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Abstract # 1697 Hair cortisol concentrations associate with hair growth rate
Brain, Behavior, and Immunity 57 (2016) e1–e43
Contents lists available at ScienceDirect
Brain, Behavior, and Immunity journal homepage: www.elsevier.com/locate/ybrbi
Abstracts from the 2016 23rd Annual PNIRS Meeting Abstract # 1697 Hair cortisol concentrations associate with hair growth rate L. Xiang, I. Sunesara, K. Rehm, G.D. Marshall Jr. Univ of MS Med Ctr, Medicine, 2500 N State St, Jackson, MS 39216, United States There is a growing interest in hair cortisol analysis as a valuable biomarker for assessment of chronic stress. However, several methodological variations in hair cortisol analysis raise questions about result validity and equivalency between assays. Those debated points include determining the effects of cosmetic hair treatments and frequency of hair washing on hair cortisol concentrations (HCC), whether HCC remain constant along the length of hair shaft, and the relationship between HCC and hair growth rate. The specific goals of the study were to address these points. Fifty-three normal participants were recruited and hair segments proximal to the scalp were collected from each individual. A cost effective ball mill was used for preparation of hair samples and cortisol levels were measured by ELISA. Results show that frequency of hair washing affects HCC. The group that washed hair everyday had significantly lower HCC than that of not-everyday (p = 0.004). There was no significant difference between cosmetic treated and non-treated hair samples. Study also shows that HCC in the first 3 cm hair segments proximal to the scalp were equivalent with hair growth rate based on 1 cm/month (p = 0.030). Thus, HCC of a 3 cm proximal hair segment may represent cumulative stress exposure over the previous 3 months. These findings will allow more widespread research to validate the utility of HCC as a potential biomarker for assessing chronic stress. http://dx.doi.org/10.1016/j.bbi.2016.07.007
Abstract # 1698 Childhood experience of parental separation and associations with perinatal inflammation E. Fransson a,b, A. Dubicke b, M. Lekander c,d a Center for Health Equity Studies, Karolinska Institutet & Stockholm University, Stockholm 106 91, Sweden b Department for Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden c Stress Research Institute, Stockholm University, Sweden d Department for Clinical Neuroscience, Karolinska Instituet, Stockholm, Sweden
Adversities in childhood are suggested to affect neuroendocrine pathways that in turn increases the risk of elevated inflammation and inflammatory disease in adulthood. During pregnancy, maternal
immune activation has been linked with poor pregnancy outcome and risk for neurodevelopmental disease in the offspring. This study investigates childhood experience of parental separation and current socio economic position in relation to adult immune reaction at the event of giving birth, term or preterm. Sixty-four women were sampled during delivery and post-partum. Serum levels of Interleukin (IL)-6 were analyzed from maternal (preterm n = 27, term n = 37) and cord blood (preterm n = 23, term n = 32). Information on annual income and childhood family situation was collected through questionnaires. Multiple linear regression analyses using log transformed IL-6 data, showed associations between childhood experience of parental separations and elevated levels of maternal IL-6 from labor and post-partum, p < .05 in both preterm and term delivery. Annual income level was negatively associated with maternal labor IL-6 levels as well as with umbilical cord levels; p < .05. The results of this study indicate that early experiences could lead to sensitization of the adult immune system at time of giving birth. Future studies might clarify the importance of these findings regarding inflammation during pregnancy and pregnancy outcomes. http://dx.doi.org/10.1016/j.bbi.2016.07.008
Abstract # 1701 Anti-CD40 prevents the corticosterone-induced loss of splenic dendritic cells in C57BL/6 mice J.L. Yorty Elizabethtown College, Dept. of Biology, One Alpha Drive, Elizabethtown, PA 17022, United States Dendritic cells (DCs) are important immune cells linking the innate and adaptive immune response. DCs capture antigen to present to and activate naïve T cells. Subsets of DCs vary based on protein expression. This research focused on total DCs (CD11c+), and the myeloid and cross-priming DC subsets. Corticosterone (CORT), a glucocorticoid hormone released in response to stress, greatly reduces DC numbers and alters DC function and subsequent T cell responses. The current study quantifies DC recovery after CORT exposure and demonstrates that the loss of DCs can be prevented. C57BL/6 mice were exposed to exogenous CORT in the drinking water for 24 h. The splenic DC populations were then quantified by flow cytometry. CORT exposure significantly decreased the numbers of all splenic DCs. Mice were then provided varying recovery times (3, 5, or 10 days) post CORT exposure. DCs quickly returned to normal levels, and even exceeded the percentages in control untreated mice. Injection of mice with anti-CD40 antibody triggers DC maturation. Thus, it was hypothesized that injection of anti-CD40 may prevent the CORT-induced loss of DCs. Mice received two injections of anti-CD40 antibody and were immediately supplied with CORT
