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Achievement of a biofuel-like biodiesel by regioselective transesterification of sunflower oil with mucor miehei lipase
New Biotechnology · Volume 31S · July 2014
can have the desired effect of increased carbon flux towards 3-hydroxybutyrate. http://dx.doi.org/10.1016/j.nbt.2014.05.1833
BIOFUELS, BIOCHEMICALS AND BIOENERGY
PB-08 The usage of carrot pomace as a feedstock for bioethanol production Ekin Demiray ∗ , Sevgi Ertugrul Karatay, Gönül Dönmez, Sedat Dönmez
PB-07 Achievement of a biofuel-like biodiesel by regioselective transesterification of sunflower oil with mucor miehei lipase Juan Calero 1,∗ , Juan Calero 2 , Diego Luna 2 , Enrique D. Sancho 3 , Carlos Luna 2 , Cristóbal Verdugo 4 , Alejandro Posadillo 5 , Felipa M. Bautista 2 , Antonio A. Romero 2 1
University of Cordoba Department of Organic Chemistry, University of Córdoba 3 Department of Microbiology, University of Córdoba 4 Crystallographic Studies Laboratory, Andalusian Institute of Earth Sciences, CSIC 5 Seneca Green Catalyst S.L 2
In previous researches, we have developed a biofuel that avoid the production of glycerol as by-product. This biofuel is similar to the conventional biodiesel, being in the same way applicable to diesel engines. Thus, glycerol is kept as monoglyceride (MG), together to two fatty acid ethyl esters (FAEE) molecules. In this respect, this biofuel is obtained by a partial ethanolysis of sunflower oil with Mucor Miehei lipase as biocatalyst. Results obtained by using M Miehei lipase have shown that this lipase is an efficient biocatalyst in the 1,3-selective enzymatic ethanolysis reaction of triglycerides. Thus, reactions were performed to determine the optimal conditions, such as amount of lipase, volume of NaOH 10 N aqueous solution, temperature and oil/ethanol molar ratio. It was always used 12 mL of sunflower oil, in a 25 mL round bottom flask, with a conventional magnetic stirrer at 300 rpm, during 2 h. Finally, a study of reuses is also carried out. The optimized conditions obtained were 3.5 mL of absolute ethanol (oil/ethanol molar ratio 1/6), 37.5 l of NaOH 10 N solution, temperature of 30 ◦ C and 15 mg of M Miehei lipase. Operating under these experimental conditions, eighteen successive reactions were efficiently carried out after recovering the lipases by centrifugation. Acknowledgements Grants from the Spanish Ministry of Economy and Competitiveness (Project ENE 2011-27017), Spanish Ministry of Education and Science (Projects CTQ2010-18126 and CTQ201128954-C02-02), FEDER funds and Junta de Andalucía FQM 0191, PO8-RMN-03515 and P11-TEP-7723. http://dx.doi.org/10.1016/j.nbt.2014.05.1834
Ankara University
Keywords: Bioethanol; Saccharomyces cerevisiae In today’s world there is urgent need for alternative energy sources due to rapid depletion of the fossil fuels. Renewable sources are good candidates instead of fossil fuels because of their environmentally friendly and less toxic properties. It has been estimated that bioethanol will be the most widely used renewable source in the near future. The usage of agricultural wastes for bioethanol production has some advantages such as lower production costs. Therefore in this study we investigated the potential of carrot pomaces as a feedstock for bioethanol production by using Saccharomyces cerevisiae. For obtaining fermentable sugars carrot pomaces were hydrolysed in 1.5% H2 SO4 (v/v). The yeast growth, initial and consumed sugar concentrations were monitored periodically. The bioethanol concentration was determinated with gas chromotography. The microbial growth media containing carrot pomace as a carbon source and different nitrogen sources were prepared to increase the bioethanol production. It has been observed that yeast cells used 35.4 g/L sugar in the medium containing 0.5 g/L KH2 PO4 and1 g/L (NH4 )2 SO4 . As a result the media that were prepared with carrot pomace sugars supported the growth of Saccharomyces cerevisiae. These results show that carrot pomaces are suitable feedstocks for bioethanol production. http://dx.doi.org/10.1016/j.nbt.2014.05.1835
PB-09 Lactic acid production from lignocellulosic hydrolysates under non-sterilized conditions using Bacillus coagulans IPE22 Yinhua Wan ∗ , Yuming Zhang, Xiangrong Chen, Benkun Qi, Yi Su Institute of Process Engineering, Chinese Academy of Sciences
A thermophilic lactic acid (LA) producer was isolated and identified as Bacillus coagulans strain IPE22. The strain showed remarkable capability to ferment pentose, hexose and cellobiose, and was also resistant to inhibitors from lignocellulosic hydrolysates. Based on the strain’s promising features, it was used to produce lactic acid (LA) from mixed sugar and wheat straw hydrolysates under non-sterilized conditions. In order to eliminate the sequential utilization of mixed sugar and feedback inhibition during batch fermentation, membrane integrated repeated batch fermentation (MIRB) was used to improve LA productivity. With MIRB, a high cell density was obtained and the simultaneous fermentation of glucose, xylose and arabinose was successfully realized. The separation of LA from broth by membrane in batch fermentation also decreased feedback inhibition. MIRB was carried www.elsevier.com/locate/nbt S95
