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Aerobic training stimulates mitochondrial enzyme expression and initiates skeletal muscle fibre shifting in rat soleus muscle
Journal Abstracts
17
T1:O.015
T3:P.016
The prevalence and characteristics associated with excessive daytime sleepiness among Australian workers
Aerobic training stimulates mitochondrial enzyme expression and initiates skeletal muscle fibre shifting in rat soleus muscle
W.L. Ng 1,2,∗ , A. Peeters 1 , R. Freak-Poli 1
R. Farenia ∗ , R. Lesmana
1 Baker
IDI Heart and Diabetes Institute, Monash University, Melbourne, Australia 2 University of Indonesia, Indonesia Introduction: Excessive daytime sleepiness (EDS) is a growing health problem, especially in workplaces, where it has been associated with increased rate of morbidity and mortality and reduced quality of performance. Our primary aim is to estimate the prevalence of EDS and its associated factors in a general working population. Methods: The data of 707 eligible study participants were obtained from the Global Corporate Challenge® Evaluation Study, a prospective cohort study of voluntary participants in a workplace pedometer program. Daytime sleepiness was measured using the Epworth Sleepiness Scale (ESS) questionnaires. EDS was defined as ESS > 10. Associations between demographics; reasons for participation; dietary pattern; behavioural, psychosocial, anthropometric and biomedical factors with EDS were estimated using multivariate logistic model. Results: In this population of adult employees (60% male, mean age 40), prevalence of EDS was 16.0%. Characteristics associated with a higher risk of EDS were lower income (odds ratio (OR) 2.3 (95% CI 1.2—4.4) for lowest vs. highest income group), poorer diet (OR 4.6 (95% CI 2.2—9.3) for frequent take-away food, and OR 0.33 (95% CI 0.1—0.8%) for least vs. most frequent snacking), and poorer mental health (OR 0.9 (95% CI 0.8—0.9) for each unit increase in wellbeing). Conclusion: Our study has captured the scale of EDS and identified its associated characteristics in an Australian general working population. This may contribute to better identification and targeting of workers with EDS in potential future interventions to alleviate EDS. Keywords: Excessive daytime sleepiness; Epworth Sleepiness scale; Workers http://dx.doi.org/10.1016/j.orcp.2013.08.054
Department of Physiology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia Introduction: Exercise alters skeletal muscle function and structure. Exercise intensity could affects differently to skeletal muscle metabolism and its morphological structure. Myoglobin is one of marker which may be involved and altered in order to fulfil cell energy demand. However there is a little information about aerobic training to mitochondrial enzyme and histological changes. Method: Male Wistar rat, were trained with different exercise intensity (15, 20 and 25 m/min) for 30 min per day for 14 days. Rats were anaesthesia with ether, jugular vein was carefully exposed and blood sample was collected. Blood sample were proposed for measuring CPK level and myoglobin level. At day fourteen, after last exercise rat were sacrificed under ether anaesthesia, soleus muscle were dissected out for quantitative RT PCR. Soleus muscle was stained using ATPase and Hematoxylin Eosin staining. Result: This study observed consistent increase of myoglobin mRNA expression in both exercise group, and this gene expression attenuation also found in gene which has role in growth and metabolism of the muscle such as: MyoD, MHC I, citrate synthase and G6PD. There is a regeneration sign in soleus muscle from aerobic group and distropy sign in anaerobic group. This sign may a clue of increasing of myoglobin serum and mRNA expression at day 14. Conclusion: Taken together, different exercise intensity plays an important role in metabolic adaptive process and rearrangement of muscle histological structure. During this adaptation process, myoglobin and CPK can be used as a good indicator for stress exercise indicator. Keywords: Aerobic training; Mitochondrial enzyme expressions; Rat soleus muscle http://dx.doi.org/10.1016/j.orcp.2013.08.055
17
T1:O.015
T3:P.016
The prevalence and characteristics associated with excessive daytime sleepiness among Australian workers
Aerobic training stimulates mitochondrial enzyme expression and initiates skeletal muscle fibre shifting in rat soleus muscle
W.L. Ng 1,2,∗ , A. Peeters 1 , R. Freak-Poli 1
R. Farenia ∗ , R. Lesmana
1 Baker
IDI Heart and Diabetes Institute, Monash University, Melbourne, Australia 2 University of Indonesia, Indonesia Introduction: Excessive daytime sleepiness (EDS) is a growing health problem, especially in workplaces, where it has been associated with increased rate of morbidity and mortality and reduced quality of performance. Our primary aim is to estimate the prevalence of EDS and its associated factors in a general working population. Methods: The data of 707 eligible study participants were obtained from the Global Corporate Challenge® Evaluation Study, a prospective cohort study of voluntary participants in a workplace pedometer program. Daytime sleepiness was measured using the Epworth Sleepiness Scale (ESS) questionnaires. EDS was defined as ESS > 10. Associations between demographics; reasons for participation; dietary pattern; behavioural, psychosocial, anthropometric and biomedical factors with EDS were estimated using multivariate logistic model. Results: In this population of adult employees (60% male, mean age 40), prevalence of EDS was 16.0%. Characteristics associated with a higher risk of EDS were lower income (odds ratio (OR) 2.3 (95% CI 1.2—4.4) for lowest vs. highest income group), poorer diet (OR 4.6 (95% CI 2.2—9.3) for frequent take-away food, and OR 0.33 (95% CI 0.1—0.8%) for least vs. most frequent snacking), and poorer mental health (OR 0.9 (95% CI 0.8—0.9) for each unit increase in wellbeing). Conclusion: Our study has captured the scale of EDS and identified its associated characteristics in an Australian general working population. This may contribute to better identification and targeting of workers with EDS in potential future interventions to alleviate EDS. Keywords: Excessive daytime sleepiness; Epworth Sleepiness scale; Workers http://dx.doi.org/10.1016/j.orcp.2013.08.054
Department of Physiology, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia Introduction: Exercise alters skeletal muscle function and structure. Exercise intensity could affects differently to skeletal muscle metabolism and its morphological structure. Myoglobin is one of marker which may be involved and altered in order to fulfil cell energy demand. However there is a little information about aerobic training to mitochondrial enzyme and histological changes. Method: Male Wistar rat, were trained with different exercise intensity (15, 20 and 25 m/min) for 30 min per day for 14 days. Rats were anaesthesia with ether, jugular vein was carefully exposed and blood sample was collected. Blood sample were proposed for measuring CPK level and myoglobin level. At day fourteen, after last exercise rat were sacrificed under ether anaesthesia, soleus muscle were dissected out for quantitative RT PCR. Soleus muscle was stained using ATPase and Hematoxylin Eosin staining. Result: This study observed consistent increase of myoglobin mRNA expression in both exercise group, and this gene expression attenuation also found in gene which has role in growth and metabolism of the muscle such as: MyoD, MHC I, citrate synthase and G6PD. There is a regeneration sign in soleus muscle from aerobic group and distropy sign in anaerobic group. This sign may a clue of increasing of myoglobin serum and mRNA expression at day 14. Conclusion: Taken together, different exercise intensity plays an important role in metabolic adaptive process and rearrangement of muscle histological structure. During this adaptation process, myoglobin and CPK can be used as a good indicator for stress exercise indicator. Keywords: Aerobic training; Mitochondrial enzyme expressions; Rat soleus muscle http://dx.doi.org/10.1016/j.orcp.2013.08.055