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Aligned core–shell structured ultrafine composite fibers of PLLA–collagen for tendon scaffolding
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Abstracts / Journal of Controlled Release 172 (2013) e125–e149
better mechanical and thermal properties and improved electric conductivity; in fact the smaller, the better [2]. In this paper we have prepared fibers with a diameter as small as 5 nm (50 angstroms), as shown in Fig. 1. Polyvinyl alcohol (PVA) with a degree of polymerization of 1750 ± 50 was successfully fabricated into nanofibers via the traditional electrospinning process. Daughter charged jets were observed and fibers in the last cascade reach a diameter as small as a few nanometers. This might be the smallest artificial fiber possible, which might have excellent properties due to the nano-effect.
We report herein a facile and effective route to obtain well aligned, mechanically strong, and collagen-functionalized PLLA nanofibers in the configuration of core–sheath (i.e., PLLA–collagen) for tendon scaffolding. This was realized by employing a recently developed stable jet electrospinning to generate well aligned electrospun nanofibers [2]. As shown in Fig. 1A, by using a compound spinneret and formulating the viscoelasticity of the inner PLLA solution to form a stable jet, nonelectrospinnable dilute collagen solution was successfully coated onto the surface of the PLLA jet/fiber template. The core–shell structure of these composite fibers was verified by FTIR analysis (Fig. 1B). Wide angle X-ray diffraction (WAXD) results indicated a higher preferred crystalline orientation of thus made composite fibers (at 75.3%) than that (at 64.1%) from conventional coaxial electrospinning involving bending instability. Contact angle (CA) measurements revealed a favorable wettability with a CA value of 47.93 ± 2.09°, whereas the CA of electrospun PLLA fibers without collagen coating was 104.52 ± 4.09°. While the biological function of the biomimetic PLLA–collagen is still under evaluation, it is expected that current composite fibers of PLLA– collagen with favorable physicomechanical and biological characteristics will be of great interest in engineering tissues with anisotropic structural features, including tendon, ligament, blood vessels, and so on.
Fig. 1. SEM images of 10 wt% PVA nanofibers.
Keywords: fiber technology, electrospinning, minimal nanofiber, angstrom technology Acknowledgments The work is supported by PAPD (A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions), and the National Natural Science Foundation of China under Grant Nos. 10972053 and 10802021.
Fig. 1. Stable jet based coaxial electrospinning for making aligned core-shell nanofibers of PLLA-collagen (A), where the core-shell configuration was confirmed by FTIR spectra (B).
Keywords: stable jet electrospinning, poly(l-lactic acid), collagen, core–shell, tendon engineering
References [1] P. Gibson, H. Schreuder-Gibson, D. Rivin, Transport properties of porous membranes based on electrospun nanofibers, Colloid Surf. A 187 (2001) 469–481. [2] J.H. He, The smaller, the better: from the spider-spinning to bubble-electrospinning, Acta Phys. Pol. A 1 (2012) 254–256.
doi:10.1016/j.jconrel.2013.08.203
Acknowledgments This work was supported by the Pujiang Talent Programme (10PJ1400200), the National Natural Science Foundation of China (51073032), and the Fundamental Research Funds for the Central Universities (11D10540). References
Aligned core–shell structured ultrafine composite fibers of PLLA–collagen for tendon scaffolding Hongbin Tu, Min Bao, Qin Li, Biyun Li, Huihua Yuan, Yanzhong Zhang⁎ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, China E-mail addresses: [email protected] (H. Tu), [email protected] (Y. Zhang). From a biomimicking point of view, achieving unidirectionally oriented nanofiber assemblies for tendon scaffolding is highly desired. Efficacy of using aligned nanofibers for regulating tendon stem cell differentiation towards developing desirable engineered tendons has recently been elegantly demonstrated [1]. However, while many methods have been devised to render electrospun fibers in alignment, the current state-of-the-art fabrication still fails to deliver easy, rapid, effective, and continuous fiber-aligning capabilities. This continues to hinder mass scale manufacturability and practical applications of such kinds of electrospun fibers. Moreover, the preparation of aligned electrospun nanofibers with high mechanical strength and adequate biocompatibility also remains a challenge.
[1] Z. Yin, X. Chen, J.L. Chen, W.L. Shen, T.M.H. Nguyen, et al., The regulation of tendon stem cell differentiation by the alignment of nanofibers, Biomaterials 31 (2010) 2163–2175. [2] H.H. Yuan, B. Feng, H.J. Peng, et al., Electrically driven spinning of aligned ultrafine chitosan fibers, 2011 International Forum on Biomedical Textile Materials Proceedings, 2011, pp. 127–130.
doi:10.1016/j.jconrel.2013.08.204
The interaction of poly(ethylene glycol) with bovine serum albumin studied with low-field nuclear magnetic resonance Jiang Wu, Shengfu Chen⁎ State Key Laboratory of Chemical Engineering and Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China E-mail address: [email protected] (S. Chen). The stealth behavior of non-ionic hydrophilic PEG polymer has led to the design and synthesis of many PEG conjugates and PEGylated carriers for different drug-delivery applications [1]. Investigation of the interaction of proteins with PEG polymers in aqueous systems is very important, not only for understanding the way in which these polymers
Abstracts / Journal of Controlled Release 172 (2013) e125–e149
better mechanical and thermal properties and improved electric conductivity; in fact the smaller, the better [2]. In this paper we have prepared fibers with a diameter as small as 5 nm (50 angstroms), as shown in Fig. 1. Polyvinyl alcohol (PVA) with a degree of polymerization of 1750 ± 50 was successfully fabricated into nanofibers via the traditional electrospinning process. Daughter charged jets were observed and fibers in the last cascade reach a diameter as small as a few nanometers. This might be the smallest artificial fiber possible, which might have excellent properties due to the nano-effect.