can have the desired effect of increased carbon flux towards 3-hydroxybutyrate. http://dx.doi.org/10.1016/j.nbt.2014.05.1833
BIOFUELS, BIOCHEMICALS AND BIOENERGY
PB-08 The usage of carrot pomace as a feedstock for bioethanol production Ekin Demiray ∗ , Sevgi Ertugrul Karatay, Gönül Dönmez, Sedat Dönmez
PB-07 Achievement of a biofuel-like biodiesel by regioselective transesterification of sunflower oil with mucor miehei lipase Juan Calero 1,∗ , Juan Calero 2 , Diego Luna 2 , Enrique D. Sancho 3 , Carlos Luna 2 , Cristóbal Verdugo 4 , Alejandro Posadillo 5 , Felipa M. Bautista 2 , Antonio A. Romero 2 1
University of Cordoba Department of Organic Chemistry, University of Córdoba 3 Department of Microbiology, University of Córdoba 4 Crystallographic Studies Laboratory, Andalusian Institute of Earth Sciences, CSIC 5 Seneca Green Catalyst S.L 2
In previous researches, we have developed a biofuel that avoid the production of glycerol as by-product. This biofuel is similar to the conventional biodiesel, being in the same way applicable to diesel engines. Thus, glycerol is kept as monoglyceride (MG), together to two fatty acid ethyl esters (FAEE) molecules. In this respect, this biofuel is obtained by a partial ethanolysis of sunflower oil with Mucor Miehei lipase as biocatalyst. Results obtained by using M Miehei lipase have shown that this lipase is an efficient biocatalyst in the 1,3-selective enzymatic ethanolysis reaction of triglycerides. Thus, reactions were performed to determine the optimal conditions, such as amount of lipase, volume of NaOH 10 N aqueous solution, temperature and oil/ethanol molar ratio. It was always used 12 mL of sunflower oil, in a 25 mL round bottom flask, with a conventional magnetic stirrer at 300 rpm, during 2 h. Finally, a study of reuses is also carried out. The optimized conditions obtained were 3.5 mL of absolute ethanol (oil/ethanol molar ratio 1/6), 37.5 l of NaOH 10 N solution, temperature of 30 ◦ C and 15 mg of M Miehei lipase. Operating under these experimental conditions, eighteen successive reactions were efficiently carried out after recovering the lipases by centrifugation. Acknowledgements Grants from the Spanish Ministry of Economy and Competitiveness (Project ENE 2011-27017), Spanish Ministry of Education and Science (Projects CTQ2010-18126 and CTQ201128954-C02-02), FEDER funds and Junta de Andalucía FQM 0191, PO8-RMN-03515 and P11-TEP-7723. http://dx.doi.org/10.1016/j.nbt.2014.05.1834
Ankara University
Keywords: Bioethanol; Saccharomyces cerevisiae In today’s world there is urgent need for alternative energy sources due to rapid depletion of the fossil fuels. Renewable sources are good candidates instead of fossil fuels because of their environmentally friendly and less toxic properties. It has been estimated that bioethanol will be the most widely used renewable source in the near future. The usage of agricultural wastes for bioethanol production has some advantages such as lower production costs. Therefore in this study we investigated the potential of carrot pomaces as a feedstock for bioethanol production by using Saccharomyces cerevisiae. For obtaining fermentable sugars carrot pomaces were hydrolysed in 1.5% H2 SO4 (v/v). The yeast growth, initial and consumed sugar concentrations were monitored periodically. The bioethanol concentration was determinated with gas chromotography. The microbial growth media containing carrot pomace as a carbon source and different nitrogen sources were prepared to increase the bioethanol production. It has been observed that yeast cells used 35.4 g/L sugar in the medium containing 0.5 g/L KH2 PO4 and1 g/L (NH4 )2 SO4 . As a result the media that were prepared with carrot pomace sugars supported the growth of Saccharomyces cerevisiae. These results show that carrot pomaces are suitable feedstocks for bioethanol production. http://dx.doi.org/10.1016/j.nbt.2014.05.1835
PB-09 Lactic acid production from lignocellulosic hydrolysates under non-sterilized conditions using Bacillus coagulans IPE22 Yinhua Wan ∗ , Yuming Zhang, Xiangrong Chen, Benkun Qi, Yi Su Institute of Process Engineering, Chinese Academy of Sciences
A thermophilic lactic acid (LA) producer was isolated and identified as Bacillus coagulans strain IPE22. The strain showed remarkable capability to ferment pentose, hexose and cellobiose, and was also resistant to inhibitors from lignocellulosic hydrolysates. Based on the strain’s promising features, it was used to produce lactic acid (LA) from mixed sugar and wheat straw hydrolysates under non-sterilized conditions. In order to eliminate the sequential utilization of mixed sugar and feedback inhibition during batch fermentation, membrane integrated repeated batch fermentation (MIRB) was used to improve LA productivity. With MIRB, a high cell density was obtained and the simultaneous fermentation of glucose, xylose and arabinose was successfully realized. The separation of LA from broth by membrane in batch fermentation also decreased feedback inhibition. MIRB was carried www.elsevier.com/locate/nbt S95