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Affinity chromatography: a practical approach
540
BOOK REVIEWS
On the whole, the book is well-produced with many useful diagrams, graphs, tables, and chromatograms. The references cover the literature up to 1982. This book is highly recommended to graduate students, research workers, and anyone wishing to improve his or her skills in liquid chromatography. Nutley, NJ (U.S.A.)
PAUL KUCERA
Journal of Chromatography, 354 (1986) 540
Elsevier Science Publishers B.V., Amsterdam -
Printed in The Netherlands
CHROM. 18 402
Book Review
Afinity chromatography: a practical approach, edited by P. D. G. Dean, W. S. Johnson and F. A. Middle, IRL Press, Oxford, Washington, DC, 1985, XVI + 215 pp., price f 11.00, US% 20.00, ISBN 0-904147-71-l. This appears to be the first book in the field of chromatography to be written along the lines of “Organic Syntheses” or “Inorganic Syntheses”, in that it gives complete methods of synthesis for affinity chromatography supports and is also written by a multitude of authors, twenty-five to be exact. It is divided into eight main chapters. The first deals with matrix preparations and applications and makes interesting reading. Besides the usual matrices it also mentions Eupergit C and Kimal, both not familiar to the reviewer. Table 3 lists the commercial sources of supports. The coverage seems adequate, but unfortunately no addresses of the 8rms are given. Chapter 2 describes activation procedures, including even one for cellulose paper. In the next chapter on “Cross-linking agents for coupling matrices to spacers”, a useful table lists the bifunctional compounds. The various methods also draw attention to the possible hazards in the preparation methods. A wise precaution, as many biochemists nowadays are not experienced in preparative organic chemistry. Chapter 4 is entitled “Operational Methodologies”. The exact meaning of this compound term still escapes me, but the subsections may enlighten the reader, namely: Determination of ligand concentration, Ligand leakage, Ligand concentration effects, Effect of column dimensions, pH and temperature effects, Ionic strength, Desorption methods, Use of cylindrical baffles in large scale affinity columns, Design and use of high performance affinity-chromatography minicolumns. Ligands for immobilisation is the topic of Chapter 5 and covers the topic adequately, but of course not completely in 48 pages and 74 references. Chapters 6 and 7 survey quantitative and analytical aspects. The book concludes with a chapter on cell separation via cleavable mercurysulphur bonds. There is a subject but no author index. In addition to providing methods the book is also a welcome, if incomplete, reference work. It should be on the shelf of every chromatographer.
BOOK REVIEWS
On the whole, the book is well-produced with many useful diagrams, graphs, tables, and chromatograms. The references cover the literature up to 1982. This book is highly recommended to graduate students, research workers, and anyone wishing to improve his or her skills in liquid chromatography. Nutley, NJ (U.S.A.)
PAUL KUCERA
Journal of Chromatography, 354 (1986) 540
Elsevier Science Publishers B.V., Amsterdam -
Printed in The Netherlands
CHROM. 18 402
Book Review
Afinity chromatography: a practical approach, edited by P. D. G. Dean, W. S. Johnson and F. A. Middle, IRL Press, Oxford, Washington, DC, 1985, XVI + 215 pp., price f 11.00, US% 20.00, ISBN 0-904147-71-l. This appears to be the first book in the field of chromatography to be written along the lines of “Organic Syntheses” or “Inorganic Syntheses”, in that it gives complete methods of synthesis for affinity chromatography supports and is also written by a multitude of authors, twenty-five to be exact. It is divided into eight main chapters. The first deals with matrix preparations and applications and makes interesting reading. Besides the usual matrices it also mentions Eupergit C and Kimal, both not familiar to the reviewer. Table 3 lists the commercial sources of supports. The coverage seems adequate, but unfortunately no addresses of the 8rms are given. Chapter 2 describes activation procedures, including even one for cellulose paper. In the next chapter on “Cross-linking agents for coupling matrices to spacers”, a useful table lists the bifunctional compounds. The various methods also draw attention to the possible hazards in the preparation methods. A wise precaution, as many biochemists nowadays are not experienced in preparative organic chemistry. Chapter 4 is entitled “Operational Methodologies”. The exact meaning of this compound term still escapes me, but the subsections may enlighten the reader, namely: Determination of ligand concentration, Ligand leakage, Ligand concentration effects, Effect of column dimensions, pH and temperature effects, Ionic strength, Desorption methods, Use of cylindrical baffles in large scale affinity columns, Design and use of high performance affinity-chromatography minicolumns. Ligands for immobilisation is the topic of Chapter 5 and covers the topic adequately, but of course not completely in 48 pages and 74 references. Chapters 6 and 7 survey quantitative and analytical aspects. The book concludes with a chapter on cell separation via cleavable mercurysulphur bonds. There is a subject but no author index. In addition to providing methods the book is also a welcome, if incomplete, reference work. It should be on the shelf of every chromatographer.