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A newly discovered entomopoxvirus of Choristoneura diversana (Lepidoptera: Tortricidae)
JOURNAL
OF INVERTEBRATE
A Newly
PATHOLOGY
Discovered
22, 300-302 (1973)
Entomopoxvirus of Choristoneora (Lepidoptera: Tortricidae)
diversana
280’~ 220 Choristoneura diversana is a serious pest They measured approximately sections. Some virions of the Todomatsu fir, Abies skchalinensis, nm in longitudinal in Hokkaido, Japan. For the last few years had electron-dense cores, averaging 210 X a high population of this insect has been 110 nm, surrounded by two layers. observed. In June, 1972, a new disease ocThe virions observed in C. diversana curred simultaneously with a nuclear polythe entomopoxvirus closely resembled hedrosis among full-grown larvae that were (M. Bergoin, J. C. Veyrunes, and C. Vago., collected from the high population area and C. R. Acad. Sci. Ser. D, 269,1464-1466, were reared by Dr. Kamijo of Hokkaido 1969) first described in Melolontha meloForest Experiment Station, for the purpose lontha by C. Vago (J. Invertebr. Pathol. 5, of mass propagation of the hymenopterous 275-276, 1963) and later reported in Lepidoptera (J. Weiser and C. Vago. J. parasites. Invertebr. Pathol. 8, 314319, 1966; G. R. A light microscope examination revealed a number of ovoid inclusion bodies in the Sutter, J. Inuertebr. Pathol. 19, 375382, disintegrated adipose tissue. The ovoid in- 1972; D. W. Roberts and R. R. Granados. clusion bodies averaged 4.6 X 3.1 pm in J. Invertebr. Pathol. 12, 141-143, 1968; J. Fosset, C. Vago, J.-L. size, but often measured 7.0 X 5.0 pm or G. Meynadier, Duthiot, and N. Bres, Ann. Epiphyties 19, larger (Fig. 1). By electron microscope studies it was 703-706, 1968; F. T. Bird, C. J. Sanders, found that several poxlike virions were oc- and J. M. Burke. J. Invertebr. Pathol. 18, cluded within an inclusion body. The vi- 159-161, 1971). rions were oval and had a beaded surface. In sections some electronlucent zones,
FIG.
1. Inclusion
bodies in the disintegrated
300 Copyright All rights
@ 1973 by Academic Press, Inc. of reproduction in any form reserved.
content
of Chorktoneura
diversana.
30 I
KOTES
FIG.
which
2.
have
Sections of the angular
the inclusion bodies with several outline and the crystalline structures.
virions
and
electronlucent
mm+
302
NOTES
which have neither cores nor beaded surface, were observed (Fig. 2). They have crystalline lattice whose orientation is different from that observed in the inclusion body matrix. They have the clearly defined angular outline, while the occluded virions have the undefined circular or oval outline. Bird and coworkers have observed similar structures within the inclusion bodies of entomopoxvirus of Choristoneura biennis. But those structures would not be virions. Possibly they would be fusiform crystalline inclusions produced separately in the cell and would become occluded in the inclusion
bodies in the same time as the mature virus particles. To clarify the nature and the process of formation of them, further studies are necessary. The virus can be transmitted to a fir budworm, Archippus isshikii. K. KATAGIRI Asakawa Forest Government Forest Experiment Station Nagafusacho 1833, Hachioji, Tokyo, Japan Received December 22, 1972
OF INVERTEBRATE
A Newly
PATHOLOGY
Discovered
22, 300-302 (1973)
Entomopoxvirus of Choristoneora (Lepidoptera: Tortricidae)
diversana
280’~ 220 Choristoneura diversana is a serious pest They measured approximately sections. Some virions of the Todomatsu fir, Abies skchalinensis, nm in longitudinal in Hokkaido, Japan. For the last few years had electron-dense cores, averaging 210 X a high population of this insect has been 110 nm, surrounded by two layers. observed. In June, 1972, a new disease ocThe virions observed in C. diversana curred simultaneously with a nuclear polythe entomopoxvirus closely resembled hedrosis among full-grown larvae that were (M. Bergoin, J. C. Veyrunes, and C. Vago., collected from the high population area and C. R. Acad. Sci. Ser. D, 269,1464-1466, were reared by Dr. Kamijo of Hokkaido 1969) first described in Melolontha meloForest Experiment Station, for the purpose lontha by C. Vago (J. Invertebr. Pathol. 5, of mass propagation of the hymenopterous 275-276, 1963) and later reported in Lepidoptera (J. Weiser and C. Vago. J. parasites. Invertebr. Pathol. 8, 314319, 1966; G. R. A light microscope examination revealed a number of ovoid inclusion bodies in the Sutter, J. Inuertebr. Pathol. 19, 375382, disintegrated adipose tissue. The ovoid in- 1972; D. W. Roberts and R. R. Granados. clusion bodies averaged 4.6 X 3.1 pm in J. Invertebr. Pathol. 12, 141-143, 1968; J. Fosset, C. Vago, J.-L. size, but often measured 7.0 X 5.0 pm or G. Meynadier, Duthiot, and N. Bres, Ann. Epiphyties 19, larger (Fig. 1). By electron microscope studies it was 703-706, 1968; F. T. Bird, C. J. Sanders, found that several poxlike virions were oc- and J. M. Burke. J. Invertebr. Pathol. 18, cluded within an inclusion body. The vi- 159-161, 1971). rions were oval and had a beaded surface. In sections some electronlucent zones,
FIG.
1. Inclusion
bodies in the disintegrated
300 Copyright All rights
@ 1973 by Academic Press, Inc. of reproduction in any form reserved.
content
of Chorktoneura
diversana.
30 I
KOTES
FIG.
which
2.
have
Sections of the angular
the inclusion bodies with several outline and the crystalline structures.
virions
and
electronlucent
mm+
302
NOTES
which have neither cores nor beaded surface, were observed (Fig. 2). They have crystalline lattice whose orientation is different from that observed in the inclusion body matrix. They have the clearly defined angular outline, while the occluded virions have the undefined circular or oval outline. Bird and coworkers have observed similar structures within the inclusion bodies of entomopoxvirus of Choristoneura biennis. But those structures would not be virions. Possibly they would be fusiform crystalline inclusions produced separately in the cell and would become occluded in the inclusion
bodies in the same time as the mature virus particles. To clarify the nature and the process of formation of them, further studies are necessary. The virus can be transmitted to a fir budworm, Archippus isshikii. K. KATAGIRI Asakawa Forest Government Forest Experiment Station Nagafusacho 1833, Hachioji, Tokyo, Japan Received December 22, 1972