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AHR-mediated oxidative stress contributes to the cardiac developmental toxicity of trichloroethylene in zebrafish embryos
Journal Pre-proof AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos Hongmei Jin, Cheng Ji, Fei Ren, Stanley Aniagu, Jian Tong, Yan Jiang, Tao Chen
PII:
S0304-3894(19)31475-X
DOI:
https://doi.org/10.1016/j.jhazmat.2019.121521
Reference:
HAZMAT 121521
To appear in:
Journal of Hazardous Materials
Received Date:
21 May 2019
Revised Date:
14 October 2019
Accepted Date:
21 October 2019
Please cite this article as: Jin H, Ji C, Ren F, Aniagu S, Tong J, Jiang Y, Chen T, AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos, Journal of Hazardous Materials (2019), doi: https://doi.org/10.1016/j.jhazmat.2019.121521
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Published by Elsevier.
AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos
Hongmei Jin1,#, Cheng Ji1,#, Fei Ren1,2, Stanley Aniagu3, Jian Tong1,2 , Yan Jiang1, *, and Tao Chen1,2,*
Medical College of Soochow University, Suzhou, P.R. China.
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Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric
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Diseases, Soochow University, Suzhou, China.
Toxicology, Risk Assessment and Research Division, Texas Commission on
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Environmental Quality, 12015 Park 35 Cir, Austin, TX, USA
Corresponding authors:
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*
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# These two authors contributed equally to the paper.
Dr. Yan Jiang, Medical College of Soochow University, Soochow University, 199
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Ren-Ai Road, Suzhou China. 215123; Email: [email protected]
Dr. Tao Chen, Medical College of Soochow University, Soochow University, 199 Ren-Ai Road, Suzhou China. 215123; Email: [email protected]
Graphical abstract
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Highlights:
AHR inhibition significantly counteracted the TCE-induced heart defects.
TCE upregulated the expression levels of cyp1b1 but not cyp1a1.
TCE-induced ROS production led to heart defects in zebrafish embryos.
AHR mediated the TCE-induced oxidative stress.
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Abstract
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Trichloroethylene (TCE), a widely used chlorinated solvent, is a common environmental pollutant. Current evidence shows that TCE could induce heart defects during embryonic development, but the underlining mechanism(s) remain unclear. Since activation of the aryl hydrocarbon receptor (AHR) could induce oxidative stress, we hypothesized that AHR-mediated oxidative stress may play a role in the cardiac developmental toxicity of TCE. In this study, we found that the 2
reactive oxygen species (ROS) scavenger, N-Acetyl-L-cysteine (NAC), and AHR inhibitors, CH223191 (CH) and StemRegenin 1, significantly counteracted the TCE-induced heart malformations in zebrafish embryos. Moreover, both CH and NAC suppressed TCE-induced ROS and 8-OHdG (8-hydroxy-2' -deoxyguanosine). TCE did not affect ahr2 and cyp1a expression, but increased cyp1b1 expression, which was restored by CH supplementation. CH also attenuated the TCE-induced
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mRNA expression changes of Nrf2 signalling genes (nrf2b, gstp2, sod2, ho1, nqo1) and cardiac differentiation genes (gata4, hand2, c-fos, sox9b). In addition, the TCE
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enhanced SOD activity was attenuated by CH. Morpholino knockdown confirmed that AHR mediated the TCE-induced ROS and 8-OHdG generation in the heart of
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zebrafish embryos. In conclusion, our results suggest that AHR mediates
zebrafish embryos.
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TCE-induced oxidative stress, leading to DNA damage and heart malformations in
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Keyword：Trichloroethylene; Aryl Hydrocarbon Receptor; Reactive Oxygen Species;
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Heart development; Zebrafish embryos
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1. Introduction
Trichloroethylene (TCE) is a widely used volatile organic compound (VOC) that has been frequently detected in air, groundwater and soil (EPA, 2011). It is ranked 16th in the priority list of hazardous substances in the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) (ATSDR, 2007). 3
Epidemiological studies have linked maternal TCE exposure to spontaneous abortions, low birth weight, and congenital heart defects (CHDs) (Makris et al., 2016). Reports from animal experiments also showed various cardiac defects induced by TCE in avians, rats and zebrafish (Caldwell et al., 2008; Drake et al., 2006; Rufer et al., 2010; Wirbisky et al., 2016). In addition, we previously demonstrated that TCE inhibited cardiac differentiation in human stem cells (Jiang et al., 2016). After absorption, TCE
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is metabolized through two main pathways: Cytochrome P450 (CYP)-dependent
oxidation and glutathione (GSH) conjugation (Lash et al., 2000). It is worth noting
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that trichloroacetic acid (TCA), the main oxidative metabolite of TCE in human, was reported to induce cardiac defects in rats (Johnson et al., 1998b).
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Previously, there were several studies indicating that TCE treatment could induce
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oxidative stress in human cell lines as well as in animal models such as mice and rats (Blossom et al., 2008; Blossom et al., 2017; Toraason et al., 1999). Oxidative stress
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has been suggested to play a crucial role in the pathophysiology of CHDs (Asoglu et al., 2018). In addition, reactive oxygen species (ROS) has also been reported to be
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involved in cardiomyocyte differentiation of pluripotent stem cells (Wei and Cong,
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2018). The adaptive response to oxidative stress is mainly regulated by nuclear factor erythroid 2-related factor 2 (Nrf2), which binds to antioxidant response element (ARE) in gene promoters and regulates the expression of antioxidant and phase II detoxifying enzyme genes (Ma, 2013).
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Recent studies have demonstrated that activation of aryl hydrocarbon receptor (AHR) could lead to the generation of ROS presumably by upregulating the expression of CYP metabolizing enzymes and the release of superoxide/hydrogen peroxide (Livak and Schmittgen, 2001; Takei et al., 2015; Zhou et al., 2017). On the other hand, AHR knockdown attenuated the increased ROS production by particulate matter (PM) and Benzo[a]pyrene (BaP) (Chiba et al., 2011; Harmon et al., 2018). The AHR is a
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member of the basic helix-loop-helix (bHLH) PAS family of transcription factors,
which can be activated not only by various external environmental agents but also by
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endogenous ligands (Kawajiri and Fujii-Kuriyama, 2017). In the canonical AHR activation pathway, activated AHR enters the nucleus and
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heterodimerizes with aryl hydrocarbon receptor nuclear translocator (ARNT). The
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ARNT/AHR complexes can then bind to dioxin response elements (DRE) to regulate the transcription of genes involved in xenobiotic metabolism such as phase I and
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phase II enzymes. AHR agonists such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and biphenyl 126 (PCB126) were reported to cause cardiac teratogenesis in zebrafish
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embryos (Jonsson et al., 2007; Teraoka et al., 2003; Wincent et al., 2015). Disruption
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of AHR-signalling activity perturbs cardiomyocyte differentiation in human embryonic stem cells (ES cells) and causes abnormal cardiac structure and function in zebrafish and mice (Carreira et al., 2015; Lanham et al., 2014; Wang et al., 2013b). In addition to oxidative stress, there is also a crosstalk between AHR and Nrf2 pathways. Nrf2 can be activated by a number of chemical activators of AHR including TCDD, 5
and the activation of Nrf2 target genes such as nqo1 require both Nrf2 and AHR (Ma et al., 2004; Rooney et al., 2018; Yeager et al., 2009). Till date, the molecular mechanisms of TCE-induced cardiac toxicity remain to be elucidated. Based on the previous findings mentioned above, we hypothesize that AHR-mediated oxidative stress contributes to the cardiac malformations induced by TCE. Zebrafish (Danio rerio) has long been used as a successful model to study the
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cardiac developmental toxicity of various stressors and toxicants (Brown et al., 2016; Sarmah and Marrs, 2016). While most mammals have only one ahr and nrf2, the
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zebrafish has multiple paralogs of ahr (ahr1a, ahr1b, ahr2) and nrf2 (nrf2a, nrf2b), of which ahr2 has been reported to mediate the effects of classic AHR ligands such as
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TCDD and PCB126 (Andreasen et al., 2002; Liu et al., 2016). In this study, we aimed
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to investigate the effects of AHR-induced oxidative stress on the cardiac developmental toxicity of TCE in zebrafish embryos. ROS and DNA damage levels,
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as well as the gene expression/activity of AHR and Nrf2 signalling genes were also examined in the heart of zebrafish embryos under different treatment conditions. The
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role of AHR was then confirmed by using Morpholino (MO)-mediated AHR
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knockdown.
2. Materials and methods 2.1 Chemicals
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Trichloroethylene (TCE, CAS 79-01-6), Trichloroacetic acid (TCA, CAS 76-03-9), Benzo [a] pyrene（BaP, CAS 50-32-8) and N-Acetyl-L-cysteine (NAC, CAS 616-91-1) were purchased from Adamas-Beta (Shanghai, China). CH223191 (CH, CAS 301326-22-7) and StemRegenin 1 (SR1, CAS 1227633-49-9) were obtained from MedChemExpress (Shanghai, China) and TargetMol (Shanghai, China), respectively. 2,4,3′,5′-tetramethoxystilbene (TMS, CAS 24144-92-1) was obtained from
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Topscience (Shanghai, China). All other chemicals were from Sigma-Aldrich (Shanghai, China) unless otherwise stated.
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2.2 Fish maintenance and embryo treatment
All experiments were conducted in accordance with the Institutional Animal
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Care/User Ethical Committee of Soochow University, Suzhou City, China. Mature
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wild-type adult zebrafish (AB-strain) were obtained from China Zebrafish Resource Centre (Wuhan, China) and maintained at 28.5 ± 0.5℃ in a zebrafish facility
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containing buffered water with a photoperiod of 14-hour light and 10-hour dark cycles. Sexually mature fish without any deformities or signs of disease were selected as
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breeders. Fertilized eggs were visually selected and only normally developed eggs
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were used for further testing. Eggs were then exposed to chemicals from 3-hour post-fertilization (hpf) in glass petri dishes with DMSO as a vehicle control. The solutions were renewed daily with fresh medium. Embryos at 72 hpf were transferred to concave microscope slides for the detection of cardiac abnormalities under a stereo
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microscope, and at least 100 embryos were examined for each group. The hearts were dissected and collected as described previously (Yue et al., 2017). 2.3 Quantitative Real-Time PCR (qPCR) Total RNA was extracted from isolated hearts (n=50) using TRNzol A+ reagent (Tiangen, Beijing, China), and 1 μg was used for cDNA synthesis by RevertAidTM First Strand cDNA Synthesis Kit (Thermo Scientific Fermentas, USA). qPCR was
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carried out using FS Universal SYBR Green Master mix (Roche, shanghai, China) in an ABI QuantStudio 6 qPCR system (Applied Biosystems). Primer sequences were
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listed in Table S1. The Ct values of target genes were normalized to those of gapdh and elfa, and the relative expression change was calculated using the comparative
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2.4 Antioxidant activity assays
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threshold cycle method (2-ΔΔCt) as described before (Livak and Schmittgen, 2001).
