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A001 Differential changes in vascular e-NOS and i-NOS during doca-salt hypertension
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D I F F I E V R e E AA N T A IS A- L I EL C NCT D H O EA U TN AN HNR G E L EL A SI i D D - HUO Y C P R - DIRT OE ESEDE NN S E USA S IL IR N E A N V P LA T K P H a A a A Ca A wd , . D e p ra H S O a Y n p bT K Cr P gD P . E Ht Al e b a R i - TOo .i a, M i R c& D Pr oU h L c yi o i*u Cen i A l d.M o sa i lt R a r Aes u cA p vcep& o rSo i r oi n ap gy c l L K o4 & D uE m 0i e C rs gD P v2e nU h iL c &K 4oesl Cu &p r uie2ni i t0io, s r Lyayl9o M C U e C n J d ih i h v a D Ec e im i Cr i p Me C s U ee e t h a n a n a , y r n J be g ib a c c o sn r ni o tn s t e(u t i l ch A g o t e iTr eoi aei m l n n c danf du oot if eoe ra ah El x d n t la x t l so v N o i l Y dsph v a t s i e o o ( a a v i d e re ai i r Om t tb e b pr i p cc e a c oN r na a(l l e tsu i r a nob n s t . r h yO s ap I t er N rr s t te d nc dr . a n h ee a k hn du u rs l O es i uae to e d p h ( u e Pc n E oi yr ) nh l -t t e h i i rp h iu t n c gi a nd ha e ndr c h y A r p f n e o o( r a irt m er r n n o a - n s t t v i er = o o nn gni n s ? e D H O (y r w Cpp o 1 ea A n r A en eS =( s n tl i r 6, v t r- , t fa n n2 a a u D I p c wr 5o d o P ( neOt Hlo i 3r (0 . t t d M e em n o r awg e s v ac r w A ( . e w w P + LlO ia iCs( N t Mc S h w e tA ) ed a Ia - p# v x s ( t b s o 1N cy a l ow n 5 O n3 tP r O g toe O hw 6 d ri aV (a c s) c iM tt a e d o P a r w A ot ei t i tC l 0 n- si H sE nh ar i E ; ew x 6 dh t t wa i e is h ( 1 w o e p w oa T r e c- h s n u n r h t i ( n So s 0N i- a r i ae a eln 3 .vO w t w L ( r - a w wae 3 Pe i r e a 0 a gd i ir s0 Ss t nm t n V Y eh i tne MS v P d oc w sa w eL o w i r w - i cer t a s woa P Nap en r v h n wo ts o tg tn eA o e s rih E er t r o a et h ( n2 pwh b p v aooe ern e n t ( o Ae a gta hwe w o t u i tr e t ho h C S i e n i e a i w a I -p 1 L- c e ( e N t1 - w n oN e p r w i a N A O Sf e t we r aOv i -c h nA r i P n c E mo ci n n (o r i t rc ern m e co ri e i r n s eo a ds e woo Pa daAo n ac 9 S ut s dt t 7 a 5 * t i8 h (y . 5 a p 6r eh n w8 e % sM r r a4 l to S ea aet h E a xnpn e ng a 7 2e vd 3 % % v a 1 2 a * 6 e Sp r (s Ebe e ps a i l w n a. g ee A Mo - h 1 2 25w o -c a rl r eI p 2s L ip eP e ncE ct r ( N ot-y M r e i p r t i Av Da w i i een2 A fc 6l= n da cul s. i c om i h n y( t 6 4 p ra o e aN t 6 H r c( 4S t ti p 7 i s. 1e ai w r t ns0. o a e n s n E 4 +1 t i0 n o( . 2 3 ar 7 m e. sM 6h ( 0 S % o i p 2 2 ti tS h vyeEa 3n I Enp e 7 e. 0r %i d4 r 2 * % v a a f p e TS sc 5e r o Eh (o eeNp t c a u tsl e S w lad. n ao f e 7 M c l rt %d t s t P cu E so N- a gn t i h i t m ati dp e 1 ac af uu i 8 ,Nt t 7es cl i v E eo .a t 0 S t c 7i gH rn ( n oa h r vy Hm op P e o e o H (t r sS i Evs p ve l i e0 rh e e b iew p t n 2 i tns t e h e . a n evy e i e a ni r ac i - ad e i t ud d- si N c vu hu h nOi N dy O i e a c gCp v eh Tc t tD et e dA eO e i D n h O y( h CpC F ea u nt AtN- e w e r n c Sa an VA rr i ge M csda e o i t Stndl e do C T R o o e b r o Na b e s e c u n ( c C d l C F .e c o e ut n A K W N o D H ~ fD j ~ ~y N$ O Hf P