Contents lists available at ScienceDirect
Brain, Behavior, and Immunity journal homepage: www.elsevier.com/locate/ybrbi
Abstracts from the 2016 23rd Annual PNIRS Meeting Abstract # 1697 Hair cortisol concentrations associate with hair growth rate L. Xiang, I. Sunesara, K. Rehm, G.D. Marshall Jr. Univ of MS Med Ctr, Medicine, 2500 N State St, Jackson, MS 39216, United States There is a growing interest in hair cortisol analysis as a valuable biomarker for assessment of chronic stress. However, several methodological variations in hair cortisol analysis raise questions about result validity and equivalency between assays. Those debated points include determining the effects of cosmetic hair treatments and frequency of hair washing on hair cortisol concentrations (HCC), whether HCC remain constant along the length of hair shaft, and the relationship between HCC and hair growth rate. The specific goals of the study were to address these points. Fifty-three normal participants were recruited and hair segments proximal to the scalp were collected from each individual. A cost effective ball mill was used for preparation of hair samples and cortisol levels were measured by ELISA. Results show that frequency of hair washing affects HCC. The group that washed hair everyday had significantly lower HCC than that of not-everyday (p = 0.004). There was no significant difference between cosmetic treated and non-treated hair samples. Study also shows that HCC in the first 3 cm hair segments proximal to the scalp were equivalent with hair growth rate based on 1 cm/month (p = 0.030). Thus, HCC of a 3 cm proximal hair segment may represent cumulative stress exposure over the previous 3 months. These findings will allow more widespread research to validate the utility of HCC as a potential biomarker for assessing chronic stress. http://dx.doi.org/10.1016/j.bbi.2016.07.007
Abstract # 1698 Childhood experience of parental separation and associations with perinatal inflammation E. Fransson a,b, A. Dubicke b, M. Lekander c,d a Center for Health Equity Studies, Karolinska Institutet & Stockholm University, Stockholm 106 91, Sweden b Department for Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden c Stress Research Institute, Stockholm University, Sweden d Department for Clinical Neuroscience, Karolinska Instituet, Stockholm, Sweden
Adversities in childhood are suggested to affect neuroendocrine pathways that in turn increases the risk of elevated inflammation and inflammatory disease in adulthood. During pregnancy, maternal
immune activation has been linked with poor pregnancy outcome and risk for neurodevelopmental disease in the offspring. This study investigates childhood experience of parental separation and current socio economic position in relation to adult immune reaction at the event of giving birth, term or preterm. Sixty-four women were sampled during delivery and post-partum. Serum levels of Interleukin (IL)-6 were analyzed from maternal (preterm n = 27, term n = 37) and cord blood (preterm n = 23, term n = 32). Information on annual income and childhood family situation was collected through questionnaires. Multiple linear regression analyses using log transformed IL-6 data, showed associations between childhood experience of parental separations and elevated levels of maternal IL-6 from labor and post-partum, p < .05 in both preterm and term delivery. Annual income level was negatively associated with maternal labor IL-6 levels as well as with umbilical cord levels; p < .05. The results of this study indicate that early experiences could lead to sensitization of the adult immune system at time of giving birth. Future studies might clarify the importance of these findings regarding inflammation during pregnancy and pregnancy outcomes. http://dx.doi.org/10.1016/j.bbi.2016.07.008
Abstract # 1701 Anti-CD40 prevents the corticosterone-induced loss of splenic dendritic cells in C57BL/6 mice J.L. Yorty Elizabethtown College, Dept. of Biology, One Alpha Drive, Elizabethtown, PA 17022, United States Dendritic cells (DCs) are important immune cells linking the innate and adaptive immune response. DCs capture antigen to present to and activate naïve T cells. Subsets of DCs vary based on protein expression. This research focused on total DCs (CD11c+), and the myeloid and cross-priming DC subsets. Corticosterone (CORT), a glucocorticoid hormone released in response to stress, greatly reduces DC numbers and alters DC function and subsequent T cell responses. The current study quantifies DC recovery after CORT exposure and demonstrates that the loss of DCs can be prevented. C57BL/6 mice were exposed to exogenous CORT in the drinking water for 24 h. The splenic DC populations were then quantified by flow cytometry. CORT exposure significantly decreased the numbers of all splenic DCs. Mice were then provided varying recovery times (3, 5, or 10 days) post CORT exposure. DCs quickly returned to normal levels, and even exceeded the percentages in control untreated mice. Injection of mice with anti-CD40 antibody triggers DC maturation. Thus, it was hypothesized that injection of anti-CD40 may prevent the CORT-induced loss of DCs. Mice received two injections of anti-CD40 antibody and were immediately supplied with CORT