Whole embryo catalase (Cat) activity was measured by using a Catalase activity
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assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s instructions. The activities of total Sod, Sod1 and Sod2 in whole
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embryos were measured by using a Superoxide Dismutase Typed Assay Kit
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(Nanjing Jiancheng Bioengineering Institute, China). 2.5 In vivo Ethoxyresorufin-O-deethylase (EROD) assay Cyp1a1 activity was measured by a modified in vivo EROD assay (Le Bihanic et al., 2013). Briefly, embryos from each group were incubated in fish water with 0.4 μg/mL 7-ethoxyresorufin for 30 min. After washing for 3 mins, the embryos were 8
anesthetized by MS-222 and imaged by using a fluorescent microscope (Olympus IX73, Japan). The fluorescence intensity was quantified by using image J. At least 20 embryos were examined for each group. 2.6 ROS measurement The production of ROS was measured using dichlorodihydrofluorescein diacetate (DCFH-DA). Briefly, zebrafish (n=10 per group) were incubated with 20 μM
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DCFH-DA in the dark for 30 mins and then washed three times. Fluorescent
microscopic images were taken by using an Olympus IX73 microscope. At least 30
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embryos were examined for each group. 2.7 Oil-Red-O staining
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Oil-Red-O (ORO) staining was used to detect lipid distribution. Embryos were fixed
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in 4% paraformaldehyde (PFA) at 4°C overnight and then washed with 60% isopropanol for one hour. After incubating in 1 ml of ORO working solution (0.25%
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ORO stock in 60% isopropanol) for 1 hour, the embryos were washed twice with 60% isopropanol and photographed under a Nikon SMZ1500 zoom stereomicroscope
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group.
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(Nikon Corporation, Tokyo, Japan). At least 20 embryos were examined for each
2.8 Acridine Orange staining To detect cell apoptosis in the heart region, live embryos were stained with acridine orange (AO). Specifically, zebrafish embryos were washed with egg water three times and incubated with 5 μg/mL AO for 30 mins in the dark. The embryos were then 9
thoroughly washed with egg water again before being imaged under a fluorescent microscope (Olympus IX73, Olympus Corporation, Tokyo, Japan). At least 20 embryos were examined for each group. 2.9 Immunofluorescence Hearts dissected from embryos at 72 hpf were fixed with 4% PFA and permeabilized with 2% TritonX-100 before being blocked with 5% goat serum. After probing with
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primary antibodies against AHR (Immunoway Biotechnology, Plano, TX, USA) and
8-OHdG (sc-66036, Santa Cruz, EU) overnight at 4°C, the embryos were washed and
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incubated with secondary antibody labelled with Alexa Fluor 555 and Alexa Fluor
488 respectively (Invitrogen, Carlsbad, CA, USA). The distribution of fluorescence
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intensity was observed under a fluorescent microscopy. At least 15 embryos were
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examined for each group. 2.10 Morpholino Injection
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Morpholino (MO) antisense oligonucleotides were designed and produced by Gene Tools, LLC (Philomath, OR, USA). AHR knockdown was achieved by using the
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designed ahr2 MO (5'-TGTACCGATACCCGCCGACATGGTT-3') and Gene Tools’
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standard control MO (5'-CCTCTTACCTCAGTTACAATTTATA-3'). The MOs were re-suspended in RNase free water as a 3mM stock solution and diluted to 0.5 mM as the working solution for microinjections. Ahr2 MO and control MO were injected into zebrafish embryos at one-two cell stage with the micro-injector (Applied Scientific Instrumentation MPPI-3, Eugene, USA). 10
2.11 Statistical Analysis All experiments were performed at least three times, and results were represented as mean ± SEM. The statistical methods used were one-way ANOVA followed by Dunnett's or Turkey’s post-hoc test when appropriate. A P value of <0.05 was considered as statistically significant difference between groups.
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3. Results 3.1 Heart defects of zebrafish embryos
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As shown in Figure 1, TCE at 1 ppb (7.6 nM) had little effect on zebrafish heart development, but TCE at 10 ppb (76 nM) and 100 ppb (760 nM) significantly
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increased heart malformations such as balloon- shaped heart chambers and severe
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pericardial edema. TCA at 10 ppb (6 nM) also significantly increased heart defects, but to a lesser extent than that caused by TCE at the same concentration.
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Supplementation with AHR inhibitors, CH and SR1 significantly attenuated the heart defects in embryos exposed to TCE at 10 ppb, with the heart malformation rates being
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reduced to control levels by CH. It is worth noting that the ROS scavenger, NAC, also
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counteracted the EOM-induced cardiac malformations. Fish survival rate was only decreased by TCE at the highest dose level (100 ppb). 3.2 Expression and activity of AHR signaling genes We first tested the effect of TCE on the mRNA expression of genes involved in AHR signaling pathway including ahr2, ahrra, ahrrb, cyp1a1 and cyp1b1. As shown in 11
Figure 2A, only cyp1b1 was significantly upregulated by TCE, which was counteracted by AHR inhibitor, CH. We further found that TMS, a selective cyp1b1 inhibitor, partly attenuated the TCE-induced heart defects, with the malformation rates being dropped from 15.48 % to 10.06 % (Figure 2A). In the heart of zebrafish embryos, TCE had no detectable effects on either AHR protein level or EROD activity which mainly measures the activity of Cyp1a1 (Figure 2B and 2C). In
expression levels and EROD activity (Figures 2B and 2C).
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3.3 Oxidative stress
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contrast, the typical AHR agonist, BaP induced a significant increase in AHR
Treatment with TCE significantly increased ROS and 8-OHdG levels in the heart of
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zebrafish embryos, albeit to a lesser extent when compared to those induced by BaP
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(Figure 3). Both AHR inhibitor, CH and ROS scavenger, NAC, counteracted the effect of TCE on ROS and 8-OHdG (Figure 3). As shown in Figure 4, TCE had no
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effect on lipid consumption (ORO staining) or apoptosis (AO staining) in the heart of zebrafish embryos, while BaP significantly increased ORO and AO staining levels,
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indicating promotion of apoptosis and impaired lipid consumption.
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For the oxidative stress-related genes including cat, sod1, sod2, ho1, nqo1, gstp1, gstp2, nrf2a, nrf2b and keap1a, TCE downregulated nrf2b and upregulated sod2, ho1 and nqo1 (Figure 5A). In samples exposed to TCE plus CH, gstp2, sod2, ho1, nqo1 returned to control gene expression levels while nrf2b was even overexpressed (Figure 5A). TCE had no effect on Cat, but the activities of total Sod, Sod1 and Sod2 12
were all increased in embryos exposed to TCE and then attenuated by CH supplementation (Figure 5B). 3.4 Expression of genes essential to heart development We examined the effect of TCE on genes essential to cardiac differentiation. As demonstrated in Figure 6, TCE decreased the mRNA levels of gata4 and hand2 increased those of c-fos and sox9b, which were significantly attenuated in the heart of
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embryos exposed to TCE plus CH. However, TCE had no effect on the expression levels of nkx2.5 and axin2 (Figure 6).
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3.5 Effects of AHR knockdown
As expected, AHR knockdown by MO significantly attenuated TCE-induced heart
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defects (Figure S1). Although EROD activity was not affected by TCE treatment, its
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background level was significantly reduced by AHR knockdown (Figure 7A). Similar effects were observed for the expression levels of AHR protein and Cyp1a1 mRNA
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(Figure S2). As shown in Figure 7B and 7C, AHR knockdown with morpholino
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significantly attenuated the TCE-induced upregulation of ROS and 8-OHdG levels.
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4. Discussion
Increasing evidence indicates that TCE has the potential to cause cardiac malformations, but the underlining mechanism(s) remain unclear (Caldwell et al., 2008; Drake et al., 2006; Makris et al., 2016; Rufer et al., 2010; Wirbisky et al., 2016). In early development, oxidative stress can trigger the signal pathways 13
involved in cellular functions such as cell proliferation, differentiation and apoptosis (Asoglu et al., 2018). In addition, elevated ROS has been found to alter the expression of genes crucial to the differentiation of cardiac neural crest cells, leading to heart defects during embryonic development (Asoglu et al., 2018; Dennery, 2007). In this study, we found that TCE elevated ROS levels and 8-OHdG signals (a well-known marker of oxidative stress and DNA damage) in the heart of zebrafish
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embryos. In addition, the TCE-induced heart defects were significantly counteracted
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by the ROS scavenger, NAC.
Oxidative stress has also been known to induce lipid peroxidation leading to
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disordered lipid distribution (Hauck and Bernlohr, 2016; Huang et al., 2018).
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However, changes in lipid distribution were only detected in the heart of embryos exposed to BaP but not to TCE. Considering that the ROS level induced by TCE was
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much lower than that by BaP in our study, we hypothesize that the TCE-induced
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oxidative stress may not have been robust enough to affect lipid metabolism.
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Nrf2 is the main regulator of the adaptive response to oxidative stress. Elevated ROS levels lead to the oxidation of Keap1 and the release of Nrf2, which then translocates to the nucleus and activates the transcription of various antioxidant and phase II detoxification genes. Zebrafish has two nrf2 genes, nrf2a and nrf2b, with the later showing negative regulatory effects during development (Rousseau et al., 2015). In 14
our study, TCE had no effect on nrf2a mRNA expression, but the mRNA expression level of nrf2b was significantly decreased in the heart of zebrafish embryos exposed to TCE. Consistent with these observations, ho1, the direct target genes of nrf2b, was upregulated by TCE. In addition, the mRNA expression levels of nrf2a downstream genes, including sod2, gstp2 and nqo1, were also upregulated, with increased enzyme activities of total Sod, Sod1 and Sod2, indicating that Nrf2a was activated
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through a post-transcriptional mechanism in the heart of zebrafish embryos exposed to TCE. Ho1, Nqo1, Sod1/2 and Gstp2 all have antioxidant effects, but
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overexpression of these antioxidant enzymes and high levels of ROS are frequent findings in TCE-exposed samples (Biswas et al., 2014; Klotz and Steinbrenner,
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2017). One possible explanation is that the overexpression changes of the four genes
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TCE-induced ROS.
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are only 1.5-2.5 times in magnitude, which may not be enough to scavenge
Because we found that both AHR inhibitors CH and SR1 protected zebrafish
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embryos from the cardiac developmental toxicity of TCE, we rationalized that AHR
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might play an essential role in the TCE-induced heart defects. Our MO knockdown experiments further confirmed the role of AHR in the cardiac developmental toxicity of TCE. Moreover, the TCE-induced ROS and 8-OHdG signals were abolished by AHR inhibition via either supplementation of CH or by gene knockdown. CH also attenuated the TCE-induced mRNA expression changes of genes essential to heart 15
development including gata4, hand2, c-fos and sox9b. It should be noted that we only used ahr2 MO to knockdown AHR, indicating that ahr2 is responsible for the toxic effects of TCE. However, we cannot rule out the possibility that ahr1a/1b can also play a role in the cardiac developmental toxicity of TCE.
Given the known structural requirements for binding and activating DREs generated
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by Casiewicz et al, TCE is not expected to activate canonical AHR signaling
(Gasiewicz et al., 1996). Consistent with this, TCE cannot active DRE in a human
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cell-based luciferase reporter gene assay from the Tox21 database (NCBI, 2014). However, recent studies also revealed non-canonical AHR signaling pathways,
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during which AHR interacts with different ligands and modulates transcription
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through sites distinct from DRE (Wright et al., 2017). AHR can also bind to, and be activated by a variety of chemicals with structures dramatically different from the
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classic AHR ligands such as PAHs (polyaromatic aromatic hydrocarbons) and HAHs (halogenated aromatic hydrocarbons), and a number of endogenous AHR ligands
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have been identified (Kawajiri and Fujii-Kuriyama, 2017). Ethanol, which does not
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meet the structural requirements just like TCE, has been reported to activate AHR in mouse hepatic stellate cells (Zhang et al., 2012). In addition, ultraviolet B (UVB) can activate AHR by generating the tryptophan photoproduct, 6-Formylindolo [3,2-b] carbazole (FICZ), which serves as an endogenous AHR agonist (Furue et al., 2019). Thus, we hypothesize that TCE might activate AHR by generating endogenous 16
ligands in the heart of zebrafish embryos.