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spontaneous} h}Tcrtensive rats
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recorded from tbetail by physiograph ever? fortnightly for the duration of the study period of three months At the end of the trial. animals were sacrificed and NOS activity in blocd vessels was measured by
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p v r A c T c p w v18 Students’ t-test a P w u f ? a R o s s t f a t y t g b p w s ( c t h c a m r b w s t d 1 T d ( S H y H A N a b S w s l t W r ( . p S 0 . p O A a y d 7 a i t N b v o t o a s s s [email protected]. 7 P P n c w s r ( r SHR compared to W P a a a t groups s i t r n l ( p
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and Y2 (1=-O.721,P=0.044). [n all the treated groups NOS acti}it! and nitrite level shows a positive relatzon but it was not significant. In conclusion ]t was found that compared to WKY rats SHR has lower NOS acti~ily in blood vessels which upon treatment with antioxidant y-tocotrienol increased the NOS activit:, and concomitantly reduced the blood pressure T i t f
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UO RT T R E E AA IR TN N N TI I E N R D A OI E T O B H E R E e S H M eu K r i ng r
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II E P K M
of Biochemistry, Uniwrsiti Kehangsaan Malaysia. Kuala Lumpur Malajsia.
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Cardiology,
Kno//AG,
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W p h rs a s e b y vn h f n a i p r e r o te A i e tf d n r s ua h f e a t E r a Le 1 n T c tc 3 a ln e h - T u m n ne d odc w e- o r h m ra o lr t w t (r r a 2 ( O e n to i m dm a a o g c I b E r a a Le 2 T nl ( O tc a 2A am e s E r e a eLl 1n e ct c a t n h3 a a o i v o al b r o ( O mc gm / i 3 o S o d p o t i rI o La 2 o o h rn n d a2 o n e o m a p f ( r L r1 fte M e 3o T u s r M lf 2 h e p o i ( d g n o- r i L< 2 im c 2o m p L 0 wk . , eH y t ( r m sa d n b l g1 pe d io c r / ll ( m p A m p c 9 m e r w O . o fo . 0 l o a a b o tp c s p fL o li em U i b a I M d o b 1 e s m h 5 c a n r . r 2m r r w u T nc o ocL a a mh U b a a t l r t t m ap nd r i M d oe o o n l c o i ( m 3 A c W i o0 m i h nr n.o c H 9 c o eK r d n p t f o e b pr r i e l e a i R B o e A l o E l t o io s tn e i c yt mt m b p a h ro H i l c m e o n eo so w o a A i o a A nr a h we a n E n oi tc C b l f b p f a c r a w e en ct e ri io g l ro b A e mi ln m n co p o h e C t A b m e d t if b nf l o r c e a e bo E rl w r e e et c H a d o t d i o N OB t hn e p o b pr of h r d l e a o i t c i to l m et b i o s f a K A
