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Allergy in Brucella infections
217
ABSTRACTS
In a small series, the author also demonstrated typical anaphylactic shock and autopsy findings in t.hree hypophysectomized rats sensitized with alum-precipitated ovalbumin. Of ten sensitized, nonoperated controls, none died, and six exhibited some evidences of shock.
Allergy in Brucella
Infections. Morales-Otero, Sot. Exper. Biol. & Med. 40: 100, 3939.
P., and Gonzal&,
L. If.:
Proc,.
Cutaneous hypersensitiveness to Brucelln and other immunologic reactions, such as agglutination, complemeut fixation, and opsonocytophagic index, were investigated among 212 milkers and cattle handlers in a region where endemic abortion in cat.tle was prevalent. One-tenth cubic rentimetar of a purified protein ant.igen prepared from UruceZZa and diluted with saline so that it contained 0.1 mg. nit.rogen per cubic centimeter was used for intracutaneoua testing. A reading was made in fort.y-eight hours. .4 positive reaction consisted of marked erythema and induration. As a rule, t.he area of edema was much larger than the erythematous area. (‘onstitutional reactions, manifested by general malaise and slight fever, occasionally occurred. Of the 212 cases tested, i (3.3 per cent) showed positive complement fixation, 5 (2.3 per cent) positive agglutination, 46 (21.7 per cent) positive opsonocytophagic reactions, and 63 (29.6 per ecntj showed cautaneous h.ypersensitiveness to Brucelh antigen. Of all the persons tested, 3.3 per cent had a history of undulent fever. Three of seven persons working in the nuthors ’ laboratory showed positive cutaneoux reactions. These three workers were the only ones in the series who had handled Bruceh cultures. They had never shown signs or symptoms of the disease. The results indicated that t.here was no correlation between cutaneous hypersensitiveness to Brucella. and other immunologic reactions to the same antigen. Contnct with infected material evidently resulted in the development. of a &ate of hypersensitiveness without clinicnl evidence of the d&ease. The cutaneous reaction to Brzlcella, therefore, does not necessarily indicate past or present infection, since hypersensitiveness may develop in persons who have had close contact with the infected material. The Blood Histamine During Anaphylactic Shock in the Horse and Calf. C. 1”., and Hester, H. H.: Am. J. Physiol. 127: Tl, 3039.
Code,
A modificntion of the Barsoum ard Gaddum technique was employed to study alterations in the histamine content of the blood during anaphylactic shock in the horse and calf. Two horses were sensit.ized by twelve daily intravenous injections of 45 C.C. of dog serum. Two other animals were sensitized by alternating intravenous and subcutaneous injections of from 40 to 50 C.C. of egg white administered daily. A shocking dose of from 100 to 600 C.C. of the specific ant.igen was administered The intravenous injection of 100 C.C. intravenously after a three-week intervnl. of dog serum in normal control horses produced no reaction. The intravenous injection of 500 C.C. of egg white in the normal animal produced a mild reaction with The anaphylactic a t.ransient negligible fall in the blood histamine content. symptoms in the horse consisted of increased peristalsis, mnrked sweating, and In one instance, anaphylaetic death occurred progressive respiratory dyspnea. within eight minutes following the injection of the shock dose of egg white. During anaphylactic shock the histamine content of the blood decreased in two animals as compared with normal control values, and in two instances it eompletely disappeared from the blood.
ABSTRACTS
In a small series, the author also demonstrated typical anaphylactic shock and autopsy findings in t.hree hypophysectomized rats sensitized with alum-precipitated ovalbumin. Of ten sensitized, nonoperated controls, none died, and six exhibited some evidences of shock.
Allergy in Brucella
Infections. Morales-Otero, Sot. Exper. Biol. & Med. 40: 100, 3939.
P., and Gonzal&,
L. If.:
Proc,.
Cutaneous hypersensitiveness to Brucelln and other immunologic reactions, such as agglutination, complemeut fixation, and opsonocytophagic index, were investigated among 212 milkers and cattle handlers in a region where endemic abortion in cat.tle was prevalent. One-tenth cubic rentimetar of a purified protein ant.igen prepared from UruceZZa and diluted with saline so that it contained 0.1 mg. nit.rogen per cubic centimeter was used for intracutaneoua testing. A reading was made in fort.y-eight hours. .4 positive reaction consisted of marked erythema and induration. As a rule, t.he area of edema was much larger than the erythematous area. (‘onstitutional reactions, manifested by general malaise and slight fever, occasionally occurred. Of the 212 cases tested, i (3.3 per cent) showed positive complement fixation, 5 (2.3 per cent) positive agglutination, 46 (21.7 per cent) positive opsonocytophagic reactions, and 63 (29.6 per ecntj showed cautaneous h.ypersensitiveness to Brucelh antigen. Of all the persons tested, 3.3 per cent had a history of undulent fever. Three of seven persons working in the nuthors ’ laboratory showed positive cutaneoux reactions. These three workers were the only ones in the series who had handled Bruceh cultures. They had never shown signs or symptoms of the disease. The results indicated that t.here was no correlation between cutaneous hypersensitiveness to Brucella. and other immunologic reactions to the same antigen. Contnct with infected material evidently resulted in the development. of a &ate of hypersensitiveness without clinicnl evidence of the d&ease. The cutaneous reaction to Brzlcella, therefore, does not necessarily indicate past or present infection, since hypersensitiveness may develop in persons who have had close contact with the infected material. The Blood Histamine During Anaphylactic Shock in the Horse and Calf. C. 1”., and Hester, H. H.: Am. J. Physiol. 127: Tl, 3039.
Code,
A modificntion of the Barsoum ard Gaddum technique was employed to study alterations in the histamine content of the blood during anaphylactic shock in the horse and calf. Two horses were sensit.ized by twelve daily intravenous injections of 45 C.C. of dog serum. Two other animals were sensitized by alternating intravenous and subcutaneous injections of from 40 to 50 C.C. of egg white administered daily. A shocking dose of from 100 to 600 C.C. of the specific ant.igen was administered The intravenous injection of 100 C.C. intravenously after a three-week intervnl. of dog serum in normal control horses produced no reaction. The intravenous injection of 500 C.C. of egg white in the normal animal produced a mild reaction with The anaphylactic a t.ransient negligible fall in the blood histamine content. symptoms in the horse consisted of increased peristalsis, mnrked sweating, and In one instance, anaphylaetic death occurred progressive respiratory dyspnea. within eight minutes following the injection of the shock dose of egg white. During anaphylactic shock the histamine content of the blood decreased in two animals as compared with normal control values, and in two instances it eompletely disappeared from the blood.