016.06.1161
P13-12 Antioxidative activity of cabbage leaves caused by soil contamination with zinc and cadmium Renata Baczek-Kwinta ∗ , Małgorzata Borek, Marcin Rapacz, Jacek Antonkiewicz University of Agriculture in Krakow, Poland
Abstracts / New Biotechnology 33S (2016) S1–S213
The rhamnolipids influence on bacterial consortia was defined with the use of spectrophotometry methods. Acknowledgement. This study was supported by NCN grant: DEC-2012/07/D/NZ9/00981. http://dx.doi.org/10.1016/j.nbt.2016.06.1158
P13-8 Screening of cyanobacterial peptide toxin, microcystins in hyperscum water samples from an inland sub Saharan drinking freshwater reservoir Kennedy Eguzozie ∗ , Vuyo Mavumgwana, Eugenie Kayitesi
and October, while bacteria were detectable in all samples. The following yeast genera/species have been identified: Crytpococcus sp., Geotrichum candidum, Hanseniaspora occidentalis, Metschnikowia fructicola, Torulaspora delbrueckii. These yeast strains are also able to colonize the surface of grape, and certain species of Hanseniaspora and Torulaspora dominate the initial stages of fermentation. We managed to detect 9 bacterial and 5 yeast genera in soil samples, including yeasts that may play a role during must fermentation. In the near future we would like to continue this project and study the dynamics of microbial population. http://dx.doi.org/10.1016/j.nbt.2016.06.1160
P13-11
University of Johannesburg A study which probed the occurrence and quantitative variations hepatotoxic microcystin in a Sub Saharan drinking freshwater reservoir was carried out between November 2014 and March 2015. Results reveal the presence of MCYSTYR, MCYST-LR, MCYST-RR, MCYST-LA and MCYST-LF variants either in cells collected directly from bloom or toxic isolates cultured under laboratory conditions. Two minor microcystin congeners (MCYST-(H4 )YR) and (D-Asp3 , Dha7 ) MCYST-RR) were identified, but not quantified. Variants dominance were in the order MCYST-LR > MCYST-RR > MCYST-YR > MCYST-LA > MCYST-LF across sampling sites. Maximum and minimum concentrations of quantified MCYSTs congeners were (489.25, 50.95 g toxin/g DW), (98.92, 9.11 g toxin/g DW), (140.25, 12.07 g toxin/g DW), (56.99, 6.20 g toxin/g DW) and (50.46, 3.65 g toxin/g DW) for MCYSTLR, MCYST-YR, MCYST-RR, MCYST-LA and MCYST-LF respectively. Analysis of variance (ANOVA) revealed there was a high significant difference between mean microcystin concentrations across sampling sites (P < 0.05). http://dx.doi.org/10.1016/j.nbt.2016.06.1159
P13-10 Analysis of the soil microbial community in the Badacsony wine region ˝ 1,∗ , Sára Pál 1 , Barna Szoke ˝ 2 , János Májer 2 , Annamária Gerocs 1 Ferenc Olasz 1 2
Agricultural Biotechnology Institute, United States Research Institute for Viticulture and Enology, United States
The physical, chemical and biological properties of the soil influence the quality of grape and wine, and contribute to the character of “terroir” wines. Our aim is to study soil microorganisms in a selected vineyard in the Badacsony wine region, with particular emphasis on yeasts. Soil samples have been collected at monthly intervals from the vineyard of NARIC-RIVE since September 2015. Different selective medium were applied for the isolation of yeasts and bacteria, and the isolated strains were categorized by molecular biology methods (PCR, PCR-RFLP). The nucleotide sequences of ribosomal DNA were used for the identification of the isolates. In order to detect viable but non-culturable fungal and bacterial strains, we extracted genomic DNA from soil samples for metagenomic analysis. Four hundred and twentythree isolates, including 417 bacteria (Acinetobacter, Aeromonas, Arthrobacter, Bacillus, Enterobacter, Escherichia, Pseudomonas, Rahnella, Serratia) and 6 yeasts, were tested by microbiological assays. Yeast isolates were only detected in samples taken in September
A new biosurfactant produced by soil bacterial strain and its application in bioremediation Agata Zdarta ∗ , Wojciech Smułek, Ewa Kaczorek Poznan University of Technology, Poland Microbial compounds, which reduce surface and interfacial tensions in both aqueous solutions and hydrocarbon mixtures are known as biosurfactants. These molecules have unique amphiphilic properties, which suggest potential applications in the environmental protection, crude oil recovery, health care, pharmaceutical and food-processing industries. In contrast to almost all surfactants currently in use, microbial surfactants have a great future ahead due to their environmentally friendly nature and the possibility of their production through fermentation. This study reports the potential of a newly isolated soil bacterial strain, to produce biosurfactant, whose composition was evaluated by chromatographic and spectrophotometric methods. The analysis of surface properties allowed the calculation of adsorption parameters and critical micelle concentration of the biosurfactant. Microbial surfactant was also tested for use in bioremediation of petroleum products, in order to increase the bioavailability of pollutants to microorganisms. The impact of the surfactant on diesel oil biodegradation by selected bacterial strains was tested. Likewise, influence of obtained substance on cell surface hydrophobicity was examined. The results suggest that the biosurfactant produced is characterized by good surface active properties and can lower the water surface tension to 31.7 mN/m. However, favorable effect of the surfactant on hydrocarbons biodegradation depends on the strain used. More information on the surface tension would help us establish to a greater degree of accuracy its application as an efficient tool in various biotechnological, industrial, and environmental applications. This study was supported by The National Science Centre awarded by decisions number DEC-2012/07/B/NZ9/00950. http://dx.doi.org/10.1016/j.nbt.2016.06.1161
P13-12 Antioxidative activity of cabbage leaves caused by soil contamination with zinc and cadmium Renata Baczek-Kwinta ∗ , Małgorzata Borek, Marcin Rapacz, Jacek Antonkiewicz University of Agriculture in Krakow, Poland