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D I F F I E V R e E AA N T A IS A- L I EL C NCT D H O EA U TN AN HNR G E L EL A SI i D D - HUO Y C P R - DIRT OE ESEDE NN S E USA S IL IR N E A N V P LA T K P H a A a A Ca A wd , . D e p ra H S O a Y n p bT K Cr P gD P . E Ht Al e b a R i - TOo .i a, M i R c& D Pr oU h L c yi o i*u Cen i A l d.M o sa i lt R a r Aes u cA p vcep& o rSo i r oi n ap gy c l L K o4 & D uE m 0i e C rs gD P v2e nU h iL c &K 4oesl Cu &p r uie2ni i t0io, s r Lyayl9o M C U e C n J d ih i h v a D Ec e im i Cr i p Me C s U ee e t h a n a n a , y r n J be g ib a c c o sn r ni o tn s t e(u t i l ch A g o t e iTr eoi aei m l n n c danf du oot if eoe ra ah El x d n t la x t l so v N o i l Y dsph v a t s i e o o ( a a v i d e re ai i r Om t tb e b pr i p cc e a c oN r na a(l l e tsu i r a nob n s t . r h yO s ap I t er N rr s t te d nc dr . a n h ee a k hn du u rs l O es i uae to e d p h ( u e Pc n E oi yr ) nh l -t t e h i i rp h iu t n c gi a nd ha e ndr c h y A r p f n e o o( r a irt m er r n n o a - n s t t v i er = o o nn gni n s ? e D H O (y r w Cpp o 1 ea A n r A en eS =( s n tl i r 6, v t r- , t fa n n2 a a u D I p c wr 5o d o P ( neOt Hlo i 3r (0 . t t d M e em n o r awg e s v ac r w A ( . e w w P + LlO ia iCs( N t Mc S h w e tA ) ed a Ia - p# v x s ( t b s o 1N cy a l ow n 5 O n3 tP r O g toe O hw 6 d ri aV (a c s) c iM tt a e d o P a r w A ot ei t i tC l 0 n- si H sE nh ar i E ; ew x 6 dh t t wa i e is h ( 1 w o e p w oa T r e c- h s n u n r h t i ( n So s 0N i- a r i ae a eln 3 .vO w t w L ( r - a w wae 3 Pe i r e a 0 a gd i ir s0 Ss t nm t n V Y eh i tne MS v P d oc w sa w eL o w i r w - i cer t a s woa P Nap en r v h n wo ts o tg tn eA o e s rih E er t r o a et h ( n2 pwh b p v aooe ern e n t ( o Ae a gta hwe w o t u i tr e t ho h C S i e n i e a i w a I -p 1 L- c e ( e N t1 - w n oN e p r w i a N A O Sf e t we r aOv i -c h nA r i P n c E mo ci n n (o r i t rc ern m e co ri e i r n s eo a ds e woo Pa daAo n ac 9 S ut s dt t 7 a 5 * t i8 h (y . 5 a p 6r eh n w8 e % sM r r a4 l to S ea aet h E a xnpn e ng a 7 2e vd 3 % % v a 1 2 a * 6 e Sp r (s Ebe e ps a i l w n a. g ee A Mo - h 1 2 25w o -c a rl r eI p 2s L ip eP e ncE ct r ( N ot-y M r e i p r t i Av Da w i i een2 A fc 6l= n da cul s. i c om i h n y( t 6 4 p ra o e aN t 6 H r c( 4S t ti p 7 i s. 1e ai w r t ns0. o a e n s n E 4 +1 t i0 n o( . 2 3 ar 7 m e. sM 6h ( 0 S % o i p 2 2 ti tS h vyeEa 3n I Enp e 7 e. 0r %i d4 r 2 * % v a a f p e TS sc 5e r o Eh (o eeNp t c a u tsl e S w lad. n ao f e 7 M c l rt %d t s t P cu E so N- a gn t i h i t m ati dp e 1 ac af uu i 8 ,Nt t 7es cl i v E eo .a t 0 S t c 7i gH rn ( n oa h r vy Hm op P e o e o H (t r sS i Evs p ve l i e0 rh e e b iew p t n 2 i tns t e h e . a n evy e i e a ni r ac i - ad e i t ud d- si N c vu hu h nOi N dy O i e a c gCp v eh Tc t tD et e dA eO e i D n h O y( h CpC F ea u nt AtN- e w e r n c Sa an VA rr i ge M csda e o i t Stndl e do C T R o o e b r o Na b e s e c u n ( c C d l C F .e c o e ut n A K W N o D H ~ fD j ~ ~y N$ O Hf P
