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Acute phase reaction after liver transplantation: Cytokines and their products
238
237 SULFONIUM
SlGNIFfCANCE
IONS IN HEPATtC ENCEPHALOPATHY.
A. Tanoenan. S. Muller. J. RO(h&izen’. Dtv. ol Gastroi”teslinal and Liver Diseases, University Hospital. Nijmegen, The Netherlands and ‘Dept. of Animal Medicine, Utrecht, The Netherlands.
XC. #%
OF ANTi-HCV
LN ACUTE
HEPATtTfS
C
Tassopoulos. A. Hat&ii. I. Delladetsima. H. \‘assilowed~~tI \I houtelou. C Ka uroube,s,s. Dept. “t .\Ied,c,pe. S ii;“cml Hosp,,al “Uep,. of Hwene snd Ep,demiolo3&
&hens University Medic;1 School. A,hcd%reecc.
Sultonium ions are possi sulfur ions with Ihe sulfur bearing three substiiwnts. The best known suifonfwn fan fs S-adenosyknethionine (SAM). SAM has a labile msthyl group and acts as a methyl group donor in many transmethylarton reactions. SAM fs found to be towered in human cirrhosis. About 35 years ago. CMlenger et at. (Sk3chem.J. 56. 1954. iit refxxted amay sulfonlum ions in dog’s urine: CH3~s._X an.3 w Yet /S.-X CH,CH,CHf
““k”Lwn,
C”S%%%
sulfonium ions, when treated with alkali, were corwetled imo their volatile sultii methyl pfopyl sulftt and methyl butyl suttii. meseions~haveakMlemethylgrwpandmayalsoservea?i methyl donors. The aim of this audy was to develop reliable methods to measure the sutfonium iof0s in urfrm and serum of the dog and to investigate the relation hstwen the concentration of these ions and hepatic enoephalopathy (HE). Resuns: We davelopod a simple and vely sonshtte GC-MS method to measure the dog‘s sulfonium ions, after theic conversion into the respeot’nre sunides, using our advanced technique of he&space sampling and preconcentration onto Tenax trap tubes. Besides in urine. we were able to delect these ices also in serum of the dog. We found four sulfonium ions wkh struuc(ure:R,=methyl and R,=n-pfopyl. iso-butyl, n.butyl, or n-pentyl; X yet unknown. The total concentration d these sulfonium ions in the urine d 18 normal dogs varied from 15 to IW fimoVI. The concernration in serum was about 100 tknes smaller. In 13 out Or 16 dogs with HE, these suKonium fons were CMnplecely absent, while onty 3 dogs gave normal values. Thts dflerence between normal do&s and doas with HE was highly Sign’Mant. The relation between the absence of the dog’s sutkmium ions and the presence of HE has to be further evaluated.
These
239 HEPATITIS
C VlRUS
INFECtTON
IN DRUG ABUSERS
XC. Tarso~oulos. A. Hatzakis~. 51. Koutelou l-l. Vassilovoulou.\ted,cme. \\ ester” .+I,,&, tie”;ral HoytaL ‘0 d &%ie”e and Epidemiology. &hens Univenhy Me IWI Schzi &he%. Greece.
” “epl.01
To determine the prexilence and the im act of HCV infection in the course of xure hepatitis. 317 parentera Pdrug abusers lPD+.i with acute hepatitis [H;\:-i. HB:lSl, >.-\NBH 1. acute hepatitis superimosed on throne HBs,\e carrinae 43 tCH.BtI were studied. Four Plundred and thirtv one s&urn S&I les were t&ted for anti-HCV bv Ortho’s enzyme biununoassav Anu-%CV was detected in 142 lH.S%I of the 31T datienrs ,p,rl. In. articulsr. the re~alence of HCV infection was found to be 405 in h. 40.3% in 8B 54.55 in N&vBH and U.25 in CH-B. PD.4 Of the 4Y anti-HCV PDA with NANBH. 13 jeropositive early. on admission da!s after the onset ofsymptoms~. Anicteric disease was more frequent in these 13 Is than I” the remainine 35 ts t3S.55 M 17.14): this was also true eor the ore~alence of nnii-H Bc 161.5‘5 \5 20%. wO.051. These casesa?pe,ai to re resent acute exacerbations of chronic hepstitis C. Fulm”u”t aniP subtuhnmanr hepatitis were ohsewed onlv m 1 13.9%) of the 1Sl HB ts. The re~alence of HBV. t!CV and HDV serolo%,ad .P r, of ,he 1Sl HB p,s IS shown ,n rhe follow,n$ ~bi~~r, m p6_ ,b9
t +) (<7
t35 I were .
~erologlcol,“Kxcen
riral hepalmr
*t
i-“lml”anl
so.
ne,la,ms
.LO.
co
h Is clenr cul lhat Huk ilnd HCV coewrence UI amaated wh a sis_ ni$:uul~ higher risk of fulnd”a”t course p for trend = O.ooOl 1. rhrse data hhow Ihat: la) HCV has a remarkable preulence in Greek PQ.4. one fo@ of acute hepatitis C casesrepresents acute esncerbauom of chrome daense and IC) HCVcytotosicit~ may plan n” u” urtnnt role in the pathogeneais of Iulminnnt hcpotniiduc to HBV an8 HD\’ coiniections.
