- Email: [email protected]
A simplified technique for measurement of blood volume with RISA
REPORT
OF M E E T I N G
T h e S o c i e t y of N u c l e a r M e d i c i n e - - T h i r d
91-23
Annual Meeting
June 1956, Salt Lake City, Utah President MILO HARRIS Chairmen of sessions
MAXWELL M. WINTROBE GEORGE E. CARTWRIGHT LT. CDR. NATHANIEL I. BERLIN United States Navy, Medical Corps, Armed Forces Special Weapons Project, Washington, D.C. REX L. HUFF
HYMER L. FRIEDELL R. R. NEWELL TOM CARLILE HANS HECHT NORMANJ. HOLTER HENRY L. JAFFE JoE LEWIS
Abstracts of papers presented A S i m p l i f i e d T e c h n i q u e for M e a s u r e m e n t o f B l o o d V o l u m e W i t h R I S A : PAUL R.
NOBLE, JOHN G. Fox, and C. R. PERRYMAN, Pittsburgh, Pa. The technique of blood- and plasmavolume measurement by radioactive isotopes has been well established using RISA, Cr 51 and Fe 59. In an effort to refine the R I S A technique for routine clinical application the following method has been evolved. The preparation of a standard is time consuming and a source of technical error. This step has been eliminated by using a counting technique to arrive at a ratio of counts per second for a given source in different geometries relative to a well counter. This ratio allows us to compare by simple arithmetic the injected source and a four milliliter sample of blood or plasma withdrawn after the usual mixing time. Errors due to incomplete mixing of a standard, volatilization of iodine or deposition on the volumetric flask wall are avoided. Time-consuming preparation of a standard is also avoided. In our laboratory the blood volume, plasma volume, and
hematocrit are available to the surgeon within thirty minutes when required, and the procedure may be repeated as often and as soon as necessary. O u r measurements indicate a potential technical error of 3 - 5 % , which we believe is satisfactory for routine clinical application. We do not recommend the technique as a research tool in situations where a higher degree of accuracy is required. S t u d i e s o n B l o o d V o l u m e in M a n I. T o t a l Blood, P l a s m a , and R e d - C e l l V o l u m e in N o r m a l S u b j e c t s : ROBERT E. ZIPF, JOE
M. WEBBER, and G. RICHARD GROVE. The facile application of radioisotope techniques to the precise determination of blood volume has provided an indispensible laboratory procedure for the clinical control of body-fluid and blood-volume alterations in many medical and surgical patients. The significance of these determinations is wholly dependent upon the ability of the clinician to establish accurate theoretical normal blood-, plasma-, and red-cell-volume 123
OF M E E T I N G
T h e S o c i e t y of N u c l e a r M e d i c i n e - - T h i r d
91-23
Annual Meeting
June 1956, Salt Lake City, Utah President MILO HARRIS Chairmen of sessions
MAXWELL M. WINTROBE GEORGE E. CARTWRIGHT LT. CDR. NATHANIEL I. BERLIN United States Navy, Medical Corps, Armed Forces Special Weapons Project, Washington, D.C. REX L. HUFF
HYMER L. FRIEDELL R. R. NEWELL TOM CARLILE HANS HECHT NORMANJ. HOLTER HENRY L. JAFFE JoE LEWIS
Abstracts of papers presented A S i m p l i f i e d T e c h n i q u e for M e a s u r e m e n t o f B l o o d V o l u m e W i t h R I S A : PAUL R.
NOBLE, JOHN G. Fox, and C. R. PERRYMAN, Pittsburgh, Pa. The technique of blood- and plasmavolume measurement by radioactive isotopes has been well established using RISA, Cr 51 and Fe 59. In an effort to refine the R I S A technique for routine clinical application the following method has been evolved. The preparation of a standard is time consuming and a source of technical error. This step has been eliminated by using a counting technique to arrive at a ratio of counts per second for a given source in different geometries relative to a well counter. This ratio allows us to compare by simple arithmetic the injected source and a four milliliter sample of blood or plasma withdrawn after the usual mixing time. Errors due to incomplete mixing of a standard, volatilization of iodine or deposition on the volumetric flask wall are avoided. Time-consuming preparation of a standard is also avoided. In our laboratory the blood volume, plasma volume, and
hematocrit are available to the surgeon within thirty minutes when required, and the procedure may be repeated as often and as soon as necessary. O u r measurements indicate a potential technical error of 3 - 5 % , which we believe is satisfactory for routine clinical application. We do not recommend the technique as a research tool in situations where a higher degree of accuracy is required. S t u d i e s o n B l o o d V o l u m e in M a n I. T o t a l Blood, P l a s m a , and R e d - C e l l V o l u m e in N o r m a l S u b j e c t s : ROBERT E. ZIPF, JOE
M. WEBBER, and G. RICHARD GROVE. The facile application of radioisotope techniques to the precise determination of blood volume has provided an indispensible laboratory procedure for the clinical control of body-fluid and blood-volume alterations in many medical and surgical patients. The significance of these determinations is wholly dependent upon the ability of the clinician to establish accurate theoretical normal blood-, plasma-, and red-cell-volume 123