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Activin a inhibits apoptosis and induces acquisition of EMT phenotypes in normal keratinocytes
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Abstracts
VEGF RECEPTOR IS ASSOCIATED WITH EPITHELIAL DYSPLASIA GRADING Fraga CAC1, Oliveira MVM1, Gomez RS2, De Paula AM1, Guimaraes ALS1 - 1DEPARTMENT OF DENTISTRY, - UNIVERSIDADE ESTADUAL DE MONTES CLAROS, MONTES CLAROS, BRAZIL, 2DEPARTMENT OF CLINICAL, SURGERY AND ORAL PATHOLOGY, - SCHOOL OF DENTISTRY, UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, BRAZIL Objectives: The present study aimed to identify whether there are alterations in the levels of VEGFR2 and MMP-9 in epithelial dysplasia grading. Study design: This retrospective study was performed on archived tissue blocks 48 patients with ED and 20 patients with OSCC and 6 mucoceles biopsies were used as a control group. Immunohistochemistry of VEGFR2 and MMP9 were performed and compared among groups. Results: VEGFR2 and MMP9 immunoexpression appeared to correlate with the degree of dysplasia and was observed to increase in lesions with more severe dysplasia as compared to those with lower degrees of dysplasia. Immunoreactivity of MMP-9 was lower in the ED samples compared to the OSCC samples (p ⫽ 0.004). We observed no difference in VEGFR2 protein levels between ED and OSCC samples. Conclusion: Our data indicate that an increase in the levels of both VEGFR2 and MMP-9 is associated with worse ED grading.
SQUAMOUS CELL CARCINOMA OF THE LIP: CLINICAL PROFILE OF PATIENTS AND HISTOLOGICAL GRADING OF LESIONS IN A REFERENCE CENTER OF AMAZONAS Castro AS1, Pereira FRS1, Lopes MSP1, Almeida NS2, Camara J2, Santos PJB3, Libório TN2 - 1FEDERAL UNIVERSITY OF AMAZONAS - SCHOOL OF DENTISTRY, 2FEDERAL UNIVERSITY OF AMAZONAS - PATHOLOGY AND LEGAL MEDICINE, 3ONCOLOGY CENTER CONTROL FOUNDATION OF THE AMAZONAS (FCECON) Objective: This study aimed to evaluate the clinical profile and histological grading of SCC of the lip (LSCC). Study design: It was a retrospective study of patients over 40 years old treated in a Reference Center of Oncology in Amazonas from 2002 to 2011. Clinical data were collected from medical records and a review of histological sections was performed. Results: Nineteen patients with LSCC were found, 52.6% of them were female, 68.4% had over 60 years old, 63,1% was represented by a mixed race called “pardo”, 84,2% didn’t report alcohol consumption and just 12.5% reported tobacco use. Well differentiated LSCC was the most common histological grading (52,63%), especially in lower lip (57,8%). Status of Linfonodal involvement and metastasis were not informed in most cases (84,2%). Conclusion: In the North of Brazil, data of LSCC are scarce, probably due to the lack of studies and also due to the sub notification.
ACTIVIN A INHIBITS APOPTOSIS AND INDUCES ACQUISITION OF EMT PHENOTYPES IN NORMAL KERATINOCYTES Bufalino A1, Rodrigues PC1, Bastos DC1, Sammartino F1, Graner E1, Coletta RD1 - 1PIRACICABA
October 2012 DENTAL SCHOOL, UNIVERSITY OF CAMPINAS (UNICAMP) - ORAL DIAGNOSIS Objective: The purpose of this study was to investigate the effects of activin A, a member of TGF superfamily that has been shown to be overexpressed in various cancers, in the modulation of the key events associated with oral tumorigenesis, including proliferation, apoptosis and epithelial-mesenchymal transition (EMT). Study design: To assess the effects of activin A, the normal epithelial cell line HaCAT was cultured in medium containing 0-100 ng/ml of recombinant activin A. Results: Our results demonstrated that activin A promotes a dose-dependent decreased of apoptosis and death cell, with a significant inhibition on cell proliferation only at 100 ng/ml as revealed by cell cycle analysis and BrdU-labeling index. Additionally, activin A treatment stimulated significantly the expression of vimentin and N-cadherin, while reduced E-cadherin and -catenin expression. Conclusion: These findings demonstrate that activin A modulates apoptosis and acquisition of EMT phenotypes, contributing to oral tumorigenesis.
