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296 – Involvement of RANTES promoter gene polymorphisms in the susceptibility of Korean children with asthma
S280
POSTER PRESENTATIONS
OBJECTIVE
STUDY OBJECTIVE
Nitric oxide (NO) plays an immunoregulatory role in balancing cellular immunity. The expression of inducible nitric oxide synthase gene (NOS2) is upregulated upon exposure to proinflammatory cytokines and microbial exposure. Previous studies in Caucasians and Japanese suggested that (CCTTT)n polymorphism in NOS2 promoter confers protection against infections and immunological disorders including atopy. In this study, we investigated the association between (CCTTT)n and asthma traits in Chinese children.
The RANTES (Regulated upon Activation in Normal T cells, Expressed, and Secreted) protein is abundantly expressed during atopic asthma, suggesting an important role in mediation of this disease. The aim of this study was to evaluate the possible role of RANTES promoter polymorphisms in children with asthma.
METHODS Asthmatic children between 5 and 18 years of age and nonallergic controls were recruited from a university teaching hospital. Plasma total and specific IgEs were measured by immunoassays, and exhaled NO concentration (eNO) was quantified online by chemiluminescence. NOS2 (CCTTT)n was genotyped using GeneScan software by ABI-310 gene sequencer. The associations between NOS2 (CCTTT)n and asthma phenotypes were analyzed using multivariate regression, adjusting for age and gender as covariates.
RESULTS The mean (SD) age of 291 asthmatics and 172 controls were 11.1 (3.8) years and 11.6 (4.0) years respectively (p = 0.259). The eNO in 147 patients and 59 controls were 78 (59) ppb and 40 (43) ppb (p < 0.0001). NOS2 (CCTTT)n followed Hardy-Weinberg equilibrium in both groups, and as reported in Caucasians, its uni-modal allele distribution peaks at 12-repeat. Significant inter-ethnic differences in (CCTTT)n alleles were observed, with our Chinese having less 13-repeat (pc = 0.022) but more 17-repeat (pc = 0.033) than Caucasians. The frequency of 14-repeat allele was similar in our Chinese as compared to Japanese (pc = 0.32). Multivariate regression analyses failed to detect any association between this polymorphic marker and asthma diagnosis (p = 0.949), atopy (p = 0.305), IgE sensitization to aeroallergens (p > 0.2 for all) or eNO (p = 0.847).
CONCLUSION These findings do not support NOS2 to be a major candidate gene for asthma, IgE-mediated allergic diseases or eNO in Chinese children. DOI: 10.1016/j.prrv.2006.04.042 A17/296 – Involvement of RANTES promoter gene polymorphisms in the susceptibility of Korean children with asthma Myung Hyun Sohn1, Soo Young Lee2 and Kyu-Earn Kim1 1 Yonsei University, College of Medicine Pediatrics, Seoul, Republic of Korea; 2Ajou University, School of Medicine Pediatrics, Suwon, Republic of Korea
MEASUREMENTS We determined the genotype of 271 children with atopic asthma, 55 children with nonatopic asthma, and 253 control children for allelic determinants at two polymorphic sites in the RANTES promoter region, at positions 403G > A and 28C > G, by restriction fragment length polymorphism (RFLP.)
RESULTS No significant differences were found in genotype and allele frequencies of the RANTES 403G > A and 28C > G polymorphisms between the atopic asthma, nonatopic asthma, and control groups. However, atopic asthmatic patients which were homozygous GG for the RANTES 28C > G exhibited lower PC20 methacholine than those carrying the wild type gene. Additionally, a significantly lower PC20 was demonstrated for the homozygous haplotype 403A/ 28G in asthmatic children.
CONCLUSION The polymorphisms within the RANTES promoter may have a disease-modifying effect in Korean children with asthma. DOI: 10.1016/j.prrv.2006.04.043 A18/301 – Level of nitric oxide in bronchoalveolar lavage fluid of asthmatic mice model F. Fatemi-Nasab1, A. Salek Moghadam1, M. Shabani2 and M. Khakzad1 1 Iran University of Medical Sciences, Department of Immunology, Tehran, Iran; 2Iran University of Medical Sciences, Department of Biochemistry, Tehran, Iran
BACKGROUND Asthma is chronic inflammatory disease with multifactorial and complicated mechanisms. Elevated level of exhaled Nitric Oxide (NO) in asthma and other inflammatory lung disease has led to many studies examining NO as a potential marker of airway inflammation.
OBJECTIVE This study was designed to determine the level of NO in Bronchoalveolar Lavage (BLA) fluid during early and late stages of asthmatic attack in mouse model.