L
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M F
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spontaneous} h}Tcrtensive rats
‘
c
( t d ( w u
y K
(
r
a n
S w 1
of
w 1 c
recorded from tbetail by physiograph ever? fortnightly for the duration of the study period of three months At the end of the trial. animals were sacrificed and NOS activity in blocd vessels was measured by
[ s d S t r p 2 v 1 t a ( m S s p c y c
r
a
p v r A c T c p w v18 Students’ t-test a P w u f ? a R o s s t f a t y t g b p w s ( c t h c a m r b w s t d 1 T d ( S H y H A N a b S w s l t W r ( . p S 0 . p O A a y d 7 a i t N b v o t o a s s s [email protected]. 7 P P n c w s r ( r SHR compared to W P a a a t groups s i t r n l ( p
w 5
n
c
u
G
and Y2 (1=-O.721,P=0.044). [n all the treated groups NOS acti}it! and nitrite level shows a positive relatzon but it was not significant. In conclusion ]t was found that compared to WKY rats SHR has lower NOS acti~ily in blood vessels which upon treatment with antioxidant y-tocotrienol increased the NOS activit:, and concomitantly reduced the blood pressure T i t f
r K
m W p
i F A
t p m N
o o
s
N
o
T
B
H
UO RT T R E E AA IR TN N N TI I E N R D A OI E T O B H E R E e S H M eu K r i ng r
N
F’rec/inica/
w c in blocd w.sels
y
II E P K M
of Biochemistry, Uniwrsiti Kehangsaan Malaysia. Kuala Lumpur Malajsia.
d Y t (
N
A
y A H
S
Cardiology,
Kno//AG,
Ludvigsfraferr,
S OF L YS ne
LE3
AA I c
P N L t
A
n
Gerrrrarry
W p h rs a s e b y vn h f n a i p r e r o te A i e tf d n r s ua h f e a t E r a Le 1 n T c tc 3 a ln e h - T u m n ne d odc w e- o r h m ra o lr t w t (r r a 2 ( O e n to i m dm a a o g c I b E r a a Le 2 T nl ( O tc a 2A am e s E r e a eLl 1n e ct c a t n h3 a a o i v o al b r o ( O mc gm / i 3 o S o d p o t i rI o La 2 o o h rn n d a2 o n e o m a p f ( r L r1 fte M e 3o T u s r M lf 2 h e p o i ( d g n o- r i L< 2 im c 2o m p L 0 wk . , eH y t ( r m sa d n b l g1 pe d io c r / ll ( m p A m p c 9 m e r w O . o fo . 0 l o a a b o tp c s p fL o li em U i b a I M d o b 1 e s m h 5 c a n r . r 2m r r w u T nc o ocL a a mh U b a a t l r t t m ap nd r i M d oe o o n l c o i ( m 3 A c W i o0 m i h nr n.o c H 9 c o eK r d n p t f o e b pr r i e l e a i R B o e A l o E l t o io s tn e i c yt mt m b p a h ro H i l c m e o n eo so w o a A i o a A nr a h we a n E n oi tc C b l f b p f a c r a w e en ct e ri io g l ro b A e mi ln m n co p o h e C t A b m e d t if b nf l o r c e a e bo E rl w r e e et c H a d o t d i o N OB t hn e p o b pr of h r d l e a o i t c i to l m et b i o s f a K A
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