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A polyclonal antiserum raised against quail embryonic primordial germ cells
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A POLYCLONAL ANTISERUM RAISED AGAINST QUAIL EMBRYONIC PRIMORDIAL GERMCELLS.M. Ginsbur~, ~. Hochman and H. E y a l - G i l a d i . Department of Zoology, The I n s t i t u t e of L i f e Sciences, The Hebrew U n i v e r s i t y of Jerusalem, Jerusalem 91904, I s r a e l .
GE~IMAL GRANULES OF ~ PLASMARE SYNTHESISED ~.7~?DURINGOOCYTEMATURATIONIN X. LAEVIS. A. Gray' A. Jurandx'2 and K.E. Dixonx. XSchoolof Biological Sciences, FlindersUniversity of South Australia and 21nstitute of Animal Genetics, Edinburgh University.
A polyclonal antiserum was produced by immunizing rabbits against primordial germ c e l l s (PGCs), isolated from gonads of 7 days quall embryos. The antiserum which reacted s p e c i f l c a l I y wlth the cytoplasm of the gonadal PGCs of both sexes, expressed a very strong and i n v a r i a b l e reaction with a l l the c e l l s of both u t e r i n e ( l l l ; V l l E.G 6 K) and e a r l y l a i d (X to XIII E.G & K) stages. At stage X I I I a few big c e l l s in the hypoblast i c layer reacted stronger than t h e i r neighbours. Throughout g a s t r u l a t i o n , the react i o n gradually disappeared from most of the e p i b l a s t , mlddle layer and the d e f i n i t i v e entoderm of the lower layer. I t remained prominent in the primary hypoblast and in some scattered c e l l s e i t h e r ingressing from the e p i b l a s t or situated 1 a t e r a l l y and ant e r i o r l y to the PS. These results might indicate that PGCs share common antigens with t o t i p o t e n t young embryonic c e l l s . These antigens are probably lost from somatic c e l l s during f u r t h e r development while at least some of them remain preserved in the PGCs.
In late st 4 or 5 oscytesof X. lae~is grot~r~ of mit~ria Iie in the vegetal subcortex. In st 6 oscytes from frogs which had not beenexposedto gonadotrophichormonesnarrowelectron-densestrips appear between adjacent mitochondria.After st 6 oocytes weretreated in vitz~ with progesteroneor chorionic gonadotrophin (also in rive) or injected with ~ aggregatesof germinalgranulesappeared for the first time. By 4-5 h after treatn~t began, the intermitochondrial strips lengthened and thickened and from about 6 h they appearedas loosely structured aggregatesof germinal granules. By 9-10 h these moved from between the mitochondria, became more compact and closely resembled aggregates of germinal granules in unfertili~d eggs. Cycloheximide,erythromycinand chloramphenicol prevented appearanceof aggregates of germinal granules and the intermitochondrial strips remained unchanged. We conclude that aggregates of germinal grart~les, believed to be the determinant of germcells, are synthesisedduring oocyte maturation from precursor materialwhich appears late in osgenesis. This synthesis requires both cytoplas.mic and mitochondrial protein synthesis.
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Variations of transferrin in ~eminiferous tubules of rats with age and sterility. J .M.Gull Iaumin+M.T. Hocher eau-de Reviers,x x + C.Perreau,P.Bardos. *Immunology Dpt,Faculty of Medicine.37TOURS ~Physiology of Reproduction.37380.NOUZILLY FRANCE. Wistar rats were killed at23,46 and 150 days of age.Seminiferous tubul=s were separated,mixed and the supernatent(40000g) recuperated.Transferrin wasdetermined, b~ ~IA. The seminiferous tubule transferrin concentration decreased with age(23d:390~g/ml;. 46d:97+18;150d:59+14;in sterile rats:95+11 The ~otal testicular content increased slightly with development of spermatogenesis but not parallely to germ cell number increase :23d :50~g ;46d :73rag;150d :89pg/testis In sterile rats it was equal to 21/ag/t. However the total number of Sertoli cells per testis is reduced by half in such animals(Viguier-Martinez et ai1984). In conclusion,transferrin in rat seminiferous tubules increased relatively slightly with spermatogenesis establishment and is not drastically decreased by absence of germ cells in the testis.
ELECTRON MICROSCOPIC OBSERVATION ON ANNULATE LAMELLAE DURING OOGENESIS IN GOLDFISH(Car assius auratus). Huang Guoping and Yan Shaoyi Institute of Developmental Biology,Academia Sinica,Beijing,China. Morphological changes of annulate lamellae during oogenesis in goldfish have been studied. The development of goldfish oocytes are devided into 5 stages. In every stage, the distribution and arrangement of annulate lamellae have been observed under electron microscope. It was found that as oocytes are growing,the possibility of annulate lamellae being found and the number of annulate lamellae in a stalk are increasing gradually.Except in oogonium stage,annulate lamellae frequently appear in the ooplasm distal away from the nucleus.It is noted that pore-associated and interlamellar materials in many instances appear to be continuous with polyribosomes.According to the evidence which have been observed,it is proposed that annulate lamellae in goldfish oocytes are probably differentiated from the rough endoplasmic reticulum.
