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Abnormal glucose tolerance and insulin resistance in indian polycystic ovary syndrome patients
explore the relationship between sRAGE and PCOS and the relationship between sRAGE and IVF-ET Results in PCOS. DESIGN: Prospective study. MATERIALS AND METHODS: A total of 80 patients of childbearing age were observed and 30 PCOS and 28 non-PCOS women were enrolled into the final analyses for parameters of IVF-ET results. Measurements of sRAGE, VEGF in plasma at early follicular phase and leading follicular fluid aspirated without blood at the time of egg collection by ELISA to examine the difference and the correlation. Parameters of IVF-ET results including the number of the oocyte retrieved, the number of the mature follicles, the normal fertilization rates, and the high-quality embryos. RESULTS: 1 Compared with the non PCOS group, the concentration of sRAGE in plasma and follicular fluid in the PCOS group both were significantly higher (plasma, 1351.79290.62 vs 1185.00205.71, t¼2.148, P¼0.046. follicle fluid, 1650.23239.711 vs 1034.18156.010, t¼11.510, P¼0.000). In PCOS group, the follicular fluid concentration of sRAGE was positively related to the VEGF (r¼0.988, P¼0.000). 2 In PCOS, the concentration of sRAGE in follicular fluid has no relationship with the number of the oocyte retrieved(r¼0.375 ,p¼0.103) and the number of the mature follicles (r¼0.409,P¼0.074), but it was positively related to the IVF-ET parameters including the normal fertilization rates(r¼0.540,P¼0.014), normal cleavage rates(r¼0.489,P¼0.029)and the high-quality embryos(r¼0.539, P¼0.014 ) when the age,BMI,T and LH/FSH were adjusted. CONCLUSIONS: sRAGE may affect the development of inflammatory factor generated in PCOS. The concentration of sRAGE in the follicle fluid was positively related to the IVF-ET parameters,and could be a biomarker to predicate IVF-ET results with PCOS. P-71 Tuesday, October 20, 2015 THE CORRELATION BETWEEN AMH AND LABORATORY PARAMETERS IN PCOS WOMEN ACCORDING TO SUBTYPE: A PILOT-STUDY. Y. Kim, S. Lee, K. Yi, H. Park, J. Shin, T. Kim, J. Hur. Department of Obstetrics and Gynecology, Korea University Guro Hospital, SEOUL, Korea, Republic of. OBJECTIVE: Do AMH level correlate with hormonal and metabolic parameters in women with PCOS according to subtype? DESIGN: A university hospital-based observational study. MATERIALS AND METHODS: The observational study of women with PCOS between January 2013 and November 2014 in University-based medical center. Seventy of women with PCOS were classified according to insulin resistance(IR) by the homeostasis model assessment-estimated insulin resistance(HOMA-IR) or androgen excess(AE) by free androgen index(FAI). The following parameters were analyzed; age, BMI, basal and 2 hour-post prandial(PP2) insulin level, basal and PP2 glucose, HbA1c, testosterone, DHEA, SHBG, AMH, cholesterol, triglyceride, LDL, HDL, and CRP. Between IR(-, n¼33) and IR(+, n¼37), and AE(-, n¼25) and AE(+, n¼45), the correlation between AMH and analyzed parameters were investigated. RESULTS: In overall analysis, AMH had significant positive correlation with SHBG (r¼0.385, P¼ .001), testosterone (r¼0.349, P¼ .004), and HDL (r¼0.340, P¼ .006). In IR (+) type, AMH showed correlation positive with testosterone (r¼0.337, P¼ .041), HDL (r¼0.486, P¼ .003) and negative with CRP (r¼-0.376, P¼ .024), basal and PP2 glucose (r¼-0.343, P¼ .037; r¼-0.347, P¼ .035) whereas not in IR (-) type. In AE (+) type, AMH showed correlation positive with HDL (r¼0.372, P¼ .014) and negative with PP2 glucose (r¼-0.303, P¼ .038) although not in AE (-) type. CONCLUSIONS: AMH may show positive correlation with SHBG and testosterone regardless of PCOS subtype, and have different correlation with metabolic parameters according to IR and AE. To clarification of its clinical meanings, further study with large scale could be necessary.
