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Abstract: P485 ALKALOIDS EXTRACTED FROM LOBELIA CHINENSIS LOUR INHIBITED THE FORMATION OF ATHEROSCLEROSIS INDEPENDENT OF LIPID REDUCTION AND APOE PRESENCE
Poster - PHARMACOLOGY OF CVD - Novel Therapies Abstract: P485 Citation: Atherosclerosis Supplement 2009, Vol. 10, Issue 2
ALKALOIDS EXTRACTED FROM LOBELIA CHINENSIS LOUR INHIBITED THE FORMATION OF ATHEROSCLEROSIS INDEPENDENT OF LIPID REDUCTION AND APOE PRESENCE J Wang, W Hu, L Li Institute of Pathophysiology, School of Medicine, Shandong University, Jinan, Shandong Sarafotoxin shows remarkable sequence homology of gene and polypeptide with ET. Earlier studies demonstrated that alkaloids from Lobelia Chinensis Lour (LCLA), an anti-snake venom herbal medicine, could antagonize the bioactive effect of ET-1. Objectives: to examine whether the LCLA could prevent atherosclerosis in apoE-deficient mice. Methods and Results: Male apoE-deficient mice (6 weeks) received normal chow or chow containing LCLA 100 mg/kg/day, simvastatin 50 mg/kg/day. After 16 weeks all the control mice developed atherosclerosis with hypercholesterolemia, while mice treated with LCLA showed much smaller plaque area (lipid core size), despite no changes of plasma cholesterol levels compared to control group. In contrast, mice treated with simvastatin resulted in a paradoxical increase in plasma cholesterol level that aggravate the atherosclerotic lesions. Further studies revealed that LCLA decreased ET-1 levels, ppET-1 expressions, as well as increased eNOS expression in the atherosclerotic plaques, while simvastatin had the opposite effects.
Conclusions: These results suggest that LCLA suppress the atherogenesis in apoE-deficient mice beyond cholesterol lowering, its anti-atherosclerotic effects, differ from simvastatin, are independent of the presence of apoE either.
Funding: National natural science foundation (No. 30800469), Shandong Province Department of Health foundation.
ALKALOIDS EXTRACTED FROM LOBELIA CHINENSIS LOUR INHIBITED THE FORMATION OF ATHEROSCLEROSIS INDEPENDENT OF LIPID REDUCTION AND APOE PRESENCE J Wang, W Hu, L Li Institute of Pathophysiology, School of Medicine, Shandong University, Jinan, Shandong Sarafotoxin shows remarkable sequence homology of gene and polypeptide with ET. Earlier studies demonstrated that alkaloids from Lobelia Chinensis Lour (LCLA), an anti-snake venom herbal medicine, could antagonize the bioactive effect of ET-1. Objectives: to examine whether the LCLA could prevent atherosclerosis in apoE-deficient mice. Methods and Results: Male apoE-deficient mice (6 weeks) received normal chow or chow containing LCLA 100 mg/kg/day, simvastatin 50 mg/kg/day. After 16 weeks all the control mice developed atherosclerosis with hypercholesterolemia, while mice treated with LCLA showed much smaller plaque area (lipid core size), despite no changes of plasma cholesterol levels compared to control group. In contrast, mice treated with simvastatin resulted in a paradoxical increase in plasma cholesterol level that aggravate the atherosclerotic lesions. Further studies revealed that LCLA decreased ET-1 levels, ppET-1 expressions, as well as increased eNOS expression in the atherosclerotic plaques, while simvastatin had the opposite effects.
Conclusions: These results suggest that LCLA suppress the atherogenesis in apoE-deficient mice beyond cholesterol lowering, its anti-atherosclerotic effects, differ from simvastatin, are independent of the presence of apoE either.
Funding: National natural science foundation (No. 30800469), Shandong Province Department of Health foundation.