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AC-022 Evaluation of the pregnancy outcome in three frozen–thawed embryo transfer cycles
Abstracts - Aspire: Optimizing infertility management in Asia
rate, perfect embryos rate and the number of embryos transferred were similar. The implantation rate and pregnancy rate were not significantly different between group A (26.5%, 44.4%) and group B (22.8%, 36.2%), while the implantation rate and pregnancy rate of group C (14.1%, 24.6%) was significantly lower than that of group A (P < 0.01). The implantation rate of group C was significantly lower than that of group B (P < 0.05). The miscarriage rates of group B (15.8%) and group C (18.8%) were significantly lower than that of group A (3.7%, P < 0.05). The ongoing pregnancy rate of group C (20.0%) was significantly lower than that of group A (42.8%, P < 0.01). Group C experienced the lowest implantation rate, pregnancy rate, ongoing pregnancy rate and highest early miscarriage rate. Conclusion: Elevated serum progesterone concentration obtained on the fourth day of stimulation in COH cycles using GnRHa downregulation short protocol results in lower implantation rate, pregnancy rate and higher early miscarriage rate in women undergoing IVF/ ICSI. AC-022 Evaluation of the pregnancy outcome in three frozen–thawed embryo transfer cycles Zong Y1, Gong F, Lu CF, Liu W, Lu GX Institute of Human Reproduction and Stem Cell Engineering, Central South University 1Correspondence: [email protected] Introduction: In frozen–thawed embryo transfer, patients with ovulation failure and abnormal endocrine status are assisted to reproduce by artificial or ovulation induction cycles. We retrospectively investigated patients who accepted the treatment of natural, artificial or ovulation induction cycles to offer a better treatment approach. Materials/Methods: This retrospective study analysed 121 frozen embryo transfers carried out during January to December of 2005 in the Reproductive and Genetic hospital of CITIC-Xiangya, including 73 natural cycles (group 1), 22 artificial cycles (group 2) and 26 ovulation induction cycles (group 3). The ages, weight index (WI), luteinizing hormone (LH)/follicle-stimulating hormone (FSH) ratios, embryo transfer numbers and pregnancy rates of these three groups were compared respectively. Results: There were no significant differences for ages, WI and embryo transfer numbers in the three groups (P > 0.05). LH/FSH ratios of group 2 (1.94 ± 1.77) and group 3 (0.99 ± 0.49) were higher than that in group 1 (0.74 ± 0.43, P = 0.02 and 0.00, <0.05). The pregnancy rates of group 1, group 2 and group 3 were 56.2%, 59.1% and 42.3%, which had no remarkable difference (P = 0.408, P > 0.05). Conclusion: For embryo-transferred patients of ovulation failure or abnormal endocrine levels, both artificial and ovulation induction cycles could produce satisfactory pregnancy outcomes. AC-023 Forecasting the value of endometrial oestrogen receptor, progesterone receptor and vascular endothelial growth factor in the window of implantation on pregnancy results of IVF and embryo transfer Tan L1, Dong FL, Zheng Y, Zuo Y Reproductive Medical Centre, The Second Affiliated Hospital, Zhengzhou University 1Correspondence: [email protected] Objective: To study the relationship of endometrial oestrogen receptor (ER), progesterone receptor (PR) and vascular endothelial growth factor (VEGF) in the window of implantation and pregnancy results of IVF and embryo transfer (IVF–ET), and to select the indices that can be used to predict IVF–ET results. Materials/Methods: The endometrium tissue samples were obtained from 40 patients undergoing IVF–ET in the window of implantation. The endometrial ER, PR and VEGF were detected by immunohistochemistry using monoclonal antibody and semi-quantified by using the H-score. Forty patients were divided into two groups: the
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pregnancy group (16 patients) and non-pregnancy group (24 patients). Results: The endometrial luminal epithelial and glandular epithelial PR score in the non-pregnancy group was significantly higher than that in the pregnancy group (P < 0.05). The endometrial stromal PR score in the non-pregnancy group was significantly lower than that in the pregnancy group (P < 0.05). The endometrial ER score was not significantly different in the two groups. The endometrial immunostaining VEGF score was higher in the pregnancy group than that in the non-pregnancy group (P < 0.05). Conclusion: The failure of down-regulation of the endometrial luminal epithelial, glandular epithelial PR, decreased stromal PR and the low expressions of endometrial VEGF in the window of implantation can result in IVF–ET failure. The expressions of endometrial PR and VEGF in the window of implantation can be used to predict IVF-ET results. AC-024 Increased serum progesterone/oestradiol ratio in the late follicular phase could be related to lower clinical pregnancy rate in in-vitro fertilization programme Lai TH1,2,3, Chen SC1 1Department of Obstetrics and Gynecology, Cathay General Hospital; 2Department of Medicine, Catholic Fu Jen University 3Correspondence: [email protected] Introduction: Premature luteinization occurring in the late follicular phase of the IVF cycle could be associated with poor oocyte maturation, a decreased fertilization rate and impaired