POSTER PRESENTATIONS
OBJECTIVE
STUDY OBJECTIVE
Nitric oxide (NO) plays an immunoregulatory role in balancing cellular immunity. The expression of inducible nitric oxide synthase gene (NOS2) is upregulated upon exposure to proinflammatory cytokines and microbial exposure. Previous studies in Caucasians and Japanese suggested that (CCTTT)n polymorphism in NOS2 promoter confers protection against infections and immunological disorders including atopy. In this study, we investigated the association between (CCTTT)n and asthma traits in Chinese children.
The RANTES (Regulated upon Activation in Normal T cells, Expressed, and Secreted) protein is abundantly expressed during atopic asthma, suggesting an important role in mediation of this disease. The aim of this study was to evaluate the possible role of RANTES promoter polymorphisms in children with asthma.
METHODS Asthmatic children between 5 and 18 years of age and nonallergic controls were recruited from a university teaching hospital. Plasma total and specific IgEs were measured by immunoassays, and exhaled NO concentration (eNO) was quantified online by chemiluminescence. NOS2 (CCTTT)n was genotyped using GeneScan software by ABI-310 gene sequencer. The associations between NOS2 (CCTTT)n and asthma phenotypes were analyzed using multivariate regression, adjusting for age and gender as covariates.
RESULTS The mean (SD) age of 291 asthmatics and 172 controls were 11.1 (3.8) years and 11.6 (4.0) years respectively (p = 0.259). The eNO in 147 patients and 59 controls were 78 (59) ppb and 40 (43) ppb (p < 0.0001). NOS2 (CCTTT)n followed Hardy-Weinberg equilibrium in both groups, and as reported in Caucasians, its uni-modal allele distribution peaks at 12-repeat. Significant inter-ethnic differences in (CCTTT)n alleles were observed, with our Chinese having less 13-repeat (pc = 0.022) but more 17-repeat (pc = 0.033) than Caucasians. The frequency of 14-repeat allele was similar in our Chinese as compared to Japanese (pc = 0.32). Multivariate regression analyses failed to detect any association between this polymorphic marker and asthma diagnosis (p = 0.949), atopy (p = 0.305), IgE sensitization to aeroallergens (p > 0.2 for all) or eNO (p = 0.847).
CONCLUSION These findings do not support NOS2 to be a major candidate gene for asthma, IgE-mediated allergic diseases or eNO in Chinese children. DOI: 10.1016/j.prrv.2006.04.042 A17/296 – Involvement of RANTES promoter gene polymorphisms in the susceptibility of Korean children with asthma Myung Hyun Sohn1, Soo Young Lee2 and Kyu-Earn Kim1 1 Yonsei University, College of Medicine Pediatrics, Seoul, Republic of Korea; 2Ajou University, School of Medicine Pediatrics, Suwon, Republic of Korea
MEASUREMENTS We determined the genotype of 271 children with atopic asthma, 55 children with nonatopic asthma, and 253 control children for allelic determinants at two polymorphic sites in the RANTES promoter region, at positions 403G > A and 28C > G, by restriction fragment length polymorphism (RFLP.)
RESULTS No significant differences were found in genotype and allele frequencies of the RANTES 403G > A and 28C > G polymorphisms between the atopic asthma, nonatopic asthma, and control groups. However, atopic asthmatic patients which were homozygous GG for the RANTES 28C > G exhibited lower PC20 methacholine than those carrying the wild type gene. Additionally, a significantly lower PC20 was demonstrated for the homozygous haplotype 403A/ 28G in asthmatic children.
CONCLUSION The polymorphisms within the RANTES promoter may have a disease-modifying effect in Korean children with asthma. DOI: 10.1016/j.prrv.2006.04.043 A18/301 – Level of nitric oxide in bronchoalveolar lavage fluid of asthmatic mice model F. Fatemi-Nasab1, A. Salek Moghadam1, M. Shabani2 and M. Khakzad1 1 Iran University of Medical Sciences, Department of Immunology, Tehran, Iran; 2Iran University of Medical Sciences, Department of Biochemistry, Tehran, Iran
BACKGROUND Asthma is chronic inflammatory disease with multifactorial and complicated mechanisms. Elevated level of exhaled Nitric Oxide (NO) in asthma and other inflammatory lung disease has led to many studies examining NO as a potential marker of airway inflammation.
OBJECTIVE This study was designed to determine the level of NO in Bronchoalveolar Lavage (BLA) fluid during early and late stages of asthmatic attack in mouse model.