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Administration of ethanol to pregnant rats affects the fluidity of brain membranes of newborn PUPS
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61
THE SPATIAL DENSITY PATTERNS OF THE OPEN ION CHANNELS AS DISSIPATIVE STRUCTURES.
L SAVTCHENKO, S KOROGOD - Lab. of Biophysics and Bioelectronics, Dniepropetrovsk State Bniv., Ukraine. Electrical spatial patterns were investigated in various biological unicellular systems (particularly neurons) by means of a mathematical model represented by the non-linear differential equations describing the distribution of the open ion channels, transmembrane potentials and ion concentrations. We associated spatial profiles of the transmembranc potential with concentration profiles of the open ion channels. The following condition of the channel pattern formation was found: dependence of lateral diffusivity of channels in the membrane on their conformation state (open or closed). It was suggested that the lateral membrane pattern of the opened ion channels belongs to a group of the self-organized dynamic structures maintained due to the energy of dephosphorylation of ATP molecules. 63
PROTECTION FROM OXIDATIVE DESTRUCTION OF RAT BRAIN SY?WPTOSOMAL MONOAMINOOXIDASE BY LIPID AND WATER SOLUBLE ANTIOXIDANTS V.A.Tyurin, A.D.Antipov,V.P Chelomin, N.F.Avrova, V.E.Kagan. Inst. of Evol. Physiol. & Bioohem., Ao. Soi. of Russia, St.Peterburg. The transition metal6 (Fe*+or Cu2' in the presenoe or absenoe of nou6 reduoing agents) were fou$Og?o inhibit monoaminooxidase (MAO) of rat brain synaptosomes and to oame its transformation.They were found to oxidize the SH-group of membrane proteins.to induoe LPO. inorease miorovisooaity and deorease hydrophobioity of synaptosomal membranes. The preinoubation of synaptosomes with U-tooopherol markedly deoreased the inaotivation of MAO and the ohange of its substrate speoifioity, prevented the oxidative destruotion of protein and fatty aoid lipids, the ohange of transbilayer asymmetry, the effeot of oarnosine-being-less-pronounoed. If both liDid and water soluble antioxidants w&e used together their effeot waB found to be synergistio.
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62 ADMINISTRATION OF ETHANOL TO PRBGNNT RATS AFFECTS T‘HE FLUIDITY OF BRAIN MFlblBRAElEs OF NEWBORN PUPS 0. Arienti*, 0. C. Di RenxoS, E. F. Battietoni., P. Wastrofini* Coraxxi*
Carlini*, and L.
lIstituto di Biochimica, Via de1 Giochetto, 06100 PERUMA (Italy), 6 Istituto di Qinecologia, Dhiversith di Perugia, 06100 PERUGIA (Italy) and "U.S.L. di Assisi, ASSISI (PO, Italy) The biochemical bases of the "Fetal Alcohol Syndrome" are unknown. We test the fluidity of brain microsomss by the determining fluorescence ahisotropy of diphenylhexatriene (DPH) and of trimethylaminodipehnylhexatriehe (TWA-DPH). We gave ethanol to pregnant dams by using the diet by De Carli and Lieber which contained 18% of energy as ethanol (treated); the alcohol was replaced 8ucrose in controls. We measured by fluorescehce ahisotropy 10-C to (r) from 54-C with incr-ts of 1-C. The relationship of r VP. twrature was linear ahd the slo~a of the line was steeper in treated pups (both probes). TO ascertain whether the observed effecta were due to ethanol itself or to ita metabolism, w* performed in vitro experiments and noticed that only the r for DPH was affected (but only if the concentration of ethanol was 20.3 M). We conclude that the effects of ethanol in viva are mainly due to its metabolism. 64
61
THE SPATIAL DENSITY PATTERNS OF THE OPEN ION CHANNELS AS DISSIPATIVE STRUCTURES.
L SAVTCHENKO, S KOROGOD - Lab. of Biophysics and Bioelectronics, Dniepropetrovsk State Bniv., Ukraine. Electrical spatial patterns were investigated in various biological unicellular systems (particularly neurons) by means of a mathematical model represented by the non-linear differential equations describing the distribution of the open ion channels, transmembrane potentials and ion concentrations. We associated spatial profiles of the transmembranc potential with concentration profiles of the open ion channels. The following condition of the channel pattern formation was found: dependence of lateral diffusivity of channels in the membrane on their conformation state (open or closed). It was suggested that the lateral membrane pattern of the opened ion channels belongs to a group of the self-organized dynamic structures maintained due to the energy of dephosphorylation of ATP molecules. 63
PROTECTION FROM OXIDATIVE DESTRUCTION OF RAT BRAIN SY?WPTOSOMAL MONOAMINOOXIDASE BY LIPID AND WATER SOLUBLE ANTIOXIDANTS V.A.Tyurin, A.D.Antipov,V.P Chelomin, N.F.Avrova, V.E.Kagan. Inst. of Evol. Physiol. & Bioohem., Ao. Soi. of Russia, St.Peterburg. The transition metal6 (Fe*+or Cu2' in the presenoe or absenoe of nou6 reduoing agents) were fou$Og?o inhibit monoaminooxidase (MAO) of rat brain synaptosomes and to oame its transformation.They were found to oxidize the SH-group of membrane proteins.to induoe LPO. inorease miorovisooaity and deorease hydrophobioity of synaptosomal membranes. The preinoubation of synaptosomes with U-tooopherol markedly deoreased the inaotivation of MAO and the ohange of its substrate speoifioity, prevented the oxidative destruotion of protein and fatty aoid lipids, the ohange of transbilayer asymmetry, the effeot of oarnosine-being-less-pronounoed. If both liDid and water soluble antioxidants w&e used together their effeot waB found to be synergistio.
B16
62 ADMINISTRATION OF ETHANOL TO PRBGNNT RATS AFFECTS T‘HE FLUIDITY OF BRAIN MFlblBRAElEs OF NEWBORN PUPS 0. Arienti*, 0. C. Di RenxoS, E. F. Battietoni., P. Wastrofini* Coraxxi*
Carlini*, and L.
lIstituto di Biochimica, Via de1 Giochetto, 06100 PERUMA (Italy), 6 Istituto di Qinecologia, Dhiversith di Perugia, 06100 PERUGIA (Italy) and "U.S.L. di Assisi, ASSISI (PO, Italy) The biochemical bases of the "Fetal Alcohol Syndrome" are unknown. We test the fluidity of brain microsomss by the determining fluorescence ahisotropy of diphenylhexatriene (DPH) and of trimethylaminodipehnylhexatriehe (TWA-DPH). We gave ethanol to pregnant dams by using the diet by De Carli and Lieber which contained 18% of energy as ethanol (treated); the alcohol was replaced 8ucrose in controls. We measured by fluorescehce ahisotropy 10-C to (r) from 54-C with incr-ts of 1-C. The relationship of r VP. twrature was linear ahd the slo~a of the line was steeper in treated pups (both probes). TO ascertain whether the observed effecta were due to ethanol itself or to ita metabolism, w* performed in vitro experiments and noticed that only the r for DPH was affected (but only if the concentration of ethanol was 20.3 M). We conclude that the effects of ethanol in viva are mainly due to its metabolism. 64