An enzyme-immuno-assay to detect myelin basic protein (MBP) in CSF and its clinical significance

131

SCIENTIFIC MEETING

OF THE

THURSDAYTTHAPRIL

1983

AMSTERDAMSCHE

AN ENZYME-IMMUNO-ASSAY CLINICAL SIGNIFICANCE

TO DETECT

NEUROLOGENVEREENIGING

MYELIN

HELD

BASIC PROTEIN

IN AMSTERDAM

ON

(MBP) IN CSF AND ITS

J.O.MISPELBLOM BEYER,J.C.KOETSIER.G.J.VAN KAMP and L.LUYENDIJK(AMSTERDAM)

Meylin basic protein is the most highly characterized of the myelin proteins. It comprises 30 percent of CNS myelin proteins. and is a linear polypeptide of approximately 170 amino acid residues with a molecular weight of 18.600 and an isoelectric point greater than 10.5. An enzyme-immuno-assay was developed for the detection of this protein in CSF. and applied to the analysis of CSF from different categories of neurological patients. The sensitivity of the test was less than I ng/ml. Higher values were found in CSF from patients with active multiple sclerosis (exacerbations and chronic progressive course), infectious diseases of the central nervous system. traumatic brain damage and cerebral infarction. Those findings are compatible with the results of other investigators. The test can be helpful in the diagnosis of neurological diseases, in which active myelin breakdown occurs.

CEREBELLOPONTINE ARTERY.

ANGLE

TUMOURS

AND THE ANTERIOR

INFERIOR

CEREBELLAR

J.J. HEIMANs. A.H.M.LOHMAN.J.VALK.J.H.A. v. D.DRIFT.(AMSTERDAM)

The anterior inferior cerebellar artery (AICA) originates from the proximal part of the basilar artery and usually traverses the cerebellopontine angle towards the lateral aspect of the cerebellar hemisphere. In the vicinity of the internal auditory meatus the artery may have close relations with the facial and vestibulocochlear nerves. Since long it has been recognized that damage to the AICA may occur as a complication of cerebellopontine angle surgery. Such damage can subsequently result in lateral pontine infarction. Therefore, it is important to have a detailed knowledge about the variations in course and distribution of the AICA. High resolution axial and coronal computerized tomography and oxygen CT cisternography are nowadays the methods of choice in the diagnosis of cerebellopontine angle tumours. Pre-operative angiographtc analysis can reveal the configuration of major arteries, such as the AICA. and. eventuelly. displacements of vessels. We studied 20 anatomical specimens. 100 normal vertebral angiograms and 20 vertebral angiograms of patients suffering from cerebellopontine angle tumours. Four main configurations of the AICA were distinguished. The type I AICA is short and does not reach further lateral than the cerebellopontine angle. The type II AICA usually forms a loop in the cerebellopontine angle and continues its course laterally in the great horizontal fissure towards the lateral border of the cerebellar hemisphere. In the type III AICA the artery also courses laterally, but, in addition, a caudomedial branch is present. which originates from the proximal part of the artery. This branch takes over part of or the whole area of supply of the posterior inferior cerebellar artery (PICA). In the type IV AICA there is only a caudomedial branch and no laterally coursing segment of the AICA can be identified. In types III and IV the PICA may be small or absent and in those cases lateral pontine infarction as a complication of cerebellopontine anglesurgery is more likely to occur than in cases in which types I and II AICA’s are present, THE NUCLEAR MAGNETIC J.VAI_K(ABCOUDE)

No abstract

available.

RESONANCE

THEORIE

IN CLINICAL

USE