- Email: [email protected]
A practical guide to protein and peptide purification for microsequencing. Second edition
602
Book Reviews
Many proteins are very easily denatured and care has to be taken in their use. This book provides much of the essential information. It deals with: in vitro characterization; analytical chromatography; internal structure and organization; solution properties; posttranslational processing; fragmentation; peptide sequence determination; electrophoretic technique; production and application of polyclonal and monoclonal antibodies; conformational stability; hydration; recombinant protein technology; storage stabilization, and process purification. Proteins such as interferons, blood factors, hormones, etc. are becoming more available on a commercial scale and this volume will be useful to workers involved in processing such proteins. A Practical Guide to Protein and Peptide Purification for Microsequencing. Second Edition-Edited by P. MATSUDAIRA. 184~~. 1993. Academic Press, San Diego. The first edition was concerned with the question “How can I obtain the N-terminal sequence?’ This second edition is concerned with “How can I obtain useful sequence information?” The topics deal with; obtaining partial sequences from < 500 pmol of protein; enzymic digestion and HPLC peptide isolation; purification by SDS-PAGE; sequence analysis after in situ protease digestion on nitrocellulose; MS characterization; sequences of commonly used preoteases. There is also an updated reference list. RNA Editing; The Alteration of Protein Coding Sequences of RNA-Edited by R. BENNE. 196 pp. 1993. Ellis Horwood, Chichester, U.K. f39.95. The four uridines in mitochondrial transcript for the cytochrome oxidase subunit 2 in Trypanosomes are not encoded in the DNA. The RNAs are edited by massive transcriptional U-insertions and U-deletions. This book deals with RNA editing in mitochondria of: Trypanosomes, Leishmania, and Physarium polycephalum; RNA editing of paramyxovirus P gene mRNA; mammalian apoliporotein B mRNA; RNA in plant organelles; double stranded RNA adenosine deaminase-a potential agent for RNA editing? The editing signals may allow the production of more than one protein by one gene, allow greater compactness, allow the removal of frame shifts, the creation of stop/start codons, and multiple codon changes. The genetic code is very complex and subtle but that is to be expected after about a billion years of development! Asymmetric Synthesis of Natural Products-A. KOSKINEN. 234~~. 1993. Wiley, Chichester, U.K. Paperback f16.95. Most natural products are chiral and many are also asymmetric. This book deals with: chirality, topology and asymmetric compounds; asymmetric synthesis [carbonyl carbon, enolates, olefins]; carbohydrates; amino acids, peptides and proteins; nucleosides, nucleotides and nucleic acids; polyketides; isoprenoids; shikimic acid derivatives; alkaloids. It gives good insight into the nature of the problems in such syntheses and the structural chemistry of many important compounds. The reader will get a much better understanding of the structural relationship of many important drugs and natural products. It also has interesting asides such as “The aroma of sunburnt beer is structurally related to the odor substances of North American skunk and cat’s urine”.
