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Affinity chromatography and biological recognition
ANALYTICAL BIOCHEMISTRY l&,305-306
(1985)
BOOK REVIEWS Receptor Biochemistry and Methodology, Vol. 1, Membranes, Detergents, and Receptor Solubilization; Vol. 2, Receptor Purification Procedures; Vol. 3, Molecular and Chemical Characterization of Membrane Recep tors. Edited by CRAIG VENTER AND LEONARD C. HARRISON. Alan R. Liss, Inc., New York, 1984. I, 225 pp.; II, 171 pp.: III, 293 pp. Over the past decade, there has been rapid growth in research into the structure and function of cell surface receptors. These studies have exploited most of the techniques of modern biochemistry, cell biology, and molecular biology. Consequently, it is ambitious to attempt to review this area in approximately 700 pages. The selection of topics is varied, emphasizing methodological issues. Certain chapters (e.g., “Solubilization of functional membrane-bound receptors,” “Affinity crosslinking of peptide hormones and their receptors,” and “Reconstitution of membrane receptors”) provide scholarly discussion of subjects specifically relevant to cell surface receptors. At the other extreme is a chapter entitled “Determination of membrane protein concentrations” which lists and discusses some of the standard
AfIinity Chromatography and Biological Recognition. Edited by IRWIN M. CHAIKEN, MEIR WILCHEK, AND INDU PARIKH, Academic Press, New York, 1983. 515 pp. $43.50. This volume presents the Proceedings of the Fifth International Symposium on Affinity Chromatography and Biological Recognition. The contents are divided into an introduction (part I) on general principles and considerations of molecular recognition (I. Chaiken and E. Katchalski-Katzir) followed by five separate parts. Each of these chapters contains a number of contributions which cover a wide spectrum of subjects that should prove of value to investigators interested in affinity chromatography and biological recognition. Examples of molecular interaction in biorecognition are presented in part II. Subjects such as mechanistic aspects of the action of membrane receptors (P. Cuatrecasas), cellular recognition (R. L. Schnaar), calmodulin as a cytosolic regulator (C. B. Klee), and hemoglobin subunit interaction (G. K. Ackers), to mention a few, are discussed. 305
laboratory methods available for measuring protein concentration. While some chapters are of general interest (e.g., “Prospects for the isolation of genes for receptors and other proteins of pharmacological and neurobiological interest”), others Seems excessively specialized (e.g., “Binding and equilibrium studies using an air-driven ultracentrifuge,” or “Affinity phase partitioning”). As with most multiauthor works, the chapters are uneven in quality and the three-volume work lacks clear focus. Nevertheless, there is much here that will interest investigators in the receptor field. Most of the chapters have references as recent as 1983. Because of the rapidity of progress, the absence of 1984 references makes a few chapters seem dated (e.g., “Prospects for the isolation of genes for receptors and other proteins of pharmacological and neurobiological interest”). However, for the most part, these volumes provide a relatively up-to-date, methodologically oriented review of the receptor field. Most medical libraries and many laboratories will want to purchase these volumes as reference works. SIMEON TAYLOR
A more quantitative approach to the understanding of affinity methods that involve biomolecular interactions is presented in part III. Contributions by the laboratories of Chaiken, De Lisi, Inman, and Winzor are mentioned. These as well as other papers in this chapter offer valuable information for determining binding constants and kinetic parameters. Various types of affinity methods, their design, and development are provided in part IV. The contributions are too numerous to mention all, but a few examples sufficeto illustrate the contents of this section. Preparation of activated polysaccharide resins by the use of novel cyanogen bromide reactions (M. Wilchek), new atlinity techniques (K. Mosbach), hydrophobic chromatography (S. Shaltiel), and the extension of the principle of affinity chromatography to high-performance liquid chromatography (S. Ohlson, M. Glad, P. 0. Iarsson) are representative examples. Affinity methods that use antibodies are presented in part V. Antibodies as diagnostic and therapeutic tools make up the contents of the first four papers (E. Haber, M. Wilchek, J. N. Weinstein et nl., and T. M. Shinnik 0003-2697185 $3.00 Copyright 0 1985 by Academic Press. Inc. All rights of reproduction in any form reserved.
