An improved method for long-term storage of colony filters

~'~ECHNICAL

Reannealing o f ~

~-~IPS

he.eroduplexes generated ~ g

The polymeraSe chain reaction (PCR) has ~ e n ~

PCR-mediated genetic tsotyptng

m the investigation of the three-member murtne gene family coding

lengths (253,: 236:and: 245 bp) whb=~ can ~ : 1

as a ~

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a ~ found the~ b a n ~ when a m i n i m ~ t ~ t ~ genomic Clones was u ~ d a s a extra b a n ~ were a i m the MR

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1 74

FIG~

genes a~: amptified by

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t~:~N~E t Wenger, Rill, and Nielsen~ PJ: Eur J, I m m u n o l ( i n p r e ~ )

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ContNbuted ~ RMand n. Wenger a ~ Peter J. Nielsen; Max Planck Instit~ fft r Immunbtologie, Stabeueg D- 7800. FRG. ...... .....

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a~ ph

~FERENcE

.

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~n), Co~dsp,u,g Co~bu~d ~ Kla~ ~ ~m ~ a ~ U~n~cb ~ t ~ o l t b a ~ , ~rcb), ~ (artS) of ~ , ~43oo ~ssen, FRG.

TIGJLNE 1991 VOL. 7 NO. 6

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