It has been suggested that AHR can induce oxidative stress by upregulating Cyp1a1 (Livak and Schmittgen, 2001), yet neither the cyp1a1 mRNA expression nor its activity as measured by the EROD assay was upregulated by TCE in this study. Nevertheless, cyp1b1 was overexpressed in the heart of zebrafish embryos exposed to
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TCE. It has been reported that Cyp1b1 plays a direct role in PAH-mediated oxidative damage (Green et al., 2008). Cyp1b1 can induce ROS generation by bioactivating
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xenobiotics and activating NADPH oxidase (Green et al., 2008; Yaghini et al., 2010). Thus, Cyp1b1 instead of Cyp1a1 might mediate the TCE-induced oxidative stress in
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the heart of zebrafish embryos, which is consistent with our observations that the
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selective Cyp1b1 inhibitor, TMS, partly attenuated the TCE-induced heart defects.
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Cyp1a1 is a classical AHR target gene, but recent studies suggest that different AHR ligands can induce distinct ligand specific sets of AHR-dependent genes (Denison and
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Faber, 2017). Activation of AHR can even form heterodimers with nuclear proteins
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other than ARNT to regulate the expression of genes lacking DRE (Denison and Faber, 2017). It will be interesting to find out if other small organic chemicals which do not possess the classic structure of typical AHR agonists can also activate AHR dependent genes in a similar manner as TCE.
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A crosstalk between AHR and Nrf2 has been well documented (Haarmann-Stemmann et al., 2012; Kohle and Bock, 2007; Yeager et al., 2009; Zgheib et al., 2018). The AHR agonist, TCDD, can increase Nrf2 protein level and activate Nrf2 activity in mice (Wang et al., 2013a). The presence of multiple potential DREs in the promoter and first intron of nrf2a and nrf2b indicates that their expression levels might be regulated by AHR (Rousseau et al., 2015). Timme et al
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found that Nrf2b instead of Nrf2a was induced by TCDD in an AHR dependent
manner in zebrafish embryos, prompting a higher sensitivity of Nrf2b to AHR than
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that of Nrf2a (Timme-Laragy et al., 2012). In this study, we found that AHR
inhibitor, CH, counteracted the TCE-induced downregulation of Nrf2b. The mRNA
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expression level of ho1, a direct Nrf2b target gene, was also upregulated by TCE and
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restored by TCE plus CH. It is worth noting that in our study, Nrf2b is repressed rather than activated by AHR, which is contrary to a previous report (Timme-Laragy
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et al., 2012). This may be due to differences in inducers, doses, exposure duration or some other factors. Although TCE had no effect on nrf2a mRNA expression, TCE
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upregulated the mRNA expression levels and activities of Nrf2a downstream genes,
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which were attenuated by CH supplementation, suggesting that AHR mediates the activation of Nrf2a induced by TCE at a post-transcriptional level. Future studies would be needed to quantify the effect of TCE on Nrf2a protein levels.
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TCE is metabolized in vivo via two major pathways: cytochrome P450 oxidation pathway and glutathione (GSH) synthesis pathway. TCA, the main oxidative pathway metabolite of TCE, has been reported to increase the incidence of cardiac defects (Johnson et al., 1998a, b; Smith et al., 1989). However, in our study, the TCA-induced heart defects were of much lower severity than the equivalent defects caused by TCE. Instead, we found that the mRNA expression level of glutathione transferase gstp2
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was increased, indicating that GSH synthesis pathway might play a crucial role in the cardiac developmental toxicity of TCE. Future studies are warranted to examine the
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the cardiac development of zebrafish embryos.
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effects of GSH synthesis pathways and the metabolites of TCE (such as DCVC) on
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5. Conclusions
Our study indicates that in the heart of zebrafish embryos, TCE can activate AHR,
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which could enhance ROS generation via Cyp1b1 overexpression, leading to cardiac defects. Most significantly, AHR activation is a necessary step for the ROS-induced
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Nrf2-antioxidant response (See Figure 8). To the best of our knowledge, this is the
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first study showing that AHR plays an important role in the cardiac developmental toxicity of TCE. Given the abundance of natural AHR antagonists and antioxidants in food, our results provide the scientific basis for the selection of candidate entities for possible development as agents for use in the prevention or treatment of TCE-induced CHDs. 19
Declarations of interest: None Conflict of interest statement All authors declare no conflict of interest.
Acknowledgments This work was supported by the National Nature Sciences Foundation of China (Grant
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number: 81570284), Applied Basic Research in Suzhou (Grant number: sys201519) and The Priority Academic Program Development of Jiangsu Higher Education
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Institutions. We thank Prof. Han Wang for his help in preparing zebrafish embryos.
References
Agency for Toxic Substances and Disease Registry (ATSDR). 2017. ATSDR’s Substance Priority List.
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https://www.atsdr.cdc.gov/spl/index.html [accessed 9 May 2019]
Andreasen, E.A., Spitsbergen, J.M., Tanguay, R.L., Stegeman, J.J., Heideman, W., Peterson, R.E., 2002. Tissue-specific expression of AHR2, ARNT2, and CYP1A in zebrafish embryos and larvae: effects of
na
developmental stage and 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure. Toxicol Sci 68, 403-419. Asoglu, M.R., Gabbay-Benziv, R., Turan, O.M., Turan, S., 2018. Exposure of the developing heart to diabetic environment and early cardiac assessment: A review. Echocardiography 35, 244-257. Biswas, C., Shah, N., Muthu, M., La, P., Fernando, A.P., Sengupta, S., Yang, G., Dennery, P.A., 2014.
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Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses. J Biol Chem 289, 26882-26894. Blossom, S.J., Doss, J.C., Hennings, L.J., Jernigan, S., Melnyk, S., James, S.J., 2008. Developmental
Jo
exposure to trichloroethylene promotes CD4+ T cell differentiation and hyperactivity in association with oxidative stress and neurobehavioral deficits in MRL+/+ mice. Toxicol Appl Pharmacol 231, 344-353.
Blossom, S.J., Melnyk, S.B., Li, M., Wessinger, W.D., Cooney, C.A., 2017. Inflammatory and oxidative stress-related effects associated with neurotoxicity are maintained after exclusively prenatal trichloroethylene exposure. Neurotoxicology 59, 164-174. Brown, D.R., Samsa, L.A., Qian, L., Liu, J., 2016. Advances in the Study of Heart Development and Disease Using Zebrafish. J Cardiovasc Dev Dis 3.
20
Caldwell, P.T., Thorne, P.A., Johnson, P.D., Boitano, S., Runyan, R.B., Selmin, O., 2008. Trichloroethylene disrupts cardiac gene expression and calcium homeostasis in rat myocytes. Toxicol Sci 104, 135-143. Carreira, V.S., Fan, Y., Kurita, H., Wang, Q., Ko, C.I., Naticchioni, M., Jiang, M., Koch, S., Zhang, X., Biesiada, J., Medvedovic, M., Xia, Y., Rubinstein, J., Puga, A., 2015. Disruption of Ah Receptor Signaling during Mouse Development Leads to Abnormal Cardiac Structure and Function in the Adult. PLoS One 10, e0142440. Chiba, T., Uchi, H., Tsuji, G., Gondo, H., Moroi, Y., Furue, M., 2011. Arylhydrocarbon receptor (AhR) activation in airway epithelial cells induces MUC5AC via reactive oxygen species (ROS) production. Pulmonary Pharmacology & Therapeutics 24, 133-140. Denison, M.S., Faber, S.C., 2017. And Now for Something Completely Different: Diversity in Ligand-Dependent Activation of Ah Receptor Responses. Curr Opin Toxicol 2, 124-131.
ro of
Dennery, P.A., 2007. Effects of oxidative stress on embryonic development. Birth Defects Res C Embryo Today 81, 155-162.
Drake, V.J., Koprowski, S.L., Hu, N., Smith, S.M., Lough, J., 2006. Cardiogenic effects of
trichloroethylene and trichloroacetic acid following exposure during heart specification of avian development. Toxicol Sci 94, 153-162.
-p
United States Environmental Protection Agency (US EPA). Toxicological Review of Trichloroethylene. Available: https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=215006#tab-3 [accessed 9 May 2019].
re
Furue, M., Uchi, H., Mitoma, C., Hashimoto-Hachiya, A., Tanaka, Y., Ito, T., Tsuji, G., 2019. Implications of tryptophan photoproduct FICZ in oxidative stress and terminal differentiation of keratinocytes. G Ital Dermatol Venereol 154, 37-41.
lP
Gasiewicz, T.A., Kende, A.S., Rucci, G., Whitney, B., Willey, J.J., 1996. Analysis of structural requirements for Ah receptor antagonist activity: ellipticines, flavones, and related compounds. Biochem Pharmacol 52, 1787-1803.
Green, R.M., Hodges, N.J., Chipman, J.K., O'Donovan, M.R., Graham, M., 2008. Reactive oxygen
na
species from the uncoupling of human cytochrome P450 1B1 may contribute to the carcinogenicity of dioxin-like polychlorinated biphenyls. Mutagenesis 23, 457-463. Haarmann-Stemmann, T., Abel, J., Fritsche, E., Krutmann, J., 2012. The AhR-Nrf2 pathway in
ur
keratinocytes: on the road to chemoprevention? J Invest Dermatol 132, 7-9. Harmon, A.C., Hebert, V.Y., Cormier, S.A., Subramanian, B., Reed, J.R., Backes, W.L., Dugas, T.R., 2018. Particulate matter containing environmentally persistent free radicals induces AhR-dependent
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cytokine and reactive oxygen species production in human bronchial epithelial cells. PLoS One 13, e0205412.
Hauck, A.K., Bernlohr, D.A., 2016. Oxidative stress and lipotoxicity. J Lipid Res 57, 1976-1986. Huang, M., Jiao, J., Wang, J., Xia, Z., Zhang, Y., 2018. Characterization of acrylamide-induced oxidative stress and cardiovascular toxicity in zebrafish embryos. J Hazard Mater 347, 451-460. Jiang, Y., Wang, D., Zhang, G., Wang, G., Tong, J., Chen, T., 2016. Disruption of cardiogenesis in human embryonic stem cells exposed to trichloroethylene. Environ Toxicol 31, 1372-1380. Johnson, P.D., Dawson, B.V., Goldberg, S.J., 1998a. Cardiac teratogenicity of trichloroethylene metabolites. J Am Coll Cardiol 32, 540-545. 21
Johnson, P.D., Dawson, B.V., Goldberg, S.J., 1998b. A review: trichloroethylene metabolites: potential cardiac teratogens. Environ Health Perspect 106 Suppl 4, 995-999. Jonsson, M.E., Jenny, M.J., Woodin, B.R., Hahn, M.E., Stegeman, J.J., 2007. Role of AHR2 in the expression of novel cytochrome P450 1 family genes, cell cycle genes, and morphological defects in developing zebra fish exposed to 3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci 100, 180-193. Kawajiri, K., Fujii-Kuriyama, Y., 2017. The aryl hydrocarbon receptor: a multifunctional chemical sensor for host defense and homeostatic maintenance. Exp Anim 66, 75-89. Klotz, L.O., Steinbrenner, H., 2017. Cellular adaptation to xenobiotics: Interplay between xenosensors, reactive oxygen species and FOXO transcription factors. Redox Biol 13, 646-654. Kohle, C., Bock, K.W., 2007. Coordinate regulation of Phase I and II xenobiotic metabolisms by the Ah receptor and Nrf2. Biochem Pharmacol 73, 1853-1862.
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Lanham, K.A., Plavicki, J., Peterson, R.E., Heideman, W., 2014. Cardiac myocyte-specific AHR activation phenocopies TCDD-induced toxicity in zebrafish. Toxicol Sci 141, 141-154.
Lash, L.H., Fisher, J.W., Lipscomb, J.C., Parker, J.C., 2000. Metabolism of trichloroethylene. Environ Health Perspect 108 Suppl 2, 177-200.