clinical pregnancy rate. The aim of this study was to evaluate whether increased progesterone/ oestradiol ratio could be an index of premature luteinization and related to lower clinical pregnancy rates in women undergoing IVF–embryo transfer (IVF–ET). Materials/Methods: From March to November 2003, 76 consecutive infertile women who received IVF–ET treatment with the long GnRHa protocol at Cathay General Hospital were enrolled in this study. Women were divided into those with (study group) or without premature luteinization (control group). Premature luteinization was defined as progesterone (P) s 1,000/oestradiol >1 on the day of HCG administration. Down-regulation was achieved after administration of GnRHa (buserelin acetate 900 Mg/day) and assured by serum oestradiol levels less than 50 pg/mL on day 3 of a treatment cycle. Ovulation induction started with human menopausal gonadotrophin (HMG, Pergonal) 225 IU per day for day 3 to day 5 and with folliclestimulating hormone (FSH, Gonal-f) 225 IU per day for day 3 to day 5, and then 300 IU per day for day 6 to day 10. Gonadotrophin dosage in each patient was tailored in accordance with follicular sizes by transvaginal ultrasound scanning and serum oestradiol level. When there were three or more follicles with diameter up to 18 mm, 10,000 IU of HCG was administered. Serum luteinizing hormone (LH) level was evaluated on HCG administration day. Clinical pregnancy rate per cycle was compared by Student’s t-test and chi-squared test; P < 0.05 was considered statistically significant. Results: The characteristics of the patients were similar between the study and the control groups. Late follicular progesterone s 1000/ oestradiol ratio was significantly higher in the study as compared with the control group, 1.8 ± 0.8 and 0.5 ± 0.3, respectively (P < 0.05). The clinical pregnancy rate per cycle was significantly lower in the study group as opposed to controls, 13.6% and 29.6%, respectively (P < 0.05). Ovarian reserve parameters including day 3 FSH, total amount of HMG, number of follicles, oocytes and embryos did not differ between the two groups. Progesterone levels on HCG day and day 3 LH levels were significantly superior in the study group as compared with the control group, 1.5 ± 1.1 and 0.7 ± 0.5 (ng/mL); 5.8 ± 8.4 and 2.9 ± 2.2 (mIU/mL), respectively (P < 0.05). However, oestradiol levels on HCG day were significantly inferior in the study group compared with the control group, 934.3 ± 556.4 and 1800.6 ± 1,227.2 (pg/mL), respectively (P < 0.05). Conclusion: Increased serum progesterone/oestradiol ratio in the late follicular phase could adversely affect the clinical pregnancy rate in IVF–ET cycles. Progesterone/oestradiol ratio could be an index of premature luteinization to predict the outcome of pregnancy.
rate, perfect embryos rate and the number of embryos transferred were similar. The implantation rate and pregnancy rate were not significantly different between group A (26.5%, 44.4%) and group B (22.8%, 36.2%), while the implantation rate and pregnancy rate of group C (14.1%, 24.6%) was significantly lower than that of group A (P < 0.01). The implantation rate of group C was significantly lower than that of group B (P < 0.05). The miscarriage rates of group B (15.8%) and group C (18.8%) were significantly lower than that of group A (3.7%, P < 0.05). The ongoing pregnancy rate of group C (20.0%) was significantly lower than that of group A (42.8%, P < 0.01). Group C experienced the lowest implantation rate, pregnancy rate, ongoing pregnancy rate and highest early miscarriage rate. Conclusion: Elevated serum progesterone concentration obtained on the fourth day of stimulation in COH cycles using GnRHa downregulation short protocol results in lower implantation rate, pregnancy rate and higher early miscarriage rate in women undergoing IVF/ ICSI. AC-022 Evaluation of the pregnancy outcome in three frozen–thawed embryo transfer cycles Zong Y1, Gong F, Lu CF, Liu W, Lu GX Institute of Human Reproduction and Stem Cell Engineering, Central South University 1Correspondence: [email protected] Introduction: In frozen–thawed embryo transfer, patients with ovulation failure and abnormal endocrine status are assisted to reproduce by artificial or ovulation induction cycles. We retrospectively investigated patients who accepted the treatment of natural, artificial or ovulation induction cycles to offer a better treatment approach. Materials/Methods: This retrospective study analysed 121 frozen embryo transfers carried out during January to December of 2005 in the Reproductive and Genetic hospital of CITIC-Xiangya, including 73 natural cycles (group 1), 22 artificial cycles (group 2) and 26 ovulation induction cycles (group 3). The ages, weight index (WI), luteinizing hormone (LH)/follicle-stimulating hormone (FSH) ratios, embryo transfer numbers and pregnancy rates of these three groups were compared respectively. Results: There were no significant differences for ages, WI and embryo transfer numbers in the three groups (P > 0.05). LH/FSH ratios of group 2 (1.94 ± 1.77) and group 3 (0.99 ± 0.49) were higher than that in group 1 (0.74 ± 0.43, P = 0.02 and 0.00, <0.05). The pregnancy rates of group 1, group 2 and group 3 were 56.2%, 59.1% and 42.3%, which had no remarkable difference (P = 0.408, P > 0.05). Conclusion: For embryo-transferred patients of ovulation failure or