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Abstract of alkaline phosphatases in fixed plant cells
NOTES
FROM
THE BIOCHEMICAL DR.ELLICE MCDONALD,
RESEARCH
FOUNDATION
DIRXCTOR
Absfract of Sonic Radiation Effects on Rats.-E. D. DAY, D. C. FLETCHER,G. M. NAIMARKAND W. A. MOSHER (J. Aoiation Med., 22, 316(1951)). Sonic radiation of the type emitted most intensely by turbo-jet engines was found to be a stress-producing agent in rats. The desoxypentosenucleic acid content per nucleus of the liver and adrenals was not found to change. No indication was found of secondRats kept in isolation were ary effects of stress due to sonic radiation. found to be less resistant, than rats conditioned by sound and confusion common to most animal rooms, to the lethal effects of sonic radiation. Abstraci of Alkaline Phosphatases in Fixed Plant Cells.-M.
H. Ross AND J. 0. ELY (Experimental Cell Research, 2, 339 (1951)). Alkaline phosphatase activity in fixed plant cells has been demonstrated Presumably cytochemical findings on plant alkaline cytochemically. phosphatases had been lacking because glycerophosphate, which is not hydrolyzed by fixed plant cells, had been used as the substrate. Alkaline phosphatase activity in the onion and corn nuclei has been compared with the activity in rat tissues. In the plant tissues, hydrolysis of phosphates was demonstrated when the substrates guanylic acid, adenosine diphosphate, adenosine triphosphoric acid, diphosphopyridine nucleotide, hexosediphosphates and inorganic pyrophosphate and metaphosphate were used. When the substrates glycerophosphate, adenylic acid and hexosemonophosphates were used, hydrolysis was not found. In the animal tissues however, hydrolysis was demonstrated of all organic phosphoesters employed and of sodium metaphosphate but not the hydrolysis of sodium pyrophosphate. One alkaline phosphatase found in the fixed plant tissues specifically hydrolyzed guanylic acid but no other nucleotide and one specifically hydrolyzed metaphosphate to orthophosphate. The enzymes in both plant and animal cells which hydrolyzed metaphosphates and pyrophosphates were found to require magnesium ions for their activity and to be inhibited by fluoride ions. “Alkaline phosphatase,” so intimately associated with the chromatin in the nucleus, is postulated to be not just one enzyme but a number of enzymes.
FROM
THE BIOCHEMICAL DR.ELLICE MCDONALD,
RESEARCH
FOUNDATION
DIRXCTOR
Absfract of Sonic Radiation Effects on Rats.-E. D. DAY, D. C. FLETCHER,G. M. NAIMARKAND W. A. MOSHER (J. Aoiation Med., 22, 316(1951)). Sonic radiation of the type emitted most intensely by turbo-jet engines was found to be a stress-producing agent in rats. The desoxypentosenucleic acid content per nucleus of the liver and adrenals was not found to change. No indication was found of secondRats kept in isolation were ary effects of stress due to sonic radiation. found to be less resistant, than rats conditioned by sound and confusion common to most animal rooms, to the lethal effects of sonic radiation. Abstraci of Alkaline Phosphatases in Fixed Plant Cells.-M.
H. Ross AND J. 0. ELY (Experimental Cell Research, 2, 339 (1951)). Alkaline phosphatase activity in fixed plant cells has been demonstrated Presumably cytochemical findings on plant alkaline cytochemically. phosphatases had been lacking because glycerophosphate, which is not hydrolyzed by fixed plant cells, had been used as the substrate. Alkaline phosphatase activity in the onion and corn nuclei has been compared with the activity in rat tissues. In the plant tissues, hydrolysis of phosphates was demonstrated when the substrates guanylic acid, adenosine diphosphate, adenosine triphosphoric acid, diphosphopyridine nucleotide, hexosediphosphates and inorganic pyrophosphate and metaphosphate were used. When the substrates glycerophosphate, adenylic acid and hexosemonophosphates were used, hydrolysis was not found. In the animal tissues however, hydrolysis was demonstrated of all organic phosphoesters employed and of sodium metaphosphate but not the hydrolysis of sodium pyrophosphate. One alkaline phosphatase found in the fixed plant tissues specifically hydrolyzed guanylic acid but no other nucleotide and one specifically hydrolyzed metaphosphate to orthophosphate. The enzymes in both plant and animal cells which hydrolyzed metaphosphates and pyrophosphates were found to require magnesium ions for their activity and to be inhibited by fluoride ions. “Alkaline phosphatase,” so intimately associated with the chromatin in the nucleus, is postulated to be not just one enzyme but a number of enzymes.