Le Bihanic, F., Couillard, C.M., Rigaud, C., Legare, B., 2013. A simple and reliable in vivo EROD activity
-p
measurement in single Fundulus heteroclitus embryo and larva. Mar Environ Res 84, 17-23.
Liu, H., Nie, F.H., Lin, H.Y., Ma, Y., Ju, X.H., Chen, J.J., Gooneratne, R., 2016. Developmental toxicity, EROD, and CYP1A mRNA expression in zebrafish embryos exposed to dioxin-like PCB126. Environ
re
Toxicol 31, 201-210.
Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402-408.
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Ma, Q., 2013. Role of nrf2 in oxidative stress and toxicity. Annu Rev Pharmacol Toxicol 53, 401-426. Ma, Q., Kinneer, K., Bi, Y., Chan, J.Y., Kan, Y.W., 2004. Induction of murine NAD(P)H:quinone oxidoreductase by 2,3,7,8-tetrachlorodibenzo-p-dioxin requires the CNC (cap 'n' collar) basic leucine zipper transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2): cross-interaction
na
between AhR (aryl hydrocarbon receptor) and Nrf2 signal transduction. Biochem J 377, 205-213. Makris, S.L., Scott, C.S., Fox, J., Knudsen, T.B., Hotchkiss, A.K., Arzuaga, X., Euling, S.Y., Powers, C.M., Jinot, J., Hogan, K.A., Abbott, B.D., Hunter, E.S., 3rd, Narotsky, M.G., 2016. A systematic evaluation of
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the potential effects of trichloroethylene exposure on cardiac development. Reprod Toxicol 65, 321-358.
NCBI, 2014. NCBI National Center for Biotechnology Information . PubChem BioAssay Database;
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AID=743122, .
Rooney, J.P., Oshida, K., Kumar, R., Baldwin, W.S., Corton, J.C., 2018. Chemical Activation of the Constitutive Androstane Receptor (CAR) Leads to Activation of Oxidant-Induced Nrf2. Toxicol Sci. Rousseau, M.E., Sant, K.E., Borden, L.R., Franks, D.G., Hahn, M.E., Timme-Laragy, A.R., 2015. Regulation of Ahr signaling by Nrf2 during development: Effects of Nrf2a deficiency on PCB126 embryotoxicity in zebrafish (Danio rerio). Aquat Toxicol 167, 157-171. Rufer, E.S., Hacker, T.A., Flentke, G.R., Drake, V.J., Brody, M.J., Lough, J., Smith, S.M., 2010. Altered cardiac function and ventricular septal defect in avian embryos exposed to low-dose trichloroethylene. Toxicol Sci 113, 444-452. 22
Sarmah, S., Marrs, J.A., 2016. Zebrafish as a Vertebrate Model System to Evaluate Effects of Environmental Toxicants on Cardiac Development and Function. Int J Mol Sci 17. Smith, M.K., Randall, J.L., Read, E.J., Stober, J.A., 1989. Teratogenic activity of trichloroacetic acid in the rat. Teratology 40, 445-451. Takei, K., Hashimoto-Hachiya, A., Takahara, M., Tsuji, G., Nakahara, T., Furue, M., 2015. Cynaropicrin attenuates UVB-induced oxidative stress via the AhR-Nrf2-Nqo1 pathway. Toxicol Lett 234, 74-80. Teraoka, H., Dong, W., Tsujimoto, Y., Iwasa, H., Endoh, D., Ueno, N., Stegeman, J.J., Peterson, R.E., Hiraga, T., 2003. Induction of cytochrome P450 1A is required for circulation failure and edema by 2,3,7,8-tetrachlorodibenzo-p-dioxin in zebrafish. Biochem Biophys Res Commun 304, 223-228. Timme-Laragy, A.R., Karchner, S.I., Franks, D.G., Jenny, M.J., Harbeitner, R.C., Goldstone, J.V., McArthur, A.G., Hahn, M.E., 2012. Nrf2b, novel zebrafish paralog of oxidant-responsive transcription factor NF-E2-related factor 2 (NRF2). J Biol Chem 287, 4609-4627.
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Toraason, M., Clark, J., Dankovic, D., Mathias, P., Skaggs, S., Walker, C., Werren, D., 1999. Oxidative stress and DNA damage in Fischer rats following acute exposure to trichloroethylene or perchloroethylene. Toxicology 138, 43-53.
Wang, L., He, X., Szklarz, G.D., Bi, Y., Rojanasakul, Y., Ma, Q., 2013a. The aryl hydrocarbon receptor interacts with nuclear factor erythroid 2-related factor 2 to mediate induction of
-p
NAD(P)H:quinoneoxidoreductase 1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Arch Biochem Biophys 537, 31-38.
Wang, Q., Chen, J., Ko, C.I., Fan, Y., Carreira, V., Chen, Y., Xia, Y., Medvedovic, M., Puga, A., 2013b.
re
Disruption of aryl hydrocarbon receptor homeostatic levels during embryonic stem cell differentiation alters expression of homeobox transcription factors that control cardiomyogenesis. Environ Health Perspect 121, 1334-1343.
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Wei, H., Cong, X., 2018. The effect of reactive oxygen species on cardiomyocyte differentiation of pluripotent stem cells. Free Radic Res 52, 150-158.
Wincent, E., Stegeman, J.J., Jonsson, M.E., 2015. Combination effects of AHR agonists and Wnt/beta-catenin modulators in zebrafish embryos: Implications for physiological and toxicological
na
AHR functions. Toxicol Appl Pharmacol 284, 163-179.
Wirbisky, S.E., Damayanti, N.P., Mahapatra, C.T., Sepulveda, M.S., Irudayaraj, J., Freeman, J.L., 2016. Mitochondrial Dysfunction, Disruption of F-Actin Polymerization, and Transcriptomic Alterations in
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Zebrafish Larvae Exposed to Trichloroethylene. Chem Res Toxicol 29, 169-179. Wright, E.J., Pereira De Castro, K., Joshi, A.D., Elferink, C.J., 2017. Canonical and non-canonical aryl hydrocarbon receptor signaling pathways. Current Opinion in Toxicology 2, 87-92.
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Yaghini, F.A., Song, C.Y., Lavrentyev, E.N., Ghafoor, H.U., Fang, X.R., Estes, A.M., Campbell, W.B., Malik, K.U., 2010. Angiotensin II-induced vascular smooth muscle cell migration and growth are mediated by cytochrome P450 1B1-dependent superoxide generation. Hypertension 55, 1461-1467. Yeager, R.L., Reisman, S.A., Aleksunes, L.M., Klaassen, C.D., 2009. Introducing the "TCDD-inducible AhR-Nrf2 gene battery". Toxicol Sci 111, 238-246. Yue, C., Ji, C., Zhang, H., Zhang, L.W., Tong, J., Jiang, Y., Chen, T., 2017. Protective effects of folic acid on PM2.5-induced cardiac developmental toxicity in zebrafish embryos by targeting AhR and Wnt/beta-catenin signal pathways. Environ Toxicol 32, 2316-2322.
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Zgheib, E., Limonciel, A., Jiang, X., Wilmes, A., Wink, S., van de Water, B., Kopp-Schneider, A., Bois, F.Y., Jennings, P., 2018. Investigation of Nrf2, AhR and ATF4 Activation in Toxicogenomic Databases. Front Genet 9, 429. Zhang, H.F., Lin, X.H., Yang, H., Zhou, L.C., Guo, Y.L., Barnett, J.V., Guo, Z.M., 2012. Regulation of the activity and expression of aryl hydrocarbon receptor by ethanol in mouse hepatic stellate cells. Alcohol Clin Exp Res 36, 1873-1881. Zhou, B., Wang, X., Li, F., Wang, Y., Yang, L., Zhen, X., Tan, W., 2017. Mitochondrial activity and oxidative stress functions are influenced by the activation of AhR-induced CYP1A1 overexpression in
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cardiomyocytes. Mol Med Rep 16, 174-180.
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Figure Legends: Figure 1. Cardiac defects and survival rate of zebrafish embryos at 72 hpf. A) Represent images of zebrafish embryos at 72 hpf. Dotted lines encircle atrium (red) or ventricle (blue). TCE1, 10, 100: Trichloroethylene at 1 ppb, 10 ppb, 100 ppb; TCA10: Trichloroacetic acid at 10 ppb; BaP: Benzo[a]pyrene at 0.1 μM; CH: AHR inhibitor, CH223191 at 0.125 μM; SR1: AHR inhibitor, StemRegenin 1 at 0.25 μM; NAC: ROS
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scavenger, N-Acetyl-L-cysteine at 0.25 μM. B) Heart malformation rates. C) Survival rates. Different letters (a-c) indicate significant differences.
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Figure 2. Expression and activity of AHR signaling genes in the heart of
zebrafish embryos at 72 hpf. A) mRNA expression and heart malformation rates.
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TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at 0.125 μM; B)
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Immunostaining of AHR and the relevant quantification results. BaP: Benzo[a]pyrene at 0.1 μM; NAC: ROS scavenger, N-Acetyl-L-cysteine at 0.25 μM. Different letters
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(a-b) indicate significant differences. C) Representative images and quantification results of Ethoxyresorufin-O-deethylase (EROD) activity (a catalytic measurement of
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Cyp1a1 induction). The dotted areas indicate heart regions; ***, p<0.001.
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Figure 3. ROS and 8-OHdG signals in the heart of zebrafish embryos at 72 hpf. A) ROS signal and the quantification results. The dotted areas indicate heart regions. B) Immunostaining of 8-OhdG and the relevant quantification results. TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at 0.125 μM; NAC: ROS
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scavenger, N-Acetyl-L-cysteine at 0.25 μM; BaP: Benzo[a]pyrene at 0.1 μM. Different letters (a-c) indicate significant differences. Figure 4. Lipid distribution and apoptosis in the heart of zebrafish embryos at 72 hpf. A) Representative images of lipid distribution stained by Oil-Red-O and the quantification result. B) Representative images of apoptosis stained by acridine orange and the quantification result. i) Whole fish; ii) Enlarged pictures showing the
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heart region. Dotted lines encircle atrium (red) or ventricle (blue). TCE10,
Trichloroethylene at 10 ppb; BaP: Benzo[a]pyrene at 0.1 μM. ***, p<0.001.
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Figure 5. Oxidative stress related gene expression/activity levels in the heart of
zebrafish embryos at 72 hpf. A) mRNA expression changes. B) Catalase and SOD
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activities. TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at
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0.125 μM; Different letters (a-c) indicate significant differences. Figure 6. mRNA expression changes of genes essential to heart development in
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zebrafish embryos at 72 hpf. TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at 0.125 μM; Different letters (a-c) indicate significant
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differences.
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Figure 7. EROD activity, ROS level and AHR/8-OHdG levels in AHR knockdown zebrafish embryos at 72 hpf (n≥5). A) Ethoxyresorufin-O-deethylase (EROD) activity. B) Reactive oxygen species (ROS) signal. C) Immunostaining of 8-OHdG and the quantification results. The dotted areas indicate heart regions.
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TCE10, Trichloroethylene at 10 ppb; ConMO: non-specific control morpholino; AHRMO: ahr2 morpholino. Different letters (a-c) indicate significant differences. Figure 8. Model showing that AHR-mediated oxidative stress contributes to TCE cardiac developmental toxicity. TCE-activated aryl hydrocarbon receptor (AHR) increases reactive oxygen species (ROS) via cyp1b1 overexpression, leading to heart malformations. ROS in turn activates Nrf2 (Nrf2a/2b) antioxidant signal
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pathway, and AHR activity is required for the regulation of Nrf2 target genes.
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Inhibition of AHR or ROS counteracts TCE-induced heart defects.