DESIGN: Cross-sectional study with control group. MATERIALS AND METHODS: 242 patients with PCOS (Rotterdam criteria, 2003) aged 16-34 years and 166 women (control group) aged 1635 years were studied between 01.01.12 and 07.31.14.Prior institutional ethical approval and informed written consent (from all participants/ legal guardians) were obtained. Body mass index (BMI; Kg/M2), abdominal circumference (AC, cm), waist-hip ratio (WHR), Ferriman Galwey score, presence of acne (%) and acanthosis nigricans (AN, %) and blood pressure were assessed. Serum testosterone (T), sex hormone binding globulin (SHBG), post-prandial serum glucose (PPG) and insulin (PPI) levels 2 hours after 75 glucose intake were measured. FAI was calculated as [T (ng/ml) x 100 x 3.47] / SHBG (nmol/l). PPG R 140 mg% was considered as AGT. PPG: PPI % 1.0 was considered as indicative of IR 1. RESULTS: Prevalence of AGT was 11.9% (95% CI ¼ 7.9%, 16.1%) among PCOS patients and 12.6% (95% CI ¼ 7.6%, 17.7%) in the control group (not significantly different). PCOS group was younger and had higher BMI and AC. In regression analysis, after controlling for the covariates, PCOS status was not associated with AGT in our study population; BMI (p¼ 0.011) and WHR (p¼0.027) were the only significant positive predictors (for AGT). Among the PCOS patient population (n¼242), univariate analysis demonstrated that those with IR were significantly younger (p¼0.003) with higher BMI (p<0.001) and AC (p<0.001), and lower occurrence of AN (p<0.001). Multivariate analysis with IR as the dependent outcome failed to identify any significant predictors except age [adjusted Odds Ratio (aOR) ¼ 0.895, 95% CI ¼ 0.825, 0.970]. BMI was borderline significant (aOR ¼ 1.082, 95% CI ¼ 0.994, 1.178). CONCLUSIONS: Our study found no difference in the prevalence of AGT between Indian PCOS and non-PCOS women. We also failed to find any evidence to explain IR in this PCOS patient group by the studied clinical and biochemical parameters. More studies are needed to find out the factors (perhaps environmental factors or dietary habits) other than PCOS status that could be involved in the glucose abnormalities and IR of Indian PCOS women. References: 1. Fritz MA, Speroff L. In Clinical Gynecologic Endocrinology abd Infertility. 8th edition. LWW. Philadelphia. USA. Chapter 13.Page 548.
P-73 Tuesday, October 20, 2015 WITHDRAWN
P-72 Tuesday, October 20, 2015 ABNORMAL GLUCOSE TOLERANCE AND INSULIN RESISTANCE IN INDIAN POLYCYSTIC OVARY SYNDROME PATIENTS. S. M. Bhattacharyaa,b A. Jha.c aObstetrics and Gynecology, KPC Medical College, Kolkata, India; bObstetrics and Gynecology, S.c.das Memorial Medical and Research Center, Kolkata, India; cResearch Associate, West Virginia, WV. OBJECTIVE: To study the prevalence of abnormal glucose tolerance (AGT) and explore the determinants of insulin resistance (IR) in Indian polycystic ovary syndrome (PCOS) patients.
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DESIGN: Cross-sectional study with control group. MATERIALS AND METHODS: 242 patients with PCOS (Rotterdam criteria, 2003) aged 16-34 years and 166 women (control group) aged 1635 years were studied between 01.01.12 and 07.31.14.Prior institutional ethical approval and informed written consent (from all participants/ legal guardians) were obtained. Body mass index (BMI; Kg/M2), abdominal circumference (AC, cm), waist-hip ratio (WHR), Ferriman Galwey score, presence of acne (%) and acanthosis nigricans (AN, %) and blood pressure were assessed. Serum testosterone (T), sex hormone binding globulin (SHBG), post-prandial serum glucose (PPG) and insulin (PPI) levels 2 hours after 75 glucose intake were measured. FAI was calculated as [T (ng/ml) x 100 x 3.47] / SHBG (nmol/l). PPG R 140 mg% was considered as AGT. PPG: PPI % 1.0 was considered as indicative of IR 1. RESULTS: Prevalence of AGT was 11.9% (95% CI ¼ 7.9%, 16.1%) among PCOS patients and 12.6% (95% CI ¼ 7.6%, 17.7%) in the control group (not significantly different). PCOS group was younger and had higher BMI and AC. In regression analysis, after controlling for the covariates, PCOS status was not associated with AGT in our study population; BMI (p¼ 0.011) and WHR (p¼0.027) were the only significant positive predictors (for AGT). Among the PCOS patient population (n¼242), univariate analysis demonstrated that those with IR were significantly younger (p¼0.003) with higher BMI (p<0.001) and AC (p<0.001), and lower occurrence of AN (p<0.001). Multivariate analysis with IR as the dependent outcome failed to identify any significant predictors except age [adjusted Odds Ratio (aOR) ¼ 0.895, 95% CI ¼ 0.825, 0.970]. BMI was borderline significant (aOR ¼ 1.082, 95% CI ¼ 0.994, 1.178). CONCLUSIONS: Our study found no difference in the prevalence of AGT between Indian PCOS and non-PCOS women. We also failed to find any evidence to explain IR in this PCOS patient group by the studied clinical and biochemical parameters. More studies are needed to find out the factors (perhaps environmental factors or dietary habits) other than PCOS status that could be involved in the glucose abnormalities and IR of Indian PCOS women. References: 1. Fritz MA, Speroff L. In Clinical Gynecologic Endocrinology abd Infertility. 8th edition. LWW. Philadelphia. USA. Chapter 13.Page 548.
P-73 Tuesday, October 20, 2015 WITHDRAWN
P-72 Tuesday, October 20, 2015 ABNORMAL GLUCOSE TOLERANCE AND INSULIN RESISTANCE IN INDIAN POLYCYSTIC OVARY SYNDROME PATIENTS. S. M. Bhattacharyaa,b A. Jha.c aObstetrics and Gynecology, KPC Medical College, Kolkata, India; bObstetrics and Gynecology, S.c.das Memorial Medical and Research Center, Kolkata, India; cResearch Associate, West Virginia, WV. OBJECTIVE: To study the prevalence of abnormal glucose tolerance (AGT) and explore the determinants of insulin resistance (IR) in Indian polycystic ovary syndrome (PCOS) patients.
e130
ASRM Abstracts
Vol. 104, No. 3, Supplement, September 2015