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Amniotic fluid renal progenitors and renal extracellular matrix: a new approach for kidney regeneration
UROLOGY natural or biodegradable scaffold with cells and growth factors could be a new approach to discover an alternative tool for replacing significantly impaired or non-functional kidney tissue. Our laboratory has previously reported that human amniotic fluid (AF) presents a new source of renal progenitor cells.
Pre-biopsy multi-parametric magnetic resonance studies correlate with Gleason score on prostate biopsy Rajeev Kumar, MBBS, Durgesh K Dwivedi, MSc, Girdhar S Bora, MCh, Sanjay Thulkar, Sanjay Sharma, MD, FRCR, DNB, Sidhartha Dattagupta, NR Jagannathan, PhD All India Institute of Medical Sciences, New Delhi, India
METHODS: A subpopulation of renal progenitor cells expressing CD24 and OB-cadherin were isolated from AF. Using a detergent-enzymatic method we produced mouse decellularized whole kidney extracellular matrix (ECM). Mouse kidney ECM was seeded with renal progenitor cells, implanted into the kidney of nude mice, and harvested at one month to look for structure and phenotypic expression.
INTRODUCTION: Stratification of prostate cancer on the basis of disease aggressiveness is a current clinical challenge. We evaluated the ability of pre-biopsy magnetic resonance spectroscopic imaging (MRSI) and diffusion weighted imaging (DWI) in stratifying tumor aggressiveness by correlating their values with biopsy Gleason score.
RESULTS: One month after surgery, implanted ECM in situ showed invasion of vessels (angiogenesis) and renal tubular-like structures. In addition to seeded scaffolds configuring into 3 dimensional renal structures, cells positive for markers associated with several essential renal cell types, such as mesangial, podocytes and tubular cells were detected.
METHODS: This institutional ethics committee approved study, conducted between 2009 and 2012, included 120 men who underwent pre-prostate biopsy multiparametric MR for suspected cancer. Patients with cancer were stratified by Gleason score (GS 6, GS¼7, and GS8). Metabolite ratio [Cit/(Cho+Cr)] and apparent diffusion coefficient (ADC) were calculated for voxels which corresponded to biopsy sites positive for malignancy. Values of MR data were correlated with the Gleason score.
CONCLUSIONS: These results suggest that renal progenitor cells from AF seeded into renal ECM could represent a unique investigational approach for kidney regeneration that may be used to augment or replace damaged or compromised kidney tissue from either congenital or chronic disease.
RESULTS: 26 men (21.7%) had adenocarcinoma prostate. Metabolite ratio for GS 6, GS¼7, and GS8 groups was 0.32 0.16, 0.20 0.06 and 0.13 0.06 respectively. These values were statistically different (p<0.05) between GS 6 and GS¼7, and GS 6 and GS8. The ADC value (x10-3 mm2/s) for these groups was 0.99 0.08, 0.86 0.11 and 0.69 0.12 respectively. These values were statistically different (p<0.05) between GS 6 and GS¼7, GS 6 and GS8, and GS¼7 and GS8. Metabolite ratios showed a strong negative correlation with GS (r ¼ -0.66, p¼0.0) as did ADC values (r ¼ -0.74, p¼0.0). To differentiate GS 6 from GS8 cancer, area under curve (AUC) for metabolite ratio was 84% (95% CI: 0.66-0.97) and 89% for ADC (95% CI: 0.75-0.99).
Predictors of development of life threatening complications (Clavien IV) in patients with necrotizing soft tissue infections of the perineum Efstathios Karamanos, MD, Naveen Kachroo, MD, PhD, Sriram Eleswarapu, MD, PhD, Kelly J Rosso, MD, Aamir Siddiqui, MD, FACS, Ilan S Rubinfeld, MD, FACS Henry Ford Hospital, Detroit, MI INTRODUCTION: Necrotizing soft tissue infection (NSTI) of the perineum presents a unique challenge to the surgeon and is associated with high mortality and complication rates. Outcomes are highly reliable on prompt recognition and timely aggressive surgical debridement. This study seeks to identify predictors of life threatening complications in this study population.
CONCLUSIONS: Pre-biopsy multiparametric MR correlates with Gleason score and can stratify prostate cancer aggressiveness. Amniotic fluid renal progenitors and renal extracellular matrix: a new approach for kidney regeneration Roger E De Filippo, MD, FACS, Ilenia Zanusso, PhD, Stefano Da Sacco, PhD, Scott Leslie, MD, Astgik Petrosyan, BS, Kevin V Lemley, MD, PhD, Laura Perin, PhD University of Southern California and Children’s Hospital Los Angeles, Los Angeles, CA
METHODS: Patients with NSTI of the perineum (ICD-9 code: 608.83) from 2005 to 2012 were identified using the American College of Surgeons National Surgical Quality Improvement Program database. Complications were stratified based on the Clavien classification. Clavien IV (life threatening complications requiring ICU management) were identified. Univariate analyses were performed to identify clinical characteristics associated with high incidence of Clavien IV complications post operatively.
INTRODUCTION: Congenital abnormalities of the kidney and urinary tract (CAKUT) still remain a significant contributing factor for chronic kidney disease and end organ failure. Current treatments are directed towards slowing progression before dialysis and/or transplantation become necessary. The scarcity of allografts, cost, and side effects, have prompted efforts at investigating new tools for kidney regeneration. Tissue engineering that combines a
ª 2014 by the American College of Surgeons Published by Elsevier Inc.