I
ib1
,c,*4,nl,,e* .\o. tartv LOSS
Posr,ble Risk Factor
Rood,rantus,onsj0 I\:DA 33 Needle-sick Sporadic 2; -rutal
‘ tlron,c
so. t3rty
Loss
NO.
S5
1;
7
3
;i(-;
’
100 1
3
119
s
29 3
S
IS
S
9
karlv aooearance of an,,-, .c \ war observed ,n 1, , l>.l’ r, Otthe DtS &die&b ti3%) of these pe developed chronic disease and thti might re resent acute exacerbations of chronic hepautb 5. &s
of
ano.HC e was observed UI 17 clO.lci~ of the qs ,cs,ed. In per,,cular anti-HCV serc ositivhv ws lransien, in S t-7.6? ! of the 29 Selflimited and 9 (1%.4%) ofthe chronic cases.In the laner group, loss of anti-HCV was characterized bv remission of chronic hepairir UL3 and aersisrence of the disease in ,hb remainine 6 m.5.
240 ACUTE PHASE REACTION AFTER LIVER TRANSPLANTATION: CYTDKINES AND THEIR PRODUCTS
. 11s. . W.Vocel. W.E.Aulitzkv. M.Herold, R. reiter. C Huber. T.A.Lu~~I Dept.Internal Medicins and Surgery, Univ. Hospital ,Innsbruck, Austria Dept.Derm.11, Vienna The aim of this study was to investigate serum levels of tumor necrosis factor alpha (TNF-a), IL-S, neopterin and C-reactiveprotein (CRP) in patients after orthotopic liver transplantation and to relate findings to clinical complications. Serum samples were collected from 16 patients every alternate day from transplantation until discharge. Concentrations of TNF-a and neopterin were performed using commercially available RIA's. CRP wa* measured with a nephelometric method and IL-6 using a sensitive bioassay. In six out of eight patients with acute cellular rejections, elevated levels of TNF-a and neopterin were detected. IL-6 and CRP concentrations were only markedly elevated in three out. of eight rejection cases. Bacterial infections- showed a different pattern with primarily increased concantrations of IL-6 and CRP. The highest absolute levels of TNF-a and IL-6 were observed in a patient with severe CMV disease. Evaluation of TNF-a. IL-S. CRP and neopterin may be useful in distinguishing typical complications following liver TX.
237 SULFONIUM
SlGNIFfCANCE
IONS IN HEPATtC ENCEPHALOPATHY.
A. Tanoenan. S. Muller. J. RO(h&izen’. Dtv. ol Gastroi”teslinal and Liver Diseases, University Hospital. Nijmegen, The Netherlands and ‘Dept. of Animal Medicine, Utrecht, The Netherlands.
XC. #%
OF ANTi-HCV
LN ACUTE
HEPATtTfS
C
Tassopoulos. A. Hat&ii. I. Delladetsima. H. \‘assilowed~~tI \I houtelou. C Ka uroube,s,s. Dept. “t .\Ied,c,pe. S ii;“cml Hosp,,al “Uep,. of Hwene snd Ep,demiolo3&
&hens University Medic;1 School. A,hcd%reecc.
Sultonium ions are possi sulfur ions with Ihe sulfur bearing three substiiwnts. The best known suifonfwn fan fs S-adenosyknethionine (SAM). SAM has a labile msthyl group and acts as a methyl group donor in many transmethylarton reactions. SAM fs found to be towered in human cirrhosis. About 35 years ago. CMlenger et at. (Sk3chem.J. 56. 1954. iit refxxted amay sulfonlum ions in dog’s urine: CH3~s._X an.3 w Yet /S.-X CH,CH,CHf
““k”Lwn,
C”S%%%
sulfonium ions, when treated with alkali, were corwetled imo their volatile sultii methyl pfopyl sulftt and methyl butyl suttii. meseions~haveakMlemethylgrwpandmayalsoservea?i methyl donors. The aim of this audy was to develop reliable methods to measure the sutfonium iof0s in urfrm and serum of the dog and to investigate the relation hstwen the concentration of these ions and hepatic enoephalopathy (HE). Resuns: We davelopod a simple and vely sonshtte GC-MS method to measure the dog‘s sulfonium ions, after theic conversion into the respeot’nre sunides, using our advanced technique of he&space sampling and preconcentration onto Tenax trap tubes. Besides in urine. we were able to delect these ices also in serum of the dog. We found four sulfonium ions wkh struuc(ure:R,=methyl and R,=n-pfopyl. iso-butyl, n.butyl, or n-pentyl; X yet unknown. The total concentration d these sulfonium ions in the urine d 18 normal dogs varied from 15 to IW fimoVI. The concernration in serum was about 100 tknes smaller. In 13 out Or 16 dogs with HE, these suKonium fons were CMnplecely absent, while onty 3 dogs gave normal values. Thts dflerence between normal do&s and doas with HE was highly Sign’Mant. The relation between the absence of the dog’s sutkmium ions and the presence of HE has to be further evaluated.