Photosynthesis; Molecular, Physiological and Environmental Processes. Second Edition-D. W. LAWTON. 318 pp. 1993. Longman. Paperback f 19.99. The first edition of this book was published in 1987 and reprinted in 1990. This new edition deals with the major topics; light, the driving force in photosynthesis (P); energy capture in P; the P apparatus; electron and proton transport; synthesis of ATP; chemistry of P; metabolism of P products; C4-P and CAM; molecular biology of P; carbon dioxide supply; P by leaves; plant P production. The information has been brought up to date and the new material includes: the crystalization of the P reaction centre of purple bacteria and high resolution X-ray analysis of the structure resulting in increased understanding of the primary events in P; the X-ray analysis of ribulose biphosphate carboxylase-oxygenase; the genetic mapping of chloroplast DNA and the location of the genes for chloroplast constituents; global climate changes and the increased levels of carbon dioxide in the atmosphere. Protein Phosphorylation; a Practical Approach-Edited by D. G. HARDY. 300 pp. 1993. IRL/Oxford University Press. Hardback $59. Protein phosphorylation (PP) is the major mechanism by which cells respond to extracellular signals such as hormones and growth factors. It is also responsible for the timing of events which must occur at defined stages in the cell cycle such as DNA synthesis and mitosis. PP is catalysed by protein kinases and reversed by protein phosphorylases. This book deals with: PP in intact cells; phosphopeptide mapping and phosphoamino acid analysis on cellulose thin layer plates; analysis of sites phosphorylated in uipo and in u&-o; use of serine/thronine kinase activators and inhibitors to study PP in intact cells; use of PP inhibitors in intact cells; purification of mammalian serine/threonone kinases; purification of tyrosine kinases; identification of cDNAs that encode protein kinases; assay and purification of serine/threonine phosphatases; characterization of tyrosine phosphatases; identification of serine/threonine phosphatase genes in yeast, Drosophila and Man; protein kinase/phosphatase specificity using synthetic peptides. A better understanding of activators and inhibitors of PP systems will provide the basis for new drugs that can be used in cancer therapy. Aspects of Synaptic Transmission; ACh, Sigma Receptors, CCK, Eicosanoids and Neurotoxins-Edited by T. W. STONE. 255 pp. 1993. Taylor & Francis. f45. This is the published proceedings of the second Kelvin Conference. The subjects reviewed are: neuronal muscarinic receptor subtypes; cholinergic pathways in the CNS; cholinergic neurones and memory: nicotinic cholinergic stimulation in animal models of behaviour; sigma receptors [receptors for benzomorphans]; CCK-A and CCK-B in analgesia; CCK receptors; neuronal release of arachidonic acid; presynaptic toxins [TTX, conotoxins, scorpion toxins. anemone toxins, dendrotoxins, bee venom, phospholipase A2, apamin, scyllatoxin]; alpha latrotoxin; spider toxin and glutamate receptors; maitotoxin and calcium homeostasis. Opioids I and II-Edited by A. HERZ. Handbook of Experimental Pharmacology Vol. 104, parts I and II. Opioids I. 815 pp. 1993. Springer, Berlin. DM580. Opioids II 840 pp. 1993. Springer, Berlin. DM580.
Book Reviews
Many proteins are very easily denatured and care has to be taken in their use. This book provides much of the essential information. It deals with: in vitro characterization; analytical chromatography; internal structure and organization; solution properties; posttranslational processing; fragmentation; peptide sequence determination; electrophoretic technique; production and application of polyclonal and monoclonal antibodies; conformational stability; hydration; recombinant protein technology; storage stabilization, and process purification. Proteins such as interferons, blood factors, hormones, etc. are becoming more available on a commercial scale and this volume will be useful to workers involved in processing such proteins. A Practical Guide to Protein and Peptide Purification for Microsequencing. Second Edition-Edited by P. MATSUDAIRA. 184~~. 1993. Academic Press, San Diego. The first edition was concerned with the question “How can I obtain the N-terminal sequence?’ This second edition is concerned with “How can I obtain useful sequence information?” The topics deal with; obtaining partial sequences from < 500 pmol of protein; enzymic digestion and HPLC peptide isolation; purification by SDS-PAGE; sequence analysis after in situ protease digestion on nitrocellulose; MS characterization; sequences of commonly used preoteases. There is also an updated reference list. RNA Editing; The Alteration of Protein Coding Sequences of RNA-Edited by R. BENNE. 196 pp. 1993. Ellis Horwood, Chichester, U.K. f39.95. The four uridines in mitochondrial transcript for the cytochrome oxidase subunit 2 in Trypanosomes are not encoded in the DNA. The RNAs are edited by massive transcriptional U-insertions and U-deletions. This book deals with RNA editing in mitochondria of: Trypanosomes, Leishmania, and Physarium polycephalum; RNA editing of paramyxovirus P gene mRNA; mammalian apoliporotein B mRNA; RNA in plant organelles; double stranded RNA adenosine deaminase-a potential agent for RNA editing? The editing signals may allow the production of more than one protein by one gene, allow greater compactness, allow the removal of frame shifts, the creation of stop/start codons, and multiple codon changes. The genetic code is very complex and subtle but that is to be expected after about a billion years of development! Asymmetric Synthesis of Natural Products-A. KOSKINEN. 234~~. 1993. Wiley, Chichester, U.K. Paperback f16.95. Most natural products are chiral and many are also asymmetric. This book deals with: chirality, topology and asymmetric compounds; asymmetric synthesis [carbonyl carbon, enolates, olefins]; carbohydrates; amino acids, peptides and proteins; nucleosides, nucleotides and nucleic acids; polyketides; isoprenoids; shikimic acid derivatives; alkaloids. It gives good insight into the nature of the problems in such syntheses and the structural chemistry of many important compounds. The reader will get a much better understanding of the structural relationship of many important drugs and natural products. It also has interesting asides such as “The aroma of sunburnt beer is structurally related to the odor substances of North American skunk and cat’s urine”.
