Alcohol: Novel antimicrobial technology for persistent activity

AJIC

Volume 27, Number 2

Abstracts 2 0 9

STUDY 2 (Single/Repeated Application)~

STUDY 1 (Single Application)~

Skin Type Dry Normal

5-10 mins

15-20 mins

30-35 rains

45-50 mins

60-65 rains

5 mins (Single)

5 mins (Repeated)

139.9 1" 190.2 1-

34.7 1 48.1 1-

18.6 125.7 1-

11.8 118.8 1"

16,1 110.4

39.1 t 43.4 1"

21,1 125,8 1"

1- - Statistically significant (p>0.05) moisturization ¢ - Compared to baseline

METHODS: Two independent clinical studies were undertaken to measure the moisturization properties of a 60% ethyl alcohol hand gel (PREVACARET M Antimicrobial Hand Gel). In Study 1, randomized test sites on the lower legs of 40 subjects with mild-to-moderate, dry skin or normal, non-dry skin were evaluated over time to quantify the relative hydration of the stratum corneum prior to treatment and following a single application of test material in comparison to an untreated leg. In Study 2, randomized test sites on the lower legs of 43 subjects with dry or normal skin were compared following single and repeated applications over one week. RESULTS: The test results are reported as a percent change from baseline after each of the treatments. (See table above) CONCLUSIONS: A novel alcohol hand gel has been developed which offers the antimicrobial effectiveness of alcohol, the utility and time conservation of waterless application, and demonstrated long-lasting skin moisturization after single and repeated use.

handwash, surgical scrub, cup scrub, agar patch, repeat insult patch test, and primary skin irritation patch test. RESULTS: The studies afforded a comparison of the immediate, cumulative, and persistent antimicrobial activity of the formulations against resident and transient flora. The dermatological studies demonstrated the irritation and sensitization potential among the formulations. (See table below) CONCLUSIONS: New f o r m u l a r y t e c h n o l o g y has extended the safety and efficacy of alcohol-based formulations resulting in non-irritating, non-sensitizing hand rubs offering immediate, cumulative, and persistent antimicrobial activity. These formulations offer healthcare workers a safe, effective formulation to encourage handwashing compliance without compromising skin integrity and patient care.

A S S E S S M E N T OF T H E A C T M T Y OF AN ETHANOLCONTAINING H A N D GEL AGAINST NON-ENVELOPED VIRUSES USING THE FINGERPAD METHOD.

PhD, J. Newman, PhD Johnson & Johnson Medical, Arlington, TX; and R. Jones, BS, RM(AAM) Scientific & Regulatory Consultants, I n c , Columbia City, IN.

S. Sattar, PhD,* M. Abebe, MSc, Centre for Research on E n v i r o n m e n t a l Microbiology, University of Ottawa, Ottawa, ON; H. Jampani, PhD, J. Newman, PhD, Johnson & Johnson Medical, Inc., Arlington, TX; and R. Jones, BS, RM(AAM) Scientific and Regulatory Affairs, Inc., Columbia City, IN.

BACKGROUND: The history of the safety, effectiveness, and disadvantages of alcohol has been established for over 100 years. However through modern formulary technology, alcohol-based formulations for use as hand rubs and surgical scrubs may now offer the beneficial attributes previously reserved for soap-based formulations. METHODS: In vivo antimicrobial activity and skin irritation of the following alcohol-based formulations were measured: 1) 60% alcohol (PREVACARET M Antimicrobial Hand Gel), and 2) 62% alcohol rinse. Formulation attributes were compared using conventional tests: the healthcare personnel

BACKGROUND: Hands can spread many viruses in healthcare and other settings. The activity of handwash/ handrub agents against bacteria does not necessarily reflect their effectiveness against viruses. In spite of this, such agents are generally not tested against viruses. OBJECTIVE: To test an ethanol-containing hand gel for its activity against non-enveloped human pathogenic viruses using the fingerpad method (ASTM E1838-96). MATERIALS AND METHODS: Human rhinovirus-14 and the Wa strain of human rotavirus were tested using 12 subjects/virus. The product contained 60% ethanol (PRE-

ALCOHOL: NOVEL ANTIMICROBIAL TECHNOLOGY FOR P E R S I S T E N T A C T M T Y . H. Jampani, PhD,* A. Lee,

Test AGAR PATCH: Percent Inhibition in S. aureus after Application¢ CUP SCRUB: Percent Reduction in S. aureus after Application¢

1- - Statistically significant (p>0.05) - Compared to untreated control

Formula 1 2 1 2 1

Inoculum 2-3 x 102 cfu 1 xl04 cfu 1 xl06 cfu

1 Hour

3 Hours

97.93 t 00.00 90.56 t 24.28 1" 92.9 1"

96.10 135.67 1 70.2 1"

4 Hours 92,76 121,74 61,3 t

5 Hours 71,84 t 10.00 70.3 1-