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Alternative immunoassays
ANALYTICAL
BIOCHEMISTRY
152,208
( 1986)
BOOK REVIEW Immunoassays. Edited by W. P. COLLINS,John Wiley & Sons, New York, 1985. 244 pp. $44.95
Alternative
This volume is a collection of articles by authors from various disciplines ranging from clinical biochemistry to endocrinology and pathology. While each of the authors has his or her own preferred approach to developing new immunoassays, it seems clear that there is a real impetus for alternatives to the presently used radioimmunoassays. The reasons often cited are a need for increased sensitivity, longer shelf life of reagents, and elimination of the hazards associated with handling and disposing of radioisotopes. There are several general chapters discussing the history and uses of immunoassays. There is a good discussion on the properties of monoclonal antibodies and the development of the two site immunomdiometric (IRMA) assay. The chapter on enzyme immunoassay reviews the various types of enzyme assaysthat have been developed and has a complete listing of current literature citations. The last half of the book is devoted to immunoassays in which the product measured is light. This includes bioand chemiluminescent immunoassays where the light
0003-2697186 $3.00 Copyright
Q 1986 by Academic Press, Inc. ,411rights0r reproduciion in any form reserved.
208
generated is a function of an enzymatic or chemical reaction or, alternatively, fluorescent or phosphorescent immunoassays where the labeled antigen or antibody is excited with light. The various assaysthat have already been developed are discussed in some detail and compared with RIAs. There are critical discussions of the advantages and disadvantages of each of the labels used and of the type of instrumentation required. There is a chapter on chemihtminescence energy transfer which is an interesting experimental approach to immunoassays.The two chapters on fluorescent immunoassays offer strong support for the use of time-resolved fluorometry in developing new and very sensitive assays. The last chapter is a theoretical discussion of the various factors which affect different immunoassay strategies and the potential limits of sensitivity. This collection of papers is a current and concise review of the possible alternatives to radioimmunoassays and should be very useful as an introduction to those who are entering the field, as well as to investigators already using immunoassays. MARLENE
DELUCA
BIOCHEMISTRY
152,208
( 1986)
BOOK REVIEW Immunoassays. Edited by W. P. COLLINS,John Wiley & Sons, New York, 1985. 244 pp. $44.95
Alternative
This volume is a collection of articles by authors from various disciplines ranging from clinical biochemistry to endocrinology and pathology. While each of the authors has his or her own preferred approach to developing new immunoassays, it seems clear that there is a real impetus for alternatives to the presently used radioimmunoassays. The reasons often cited are a need for increased sensitivity, longer shelf life of reagents, and elimination of the hazards associated with handling and disposing of radioisotopes. There are several general chapters discussing the history and uses of immunoassays. There is a good discussion on the properties of monoclonal antibodies and the development of the two site immunomdiometric (IRMA) assay. The chapter on enzyme immunoassay reviews the various types of enzyme assaysthat have been developed and has a complete listing of current literature citations. The last half of the book is devoted to immunoassays in which the product measured is light. This includes bioand chemiluminescent immunoassays where the light
0003-2697186 $3.00 Copyright
Q 1986 by Academic Press, Inc. ,411rights0r reproduciion in any form reserved.
208
generated is a function of an enzymatic or chemical reaction or, alternatively, fluorescent or phosphorescent immunoassays where the labeled antigen or antibody is excited with light. The various assaysthat have already been developed are discussed in some detail and compared with RIAs. There are critical discussions of the advantages and disadvantages of each of the labels used and of the type of instrumentation required. There is a chapter on chemihtminescence energy transfer which is an interesting experimental approach to immunoassays.The two chapters on fluorescent immunoassays offer strong support for the use of time-resolved fluorometry in developing new and very sensitive assays. The last chapter is a theoretical discussion of the various factors which affect different immunoassay strategies and the potential limits of sensitivity. This collection of papers is a current and concise review of the possible alternatives to radioimmunoassays and should be very useful as an introduction to those who are entering the field, as well as to investigators already using immunoassays. MARLENE
DELUCA