306
BOOK REVIEWS
et al.). This is followed by several papers on the application of immunosorbent techniques to describe the application of antibodies to chromatography. The volume concludes with examples of applications of affinity chromatography (part VI) to achieve specific separations of biologically important macromolecules. This book is a notable exception to similar “proceedings reports” which too often are compilations of loosely related subjects and are of little value to readers outside the field. In this instance there is valuable general infor-
mation for investigators interested in affinity chromatography or in studies of the phenomenon of biological recognition. Credit is due to the editors, as well as to the organizing committee, for the selection and presentation of a timely subject by investigators actively involved in the field. It is gratifying to note the increased symbiotic relationship that continues to develop between affinity chromatography and biological recognition. OTHMAR
GABRIEL
(1985)
BOOK REVIEWS Receptor Biochemistry and Methodology, Vol. 1, Membranes, Detergents, and Receptor Solubilization; Vol. 2, Receptor Purification Procedures; Vol. 3, Molecular and Chemical Characterization of Membrane Recep tors. Edited by CRAIG VENTER AND LEONARD C. HARRISON. Alan R. Liss, Inc., New York, 1984. I, 225 pp.; II, 171 pp.: III, 293 pp. Over the past decade, there has been rapid growth in research into the structure and function of cell surface receptors. These studies have exploited most of the techniques of modern biochemistry, cell biology, and molecular biology. Consequently, it is ambitious to attempt to review this area in approximately 700 pages. The selection of topics is varied, emphasizing methodological issues. Certain chapters (e.g., “Solubilization of functional membrane-bound receptors,” “Affinity crosslinking of peptide hormones and their receptors,” and “Reconstitution of membrane receptors”) provide scholarly discussion of subjects specifically relevant to cell surface receptors. At the other extreme is a chapter entitled “Determination of membrane protein concentrations” which lists and discusses some of the standard
AfIinity Chromatography and Biological Recognition. Edited by IRWIN M. CHAIKEN, MEIR WILCHEK, AND INDU PARIKH, Academic Press, New York, 1983. 515 pp. $43.50. This volume presents the Proceedings of the Fifth International Symposium on Affinity Chromatography and Biological Recognition. The contents are divided into an introduction (part I) on general principles and considerations of molecular recognition (I. Chaiken and E. Katchalski-Katzir) followed by five separate parts. Each of these chapters contains a number of contributions which cover a wide spectrum of subjects that should prove of value to investigators interested in affinity chromatography and biological recognition. Examples of molecular interaction in biorecognition are presented in part II. Subjects such as mechanistic aspects of the action of membrane receptors (P. Cuatrecasas), cellular recognition (R. L. Schnaar), calmodulin as a cytosolic regulator (C. B. Klee), and hemoglobin subunit interaction (G. K. Ackers), to mention a few, are discussed. 305
laboratory methods available for measuring protein concentration. While some chapters are of general interest (e.g., “Prospects for the isolation of genes for receptors and other proteins of pharmacological and neurobiological interest”), others Seems excessively specialized (e.g., “Binding and equilibrium studies using an air-driven ultracentrifuge,” or “Affinity phase partitioning”). As with most multiauthor works, the chapters are uneven in quality and the three-volume work lacks clear focus. Nevertheless, there is much here that will interest investigators in the receptor field. Most of the chapters have references as recent as 1983. Because of the rapidity of progress, the absence of 1984 references makes a few chapters seem dated (e.g., “Prospects for the isolation of genes for receptors and other proteins of pharmacological and neurobiological interest”). However, for the most part, these volumes provide a relatively up-to-date, methodologically oriented review of the receptor field. Most medical libraries and many laboratories will want to purchase these volumes as reference works. SIMEON TAYLOR
A more quantitative approach to the understanding of affinity methods that involve biomolecular interactions is presented in part III. Contributions by the laboratories of Chaiken, De Lisi, Inman, and Winzor are mentioned. These as well as other papers in this chapter offer valuable information for determining binding constants and kinetic parameters. Various types of affinity methods, their design, and development are provided in part IV. The contributions are too numerous to mention all, but a few examples sufficeto illustrate the contents of this section. Preparation of activated polysaccharide resins by the use of novel cyanogen bromide reactions (M. Wilchek), new atlinity techniques (K. Mosbach), hydrophobic chromatography (S. Shaltiel), and the extension of the principle of affinity chromatography to high-performance liquid chromatography (S. Ohlson, M. Glad, P. 0. Iarsson) are representative examples. Affinity methods that use antibodies are presented in part V. Antibodies as diagnostic and therapeutic tools make up the contents of the first four papers (E. Haber, M. Wilchek, J. N. Weinstein et nl., and T. M. Shinnik 0003-2697185 $3.00 Copyright 0 1985 by Academic Press. Inc. All rights of reproduction in any form reserved.
306
BOOK REVIEWS
et al.). This is followed by several papers on the application of immunosorbent techniques to describe the application of antibodies to chromatography. The volume concludes with examples of applications of affinity chromatography (part VI) to achieve specific separations of biologically important macromolecules. This book is a notable exception to similar “proceedings reports” which too often are compilations of loosely related subjects and are of little value to readers outside the field. In this instance there is valuable general infor-
mation for investigators interested in affinity chromatography or in studies of the phenomenon of biological recognition. Credit is due to the editors, as well as to the organizing committee, for the selection and presentation of a timely subject by investigators actively involved in the field. It is gratifying to note the increased symbiotic relationship that continues to develop between affinity chromatography and biological recognition. OTHMAR
GABRIEL