Photosynthesis; Molecular, Physiological and Environmental Processes. Second Edition-D. W. LAWTON. 318 pp. 1993. Longman. Paperback f 19.99. The first edition of this book was published in 1987 and reprinted in 1990. This new edition deals with the major topics; light, the driving force in photosynthesis (P); energy capture in P; the P apparatus; electron and proton transport; synthesis of ATP; chemistry of P; metabolism of P products; C4-P and CAM; molecular biology of P; carbon dioxide supply; P by leaves; plant P production. The information has been brought up to date and the new material includes: the crystalization of the P reaction centre of purple bacteria and high resolution X-ray analysis of the structure resulting in increased understanding of the primary events in P; the X-ray analysis of ribulose biphosphate carboxylase-oxygenase; the genetic mapping of chloroplast DNA and the location of the genes for chloroplast constituents; global climate changes and the increased levels of carbon dioxide in the atmosphere. Protein Phosphorylation; a Practical Approach-Edited by D. G. HARDY. 300 pp. 1993. IRL/Oxford University Press. Hardback $59. Protein phosphorylation (PP) is the major mechanism by which cells respond to extracellular signals such as hormones and growth factors. It is also responsible for the timing of events which must occur at defined stages in the cell cycle such as DNA synthesis and mitosis. PP is catalysed by protein kinases and reversed by protein phosphorylases. This book deals with: PP in intact cells; phosphopeptide mapping and phosphoamino acid analysis on cellulose thin layer plates; analysis of sites phosphorylated in uipo and in u&-o; use of serine/thronine kinase activators and inhibitors to study PP in intact cells; use of PP inhibitors in intact cells; purification of mammalian serine/threonone kinases; purification of tyrosine kinases; identification of cDNAs that encode protein kinases; assay and purification of serine/threonine phosphatases; characterization of tyrosine phosphatases; identification of serine/threonine phosphatase genes in yeast, Drosophila and Man; protein kinase/phosphatase specificity using synthetic peptides. A better understanding of activators and inhibitors of PP systems will provide the basis for new drugs that can be used in cancer therapy. Aspects of Synaptic Transmission; ACh, Sigma Receptors, CCK, Eicosanoids and Neurotoxins-Edited by T. W. STONE. 255 pp. 1993. Taylor & Francis. f45. This is the published proceedings of the second Kelvin Conference. The subjects reviewed are: neuronal muscarinic receptor subtypes; cholinergic pathways in the CNS; cholinergic neurones and memory: nicotinic cholinergic stimulation in animal models of behaviour; sigma receptors [receptors for benzomorphans]; CCK-A and CCK-B in analgesia; CCK receptors; neuronal release of arachidonic acid; presynaptic toxins [TTX, conotoxins, scorpion toxins. anemone toxins, dendrotoxins, bee venom, phospholipase A2, apamin, scyllatoxin]; alpha latrotoxin; spider toxin and glutamate receptors; maitotoxin and calcium homeostasis. Opioids I and II-Edited by A. HERZ. Handbook of Experimental Pharmacology Vol. 104, parts I and II. Opioids I. 815 pp. 1993. Springer, Berlin. DM580. Opioids II 840 pp. 1993. Springer, Berlin. DM580.