These
239 HEPATITIS
C VlRUS
INFECtTON
IN DRUG ABUSERS
XC. Tarso~oulos. A. Hatzakis~. 51. Koutelou l-l. Vassilovoulou.\ted,cme. \\ ester” .+I,,&, tie”;ral HoytaL ‘0 d &%ie”e and Epidemiology. &hens Univenhy Me IWI Schzi &he%. Greece.
” “epl.01
To determine the prexilence and the im act of HCV infection in the course of xure hepatitis. 317 parentera Pdrug abusers lPD+.i with acute hepatitis [H;\:-i. HB:lSl, >.-\NBH 1. acute hepatitis superimosed on throne HBs,\e carrinae 43 tCH.BtI were studied. Four Plundred and thirtv one s&urn S&I les were t&ted for anti-HCV bv Ortho’s enzyme biununoassav Anu-%CV was detected in 142 lH.S%I of the 31T datienrs ,p,rl. In. articulsr. the re~alence of HCV infection was found to be 405 in h. 40.3% in 8B 54.55 in N&vBH and U.25 in CH-B. PD.4 Of the 4Y anti-HCV PDA with NANBH. 13 jeropositive early. on admission da!s after the onset ofsymptoms~. Anicteric disease was more frequent in these 13 Is than I” the remainine 35 ts t3S.55 M 17.14): this was also true eor the ore~alence of nnii-H Bc 161.5‘5 \5 20%. wO.051. These casesa?pe,ai to re resent acute exacerbations of chronic hepstitis C. Fulm”u”t aniP subtuhnmanr hepatitis were ohsewed onlv m 1 13.9%) of the 1Sl HB ts. The re~alence of HBV. t!CV and HDV serolo%,ad .P r, of ,he 1Sl HB p,s IS shown ,n rhe follow,n$ ~bi~~r, m p6_ ,b9
t +) (<7
t35 I were .
~erologlcol,“Kxcen
riral hepalmr
*t
i-“lml”anl
so.
ne,la,ms
.LO.
co
h Is clenr cul lhat Huk ilnd HCV coewrence UI amaated wh a sis_ ni$:uul~ higher risk of fulnd”a”t course p for trend = O.ooOl 1. rhrse data hhow Ihat: la) HCV has a remarkable preulence in Greek PQ.4. one fo@ of acute hepatitis C casesrepresents acute esncerbauom of chrome daense and IC) HCVcytotosicit~ may plan n” u” urtnnt role in the pathogeneais of Iulminnnt hcpotniiduc to HBV an8 HD\’ coiniections.
I
ib1
,c,*4,nl,,e* .\o. tartv LOSS
Posr,ble Risk Factor
Rood,rantus,onsj0 I\:DA 33 Needle-sick Sporadic 2; -rutal
‘ tlron,c
so. t3rty
Loss
NO.
S5
1;
7
3
;i(-;
’
100 1
3
119
s
29 3
S
IS
S
9
karlv aooearance of an,,-, .c \ war observed ,n 1, , l>.l’ r, Otthe DtS &die&b ti3%) of these pe developed chronic disease and thti might re resent acute exacerbations of chronic hepautb 5. &s
of
ano.HC e was observed UI 17 clO.lci~ of the qs ,cs,ed. In per,,cular anti-HCV serc ositivhv ws lransien, in S t-7.6? ! of the 29 Selflimited and 9 (1%.4%) ofthe chronic cases.In the laner group, loss of anti-HCV was characterized bv remission of chronic hepairir UL3 and aersisrence of the disease in ,hb remainine 6 m.5.
240 ACUTE PHASE REACTION AFTER LIVER TRANSPLANTATION: CYTDKINES AND THEIR PRODUCTS
. 11s. . W.Vocel. W.E.Aulitzkv. M.Herold, R. reiter. C Huber. T.A.Lu~~I Dept.Internal Medicins and Surgery, Univ. Hospital ,Innsbruck, Austria Dept.Derm.11, Vienna The aim of this study was to investigate serum levels of tumor necrosis factor alpha (TNF-a), IL-S, neopterin and C-reactiveprotein (CRP) in patients after orthotopic liver transplantation and to relate findings to clinical complications. Serum samples were collected from 16 patients every alternate day from transplantation until discharge. Concentrations of TNF-a and neopterin were performed using commercially available RIA's. CRP wa* measured with a nephelometric method and IL-6 using a sensitive bioassay. In six out of eight patients with acute cellular rejections, elevated levels of TNF-a and neopterin were detected. IL-6 and CRP concentrations were only markedly elevated in three out. of eight rejection cases. Bacterial infections- showed a different pattern with primarily increased concantrations of IL-6 and CRP. The highest absolute levels of TNF-a and IL-6 were observed in a patient with severe CMV disease. Evaluation of TNF-a. IL-S. CRP and neopterin may be useful in distinguishing typical complications following liver TX.