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PII:
S0304-3894(19)31475-X
DOI:
https://doi.org/10.1016/j.jhazmat.2019.121521
Reference:
HAZMAT 121521
To appear in:
Journal of Hazardous Materials
Received Date:
21 May 2019
Revised Date:
14 October 2019
Accepted Date:
21 October 2019
Please cite this article as: Jin H, Ji C, Ren F, Aniagu S, Tong J, Jiang Y, Chen T, AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos, Journal of Hazardous Materials (2019), doi: https://doi.org/10.1016/j.jhazmat.2019.121521
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2019 Published by Elsevier.
AHR-Mediated Oxidative Stress Contributes to the Cardiac Developmental Toxicity of Trichloroethylene in Zebrafish Embryos
Hongmei Jin1,#, Cheng Ji1,#, Fei Ren1,2, Stanley Aniagu3, Jian Tong1,2 , Yan Jiang1, *, and Tao Chen1,2,*
Medical College of Soochow University, Suzhou, P.R. China.
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Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric
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Diseases, Soochow University, Suzhou, China.
Toxicology, Risk Assessment and Research Division, Texas Commission on
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Environmental Quality, 12015 Park 35 Cir, Austin, TX, USA
Corresponding authors:
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*
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# These two authors contributed equally to the paper.
Dr. Yan Jiang, Medical College of Soochow University, Soochow University, 199
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Ren-Ai Road, Suzhou China. 215123; Email: [email protected]
Dr. Tao Chen, Medical College of Soochow University, Soochow University, 199 Ren-Ai Road, Suzhou China. 215123; Email: [email protected]
Graphical abstract
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Highlights:
AHR inhibition significantly counteracted the TCE-induced heart defects.
TCE upregulated the expression levels of cyp1b1 but not cyp1a1.
TCE-induced ROS production led to heart defects in zebrafish embryos.
AHR mediated the TCE-induced oxidative stress.
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Abstract
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Trichloroethylene (TCE), a widely used chlorinated solvent, is a common environmental pollutant. Current evidence shows that TCE could induce heart defects during embryonic development, but the underlining mechanism(s) remain unclear. Since activation of the aryl hydrocarbon receptor (AHR) could induce oxidative stress, we hypothesized that AHR-mediated oxidative stress may play a role in the cardiac developmental toxicity of TCE. In this study, we found that the 2
reactive oxygen species (ROS) scavenger, N-Acetyl-L-cysteine (NAC), and AHR inhibitors, CH223191 (CH) and StemRegenin 1, significantly counteracted the TCE-induced heart malformations in zebrafish embryos. Moreover, both CH and NAC suppressed TCE-induced ROS and 8-OHdG (8-hydroxy-2' -deoxyguanosine). TCE did not affect ahr2 and cyp1a expression, but increased cyp1b1 expression, which was restored by CH supplementation. CH also attenuated the TCE-induced
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mRNA expression changes of Nrf2 signalling genes (nrf2b, gstp2, sod2, ho1, nqo1) and cardiac differentiation genes (gata4, hand2, c-fos, sox9b). In addition, the TCE
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enhanced SOD activity was attenuated by CH. Morpholino knockdown confirmed that AHR mediated the TCE-induced ROS and 8-OHdG generation in the heart of
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zebrafish embryos. In conclusion, our results suggest that AHR mediates
zebrafish embryos.
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TCE-induced oxidative stress, leading to DNA damage and heart malformations in
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Keyword：Trichloroethylene; Aryl Hydrocarbon Receptor; Reactive Oxygen Species;
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Heart development; Zebrafish embryos
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1. Introduction
Trichloroethylene (TCE) is a widely used volatile organic compound (VOC) that has been frequently detected in air, groundwater and soil (EPA, 2011). It is ranked 16th in the priority list of hazardous substances in the Comprehensive Environmental Response, Compensation, and Liability Act (CERCLA) (ATSDR, 2007). 3
Epidemiological studies have linked maternal TCE exposure to spontaneous abortions, low birth weight, and congenital heart defects (CHDs) (Makris et al., 2016). Reports from animal experiments also showed various cardiac defects induced by TCE in avians, rats and zebrafish (Caldwell et al., 2008; Drake et al., 2006; Rufer et al., 2010; Wirbisky et al., 2016). In addition, we previously demonstrated that TCE inhibited cardiac differentiation in human stem cells (Jiang et al., 2016). After absorption, TCE
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is metabolized through two main pathways: Cytochrome P450 (CYP)-dependent
oxidation and glutathione (GSH) conjugation (Lash et al., 2000). It is worth noting
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that trichloroacetic acid (TCA), the main oxidative metabolite of TCE in human, was reported to induce cardiac defects in rats (Johnson et al., 1998b).
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Previously, there were several studies indicating that TCE treatment could induce
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oxidative stress in human cell lines as well as in animal models such as mice and rats (Blossom et al., 2008; Blossom et al., 2017; Toraason et al., 1999). Oxidative stress
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has been suggested to play a crucial role in the pathophysiology of CHDs (Asoglu et al., 2018). In addition, reactive oxygen species (ROS) has also been reported to be
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involved in cardiomyocyte differentiation of pluripotent stem cells (Wei and Cong,
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2018). The adaptive response to oxidative stress is mainly regulated by nuclear factor erythroid 2-related factor 2 (Nrf2), which binds to antioxidant response element (ARE) in gene promoters and regulates the expression of antioxidant and phase II detoxifying enzyme genes (Ma, 2013).
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Recent studies have demonstrated that activation of aryl hydrocarbon receptor (AHR) could lead to the generation of ROS presumably by upregulating the expression of CYP metabolizing enzymes and the release of superoxide/hydrogen peroxide (Livak and Schmittgen, 2001; Takei et al., 2015; Zhou et al., 2017). On the other hand, AHR knockdown attenuated the increased ROS production by particulate matter (PM) and Benzo[a]pyrene (BaP) (Chiba et al., 2011; Harmon et al., 2018). The AHR is a
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member of the basic helix-loop-helix (bHLH) PAS family of transcription factors,
which can be activated not only by various external environmental agents but also by
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endogenous ligands (Kawajiri and Fujii-Kuriyama, 2017). In the canonical AHR activation pathway, activated AHR enters the nucleus and
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heterodimerizes with aryl hydrocarbon receptor nuclear translocator (ARNT). The
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ARNT/AHR complexes can then bind to dioxin response elements (DRE) to regulate the transcription of genes involved in xenobiotic metabolism such as phase I and
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phase II enzymes. AHR agonists such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and biphenyl 126 (PCB126) were reported to cause cardiac teratogenesis in zebrafish
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embryos (Jonsson et al., 2007; Teraoka et al., 2003; Wincent et al., 2015). Disruption
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of AHR-signalling activity perturbs cardiomyocyte differentiation in human embryonic stem cells (ES cells) and causes abnormal cardiac structure and function in zebrafish and mice (Carreira et al., 2015; Lanham et al., 2014; Wang et al., 2013b). In addition to oxidative stress, there is also a crosstalk between AHR and Nrf2 pathways. Nrf2 can be activated by a number of chemical activators of AHR including TCDD, 5
and the activation of Nrf2 target genes such as nqo1 require both Nrf2 and AHR (Ma et al., 2004; Rooney et al., 2018; Yeager et al., 2009). Till date, the molecular mechanisms of TCE-induced cardiac toxicity remain to be elucidated. Based on the previous findings mentioned above, we hypothesize that AHR-mediated oxidative stress contributes to the cardiac malformations induced by TCE. Zebrafish (Danio rerio) has long been used as a successful model to study the
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cardiac developmental toxicity of various stressors and toxicants (Brown et al., 2016; Sarmah and Marrs, 2016). While most mammals have only one ahr and nrf2, the
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zebrafish has multiple paralogs of ahr (ahr1a, ahr1b, ahr2) and nrf2 (nrf2a, nrf2b), of which ahr2 has been reported to mediate the effects of classic AHR ligands such as
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TCDD and PCB126 (Andreasen et al., 2002; Liu et al., 2016). In this study, we aimed
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to investigate the effects of AHR-induced oxidative stress on the cardiac developmental toxicity of TCE in zebrafish embryos. ROS and DNA damage levels,
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as well as the gene expression/activity of AHR and Nrf2 signalling genes were also examined in the heart of zebrafish embryos under different treatment conditions. The
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role of AHR was then confirmed by using Morpholino (MO)-mediated AHR
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knockdown.
2. Materials and methods 2.1 Chemicals
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Trichloroethylene (TCE, CAS 79-01-6), Trichloroacetic acid (TCA, CAS 76-03-9), Benzo [a] pyrene（BaP, CAS 50-32-8) and N-Acetyl-L-cysteine (NAC, CAS 616-91-1) were purchased from Adamas-Beta (Shanghai, China). CH223191 (CH, CAS 301326-22-7) and StemRegenin 1 (SR1, CAS 1227633-49-9) were obtained from MedChemExpress (Shanghai, China) and TargetMol (Shanghai, China), respectively. 2,4,3′,5′-tetramethoxystilbene (TMS, CAS 24144-92-1) was obtained from
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Topscience (Shanghai, China). All other chemicals were from Sigma-Aldrich (Shanghai, China) unless otherwise stated.
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2.2 Fish maintenance and embryo treatment
All experiments were conducted in accordance with the Institutional Animal
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Care/User Ethical Committee of Soochow University, Suzhou City, China. Mature
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wild-type adult zebrafish (AB-strain) were obtained from China Zebrafish Resource Centre (Wuhan, China) and maintained at 28.5 ± 0.5℃ in a zebrafish facility
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containing buffered water with a photoperiod of 14-hour light and 10-hour dark cycles. Sexually mature fish without any deformities or signs of disease were selected as
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breeders. Fertilized eggs were visually selected and only normally developed eggs
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were used for further testing. Eggs were then exposed to chemicals from 3-hour post-fertilization (hpf) in glass petri dishes with DMSO as a vehicle control. The solutions were renewed daily with fresh medium. Embryos at 72 hpf were transferred to concave microscope slides for the detection of cardiac abnormalities under a stereo
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microscope, and at least 100 embryos were examined for each group. The hearts were dissected and collected as described previously (Yue et al., 2017). 2.3 Quantitative Real-Time PCR (qPCR) Total RNA was extracted from isolated hearts (n=50) using TRNzol A+ reagent (Tiangen, Beijing, China), and 1 μg was used for cDNA synthesis by RevertAidTM First Strand cDNA Synthesis Kit (Thermo Scientific Fermentas, USA). qPCR was
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carried out using FS Universal SYBR Green Master mix (Roche, shanghai, China) in an ABI QuantStudio 6 qPCR system (Applied Biosystems). Primer sequences were
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listed in Table S1. The Ct values of target genes were normalized to those of gapdh and elfa, and the relative expression change was calculated using the comparative
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2.4 Antioxidant activity assays
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threshold cycle method (2-ΔΔCt) as described before (Livak and Schmittgen, 2001).
Whole embryo catalase (Cat) activity was measured by using a Catalase activity
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assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer’s instructions. The activities of total Sod, Sod1 and Sod2 in whole
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embryos were measured by using a Superoxide Dismutase Typed Assay Kit
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(Nanjing Jiancheng Bioengineering Institute, China). 2.5 In vivo Ethoxyresorufin-O-deethylase (EROD) assay Cyp1a1 activity was measured by a modified in vivo EROD assay (Le Bihanic et al., 2013). Briefly, embryos from each group were incubated in fish water with 0.4 μg/mL 7-ethoxyresorufin for 30 min. After washing for 3 mins, the embryos were 8
anesthetized by MS-222 and imaged by using a fluorescent microscope (Olympus IX73, Japan). The fluorescence intensity was quantified by using image J. At least 20 embryos were examined for each group. 2.6 ROS measurement The production of ROS was measured using dichlorodihydrofluorescein diacetate (DCFH-DA). Briefly, zebrafish (n=10 per group) were incubated with 20 μM
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DCFH-DA in the dark for 30 mins and then washed three times. Fluorescent
microscopic images were taken by using an Olympus IX73 microscope. At least 30
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embryos were examined for each group. 2.7 Oil-Red-O staining
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Oil-Red-O (ORO) staining was used to detect lipid distribution. Embryos were fixed
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in 4% paraformaldehyde (PFA) at 4°C overnight and then washed with 60% isopropanol for one hour. After incubating in 1 ml of ORO working solution (0.25%
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ORO stock in 60% isopropanol) for 1 hour, the embryos were washed twice with 60% isopropanol and photographed under a Nikon SMZ1500 zoom stereomicroscope
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group.