RESULTS: A total of 235 underwent debridement for NSTI of the perineum. Overall mortality was 9.4%, mean hospital length of stay (HLOS) was 16.9 days and 25.5% developed Clavien IV complications. Increasing frailty index was associated with an increasing incidence of Clavien IV complications (p<0.001). DM on insulin,
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http://dx.doi.org/10.1016/j.jamcollsurg.2014.07.530 ISSN 1072-7515/14
Pre-biopsy multi-parametric magnetic resonance studies correlate with Gleason score on prostate biopsy Rajeev Kumar, MBBS, Durgesh K Dwivedi, MSc, Girdhar S Bora, MCh, Sanjay Thulkar, Sanjay Sharma, MD, FRCR, DNB, Sidhartha Dattagupta, NR Jagannathan, PhD All India Institute of Medical Sciences, New Delhi, India
METHODS: A subpopulation of renal progenitor cells expressing CD24 and OB-cadherin were isolated from AF. Using a detergent-enzymatic method we produced mouse decellularized whole kidney extracellular matrix (ECM). Mouse kidney ECM was seeded with renal progenitor cells, implanted into the kidney of nude mice, and harvested at one month to look for structure and phenotypic expression.
INTRODUCTION: Stratification of prostate cancer on the basis of disease aggressiveness is a current clinical challenge. We evaluated the ability of pre-biopsy magnetic resonance spectroscopic imaging (MRSI) and diffusion weighted imaging (DWI) in stratifying tumor aggressiveness by correlating their values with biopsy Gleason score.
RESULTS: One month after surgery, implanted ECM in situ showed invasion of vessels (angiogenesis) and renal tubular-like structures. In addition to seeded scaffolds configuring into 3 dimensional renal structures, cells positive for markers associated with several essential renal cell types, such as mesangial, podocytes and tubular cells were detected.
METHODS: This institutional ethics committee approved study, conducted between 2009 and 2012, included 120 men who underwent pre-prostate biopsy multiparametric MR for suspected cancer. Patients with cancer were stratified by Gleason score (GS 6, GS¼7, and GS8). Metabolite ratio [Cit/(Cho+Cr)] and apparent diffusion coefficient (ADC) were calculated for voxels which corresponded to biopsy sites positive for malignancy. Values of MR data were correlated with the Gleason score.
CONCLUSIONS: These results suggest that renal progenitor cells from AF seeded into renal ECM could represent a unique investigational approach for kidney regeneration that may be used to augment or replace damaged or compromised kidney tissue from either congenital or chronic disease.
RESULTS: 26 men (21.7%) had adenocarcinoma prostate. Metabolite ratio for GS 6, GS¼7, and GS8 groups was 0.32 0.16, 0.20 0.06 and 0.13 0.06 respectively. These values were statistically different (p<0.05) between GS 6 and GS¼7, and GS 6 and GS8. The ADC value (x10-3 mm2/s) for these groups was 0.99 0.08, 0.86 0.11 and 0.69 0.12 respectively. These values were statistically different (p<0.05) between GS 6 and GS¼7, GS 6 and GS8, and GS¼7 and GS8. Metabolite ratios showed a strong negative correlation with GS (r ¼ -0.66, p¼0.0) as did ADC values (r ¼ -0.74, p¼0.0). To differentiate GS 6 from GS8 cancer, area under curve (AUC) for metabolite ratio was 84% (95% CI: 0.66-0.97) and 89% for ADC (95% CI: 0.75-0.99).
Predictors of development of life threatening complications (Clavien IV) in patients with necrotizing soft tissue infections of the perineum Efstathios Karamanos, MD, Naveen Kachroo, MD, PhD, Sriram Eleswarapu, MD, PhD, Kelly J Rosso, MD, Aamir Siddiqui, MD, FACS, Ilan S Rubinfeld, MD, FACS Henry Ford Hospital, Detroit, MI INTRODUCTION: Necrotizing soft tissue infection (NSTI) of the perineum presents a unique challenge to the surgeon and is associated with high mortality and complication rates. Outcomes are highly reliable on prompt recognition and timely aggressive surgical debridement. This study seeks to identify predictors of life threatening complications in this study population.
CONCLUSIONS: Pre-biopsy multiparametric MR correlates with Gleason score and can stratify prostate cancer aggressiveness. Amniotic fluid renal progenitors and renal extracellular matrix: a new approach for kidney regeneration Roger E De Filippo, MD, FACS, Ilenia Zanusso, PhD, Stefano Da Sacco, PhD, Scott Leslie, MD, Astgik Petrosyan, BS, Kevin V Lemley, MD, PhD, Laura Perin, PhD University of Southern California and Children’s Hospital Los Angeles, Los Angeles, CA
METHODS: Patients with NSTI of the perineum (ICD-9 code: 608.83) from 2005 to 2012 were identified using the American College of Surgeons National Surgical Quality Improvement Program database. Complications were stratified based on the Clavien classification. Clavien IV (life threatening complications requiring ICU management) were identified. Univariate analyses were performed to identify clinical characteristics associated with high incidence of Clavien IV complications post operatively.
INTRODUCTION: Congenital abnormalities of the kidney and urinary tract (CAKUT) still remain a significant contributing factor for chronic kidney disease and end organ failure. Current treatments are directed towards slowing progression before dialysis and/or transplantation become necessary. The scarcity of allografts, cost, and side effects, have prompted efforts at investigating new tools for kidney regeneration. Tissue engineering that combines a
ª 2014 by the American College of Surgeons Published by Elsevier Inc.
RESULTS: A total of 235 underwent debridement for NSTI of the perineum. Overall mortality was 9.4%, mean hospital length of stay (HLOS) was 16.9 days and 25.5% developed Clavien IV complications. Increasing frailty index was associated with an increasing incidence of Clavien IV complications (p<0.001). DM on insulin,
e55
http://dx.doi.org/10.1016/j.jamcollsurg.2014.07.530 ISSN 1072-7515/14