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141
A POLYCLONAL ANTISERUM RAISED AGAINST QUAIL EMBRYONIC PRIMORDIAL GERMCELLS.M. Ginsbur~, ~. Hochman and H. E y a l - G i l a d i . Department of Zoology, The I n s t i t u t e of L i f e Sciences, The Hebrew U n i v e r s i t y of Jerusalem, Jerusalem 91904, I s r a e l .
GE~IMAL GRANULES OF ~ PLASMARE SYNTHESISED ~.7~?DURINGOOCYTEMATURATIONIN X. LAEVIS. A. Gray' A. Jurandx'2 and K.E. Dixonx. XSchoolof Biological Sciences, FlindersUniversity of South Australia and 21nstitute of Animal Genetics, Edinburgh University.
A polyclonal antiserum was produced by immunizing rabbits against primordial germ c e l l s (PGCs), isolated from gonads of 7 days quall embryos. The antiserum which reacted s p e c i f l c a l I y wlth the cytoplasm of the gonadal PGCs of both sexes, expressed a very strong and i n v a r i a b l e reaction with a l l the c e l l s of both u t e r i n e ( l l l ; V l l E.G 6 K) and e a r l y l a i d (X to XIII E.G & K) stages. At stage X I I I a few big c e l l s in the hypoblast i c layer reacted stronger than t h e i r neighbours. Throughout g a s t r u l a t i o n , the react i o n gradually disappeared from most of the e p i b l a s t , mlddle layer and the d e f i n i t i v e entoderm of the lower layer. I t remained prominent in the primary hypoblast and in some scattered c e l l s e i t h e r ingressing from the e p i b l a s t or situated 1 a t e r a l l y and ant e r i o r l y to the PS. These results might indicate that PGCs share common antigens with t o t i p o t e n t young embryonic c e l l s . These antigens are probably lost from somatic c e l l s during f u r t h e r development while at least some of them remain preserved in the PGCs.
In late st 4 or 5 oscytesof X. lae~is grot~r~ of mit~ria Iie in the vegetal subcortex. In st 6 oscytes from frogs which had not beenexposedto gonadotrophichormonesnarrowelectron-densestrips appear between adjacent mitochondria.After st 6 oocytes weretreated in vitz~ with progesteroneor chorionic gonadotrophin (also in rive) or injected with ~ aggregatesof germinalgranulesappeared for the first time. By 4-5 h after treatn~t began, the intermitochondrial strips lengthened and thickened and from about 6 h they appearedas loosely structured aggregatesof germinal granules. By 9-10 h these moved from between the mitochondria, became more compact and closely resembled aggregates of germinal granules in unfertili~d eggs. Cycloheximide,erythromycinand chloramphenicol prevented appearanceof aggregates of germinal granules and the intermitochondrial strips remained unchanged. We conclude that aggregates of germinal grart~les, believed to be the determinant of germcells, are synthesisedduring oocyte maturation from precursor materialwhich appears late in osgenesis. This synthesis requires both cytoplas.mic and mitochondrial protein synthesis.
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143
Variations of transferrin in ~eminiferous tubules of rats with age and sterility. J .M.Gull Iaumin+M.T. Hocher eau-de Reviers,x x + C.Perreau,P.Bardos. *Immunology Dpt,Faculty of Medicine.37TOURS ~Physiology of Reproduction.37380.NOUZILLY FRANCE. Wistar rats were killed at23,46 and 150 days of age.Seminiferous tubul=s were separated,mixed and the supernatent(40000g) recuperated.Transferrin wasdetermined, b~ ~IA. The seminiferous tubule transferrin concentration decreased with age(23d:390~g/ml;. 46d:97+18;150d:59+14;in sterile rats:95+11 The ~otal testicular content increased slightly with development of spermatogenesis but not parallely to germ cell number increase :23d :50~g ;46d :73rag;150d :89pg/testis In sterile rats it was equal to 21/ag/t. However the total number of Sertoli cells per testis is reduced by half in such animals(Viguier-Martinez et ai1984). In conclusion,transferrin in rat seminiferous tubules increased relatively slightly with spermatogenesis establishment and is not drastically decreased by absence of germ cells in the testis.
ELECTRON MICROSCOPIC OBSERVATION ON ANNULATE LAMELLAE DURING OOGENESIS IN GOLDFISH(Car assius auratus). Huang Guoping and Yan Shaoyi Institute of Developmental Biology,Academia Sinica,Beijing,China. Morphological changes of annulate lamellae during oogenesis in goldfish have been studied. The development of goldfish oocytes are devided into 5 stages. In every stage, the distribution and arrangement of annulate lamellae have been observed under electron microscope. It was found that as oocytes are growing,the possibility of annulate lamellae being found and the number of annulate lamellae in a stalk are increasing gradually.Except in oogonium stage,annulate lamellae frequently appear in the ooplasm distal away from the nucleus.It is noted that pore-associated and interlamellar materials in many instances appear to be continuous with polyribosomes.According to the evidence which have been observed,it is proposed that annulate lamellae in goldfish oocytes are probably differentiated from the rough endoplasmic reticulum.
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