ONCOCYTIC METAPLASIA IN ORAL MYOEPITHELIOMA: REPORT OF A CASE Correia AL1, Gueiros Lam1, Leão JC1, Castro JFL1, León JE2, Almeida OP3, Perez DEC1 1 UNIVERSIDADE FEDERAL DE PERNAMBUCO - PROGRAMA DE PÓS GRADUAÇÃO EM ODONTOLOGIA, 2 UNIVERSIDADE DE SÃO PAULO - FACULDADE DE ODONTOLOGIA DE RIBEIRÃO PRETO, 3UNICAMP FACULDADE DE ODONTOLOGIA DE PIRACICABA The occurrence of oncocytic metaplasia in salivary gland tumors is a relatively common finding. However, depending on the extension, these areas may lead to misdiagnosis. The aim of this report is to describe a case of myoepithelioma with oncocytic metaplasia. A 40-year-old female presented a painless swelling in the hard palate with 4 years of evolution. Intraoral examination revealed a nodular lesion, with firm consistency and covered by normal color oral mucosa. A salivary gland tumor was the main hypothesis of diagnosis. Microscopically, the lesion was composed by epithelioid cells, which were arranged in nests and blocks. Several cells presented a granular, eosinophilic and enlargement cytoplasm, similar to oncocytic cells. The tumor cells were positive for AE1/AE3, cytokeratin 8 and p63. The diagnosis of myoepithelioma with oncocytic metaplasia was established. The patient was submitted to surgical excision of the lesion. Currently, the patient is under periodical follow up.
LIPOSARCOMA OF THE TONGUE: CASE REPORT Torrezani A1, Lima AN2, Esteves CMD3, Martins FM3, Nunes FD3, Milani B. de A2, Jorge WA2 - 1UNIVERSITY OF SÃO PAULO - STOMATOLOGY, 2MUNICIPAL HOSPITAL OF CAMPO LIMPO - MAXILLOFACIAL SURGERY, 3UNIVERSITY OF SÃO PAULO - ORAL PATHOLOGY Liposarcoma is a rare mesenchymal malignancy and affects more men, between the 4th and 6th decade of life. A 21-years-old male with a 4 year duration, asymptomatic swelling, on the dorsum of the tongue present himself for diagnosis. The clinical examination showed an approximately 4 cm nodule in diameter with ulcerated surface, erythroplasia, telangiectasia and a firm consistence. An incisional biopsy was made and the histopathological findings revealed a proliferation of round cells with hy-
e46
Abstracts
VEGF RECEPTOR IS ASSOCIATED WITH EPITHELIAL DYSPLASIA GRADING Fraga CAC1, Oliveira MVM1, Gomez RS2, De Paula AM1, Guimaraes ALS1 - 1DEPARTMENT OF DENTISTRY, - UNIVERSIDADE ESTADUAL DE MONTES CLAROS, MONTES CLAROS, BRAZIL, 2DEPARTMENT OF CLINICAL, SURGERY AND ORAL PATHOLOGY, - SCHOOL OF DENTISTRY, UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, BRAZIL Objectives: The present study aimed to identify whether there are alterations in the levels of VEGFR2 and MMP-9 in epithelial dysplasia grading. Study design: This retrospective study was performed on archived tissue blocks 48 patients with ED and 20 patients with OSCC and 6 mucoceles biopsies were used as a control group. Immunohistochemistry of VEGFR2 and MMP9 were performed and compared among groups. Results: VEGFR2 and MMP9 immunoexpression appeared to correlate with the degree of dysplasia and was observed to increase in lesions with more severe dysplasia as compared to those with lower degrees of dysplasia. Immunoreactivity of MMP-9 was lower in the ED samples compared to the OSCC samples (p ⫽ 0.004). We observed no difference in VEGFR2 protein levels between ED and OSCC samples. Conclusion: Our data indicate that an increase in the levels of both VEGFR2 and MMP-9 is associated with worse ED grading.
SQUAMOUS CELL CARCINOMA OF THE LIP: CLINICAL PROFILE OF PATIENTS AND HISTOLOGICAL GRADING OF LESIONS IN A REFERENCE CENTER OF AMAZONAS Castro AS1, Pereira FRS1, Lopes MSP1, Almeida NS2, Camara J2, Santos PJB3, Libório TN2 - 1FEDERAL UNIVERSITY OF AMAZONAS - SCHOOL OF DENTISTRY, 2FEDERAL UNIVERSITY OF AMAZONAS - PATHOLOGY AND LEGAL MEDICINE, 3ONCOLOGY CENTER CONTROL FOUNDATION OF THE AMAZONAS (FCECON) Objective: This study aimed to evaluate the clinical profile and histological grading of SCC of the lip (LSCC). Study design: It was a retrospective study of patients over 40 years old treated in a Reference Center of Oncology in Amazonas from 2002 to 2011. Clinical data were collected from medical records and a review of histological sections was performed. Results: Nineteen patients with LSCC were found, 52.6% of them were female, 68.4% had over 60 years old, 63,1% was represented by a mixed race called “pardo”, 84,2% didn’t report alcohol consumption and just 12.5% reported tobacco use. Well differentiated LSCC was the most common histological grading (52,63%), especially in lower lip (57,8%). Status of Linfonodal involvement and metastasis were not informed in most cases (84,2%). Conclusion: In the North of Brazil, data of LSCC are scarce, probably due to the lack of studies and also due to the sub notification.