We report herein a facile and effective route to obtain well aligned, mechanically strong, and collagen-functionalized PLLA nanofibers in the configuration of core–sheath (i.e., PLLA–collagen) for tendon scaffolding. This was realized by employing a recently developed stable jet electrospinning to generate well aligned electrospun nanofibers [2]. As shown in Fig. 1A, by using a compound spinneret and formulating the viscoelasticity of the inner PLLA solution to form a stable jet, nonelectrospinnable dilute collagen solution was successfully coated onto the surface of the PLLA jet/fiber template. The core–shell structure of these composite fibers was verified by FTIR analysis (Fig. 1B). Wide angle X-ray diffraction (WAXD) results indicated a higher preferred crystalline orientation of thus made composite fibers (at 75.3%) than that (at 64.1%) from conventional coaxial electrospinning involving bending instability. Contact angle (CA) measurements revealed a favorable wettability with a CA value of 47.93 ± 2.09°, whereas the CA of electrospun PLLA fibers without collagen coating was 104.52 ± 4.09°. While the biological function of the biomimetic PLLA–collagen is still under evaluation, it is expected that current composite fibers of PLLA– collagen with favorable physicomechanical and biological characteristics will be of great interest in engineering tissues with anisotropic structural features, including tendon, ligament, blood vessels, and so on.
Fig. 1. SEM images of 10 wt% PVA nanofibers.
Keywords: fiber technology, electrospinning, minimal nanofiber, angstrom technology Acknowledgments The work is supported by PAPD (A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions), and the National Natural Science Foundation of China under Grant Nos. 10972053 and 10802021.
Fig. 1. Stable jet based coaxial electrospinning for making aligned core-shell nanofibers of PLLA-collagen (A), where the core-shell configuration was confirmed by FTIR spectra (B).
Keywords: stable jet electrospinning, poly(l-lactic acid), collagen, core–shell, tendon engineering
References [1] P. Gibson, H. Schreuder-Gibson, D. Rivin, Transport properties of porous membranes based on electrospun nanofibers, Colloid Surf. A 187 (2001) 469–481. [2] J.H. He, The smaller, the better: from the spider-spinning to bubble-electrospinning, Acta Phys. Pol. A 1 (2012) 254–256.
doi:10.1016/j.jconrel.2013.08.203
Acknowledgments This work was supported by the Pujiang Talent Programme (10PJ1400200), the National Natural Science Foundation of China (51073032), and the Fundamental Research Funds for the Central Universities (11D10540). References
Aligned core–shell structured ultrafine composite fibers of PLLA–collagen for tendon scaffolding Hongbin Tu, Min Bao, Qin Li, Biyun Li, Huihua Yuan, Yanzhong Zhang⁎ State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, China E-mail addresses: [email protected] (H. Tu), [email protected] (Y. Zhang). From a biomimicking point of view, achieving unidirectionally oriented nanofiber assemblies for tendon scaffolding is highly desired. Efficacy of using aligned nanofibers for regulating tendon stem cell differentiation towards developing desirable engineered tendons has recently been elegantly demonstrated [1]. However, while many methods have been devised to render electrospun fibers in alignment, the current state-of-the-art fabrication still fails to deliver easy, rapid, effective, and continuous fiber-aligning capabilities. This continues to hinder mass scale manufacturability and practical applications of such kinds of electrospun fibers. Moreover, the preparation of aligned electrospun nanofibers with high mechanical strength and adequate biocompatibility also remains a challenge.
[1] Z. Yin, X. Chen, J.L. Chen, W.L. Shen, T.M.H. Nguyen, et al., The regulation of tendon stem cell differentiation by the alignment of nanofibers, Biomaterials 31 (2010) 2163–2175. [2] H.H. Yuan, B. Feng, H.J. Peng, et al., Electrically driven spinning of aligned ultrafine chitosan fibers, 2011 International Forum on Biomedical Textile Materials Proceedings, 2011, pp. 127–130.
doi:10.1016/j.jconrel.2013.08.204
The interaction of poly(ethylene glycol) with bovine serum albumin studied with low-field nuclear magnetic resonance Jiang Wu, Shengfu Chen⁎ State Key Laboratory of Chemical Engineering and Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China E-mail address: [email protected] (S. Chen). The stealth behavior of non-ionic hydrophilic PEG polymer has led to the design and synthesis of many PEG conjugates and PEGylated carriers for different drug-delivery applications [1]. Investigation of the interaction of proteins with PEG polymers in aqueous systems is very important, not only for understanding the way in which these polymers