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(Nikon Corporation, Tokyo, Japan). At least 20 embryos were examined for each
2.8 Acridine Orange staining To detect cell apoptosis in the heart region, live embryos were stained with acridine orange (AO). Specifically, zebrafish embryos were washed with egg water three times and incubated with 5 μg/mL AO for 30 mins in the dark. The embryos were then 9
thoroughly washed with egg water again before being imaged under a fluorescent microscope (Olympus IX73, Olympus Corporation, Tokyo, Japan). At least 20 embryos were examined for each group. 2.9 Immunofluorescence Hearts dissected from embryos at 72 hpf were fixed with 4% PFA and permeabilized with 2% TritonX-100 before being blocked with 5% goat serum. After probing with
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primary antibodies against AHR (Immunoway Biotechnology, Plano, TX, USA) and
8-OHdG (sc-66036, Santa Cruz, EU) overnight at 4°C, the embryos were washed and
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incubated with secondary antibody labelled with Alexa Fluor 555 and Alexa Fluor
488 respectively (Invitrogen, Carlsbad, CA, USA). The distribution of fluorescence
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intensity was observed under a fluorescent microscopy. At least 15 embryos were
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examined for each group. 2.10 Morpholino Injection
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Morpholino (MO) antisense oligonucleotides were designed and produced by Gene Tools, LLC (Philomath, OR, USA). AHR knockdown was achieved by using the
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designed ahr2 MO (5'-TGTACCGATACCCGCCGACATGGTT-3') and Gene Tools’
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standard control MO (5'-CCTCTTACCTCAGTTACAATTTATA-3'). The MOs were re-suspended in RNase free water as a 3mM stock solution and diluted to 0.5 mM as the working solution for microinjections. Ahr2 MO and control MO were injected into zebrafish embryos at one-two cell stage with the micro-injector (Applied Scientific Instrumentation MPPI-3, Eugene, USA). 10
2.11 Statistical Analysis All experiments were performed at least three times, and results were represented as mean ± SEM. The statistical methods used were one-way ANOVA followed by Dunnett's or Turkey’s post-hoc test when appropriate. A P value of <0.05 was considered as statistically significant difference between groups.
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3. Results 3.1 Heart defects of zebrafish embryos
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As shown in Figure 1, TCE at 1 ppb (7.6 nM) had little effect on zebrafish heart development, but TCE at 10 ppb (76 nM) and 100 ppb (760 nM) significantly
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increased heart malformations such as balloon- shaped heart chambers and severe
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pericardial edema. TCA at 10 ppb (6 nM) also significantly increased heart defects, but to a lesser extent than that caused by TCE at the same concentration.
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Supplementation with AHR inhibitors, CH and SR1 significantly attenuated the heart defects in embryos exposed to TCE at 10 ppb, with the heart malformation rates being
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reduced to control levels by CH. It is worth noting that the ROS scavenger, NAC, also
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counteracted the EOM-induced cardiac malformations. Fish survival rate was only decreased by TCE at the highest dose level (100 ppb). 3.2 Expression and activity of AHR signaling genes We first tested the effect of TCE on the mRNA expression of genes involved in AHR signaling pathway including ahr2, ahrra, ahrrb, cyp1a1 and cyp1b1. As shown in 11
Figure 2A, only cyp1b1 was significantly upregulated by TCE, which was counteracted by AHR inhibitor, CH. We further found that TMS, a selective cyp1b1 inhibitor, partly attenuated the TCE-induced heart defects, with the malformation rates being dropped from 15.48 % to 10.06 % (Figure 2A). In the heart of zebrafish embryos, TCE had no detectable effects on either AHR protein level or EROD activity which mainly measures the activity of Cyp1a1 (Figure 2B and 2C). In
expression levels and EROD activity (Figures 2B and 2C).
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3.3 Oxidative stress
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contrast, the typical AHR agonist, BaP induced a significant increase in AHR
Treatment with TCE significantly increased ROS and 8-OHdG levels in the heart of
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zebrafish embryos, albeit to a lesser extent when compared to those induced by BaP
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(Figure 3). Both AHR inhibitor, CH and ROS scavenger, NAC, counteracted the effect of TCE on ROS and 8-OHdG (Figure 3). As shown in Figure 4, TCE had no
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effect on lipid consumption (ORO staining) or apoptosis (AO staining) in the heart of zebrafish embryos, while BaP significantly increased ORO and AO staining levels,
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indicating promotion of apoptosis and impaired lipid consumption.
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For the oxidative stress-related genes including cat, sod1, sod2, ho1, nqo1, gstp1, gstp2, nrf2a, nrf2b and keap1a, TCE downregulated nrf2b and upregulated sod2, ho1 and nqo1 (Figure 5A). In samples exposed to TCE plus CH, gstp2, sod2, ho1, nqo1 returned to control gene expression levels while nrf2b was even overexpressed (Figure 5A). TCE had no effect on Cat, but the activities of total Sod, Sod1 and Sod2 12
were all increased in embryos exposed to TCE and then attenuated by CH supplementation (Figure 5B). 3.4 Expression of genes essential to heart development We examined the effect of TCE on genes essential to cardiac differentiation. As demonstrated in Figure 6, TCE decreased the mRNA levels of gata4 and hand2 increased those of c-fos and sox9b, which were significantly attenuated in the heart of
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embryos exposed to TCE plus CH. However, TCE had no effect on the expression levels of nkx2.5 and axin2 (Figure 6).
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3.5 Effects of AHR knockdown
As expected, AHR knockdown by MO significantly attenuated TCE-induced heart
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defects (Figure S1). Although EROD activity was not affected by TCE treatment, its
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background level was significantly reduced by AHR knockdown (Figure 7A). Similar effects were observed for the expression levels of AHR protein and Cyp1a1 mRNA
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(Figure S2). As shown in Figure 7B and 7C, AHR knockdown with morpholino
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significantly attenuated the TCE-induced upregulation of ROS and 8-OHdG levels.
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4. Discussion
Increasing evidence indicates that TCE has the potential to cause cardiac malformations, but the underlining mechanism(s) remain unclear (Caldwell et al., 2008; Drake et al., 2006; Makris et al., 2016; Rufer et al., 2010; Wirbisky et al., 2016). In early development, oxidative stress can trigger the signal pathways 13
involved in cellular functions such as cell proliferation, differentiation and apoptosis (Asoglu et al., 2018). In addition, elevated ROS has been found to alter the expression of genes crucial to the differentiation of cardiac neural crest cells, leading to heart defects during embryonic development (Asoglu et al., 2018; Dennery, 2007). In this study, we found that TCE elevated ROS levels and 8-OHdG signals (a well-known marker of oxidative stress and DNA damage) in the heart of zebrafish
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embryos. In addition, the TCE-induced heart defects were significantly counteracted
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by the ROS scavenger, NAC.
Oxidative stress has also been known to induce lipid peroxidation leading to
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disordered lipid distribution (Hauck and Bernlohr, 2016; Huang et al., 2018).
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However, changes in lipid distribution were only detected in the heart of embryos exposed to BaP but not to TCE. Considering that the ROS level induced by TCE was
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much lower than that by BaP in our study, we hypothesize that the TCE-induced
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oxidative stress may not have been robust enough to affect lipid metabolism.
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Nrf2 is the main regulator of the adaptive response to oxidative stress. Elevated ROS levels lead to the oxidation of Keap1 and the release of Nrf2, which then translocates to the nucleus and activates the transcription of various antioxidant and phase II detoxification genes. Zebrafish has two nrf2 genes, nrf2a and nrf2b, with the later showing negative regulatory effects during development (Rousseau et al., 2015). In 14
our study, TCE had no effect on nrf2a mRNA expression, but the mRNA expression level of nrf2b was significantly decreased in the heart of zebrafish embryos exposed to TCE. Consistent with these observations, ho1, the direct target genes of nrf2b, was upregulated by TCE. In addition, the mRNA expression levels of nrf2a downstream genes, including sod2, gstp2 and nqo1, were also upregulated, with increased enzyme activities of total Sod, Sod1 and Sod2, indicating that Nrf2a was activated
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through a post-transcriptional mechanism in the heart of zebrafish embryos exposed to TCE. Ho1, Nqo1, Sod1/2 and Gstp2 all have antioxidant effects, but
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overexpression of these antioxidant enzymes and high levels of ROS are frequent findings in TCE-exposed samples (Biswas et al., 2014; Klotz and Steinbrenner,
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2017). One possible explanation is that the overexpression changes of the four genes
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TCE-induced ROS.
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are only 1.5-2.5 times in magnitude, which may not be enough to scavenge
Because we found that both AHR inhibitors CH and SR1 protected zebrafish
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embryos from the cardiac developmental toxicity of TCE, we rationalized that AHR
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might play an essential role in the TCE-induced heart defects. Our MO knockdown experiments further confirmed the role of AHR in the cardiac developmental toxicity of TCE. Moreover, the TCE-induced ROS and 8-OHdG signals were abolished by AHR inhibition via either supplementation of CH or by gene knockdown. CH also attenuated the TCE-induced mRNA expression changes of genes essential to heart 15
development including gata4, hand2, c-fos and sox9b. It should be noted that we only used ahr2 MO to knockdown AHR, indicating that ahr2 is responsible for the toxic effects of TCE. However, we cannot rule out the possibility that ahr1a/1b can also play a role in the cardiac developmental toxicity of TCE.
Given the known structural requirements for binding and activating DREs generated
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by Casiewicz et al, TCE is not expected to activate canonical AHR signaling
(Gasiewicz et al., 1996). Consistent with this, TCE cannot active DRE in a human
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cell-based luciferase reporter gene assay from the Tox21 database (NCBI, 2014). However, recent studies also revealed non-canonical AHR signaling pathways,
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during which AHR interacts with different ligands and modulates transcription
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through sites distinct from DRE (Wright et al., 2017). AHR can also bind to, and be activated by a variety of chemicals with structures dramatically different from the
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classic AHR ligands such as PAHs (polyaromatic aromatic hydrocarbons) and HAHs (halogenated aromatic hydrocarbons), and a number of endogenous AHR ligands
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have been identified (Kawajiri and Fujii-Kuriyama, 2017). Ethanol, which does not
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meet the structural requirements just like TCE, has been reported to activate AHR in mouse hepatic stellate cells (Zhang et al., 2012). In addition, ultraviolet B (UVB) can activate AHR by generating the tryptophan photoproduct, 6-Formylindolo [3,2-b] carbazole (FICZ), which serves as an endogenous AHR agonist (Furue et al., 2019). Thus, we hypothesize that TCE might activate AHR by generating endogenous 16
ligands in the heart of zebrafish embryos.
