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Abstract 67. Comparison of ELISA assay with hemagglutination inhibition and complement fixation for Subacute Sclerosing Panencephalitis (SSPE) diagnosis
320
Viruses,
immunity
and mental
diseases
labeled compound, 2) high affinity of viral TK for the compound (sensitivity), 3) low affinity of the analogous cellular enzyme (specificity), 4) incorporation into viral DNA, and 5) ready penetration into uninfected brain. Results to date suggest that, using this strategy, selective imaging methods can be developed using positron emission tomography or other scanning techniques in order to permit both early accurate diagnosis and valuable pathophysiological study of human herpes encephalitis.
Abstract 66. Rapid identification of viruses by polarized G.C. Salzman, C.T. Gregg, W.K. Grace and D-M. McGreggor Los Alamos National Laboratory, Los Alamos, U.S.A.
light scattering
measurements
We have developed an instrument that uses the differential scattering of left and right circularly polarized light to distinguish among a variety of viruses including vaccine preparations of four types of dengue fever virus, St. Louis encephalitis, Western equine encephalitis, Eastern equine encephalitis, and Venezuelan equine encephalitis. The approach has also proven successful in distinguishing among a wide variety of bacteria. The method appears to depend on differences in the long range order in the DNA or RNA in the viruses and in the DNA in the bacteria.
Abstract
67.
Comparison of ELISA assay with hemagglutination inhibition and complement fixation for Subacute Sclerosing Panencephalitis (SSPE) diagnosis P. Ferrante, G. Achilli, G. Gerna, F. Bergamini Institute of Virology, University of Milan, Milan, Italy. Virus Laboratory, Institute of Infectious Diseases, Pavia, Italy. Subacute Sclerosing Panencephalitis (SSPE) is a slow, rare and progressive disease of CNS caused by measles virus in young subjects. One of diagnostic criteria is the presence of high levels of measles antibodies in serum and/or in CSF of the patients. In the past years in our laboratories measles antibodies are checked with complement fixing (CF) test and with hemoagglutination inhibition (HI) test. We report the results of the evaluation of the ELISA assay in the diagnosis of SSPE and other neurological diseases in about 30 cases and 27 controls. In SSPE patients the qeometric mean of titers (GMT) in sera was 1:218 with CF, 1:148 with HI and'l:262,636 with ELISA while in controls the GMT was 1:29 with CF 1:19 with HI and 1:7.716 with ELISA. In the CSF of the SSPE cases the GMT was 1:9 in CF, 1:14 with HI and 1:4;298 with ELISA while in the controls the GMT was 1:1.4 1:1.3 and 1:36 respectively. No difference had been observed between the three methods employed regarding the sensibility although the absolute meas antibody titers with ELISA exceeded those determined with the other two techniques tested. However the mode, the range of the antibody titers and the serum/CSF antibody ratios sugg,est that ELISA and CF could be more efficient than HI in the diagnosis of SSPE especially when only the serum is available.
Viruses,
immunity
and mental
diseases
labeled compound, 2) high affinity of viral TK for the compound (sensitivity), 3) low affinity of the analogous cellular enzyme (specificity), 4) incorporation into viral DNA, and 5) ready penetration into uninfected brain. Results to date suggest that, using this strategy, selective imaging methods can be developed using positron emission tomography or other scanning techniques in order to permit both early accurate diagnosis and valuable pathophysiological study of human herpes encephalitis.
Abstract 66. Rapid identification of viruses by polarized G.C. Salzman, C.T. Gregg, W.K. Grace and D-M. McGreggor Los Alamos National Laboratory, Los Alamos, U.S.A.
light scattering
measurements
We have developed an instrument that uses the differential scattering of left and right circularly polarized light to distinguish among a variety of viruses including vaccine preparations of four types of dengue fever virus, St. Louis encephalitis, Western equine encephalitis, Eastern equine encephalitis, and Venezuelan equine encephalitis. The approach has also proven successful in distinguishing among a wide variety of bacteria. The method appears to depend on differences in the long range order in the DNA or RNA in the viruses and in the DNA in the bacteria.
Abstract
67.
Comparison of ELISA assay with hemagglutination inhibition and complement fixation for Subacute Sclerosing Panencephalitis (SSPE) diagnosis P. Ferrante, G. Achilli, G. Gerna, F. Bergamini Institute of Virology, University of Milan, Milan, Italy. Virus Laboratory, Institute of Infectious Diseases, Pavia, Italy. Subacute Sclerosing Panencephalitis (SSPE) is a slow, rare and progressive disease of CNS caused by measles virus in young subjects. One of diagnostic criteria is the presence of high levels of measles antibodies in serum and/or in CSF of the patients. In the past years in our laboratories measles antibodies are checked with complement fixing (CF) test and with hemoagglutination inhibition (HI) test. We report the results of the evaluation of the ELISA assay in the diagnosis of SSPE and other neurological diseases in about 30 cases and 27 controls. In SSPE patients the qeometric mean of titers (GMT) in sera was 1:218 with CF, 1:148 with HI and'l:262,636 with ELISA while in controls the GMT was 1:29 with CF 1:19 with HI and 1:7.716 with ELISA. In the CSF of the SSPE cases the GMT was 1:9 in CF, 1:14 with HI and 1:4;298 with ELISA while in the controls the GMT was 1:1.4 1:1.3 and 1:36 respectively. No difference had been observed between the three methods employed regarding the sensibility although the absolute meas antibody titers with ELISA exceeded those determined with the other two techniques tested. However the mode, the range of the antibody titers and the serum/CSF antibody ratios sugg,est that ELISA and CF could be more efficient than HI in the diagnosis of SSPE especially when only the serum is available.