A short DNA sequence from λ phage inhibits protein synthesis in Escherichia coli rap

d. Mol. Hid. (19!W) 219, 377

Erratum A Short DNA

Sequence from il Phage Inhibits Protein Synthesis in Escherichia coli rap

David Perez-Morga

and Gabriel

Guarneros

J. #ol. Riol. (1990) 216. x--“50

Figure

C(a) and (In) wa,s incorrect’ly

printed.

0

The correct

2

figure

and legend is given 6

4

(min)

bar+

bar

(a) 0

6

4

2

(min)

bar+

bar

Strain

Half-life mRNA (min) bar b/a 35 4.1

Plasmid bar+ bar bar+

cl.0

3.’

1.2

3.8

rap+

(cl Figure 6. hln and bar tnRNAs stability. (‘ell cultures in log growth phase were induced for 5 min at 42°C and treat)ed with rifampicin (02 mg/ml). Portions were withdrawn at’ the indicated times after rifampicin addition. The RZU‘A was extracted, dot-blott)ed onto nibrocellulose. and hybridized to 32P-labeled RNA probes corresponding t,o bnr or bla. The filters were washed and autoradiographed. (a) hla RNA and (b) bar RNA in E. colt’ rap with pPCll0 (bnr’) or pPGlll (Dar-) are shown. (r) The half-lives derivrd from this experiment are summarized.

377

below