It has been suggested that AHR can induce oxidative stress by upregulating Cyp1a1 (Livak and Schmittgen, 2001), yet neither the cyp1a1 mRNA expression nor its activity as measured by the EROD assay was upregulated by TCE in this study. Nevertheless, cyp1b1 was overexpressed in the heart of zebrafish embryos exposed to
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TCE. It has been reported that Cyp1b1 plays a direct role in PAH-mediated oxidative damage (Green et al., 2008). Cyp1b1 can induce ROS generation by bioactivating
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xenobiotics and activating NADPH oxidase (Green et al., 2008; Yaghini et al., 2010). Thus, Cyp1b1 instead of Cyp1a1 might mediate the TCE-induced oxidative stress in
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the heart of zebrafish embryos, which is consistent with our observations that the
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selective Cyp1b1 inhibitor, TMS, partly attenuated the TCE-induced heart defects.
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Cyp1a1 is a classical AHR target gene, but recent studies suggest that different AHR ligands can induce distinct ligand specific sets of AHR-dependent genes (Denison and
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Faber, 2017). Activation of AHR can even form heterodimers with nuclear proteins
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other than ARNT to regulate the expression of genes lacking DRE (Denison and Faber, 2017). It will be interesting to find out if other small organic chemicals which do not possess the classic structure of typical AHR agonists can also activate AHR dependent genes in a similar manner as TCE.
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A crosstalk between AHR and Nrf2 has been well documented (Haarmann-Stemmann et al., 2012; Kohle and Bock, 2007; Yeager et al., 2009; Zgheib et al., 2018). The AHR agonist, TCDD, can increase Nrf2 protein level and activate Nrf2 activity in mice (Wang et al., 2013a). The presence of multiple potential DREs in the promoter and first intron of nrf2a and nrf2b indicates that their expression levels might be regulated by AHR (Rousseau et al., 2015). Timme et al
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found that Nrf2b instead of Nrf2a was induced by TCDD in an AHR dependent
manner in zebrafish embryos, prompting a higher sensitivity of Nrf2b to AHR than
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that of Nrf2a (Timme-Laragy et al., 2012). In this study, we found that AHR
inhibitor, CH, counteracted the TCE-induced downregulation of Nrf2b. The mRNA
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expression level of ho1, a direct Nrf2b target gene, was also upregulated by TCE and
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restored by TCE plus CH. It is worth noting that in our study, Nrf2b is repressed rather than activated by AHR, which is contrary to a previous report (Timme-Laragy
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et al., 2012). This may be due to differences in inducers, doses, exposure duration or some other factors. Although TCE had no effect on nrf2a mRNA expression, TCE
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upregulated the mRNA expression levels and activities of Nrf2a downstream genes,
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which were attenuated by CH supplementation, suggesting that AHR mediates the activation of Nrf2a induced by TCE at a post-transcriptional level. Future studies would be needed to quantify the effect of TCE on Nrf2a protein levels.
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TCE is metabolized in vivo via two major pathways: cytochrome P450 oxidation pathway and glutathione (GSH) synthesis pathway. TCA, the main oxidative pathway metabolite of TCE, has been reported to increase the incidence of cardiac defects (Johnson et al., 1998a, b; Smith et al., 1989). However, in our study, the TCA-induced heart defects were of much lower severity than the equivalent defects caused by TCE. Instead, we found that the mRNA expression level of glutathione transferase gstp2
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was increased, indicating that GSH synthesis pathway might play a crucial role in the cardiac developmental toxicity of TCE. Future studies are warranted to examine the
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the cardiac development of zebrafish embryos.
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effects of GSH synthesis pathways and the metabolites of TCE (such as DCVC) on
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5. Conclusions
Our study indicates that in the heart of zebrafish embryos, TCE can activate AHR,
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which could enhance ROS generation via Cyp1b1 overexpression, leading to cardiac defects. Most significantly, AHR activation is a necessary step for the ROS-induced
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Nrf2-antioxidant response (See Figure 8). To the best of our knowledge, this is the
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first study showing that AHR plays an important role in the cardiac developmental toxicity of TCE. Given the abundance of natural AHR antagonists and antioxidants in food, our results provide the scientific basis for the selection of candidate entities for possible development as agents for use in the prevention or treatment of TCE-induced CHDs. 19
Declarations of interest: None Conflict of interest statement All authors declare no conflict of interest.
Acknowledgments This work was supported by the National Nature Sciences Foundation of China (Grant
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number: 81570284), Applied Basic Research in Suzhou (Grant number: sys201519) and The Priority Academic Program Development of Jiangsu Higher Education
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Institutions. We thank Prof. Han Wang for his help in preparing zebrafish embryos.
References
Agency for Toxic Substances and Disease Registry (ATSDR). 2017. ATSDR’s Substance Priority List.
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https://www.atsdr.cdc.gov/spl/index.html [accessed 9 May 2019]
Andreasen, E.A., Spitsbergen, J.M., Tanguay, R.L., Stegeman, J.J., Heideman, W., Peterson, R.E., 2002. Tissue-specific expression of AHR2, ARNT2, and CYP1A in zebrafish embryos and larvae: effects of
na
developmental stage and 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure. Toxicol Sci 68, 403-419. Asoglu, M.R., Gabbay-Benziv, R., Turan, O.M., Turan, S., 2018. Exposure of the developing heart to diabetic environment and early cardiac assessment: A review. Echocardiography 35, 244-257. Biswas, C., Shah, N., Muthu, M., La, P., Fernando, A.P., Sengupta, S., Yang, G., Dennery, P.A., 2014.
ur
Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses. J Biol Chem 289, 26882-26894. Blossom, S.J., Doss, J.C., Hennings, L.J., Jernigan, S., Melnyk, S., James, S.J., 2008. Developmental
Jo
exposure to trichloroethylene promotes CD4+ T cell differentiation and hyperactivity in association with oxidative stress and neurobehavioral deficits in MRL+/+ mice. Toxicol Appl Pharmacol 231, 344-353.
Blossom, S.J., Melnyk, S.B., Li, M., Wessinger, W.D., Cooney, C.A., 2017. Inflammatory and oxidative stress-related effects associated with neurotoxicity are maintained after exclusively prenatal trichloroethylene exposure. Neurotoxicology 59, 164-174. Brown, D.R., Samsa, L.A., Qian, L., Liu, J., 2016. Advances in the Study of Heart Development and Disease Using Zebrafish. J Cardiovasc Dev Dis 3.
20
Caldwell, P.T., Thorne, P.A., Johnson, P.D., Boitano, S., Runyan, R.B., Selmin, O., 2008. Trichloroethylene disrupts cardiac gene expression and calcium homeostasis in rat myocytes. Toxicol Sci 104, 135-143. Carreira, V.S., Fan, Y., Kurita, H., Wang, Q., Ko, C.I., Naticchioni, M., Jiang, M., Koch, S., Zhang, X., Biesiada, J., Medvedovic, M., Xia, Y., Rubinstein, J., Puga, A., 2015. Disruption of Ah Receptor Signaling during Mouse Development Leads to Abnormal Cardiac Structure and Function in the Adult. PLoS One 10, e0142440. Chiba, T., Uchi, H., Tsuji, G., Gondo, H., Moroi, Y., Furue, M., 2011. Arylhydrocarbon receptor (AhR) activation in airway epithelial cells induces MUC5AC via reactive oxygen species (ROS) production. Pulmonary Pharmacology & Therapeutics 24, 133-140. Denison, M.S., Faber, S.C., 2017. And Now for Something Completely Different: Diversity in Ligand-Dependent Activation of Ah Receptor Responses. Curr Opin Toxicol 2, 124-131.
ro of
Dennery, P.A., 2007. Effects of oxidative stress on embryonic development. Birth Defects Res C Embryo Today 81, 155-162.
Drake, V.J., Koprowski, S.L., Hu, N., Smith, S.M., Lough, J., 2006. Cardiogenic effects of
trichloroethylene and trichloroacetic acid following exposure during heart specification of avian development. Toxicol Sci 94, 153-162.
-p
United States Environmental Protection Agency (US EPA). Toxicological Review of Trichloroethylene. Available: https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=215006#tab-3 [accessed 9 May 2019].
re
Furue, M., Uchi, H., Mitoma, C., Hashimoto-Hachiya, A., Tanaka, Y., Ito, T., Tsuji, G., 2019. Implications of tryptophan photoproduct FICZ in oxidative stress and terminal differentiation of keratinocytes. G Ital Dermatol Venereol 154, 37-41.
lP
Gasiewicz, T.A., Kende, A.S., Rucci, G., Whitney, B., Willey, J.J., 1996. Analysis of structural requirements for Ah receptor antagonist activity: ellipticines, flavones, and related compounds. Biochem Pharmacol 52, 1787-1803.
Green, R.M., Hodges, N.J., Chipman, J.K., O'Donovan, M.R., Graham, M., 2008. Reactive oxygen
na
species from the uncoupling of human cytochrome P450 1B1 may contribute to the carcinogenicity of dioxin-like polychlorinated biphenyls. Mutagenesis 23, 457-463. Haarmann-Stemmann, T., Abel, J., Fritsche, E., Krutmann, J., 2012. The AhR-Nrf2 pathway in
ur
keratinocytes: on the road to chemoprevention? J Invest Dermatol 132, 7-9. Harmon, A.C., Hebert, V.Y., Cormier, S.A., Subramanian, B., Reed, J.R., Backes, W.L., Dugas, T.R., 2018. Particulate matter containing environmentally persistent free radicals induces AhR-dependent
Jo
cytokine and reactive oxygen species production in human bronchial epithelial cells. PLoS One 13, e0205412.
Hauck, A.K., Bernlohr, D.A., 2016. Oxidative stress and lipotoxicity. J Lipid Res 57, 1976-1986. Huang, M., Jiao, J., Wang, J., Xia, Z., Zhang, Y., 2018. Characterization of acrylamide-induced oxidative stress and cardiovascular toxicity in zebrafish embryos. J Hazard Mater 347, 451-460. Jiang, Y., Wang, D., Zhang, G., Wang, G., Tong, J., Chen, T., 2016. Disruption of cardiogenesis in human embryonic stem cells exposed to trichloroethylene. Environ Toxicol 31, 1372-1380. Johnson, P.D., Dawson, B.V., Goldberg, S.J., 1998a. Cardiac teratogenicity of trichloroethylene metabolites. J Am Coll Cardiol 32, 540-545. 21
Johnson, P.D., Dawson, B.V., Goldberg, S.J., 1998b. A review: trichloroethylene metabolites: potential cardiac teratogens. Environ Health Perspect 106 Suppl 4, 995-999. Jonsson, M.E., Jenny, M.J., Woodin, B.R., Hahn, M.E., Stegeman, J.J., 2007. Role of AHR2 in the expression of novel cytochrome P450 1 family genes, cell cycle genes, and morphological defects in developing zebra fish exposed to 3,3',4,4',5-pentachlorobiphenyl or 2,3,7,8-tetrachlorodibenzo-p-dioxin. Toxicol Sci 100, 180-193. Kawajiri, K., Fujii-Kuriyama, Y., 2017. The aryl hydrocarbon receptor: a multifunctional chemical sensor for host defense and homeostatic maintenance. Exp Anim 66, 75-89. Klotz, L.O., Steinbrenner, H., 2017. Cellular adaptation to xenobiotics: Interplay between xenosensors, reactive oxygen species and FOXO transcription factors. Redox Biol 13, 646-654. Kohle, C., Bock, K.W., 2007. Coordinate regulation of Phase I and II xenobiotic metabolisms by the Ah receptor and Nrf2. Biochem Pharmacol 73, 1853-1862.
ro of
Lanham, K.A., Plavicki, J., Peterson, R.E., Heideman, W., 2014. Cardiac myocyte-specific AHR activation phenocopies TCDD-induced toxicity in zebrafish. Toxicol Sci 141, 141-154.
Lash, L.H., Fisher, J.W., Lipscomb, J.C., Parker, J.C., 2000. Metabolism of trichloroethylene. Environ Health Perspect 108 Suppl 2, 177-200.
