An Experimental Study of Salivary Tumor Models in Mice

Oral Abstract Session 3 range; ⬎2 g in 2 min). The USW was measured by spitting saliva into a cup under unstimulated and resting conditions (normal range; ⬎1.5 mL in 15 min). Method of Data Analysis: The statistical significance of differences between the groups was determined by unpaired Student’s t test and Pearson’s correlation coefficients, and p values less than 0.05 were considered to be significant. Results: Both of the SWS and the UWS were decreased in 14 of the 16 SS patients, and significant correlation between the SWS and the UWS was observed in the SS patients. On the other hand, the SWS was not decreased in all of the 14 non-SS patients, whereas the UWS was decreased in 12 of the 14 non-SS patients. Any apparent correlation was not observed between the SWS and the UWS in the non-SS patients. Conclusion: The patients with drug-induced and neurotical dry mouth usually complain of dry mouth under resting conditions. The results obtained in this study suggest that the measurement of the UWS as well as the SWS is useful in the diagnosis of dry mouth, especially drug-induced and neurotical dry mouth. References C Vitali1, S Bombardieri1, R Jonsson2, H M Moutsopoulos3, E L Alexander4, S E Carsons5, T E Daniels6, P C Fox7, R I Fox8, S S Kassan9, S R Pillemer10, N Talal11 and M H Weisman12 and the European Study Group on Classification Criteria for Sjo ¨ gren’s Synd AnneMarie LyngePedersen*, AllanBardow and BirgitteNauntofte. Salivary changes and dental caries as potential oral markers of autoimmune salivary gland dysfunction in primary Sjo ¨ gren’s syndrome. BMC Clinical Pathology 2005, 5:4

An Experimental Study of Salivary Tumor Models in Mice Young-Wook Park, DDS, MSD, PhD, Gangneung Daehangno 120, Gangneung, Gangwon-do, 210-702, Korea (Kim HS; Kang HJ) Statement: Adenoid cystic carcinoma (ACC) is a relatively rare tumor that arises in glandular tissues of the head and neck region and sometimes has a protracted clinical course with perineural invasion and delayed occurrence of distant lung metastases. Treatment failure of salivary ACC is most often associated with perineural and hematogenous tumor spread. However, very little is known about the cellular and molecular mechanisms of perineural invasion and hematogenous distant metastasis. This study was designed to develop an orthotopic tumor model of parotid adenoid cystic carcinomas in athymic nude mice. Materials and Methods: A melanoma cell line was injected into the parotid gland of athymic mice to determine whether such implantation was technically feasible. The parotid ACC cell line was then injected into the parotid gland or the subcutaneous tissue of athymic mice at various concentrations of tumor cells, and the mice were thereafter followed for development of tumor nodules. The tumors 35.e2

were examined histopathologically for perineural invasion and regional or distant lung metastasis. We used an oral squamous cell carcinoma cell line as a control. Dr Jeffrey N. Myers (Department of Head and Neck surgery, University of Texas M.D. Anderson Cancer Center) kindly donated all cell lines. For this study, the exact logistic regression model was used to assess tumorigenicity and the effects of orthotopic (parotid) versus ectopic (subcutis) tumor development and metastases. The Ps for the location effect were evaluated against an ␣ significance level of 0.05. Method of Data Analysis: n/a Results: Implantation of tumor (melanoma) cell suspension into the parotid gland of nude mice was technically feasible and resulted in the formation of parotid tumors. A parotid ACC cell line, ACC3 did not demonstrate significantly higher tumorigenicity, but showed significantly higher lung metastatic potential in the parotid gland compared to the subcutis. By contrast, the mucosal squamous cell carcinoma cell line did not show significantly higher lung metastatic potential compared to the subcutis. Perineural ACC tumor, from cells established in the parotid gland, needs further study. Conclusion: An orthotopic tumor model of salivary ACC in athymic nude mice was successfully developed that closely mimics the clinical findings in human salivary ACC. This model should facilitate improved understanding of the cellular and molecular mechanisms of tumorigenisis and metastasis of salivary ACC and aid in the development of targeted molecular therapies for salivary ACC. Funding Source: This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea. (A060174) References Myers JN, Holsinger FC, Jasser SA, Bekele BN, Fidler IJ: An orthotopic nude mouse model of oral tongue squamous cell carcinoma. Clin Cancer Res 2002;8:293-298 Kim S, Park YW, Schiff BA, Doan DD, Yazici Y, Jasser SA et al: An orthotopic model of anaplastic thyroid carcinoma. Clin Cancer Res 2002;8:293-298

DNA-Aneuploidy in Oral Cancer Torsten W. Remmerbach, DDS, MOM, PhD, DDSc, PMB 50 GCMC, Bundall, QLD, 9726, Australia (Boecking A) Statement: The aneuploidy-cancer theory proposes that cancer is caused by the abnormal dosage of thousands of normal genes. This is generated by the gain or loss of specific chromosomes or segments of chromosomes; alias aneuploidy. The theory predicts that chromosomal and genetic instability is proportional to the degree of aneuploidy (Duesberg et al, 2004). Therefore an abnormal DNA-Content of epithelial cells (alias the degrees of DNA-Aneuploidy) was determined by measuring the integrated optical density of abnormal keratinoAAOMS • 2007