ACTIVIN A INHIBITS APOPTOSIS AND INDUCES ACQUISITION OF EMT PHENOTYPES IN NORMAL KERATINOCYTES Bufalino A1, Rodrigues PC1, Bastos DC1, Sammartino F1, Graner E1, Coletta RD1 - 1PIRACICABA
October 2012 DENTAL SCHOOL, UNIVERSITY OF CAMPINAS (UNICAMP) - ORAL DIAGNOSIS Objective: The purpose of this study was to investigate the effects of activin A, a member of TGF superfamily that has been shown to be overexpressed in various cancers, in the modulation of the key events associated with oral tumorigenesis, including proliferation, apoptosis and epithelial-mesenchymal transition (EMT). Study design: To assess the effects of activin A, the normal epithelial cell line HaCAT was cultured in medium containing 0-100 ng/ml of recombinant activin A. Results: Our results demonstrated that activin A promotes a dose-dependent decreased of apoptosis and death cell, with a significant inhibition on cell proliferation only at 100 ng/ml as revealed by cell cycle analysis and BrdU-labeling index. Additionally, activin A treatment stimulated significantly the expression of vimentin and N-cadherin, while reduced E-cadherin and -catenin expression. Conclusion: These findings demonstrate that activin A modulates apoptosis and acquisition of EMT phenotypes, contributing to oral tumorigenesis.
ONCOCYTIC METAPLASIA IN ORAL MYOEPITHELIOMA: REPORT OF A CASE Correia AL1, Gueiros Lam1, Leão JC1, Castro JFL1, León JE2, Almeida OP3, Perez DEC1 1 UNIVERSIDADE FEDERAL DE PERNAMBUCO - PROGRAMA DE PÓS GRADUAÇÃO EM ODONTOLOGIA, 2 UNIVERSIDADE DE SÃO PAULO - FACULDADE DE ODONTOLOGIA DE RIBEIRÃO PRETO, 3UNICAMP FACULDADE DE ODONTOLOGIA DE PIRACICABA The occurrence of oncocytic metaplasia in salivary gland tumors is a relatively common finding. However, depending on the extension, these areas may lead to misdiagnosis. The aim of this report is to describe a case of myoepithelioma with oncocytic metaplasia. A 40-year-old female presented a painless swelling in the hard palate with 4 years of evolution. Intraoral examination revealed a nodular lesion, with firm consistency and covered by normal color oral mucosa. A salivary gland tumor was the main hypothesis of diagnosis. Microscopically, the lesion was composed by epithelioid cells, which were arranged in nests and blocks. Several cells presented a granular, eosinophilic and enlargement cytoplasm, similar to oncocytic cells. The tumor cells were positive for AE1/AE3, cytokeratin 8 and p63. The diagnosis of myoepithelioma with oncocytic metaplasia was established. The patient was submitted to surgical excision of the lesion. Currently, the patient is under periodical follow up.
LIPOSARCOMA OF THE TONGUE: CASE REPORT Torrezani A1, Lima AN2, Esteves CMD3, Martins FM3, Nunes FD3, Milani B. de A2, Jorge WA2 - 1UNIVERSITY OF SÃO PAULO - STOMATOLOGY, 2MUNICIPAL HOSPITAL OF CAMPO LIMPO - MAXILLOFACIAL SURGERY, 3UNIVERSITY OF SÃO PAULO - ORAL PATHOLOGY Liposarcoma is a rare mesenchymal malignancy and affects more men, between the 4th and 6th decade of life. A 21-years-old male with a 4 year duration, asymptomatic swelling, on the dorsum of the tongue present himself for diagnosis. The clinical examination showed an approximately 4 cm nodule in diameter with ulcerated surface, erythroplasia, telangiectasia and a firm consistence. An incisional biopsy was made and the histopathological findings revealed a proliferation of round cells with hy-