abnormal endocrine levels, both artificial and ovulation induction cycles could produce satisfactory pregnancy outcomes. AC-023 Forecasting the value of endometrial oestrogen receptor, progesterone receptor and vascular endothelial growth factor in the window of implantation on pregnancy results of IVF and embryo transfer Tan L1, Dong FL, Zheng Y, Zuo Y Reproductive Medical Centre, The Second Affiliated Hospital, Zhengzhou University 1Correspondence: [email protected] Objective: To study the relationship of endometrial oestrogen receptor (ER), progesterone receptor (PR) and vascular endothelial growth factor (VEGF) in the window of implantation and pregnancy results of IVF and embryo transfer (IVF–ET), and to select the indices that can be used to predict IVF–ET results. Materials/Methods: The endometrium tissue samples were obtained from 40 patients undergoing IVF–ET in the window of implantation. The endometrial ER, PR and VEGF were detected by immunohistochemistry using monoclonal antibody and semi-quantified by using the H-score. Forty patients were divided into two groups: the
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pregnancy group (16 patients) and non-pregnancy group (24 patients). Results: The endometrial luminal epithelial and glandular epithelial PR score in the non-pregnancy group was significantly higher than that in the pregnancy group (P < 0.05). The endometrial stromal PR score in the non-pregnancy group was significantly lower than that in the pregnancy group (P < 0.05). The endometrial ER score was not significantly different in the two groups. The endometrial immunostaining VEGF score was higher in the pregnancy group than that in the non-pregnancy group (P < 0.05). Conclusion: The failure of down-regulation of the endometrial luminal epithelial, glandular epithelial PR, decreased stromal PR and the low expressions of endometrial VEGF in the window of implantation can result in IVF–ET failure. The expressions of endometrial PR and VEGF in the window of implantation can be used to predict IVF-ET results. AC-024 Increased serum progesterone/oestradiol ratio in the late follicular phase could be related to lower clinical pregnancy rate in in-vitro fertilization programme Lai TH1,2,3, Chen SC1 1Department of Obstetrics and Gynecology, Cathay General Hospital; 2Department of Medicine, Catholic Fu Jen University 3Correspondence: [email protected] Introduction: Premature luteinization occurring in the late follicular phase of the IVF cycle could be associated with poor oocyte maturation, a decreased fertilization rate and impaired clinical pregnancy rate. The aim of this study was to evaluate whether increased progesterone/ oestradiol ratio could be an index of premature luteinization and related to lower clinical pregnancy rates in women undergoing IVF–embryo transfer (IVF–ET). Materials/Methods: From March to November 2003, 76 consecutive infertile women who received IVF–ET treatment with the long GnRHa protocol at Cathay General Hospital were enrolled in this study. Women were divided into those with (study group) or without premature luteinization (control group). Premature luteinization was defined as progesterone (P) s 1,000/oestradiol >1 on the day of HCG administration. Down-regulation was achieved after administration of GnRHa (buserelin acetate 900 Mg/day) and assured by serum oestradiol levels less than 50 pg/mL on day 3 of a treatment cycle. Ovulation induction started with human menopausal gonadotrophin (HMG, Pergonal) 225 IU per day for day 3 to day 5 and with folliclestimulating hormone (FSH, Gonal-f) 225 IU per day for day 3 to day 5, and then 300 IU per day for day 6 to day 10. Gonadotrophin dosage in each patient was tailored in accordance with follicular sizes by transvaginal ultrasound scanning and serum oestradiol level. When there were three or more follicles with diameter up to 18 mm, 10,000 IU of HCG was administered. Serum luteinizing hormone (LH) level was evaluated on HCG administration day. Clinical pregnancy rate per cycle was compared by Student’s t-test and chi-squared test; P < 0.05 was considered statistically significant. Results: The characteristics of the patients were similar between the study and the control groups. Late follicular progesterone s 1000/ oestradiol ratio was significantly higher in the study as compared with the control group, 1.8 ± 0.8 and 0.5 ± 0.3, respectively (P < 0.05). The clinical pregnancy rate per cycle was significantly lower in the study group as opposed to controls, 13.6% and 29.6%, respectively (P < 0.05). Ovarian reserve parameters including day 3 FSH, total amount of HMG, number of follicles, oocytes and embryos did not differ between the two groups. Progesterone levels on HCG day and day 3 LH levels were significantly superior in the study group as compared with the control group, 1.5 ± 1.1 and 0.7 ± 0.5 (ng/mL); 5.8 ± 8.4 and 2.9 ± 2.2 (mIU/mL), respectively (P < 0.05). However, oestradiol levels on HCG day were significantly inferior in the study group compared with the control group, 934.3 ± 556.4 and 1800.6 ± 1,227.2 (pg/mL), respectively (P < 0.05). Conclusion: Increased serum progesterone/oestradiol ratio in the late follicular phase could adversely affect the clinical pregnancy rate in IVF–ET cycles. Progesterone/oestradiol ratio could be an index of premature luteinization to predict the outcome of pregnancy.