Le Bihanic, F., Couillard, C.M., Rigaud, C., Legare, B., 2013. A simple and reliable in vivo EROD activity
-p
measurement in single Fundulus heteroclitus embryo and larva. Mar Environ Res 84, 17-23.
Liu, H., Nie, F.H., Lin, H.Y., Ma, Y., Ju, X.H., Chen, J.J., Gooneratne, R., 2016. Developmental toxicity, EROD, and CYP1A mRNA expression in zebrafish embryos exposed to dioxin-like PCB126. Environ
re
Toxicol 31, 201-210.
Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402-408.
lP
Ma, Q., 2013. Role of nrf2 in oxidative stress and toxicity. Annu Rev Pharmacol Toxicol 53, 401-426. Ma, Q., Kinneer, K., Bi, Y., Chan, J.Y., Kan, Y.W., 2004. Induction of murine NAD(P)H:quinone oxidoreductase by 2,3,7,8-tetrachlorodibenzo-p-dioxin requires the CNC (cap 'n' collar) basic leucine zipper transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2): cross-interaction
na
between AhR (aryl hydrocarbon receptor) and Nrf2 signal transduction. Biochem J 377, 205-213. Makris, S.L., Scott, C.S., Fox, J., Knudsen, T.B., Hotchkiss, A.K., Arzuaga, X., Euling, S.Y., Powers, C.M., Jinot, J., Hogan, K.A., Abbott, B.D., Hunter, E.S., 3rd, Narotsky, M.G., 2016. A systematic evaluation of
ur
the potential effects of trichloroethylene exposure on cardiac development. Reprod Toxicol 65, 321-358.
NCBI, 2014. NCBI National Center for Biotechnology Information . PubChem BioAssay Database;
Jo
AID=743122, .
Rooney, J.P., Oshida, K., Kumar, R., Baldwin, W.S., Corton, J.C., 2018. Chemical Activation of the Constitutive Androstane Receptor (CAR) Leads to Activation of Oxidant-Induced Nrf2. Toxicol Sci. Rousseau, M.E., Sant, K.E., Borden, L.R., Franks, D.G., Hahn, M.E., Timme-Laragy, A.R., 2015. Regulation of Ahr signaling by Nrf2 during development: Effects of Nrf2a deficiency on PCB126 embryotoxicity in zebrafish (Danio rerio). Aquat Toxicol 167, 157-171. Rufer, E.S., Hacker, T.A., Flentke, G.R., Drake, V.J., Brody, M.J., Lough, J., Smith, S.M., 2010. Altered cardiac function and ventricular septal defect in avian embryos exposed to low-dose trichloroethylene. Toxicol Sci 113, 444-452. 22
Sarmah, S., Marrs, J.A., 2016. Zebrafish as a Vertebrate Model System to Evaluate Effects of Environmental Toxicants on Cardiac Development and Function. Int J Mol Sci 17. Smith, M.K., Randall, J.L., Read, E.J., Stober, J.A., 1989. Teratogenic activity of trichloroacetic acid in the rat. Teratology 40, 445-451. Takei, K., Hashimoto-Hachiya, A., Takahara, M., Tsuji, G., Nakahara, T., Furue, M., 2015. Cynaropicrin attenuates UVB-induced oxidative stress via the AhR-Nrf2-Nqo1 pathway. Toxicol Lett 234, 74-80. Teraoka, H., Dong, W., Tsujimoto, Y., Iwasa, H., Endoh, D., Ueno, N., Stegeman, J.J., Peterson, R.E., Hiraga, T., 2003. Induction of cytochrome P450 1A is required for circulation failure and edema by 2,3,7,8-tetrachlorodibenzo-p-dioxin in zebrafish. Biochem Biophys Res Commun 304, 223-228. Timme-Laragy, A.R., Karchner, S.I., Franks, D.G., Jenny, M.J., Harbeitner, R.C., Goldstone, J.V., McArthur, A.G., Hahn, M.E., 2012. Nrf2b, novel zebrafish paralog of oxidant-responsive transcription factor NF-E2-related factor 2 (NRF2). J Biol Chem 287, 4609-4627.
ro of
Toraason, M., Clark, J., Dankovic, D., Mathias, P., Skaggs, S., Walker, C., Werren, D., 1999. Oxidative stress and DNA damage in Fischer rats following acute exposure to trichloroethylene or perchloroethylene. Toxicology 138, 43-53.
Wang, L., He, X., Szklarz, G.D., Bi, Y., Rojanasakul, Y., Ma, Q., 2013a. The aryl hydrocarbon receptor interacts with nuclear factor erythroid 2-related factor 2 to mediate induction of
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NAD(P)H:quinoneoxidoreductase 1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Arch Biochem Biophys 537, 31-38.
Wang, Q., Chen, J., Ko, C.I., Fan, Y., Carreira, V., Chen, Y., Xia, Y., Medvedovic, M., Puga, A., 2013b.
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Disruption of aryl hydrocarbon receptor homeostatic levels during embryonic stem cell differentiation alters expression of homeobox transcription factors that control cardiomyogenesis. Environ Health Perspect 121, 1334-1343.
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Wei, H., Cong, X., 2018. The effect of reactive oxygen species on cardiomyocyte differentiation of pluripotent stem cells. Free Radic Res 52, 150-158.
Wincent, E., Stegeman, J.J., Jonsson, M.E., 2015. Combination effects of AHR agonists and Wnt/beta-catenin modulators in zebrafish embryos: Implications for physiological and toxicological
na
AHR functions. Toxicol Appl Pharmacol 284, 163-179.
Wirbisky, S.E., Damayanti, N.P., Mahapatra, C.T., Sepulveda, M.S., Irudayaraj, J., Freeman, J.L., 2016. Mitochondrial Dysfunction, Disruption of F-Actin Polymerization, and Transcriptomic Alterations in
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Zebrafish Larvae Exposed to Trichloroethylene. Chem Res Toxicol 29, 169-179. Wright, E.J., Pereira De Castro, K., Joshi, A.D., Elferink, C.J., 2017. Canonical and non-canonical aryl hydrocarbon receptor signaling pathways. Current Opinion in Toxicology 2, 87-92.
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Yaghini, F.A., Song, C.Y., Lavrentyev, E.N., Ghafoor, H.U., Fang, X.R., Estes, A.M., Campbell, W.B., Malik, K.U., 2010. Angiotensin II-induced vascular smooth muscle cell migration and growth are mediated by cytochrome P450 1B1-dependent superoxide generation. Hypertension 55, 1461-1467. Yeager, R.L., Reisman, S.A., Aleksunes, L.M., Klaassen, C.D., 2009. Introducing the "TCDD-inducible AhR-Nrf2 gene battery". Toxicol Sci 111, 238-246. Yue, C., Ji, C., Zhang, H., Zhang, L.W., Tong, J., Jiang, Y., Chen, T., 2017. Protective effects of folic acid on PM2.5-induced cardiac developmental toxicity in zebrafish embryos by targeting AhR and Wnt/beta-catenin signal pathways. Environ Toxicol 32, 2316-2322.
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Zgheib, E., Limonciel, A., Jiang, X., Wilmes, A., Wink, S., van de Water, B., Kopp-Schneider, A., Bois, F.Y., Jennings, P., 2018. Investigation of Nrf2, AhR and ATF4 Activation in Toxicogenomic Databases. Front Genet 9, 429. Zhang, H.F., Lin, X.H., Yang, H., Zhou, L.C., Guo, Y.L., Barnett, J.V., Guo, Z.M., 2012. Regulation of the activity and expression of aryl hydrocarbon receptor by ethanol in mouse hepatic stellate cells. Alcohol Clin Exp Res 36, 1873-1881. Zhou, B., Wang, X., Li, F., Wang, Y., Yang, L., Zhen, X., Tan, W., 2017. Mitochondrial activity and oxidative stress functions are influenced by the activation of AhR-induced CYP1A1 overexpression in
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cardiomyocytes. Mol Med Rep 16, 174-180.
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Figure Legends: Figure 1. Cardiac defects and survival rate of zebrafish embryos at 72 hpf. A) Represent images of zebrafish embryos at 72 hpf. Dotted lines encircle atrium (red) or ventricle (blue). TCE1, 10, 100: Trichloroethylene at 1 ppb, 10 ppb, 100 ppb; TCA10: Trichloroacetic acid at 10 ppb; BaP: Benzo[a]pyrene at 0.1 μM; CH: AHR inhibitor, CH223191 at 0.125 μM; SR1: AHR inhibitor, StemRegenin 1 at 0.25 μM; NAC: ROS
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scavenger, N-Acetyl-L-cysteine at 0.25 μM. B) Heart malformation rates. C) Survival rates. Different letters (a-c) indicate significant differences.
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Figure 2. Expression and activity of AHR signaling genes in the heart of
zebrafish embryos at 72 hpf. A) mRNA expression and heart malformation rates.
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TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at 0.125 μM; B)
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Immunostaining of AHR and the relevant quantification results. BaP: Benzo[a]pyrene at 0.1 μM; NAC: ROS scavenger, N-Acetyl-L-cysteine at 0.25 μM. Different letters
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(a-b) indicate significant differences. C) Representative images and quantification results of Ethoxyresorufin-O-deethylase (EROD) activity (a catalytic measurement of
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Cyp1a1 induction). The dotted areas indicate heart regions; ***, p<0.001.
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Figure 3. ROS and 8-OHdG signals in the heart of zebrafish embryos at 72 hpf. A) ROS signal and the quantification results. The dotted areas indicate heart regions. B) Immunostaining of 8-OhdG and the relevant quantification results. TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at 0.125 μM; NAC: ROS
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scavenger, N-Acetyl-L-cysteine at 0.25 μM; BaP: Benzo[a]pyrene at 0.1 μM. Different letters (a-c) indicate significant differences. Figure 4. Lipid distribution and apoptosis in the heart of zebrafish embryos at 72 hpf. A) Representative images of lipid distribution stained by Oil-Red-O and the quantification result. B) Representative images of apoptosis stained by acridine orange and the quantification result. i) Whole fish; ii) Enlarged pictures showing the
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heart region. Dotted lines encircle atrium (red) or ventricle (blue). TCE10,
Trichloroethylene at 10 ppb; BaP: Benzo[a]pyrene at 0.1 μM. ***, p<0.001.
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Figure 5. Oxidative stress related gene expression/activity levels in the heart of
zebrafish embryos at 72 hpf. A) mRNA expression changes. B) Catalase and SOD
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activities. TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at
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0.125 μM; Different letters (a-c) indicate significant differences. Figure 6. mRNA expression changes of genes essential to heart development in
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zebrafish embryos at 72 hpf. TCE10, Trichloroethylene at 10 ppb; CH: AHR inhibitor, CH223191 at 0.125 μM; Different letters (a-c) indicate significant
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differences.
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Figure 7. EROD activity, ROS level and AHR/8-OHdG levels in AHR knockdown zebrafish embryos at 72 hpf (n≥5). A) Ethoxyresorufin-O-deethylase (EROD) activity. B) Reactive oxygen species (ROS) signal. C) Immunostaining of 8-OHdG and the quantification results. The dotted areas indicate heart regions.
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TCE10, Trichloroethylene at 10 ppb; ConMO: non-specific control morpholino; AHRMO: ahr2 morpholino. Different letters (a-c) indicate significant differences. Figure 8. Model showing that AHR-mediated oxidative stress contributes to TCE cardiac developmental toxicity. TCE-activated aryl hydrocarbon receptor (AHR) increases reactive oxygen species (ROS) via cyp1b1 overexpression, leading to heart malformations. ROS in turn activates Nrf2 (Nrf2a/2b) antioxidant signal
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pathway, and AHR activity is required for the regulation of Nrf2 target genes.
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Inhibition of AHR or ROS counteracts TCE-induced heart defects.
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