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A new method for staining paper electropherograms.
1960
Abstracts
Section
A 139
]4541 COULOMETRIC MICRO-DETERMINATION OF BROMURAL (a-BROMISOVALERYLUREA) AND CABROMAL (a-BROM-DIETHYLACETYLUREA). K.Kalinowski. (Dept. of Pharmaceutical Chemistry, Academy of Medicine, LBdf, Poland.) Acta Pharm. Polon. ~ 16 (1959) 225. Bromural (BM) and cabromal (CB) were determined by coulometric titration at constant current by electrolytically generated Ag ions. For determination of 0.1 g of BM or CB, 15 ml of water and 1 ml of 15% NaOH were added to the sample and the flask refluxed on a water bath for 15 min. When the solution had cooled 3-5 ml of 30% hydrogen peroxide was added and gently heated. This solution was acidified with acetic acid adding 6 ml of 10% solution in excess. To the electrolytic cell l/125 of this solution and 28 ml 10% KNoj were added withexclusion of light and Ag ions were generated. The current was of the order of 5 mA. The end-point was determined amperometrically with 3 different indicator electrode systems: (1) with 2 polarized Ag electrodes (200 mV); (2) with Ag and Pt electrodes, polarized with 200 mV; and (3) with a Cu-Ag electrode system without external voltage. In all the systems the results were consistent, the error being less than 1%. Ad. Hu. 14551 COULOMETRIC DETERMINATION OF CYCLOHEXAL AND HEXOBARBITONE. K.Kalinowski and H.Baran. (Dept. of Pharmaceutical Chemistry, Academy of Medicine, L,6dL, Poland.) Acta Pharm. Polon., 16 (1959) 231. acid) (CH) and hexobarbitone Cyclohexal 5 - A1 cyclohexenyl-5-ethylbarbituric acid) (HB) were determined by (N-methyl-5-A i -cyclohexenyl-5-methylbarbituric coulometric titration at constant current with electrolytically generated chlorine. For determination of CH 10 ml of about 0.02% solution of CH was mixed with 10 ml of 10% HCl. In HB determination 10 ml of 5% HCl was used. The end-point was determined by amperometry with 2 polarized Pt electrodes. The error of determination was less than 0.7%. Ad. Hu. See also: [255,272,394].
8. ELECTROPHORESIS I4561 A NEW METHOD FOR STAINING PAPER ELECTROPHEROGRAMS. wa. Biokhimiya, 24 (1959) 127.
M. N. Jegoro-
The paper is stained by means of 0.08% solution of acid-deep-blue in 10% AcOH to eliminate the excess dye; the stained bands are eluted with 0.1 N NaOH and the optical densities at 520 mu read with a calorimeter. The intensities are stable for at least 30 min. Ca. Cas. ]4571 EVALUATION OF ELECTROPHORESIS DIAGRAMS. Sorin M. Idu and Nicolae B. Cucu. Hoppe-Seyler’s Z. physiol. Chem. , 314 (1959) 284. With the aid of a simple algebraic method and of a suitably traced diagram method of Wallner Ulke for measuring areas under electrophoresis diagrams’ been simplified.
the has
Abstracts
Section
A 139
]4541 COULOMETRIC MICRO-DETERMINATION OF BROMURAL (a-BROMISOVALERYLUREA) AND CABROMAL (a-BROM-DIETHYLACETYLUREA). K.Kalinowski. (Dept. of Pharmaceutical Chemistry, Academy of Medicine, LBdf, Poland.) Acta Pharm. Polon. ~ 16 (1959) 225. Bromural (BM) and cabromal (CB) were determined by coulometric titration at constant current by electrolytically generated Ag ions. For determination of 0.1 g of BM or CB, 15 ml of water and 1 ml of 15% NaOH were added to the sample and the flask refluxed on a water bath for 15 min. When the solution had cooled 3-5 ml of 30% hydrogen peroxide was added and gently heated. This solution was acidified with acetic acid adding 6 ml of 10% solution in excess. To the electrolytic cell l/125 of this solution and 28 ml 10% KNoj were added withexclusion of light and Ag ions were generated. The current was of the order of 5 mA. The end-point was determined amperometrically with 3 different indicator electrode systems: (1) with 2 polarized Ag electrodes (200 mV); (2) with Ag and Pt electrodes, polarized with 200 mV; and (3) with a Cu-Ag electrode system without external voltage. In all the systems the results were consistent, the error being less than 1%. Ad. Hu. 14551 COULOMETRIC DETERMINATION OF CYCLOHEXAL AND HEXOBARBITONE. K.Kalinowski and H.Baran. (Dept. of Pharmaceutical Chemistry, Academy of Medicine, L,6dL, Poland.) Acta Pharm. Polon., 16 (1959) 231. acid) (CH) and hexobarbitone Cyclohexal 5 - A1 cyclohexenyl-5-ethylbarbituric acid) (HB) were determined by (N-methyl-5-A i -cyclohexenyl-5-methylbarbituric coulometric titration at constant current with electrolytically generated chlorine. For determination of CH 10 ml of about 0.02% solution of CH was mixed with 10 ml of 10% HCl. In HB determination 10 ml of 5% HCl was used. The end-point was determined by amperometry with 2 polarized Pt electrodes. The error of determination was less than 0.7%. Ad. Hu. See also: [255,272,394].
8. ELECTROPHORESIS I4561 A NEW METHOD FOR STAINING PAPER ELECTROPHEROGRAMS. wa. Biokhimiya, 24 (1959) 127.
M. N. Jegoro-
The paper is stained by means of 0.08% solution of acid-deep-blue in 10% AcOH to eliminate the excess dye; the stained bands are eluted with 0.1 N NaOH and the optical densities at 520 mu read with a calorimeter. The intensities are stable for at least 30 min. Ca. Cas. ]4571 EVALUATION OF ELECTROPHORESIS DIAGRAMS. Sorin M. Idu and Nicolae B. Cucu. Hoppe-Seyler’s Z. physiol. Chem. , 314 (1959) 284. With the aid of a simple algebraic method and of a suitably traced diagram method of Wallner Ulke for measuring areas under electrophoresis diagrams’ been simplified.
the has