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A Schistosoma mansoni cercarial protease as a potential vaccine candidate for Schistosomiasis
248 O-0478
Oral Sessions I Parasitology
Internariond
USE OF PROTEIN A TO STUDY THE IN VITRO EFFECT OF IgG REMOVAL ON THE IgE AM) ITS RELEVANCE TO THE 1N VIVO ANTIBODY BLOCKING EFFECT IN CANAL SUDANESE CLEANERS WITH INFECTED SCHISTOSOMA MANSONI
Satti M.Z., Lind P.. Sulaiman S., Vennervald B. and Salih A.A.M. Tropic1 Medecine Research Institute, Khartoum The present work comprises a longitudinal study Schistosoma mansoni infection in 072 OccuDationally hyper-exposed canal cleaners in the Sudan. ELISA-Las used to detect total serum IgE, IgG, and IgG, and specific IgE and IgG, in response to whole worm antigen (WWH) and soluble egg antigen (SEA). Total IgG, was also highly elevated in the hyper-exposed canal cleaners with a sionificant decrease after treatment. Total serum IgG and albumin were determined by rocket immunoelectro-phoresis before and after protein A Total serum IgE and fractionation -of sera. schistosome soecific IqE and IqG, antibodies to whole worm tiomoqenate- (WWH) &id soluble e33 antigen (SEA) we& determined before and after orotein A fractionation of sera. usins the ELISA immunoglob;lins were technique. Total serum the canal hiqhly elevated, especially in canal The serum -samples from all cl&aners. cleaners showed a considerable deviation from the normal total IgG level, being up to eight times level. A negazive higher than normal the correlation was observed between serum albumin and total serum IgG, in chronically infected canal Specific IgE to Wh'H and SEA showed a cleaners. significant increase in canal cleaners after 'protein A depletion of IgG. In conclusion, the study shows an in vitro effect of IyG on IgE measurement, suggesting a blocking effect in vitro of IqG antibodies. c-----
47 (Suppl.) (1998) 133-281
O-0480 A SCflf.9TOSOM4 MXNSONfCERCARlAL AS A POTENTlAL VACCINE CANDIDATE SCHlSTOSOMlASlS
PROTEASE FOR
YOU&I Darani H.’ and Doenhoff M.J. * Department of Parasitology and Mycology, Faculty of Medicine, ShahrekordJran. * School of Biilogical Sciences, University of Wales Bangor, Gwynedd LL57 2UW. l
l
l
The immunological properties of a 27RSkDa serine protease of Schlstvsoma manson/ have been investigate. When inbred mice of two different strains were immunized wkh extracts containing the protease as a major constituent, only a minority of individuals in each group of animals produced antibody with specitIclty for the protease, This result indicated that immune responses against he 27RSkDa molecule were in some way modulated. The antibody‘responder’ mice we& however, protected against schistosoma mansoni infection to a greater degree than antibody ‘nonresponded and unimmunbed controls. Experiments in which other serine proteases were used for immunization indicated that the poor immunogenicity of the S. manson larval protease may be a general property of these enzymes and possibly a consequence of their ability to hydrolyze immunoglobulin or otherwise interfer with antigen processing and presentation. The opportunity that the results with the cercarial elastase provide for the development of a vaccine for schistosomiasis vitl be discussed.
Oral Sessions I Parasitology
Internariond
USE OF PROTEIN A TO STUDY THE IN VITRO EFFECT OF IgG REMOVAL ON THE IgE AM) ITS RELEVANCE TO THE 1N VIVO ANTIBODY BLOCKING EFFECT IN CANAL SUDANESE CLEANERS WITH INFECTED SCHISTOSOMA MANSONI
Satti M.Z., Lind P.. Sulaiman S., Vennervald B. and Salih A.A.M. Tropic1 Medecine Research Institute, Khartoum The present work comprises a longitudinal study Schistosoma mansoni infection in 072 OccuDationally hyper-exposed canal cleaners in the Sudan. ELISA-Las used to detect total serum IgE, IgG, and IgG, and specific IgE and IgG, in response to whole worm antigen (WWH) and soluble egg antigen (SEA). Total IgG, was also highly elevated in the hyper-exposed canal cleaners with a sionificant decrease after treatment. Total serum IgG and albumin were determined by rocket immunoelectro-phoresis before and after protein A Total serum IgE and fractionation -of sera. schistosome soecific IqE and IqG, antibodies to whole worm tiomoqenate- (WWH) &id soluble e33 antigen (SEA) we& determined before and after orotein A fractionation of sera. usins the ELISA immunoglob;lins were technique. Total serum the canal hiqhly elevated, especially in canal The serum -samples from all cl&aners. cleaners showed a considerable deviation from the normal total IgG level, being up to eight times level. A negazive higher than normal the correlation was observed between serum albumin and total serum IgG, in chronically infected canal Specific IgE to Wh'H and SEA showed a cleaners. significant increase in canal cleaners after 'protein A depletion of IgG. In conclusion, the study shows an in vitro effect of IyG on IgE measurement, suggesting a blocking effect in vitro of IqG antibodies. c-----
47 (Suppl.) (1998) 133-281
O-0480 A SCflf.9TOSOM4 MXNSONfCERCARlAL AS A POTENTlAL VACCINE CANDIDATE SCHlSTOSOMlASlS
PROTEASE FOR
YOU&I Darani H.’ and Doenhoff M.J. * Department of Parasitology and Mycology, Faculty of Medicine, ShahrekordJran. * School of Biilogical Sciences, University of Wales Bangor, Gwynedd LL57 2UW. l
l
l
The immunological properties of a 27RSkDa serine protease of Schlstvsoma manson/ have been investigate. When inbred mice of two different strains were immunized wkh extracts containing the protease as a major constituent, only a minority of individuals in each group of animals produced antibody with specitIclty for the protease, This result indicated that immune responses against he 27RSkDa molecule were in some way modulated. The antibody‘responder’ mice we& however, protected against schistosoma mansoni infection to a greater degree than antibody ‘nonresponded and unimmunbed controls. Experiments in which other serine proteases were used for immunization indicated that the poor immunogenicity of the S. manson larval protease may be a general property of these enzymes and possibly a consequence of their ability to hydrolyze immunoglobulin or otherwise interfer with antigen processing and presentation. The opportunity that the results with the cercarial elastase provide for the development of a vaccine for schistosomiasis vitl be discussed.